HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. Science (0036-8075) 1999 imex curation Taylor D.R., Shi S.T., Romano P.R., Barber G.N., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Taylor D.R., Shi S.T., Romano P.R., Barber G.N., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu Accepted 2013-SEP-18 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Science (0036-8075) 1999 imex curation Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. Science (0036-8075) 1999 imex curation Taylor D.R., Shi S.T., Romano P.R., Barber G.N., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Taylor D.R., Shi S.T., Romano P.R., Barber G.N., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu Accepted 2013-SEP-18 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Science (0036-8075) 1999 imex curation Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. Inhibition of the interferon-inducible protein kinase PKR by HCV E2 protein. Science (0036-8075) 1999 imex curation Taylor D.R., Shi S.T., Romano P.R., Barber G.N., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down tag visualisation tag visualisation Taylor D.R., Shi S.T., Romano P.R., Barber G.N., Lai M.M. Virus - Publications including interactions involving viral proteins michlai@hsc.usc.edu Accepted 2013-SEP-18 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Science (0036-8075) 1999 imex curation p27958-pro_0000037570 Envelope glycoprotein E2 protein protein HCV77 Hepatitis C virus genotype 1a (isolate H77) ETHVTGGNAGRTTAGLVGLLTPGAKQNIQLINTNGSWHINSTALNCNESLNTGWLAGLFYQHKFNSSGCPERLASCRRLTDFAQGWGPISYANGSGLDERPYCWHYPPRPCGIVPAKSVCGPVYCFTPSPVVVGTTDRSGAPTYSWGANDTDVFVLNNTRPPLGNWFGCTWMNSTGFTKVCGAPPCVIGGVGNNTLLCPTDCFRKYPEATYSRCGSGPRITPRCMVDYPYRLWHYPCTINYTIFKVRMYVGGVEHRLEAACNWTRGERCDLEDRDRSELSPLLLSTTQWQVLPCSFTTLPALSTGLIHLHQNIVDVQYLYGVGSSIASWAIKWEYVVLLFLLLADARVCSCLWMMLLISQAEA 384 746 9243E2103B6CCA2D e2ak2_human Interferon-induced, double-stranded RNA-activated protein kinase Interferon-inducible RNA-dependent protein kinase Eukaryotic translation initiation factor 2-alpha kinase 2 Protein kinase RNA-activated p68 kinase P1/eIF-2A protein kinase EIF2AK2 PKR PRKR Tyrosine-protein kinase EIF2AK2 protein protein human Homo sapiens Human MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC DIP protein P19525 original sequence version: 164 DIP protein P19525-1 original sequence version: 177 DIP protein P19525-1 original sequence version: 178 DIP protein P19525-1 original sequence version: 170 DIP protein P19525 original sequence version: 153 815AD83ACAB45DA3 e2-pkr-1 3 E2 4 unspecified role unspecified role prey prey region mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:07:15 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'pephd mutation' 35s tag 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PKR 5 unspecified role unspecified role bait bait his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2A pkr-2 2 PKR 5 putative self putative self putative self putative self human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. phosphorylation phosphorylation reaction phosphorylation true Fig. 2C e2-pkr-5 1 E2 4 unspecified role unspecified role neutral component neutral component PKR 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3H E2L (E2 with the leader peptide) pkr-3 2 PKR 5 putative self putative self putative self putative self ecoli Escherichia coli (strain K12) phosphorylation phosphorylation reaction phosphorylation true Fig. 2D eif2ak2-p27958_pro-1 2 PKR 5 putative self putative self putative self putative self ecoli Escherichia coli (strain K12) 4 inhibitor inhibitor unspecified role unspecified role phosphorylation phosphorylation reaction phosphorylation Fig. 2D P27958-PRO_0000037570 decreases activity of P19525