IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Cell (0092-8674) 2008 imex curation Wang Z., Edwards JG., Riley N., Provance DW., Karcher R., Li XD., Davison IG., Ikebe M., Mercer JA., Kauer JA., Ehlers MD. Synapse - Interactions of proteins with an established role in the presynapse. Colocalisations involving GluR1 could not be annotated as source of GluR1 could not be traced - dead end reference. ehlers@neuro.duke.edu 16-Dec-2008: Contacted by JYOTI. jyoti Only protein-protein interactions 7 rat-hippocampus Rattus norvegicus hippocampal neurons hippocampus Hippocampus [Brain hippocampus] fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Wang Z., Edwards JG., Riley N., Provance DW., Karcher R., Li XD., Davison IG., Ikebe M., Mercer JA., Kauer JA., Ehlers MD. Synapse - Interactions of proteins with an established role in the presynapse. Colocalisations involving GluR1 could not be annotated as source of GluR1 could not be traced - dead end reference. ehlers@neuro.duke.edu Accepted 2008-DEC-27 by JYOTI. 16-Dec-2008: Contacted by JYOTI. Cell (0092-8674) 2008 jyoti Only protein-protein interactions 7 imex curation Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Cell (0092-8674) 2008 imex curation Wang Z., Edwards JG., Riley N., Provance DW., Karcher R., Li XD., Davison IG., Ikebe M., Mercer JA., Kauer JA., Ehlers MD. Synapse - Interactions of proteins with an established role in the presynapse. Colocalisations involving GluR1 could not be annotated as source of GluR1 could not be traced - dead end reference. ehlers@neuro.duke.edu 16-Dec-2008: Contacted by JYOTI. jyoti Only protein-protein interactions 7 rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang Z., Edwards JG., Riley N., Provance DW., Karcher R., Li XD., Davison IG., Ikebe M., Mercer JA., Kauer JA., Ehlers MD. Synapse - Interactions of proteins with an established role in the presynapse. Colocalisations involving GluR1 could not be annotated as source of GluR1 could not be traced - dead end reference. ehlers@neuro.duke.edu Accepted 2008-DEC-27 by JYOTI. 16-Dec-2008: Contacted by JYOTI. Anti-Rab11 identified as anti-Rab11a in PMID: 12376546 Cell (0092-8674) 2008 jyoti Only protein-protein interactions 7 imex curation Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Cell (0092-8674) 2008 imex curation Wang Z., Edwards JG., Riley N., Provance DW., Karcher R., Li XD., Davison IG., Ikebe M., Mercer JA., Kauer JA., Ehlers MD. Synapse - Interactions of proteins with an established role in the presynapse. Colocalisations involving GluR1 could not be annotated as source of GluR1 could not be traced - dead end reference. ehlers@neuro.duke.edu 16-Dec-2008: Contacted by JYOTI. jyoti Only protein-protein interactions 7 rat-hippocampus Rattus norvegicus hippocampal neurons hippocampus Hippocampus [Brain hippocampus] fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Wang Z., Edwards JG., Riley N., Provance DW., Karcher R., Li XD., Davison IG., Ikebe M., Mercer JA., Kauer JA., Ehlers MD. Synapse - Interactions of proteins with an established role in the presynapse. Colocalisations involving GluR1 could not be annotated as source of GluR1 could not be traced - dead end reference. ehlers@neuro.duke.edu Accepted 2008-DEC-27 by JYOTI. 16-Dec-2008: Contacted by JYOTI. Cell (0092-8674) 2008 jyoti Only protein-protein interactions 7 imex curation Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Cell (0092-8674) 2008 imex curation Wang Z., Edwards JG., Riley N., Provance DW., Karcher R., Li XD., Davison IG., Ikebe M., Mercer JA., Kauer JA., Ehlers MD. Synapse - Interactions of proteins with an established role in the presynapse. Colocalisations involving GluR1 could not be annotated as source of GluR1 could not be traced - dead end reference. ehlers@neuro.duke.edu 16-Dec-2008: Contacted by JYOTI. jyoti Only protein-protein interactions 7 in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Wang Z., Edwards JG., Riley N., Provance DW., Karcher R., Li XD., Davison IG., Ikebe M., Mercer JA., Kauer JA., Ehlers MD. Synapse - Interactions of proteins with an established role in the presynapse. Colocalisations involving GluR1 could not be annotated as source of GluR1 could not be traced - dead end reference. ehlers@neuro.duke.edu Accepted 2008-DEC-27 by JYOTI. 16-Dec-2008: Contacted by JYOTI. Cell (0092-8674) 2008 jyoti Only protein-protein interactions 7 imex curation Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Myosin Vb mobilizes recycling endosomes and AMPA receptors for postsynaptic plasticity. Cell (0092-8674) 2008 imex curation Wang Z., Edwards JG., Riley N., Provance DW., Karcher R., Li XD., Davison IG., Ikebe M., Mercer JA., Kauer JA., Ehlers MD. Synapse - Interactions of proteins with an established role in the presynapse. Colocalisations involving GluR1 could not be annotated as source of GluR1 could not be traced - dead end reference. ehlers@neuro.duke.edu 16-Dec-2008: Contacted by JYOTI. jyoti Only protein-protein interactions 7 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang Z., Edwards JG., Riley N., Provance DW., Karcher R., Li XD., Davison IG., Ikebe M., Mercer JA., Kauer JA., Ehlers MD. Synapse - Interactions of proteins with an established role in the presynapse. Colocalisations involving GluR1 could not be annotated as source of GluR1 could not be traced - dead end reference. Source of RAB11FIP2 taken from PMID: 15173169. ehlers@neuro.duke.edu Accepted 2008-DEC-27 by JYOTI. 16-Dec-2008: Contacted by JYOTI. Cell (0092-8674) 2008 jyoti Only protein-protein interactions 7 imex curation atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) dlg4_rat Disks large homolog 4 Dlg4 Dlgh4 Postsynaptic density protein 95 Synapse-associated protein 90 Psd95 protein protein rat Rattus norvegicus (Rat) Rat MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL DIP protein P31016 original sequence version: 86 DIP protein P31016-1 original sequence version: 148 7922D4E8E0F9AD85 myo5b_rat Unconventional myosin-Vb Myosin heavy chain myr 6 Myo5b protein protein rat Rattus norvegicus (Rat) Rat MTYSELYSRYTRVWIPDPDEVWRSAELTKDYKDGDESLQLRLEDDTILDYPIDVQNNQVPFLRNPDILVGENDLTALSHLHEPAVLHNLKVRFLESNHIYTYCGIVLVAINPYEQLPIYGQDVIYAYSGQNMGDMDPHIFAVAEEAYKQMARDEKNQSIIVSGESGAGKTVSAKYAMRYFATVGGSASDTNIEEKVLASSPIMEAIGNAKTTRNDNSSRFGKYIEIGFDKKYHIIGANMRTYLLEKSRVVFQADDERNYHIFYQLCAAASLPEFKELALTCAEDFFYTAHGGNTTIEGVDDAEDFEKTRQALTLLGVRESHQISIFKIIASILHLGSVEIQAERDGDSCSISPQDEHLSNFCRLLGIEHSQMEHWLCHRKLVTTSETYVKTMSLQQVVNARNALAKHIYAQLFSWIVEHINKALQTSLKQHSFIGVLDIYGFETFEINSFEQFCINYANEKLQQQFNSHVFKLEQEEYMKEQIPWTLIDFYDNQPCIDLIEAKLGILDLLDEECKVPKGTDQNWAQKLYERHSNSQHFQKPRMSNTAFIVIHFADKVEYLSDGFLEKNRDTVYEEQINILKASKFPLVADLFRDDEDSVPATNTAKSRSSSKINVRSSRPLMKAPNKEHKKSVGYQFRTSLNLLMETLNATTPHYVRCIKPNDEKLPFHFDPKRAVQQLRACGVLETIRISAAGYPSRWTYHDFFNRYRVLMKKRELANTTDKKNICKSVLESLIKDPDKFQFGRTKIFFRAGQVAYLEKLRADKFREATIMIQKTVRGWLQRVKYRRLRAATLTLQRFCRGYLARRLTEHLRRTRAAIVFQKQYRMLKARRAYCRVRRAAVIIQSYTRGHVCTQKLPPVLTEHKATIIQKYARGWMARRHFQRQRDAAIVIQCAFRRLKARQALKALKIEARSAEHLKRLNVGMENKVVQLQRKIDDQNKEFKTLSEQLSAVTSTHAMEVEKLKKELARYQQNQEADPSLQLQEEVQSLRTELQKAHSERRVLEDAHNRENGELRKRVADLEHENALLKDEKEHLNHQILRQSKAESSQSSVEENLLIKKELEEERSRYQNLVKEYSQLEQRYENLRDEQQTPGHRKNPSNQSSLESDSNYPSISTSEIGDTEDALQQVEEIGIEKAAMDMTVFLKLQKRVRELEQERKKLQVQLEKEQQDSKKVQVEQQNNGLDVDQDADIAYNSLKRQELESENKKLKNDLNERWKAVADQAMQDNSTHSSPDSYSLLLNQLKLANEELEVRKEEVLILRTQIMNADQRRLSGKNMEPNINARTSWPNSEKHVDQEDAIEAYHGVCQTNSQTEDWGYLNEDGELGLAYQGLKQVARLLEAQLQAQNLKHEEEVEHLKAQVEAMKEEMDKQQQTFCQTLLLSPEAQVEFGVQQEISRLTNENLDFKELVEKLEKNEKKLKKQLKIYMKKVQDLEAAQALAQSDRRHHELTRQVTVQRKEKDFQGMLEYHKEDEALLIRNLVTDLKPQMLSGTVPCLPAYILYMCIRHADYTNDDLKVHSLLSSTINGIKKVLKKHNEDFEMTSFWLSNTCRLLHCLKQYSGDEGFMTQNTAKQNEHCLKNFDLTEYRQVLSDLSIQIYQQLIKIAEGLLQPMIVSAMLENESIQGLSGVRPTGYRKRSSSMVDGENSYCLEAIIRQMNFFHTVLCDQGLDPEIILQVFKQLFYMINAVTLNNLLLRKDACSWSTGMQLRYNISQLEEWLRGKNLQQSGAVQTMEPLIQAAQLLQLKKKTQEDAEAICSLCTSLSTQQIVKILNLYTPLNGFEERVTVSFIRTIQAQLQERSDPQQLLLDSKHMFPVLFPFNPSALTMDSIHIPACLNLEFLNEV AEC1B6F37389EE48 rfip2_human Rab11 family-interacting protein 2 NRip11 RAB11FIP2 KIAA0941 protein protein human Homo sapiens Human MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPGLLIQGSPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNIQFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKNDGTFSDTSSAIIPSTHMPDANSEFSSGEIQMKSKPKKPFLLGPQRLSSAHSMSDLSGSHMSSEKLKAGTIGQTHLLGHQLDSFGTVPESGSLKSPHRRTLSFDTSKMNQPDSIVDEGELCFGRQNDPFTNVTASLPQKFATLPRKKNPFEESSETWDSSMNLFSKPIEIRKENKREKREKVSLFERVTGKKDSRRSDKLNNGGSDSPCDLKSPNAFSENRQDYFDYESTNPFTAKFRASNIMPSSSFHMSPTSNEDLRKIPDSNPFDATAGYRSLTYEEVLQELVKHKELLRRKDTHIRELEDYIDNLLVRVMEETPSILRVPYEPSRKAGKFSNS DIP protein Q7L804 original sequence version: 30 9EC2DAB0A585BCD3 d3zd48_rat Rab11fip2 Rab11fip2_predicted rCG_57662 protein protein rat Rattus norvegicus (Rat) Rat MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPGLLMQGNPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNIQFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKSDGVFSDTSSAIVPSTHMPDANPEFSSGEMQMKSKPKKPFLLGPQRLSSAHSMSDLTGSHLSSEKLKSSTVGPTHLLSHQINSFGVVPESGSLKSPHRRTLSFDTSKLNQPGSIVDEGEHCFGRQSDPFTNVTASLPQKFATLPRKKNPFEESSEPWDSSTNLFSKPIEVRKESKREKREKVSLFERVTGKRDSRRSDKLNNGGSDSPCDLKSPSAFNENRQDYFEYESTNPFTAKFRASTIMPSSSFHMNPTSSDDLRKIPDSNPFDATAGYRSLTYEEVLQELVKHKELLRRKDTHIRELEDYIDNLLVRVMEETPSILRVPYEPSRKAGKFTNS AB9C9E6E89EDFF2E tfr1_rat Transferrin receptor protein 1 Tfrc Trfr protein protein rat Rattus norvegicus (Rat) Rat MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAADEEENADSNMKASVRKPKRFNGRLCFATIAVVIFFLIGFMIGYLGYCKRVEQKEECVRLAEAEEADKSENDETEYVPKSSRLFWADLKTLLSEKLNSIEFTDIIKQLSQNTYTPREAGSQKDENLAYYIENLFHDFKFSKVWRDEHYVKIQVKNSVSQNLVTINSGSNIDPVEAPEGYVAFSKAGEVTGKLVHANFGTKKDFEELNYSVNGSLVIVRAGKITFAEKVANAQSFNAIGVLIYMDRNTFPVVEADLQFFGHAHLGTGDPYTPGFPSFNHTQFPPSQSSGLPSIPVQTISRAAAEKLFKNMEGNCPPSWNIDSSCKLELSQNQNVKLTVNNVLKETRILNIFGVIKGYEEPDRYIVVGAQRDAWGPGVAKSSVGTGLLLKLAQVFSDMISKDGFRPSRSIIFASWTAGDYGAVGATEWLEGYLSSLHLKAFTYINLDKVVLGTSNFKVSASPLLYTLMGKIMQDVKHPIDGKYLYRDSNWISKIEELSLDNAAFPFLAYSGIPAVSFCFCEDEDYPYLGTKLDTYEILIQKVPQLNQMVRTAAEVAGQFIIKLTHDIELTLDYEMYNSKLLSFMKDLNQFKADIKDMGLSLQWLYSARGDYFRATSRLTTDFHNAEKTNRFVMREINDRIMKVEYHFLSPYVSPRESPFRHIFWGSGSHTLSALVENLRLRQKNITAFNETLFRNQLALATWTIQGVANALSGDIWNIDNEF 19488AD6D833249D rb11a_rat Ras-related protein Rab-11A Rab11a Rab11 24KG protein protein rat Rattus norvegicus (Rat) Rat MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI DIP protein P62494 original sequence version: 68 76FC1E113A29B269 dlg4-myo5b-1 1 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Cultured hippocampal neurons (DIV19) were fixed and immunolabeled for endogenous MyoVb and PSD-95 (left). Fig. 1B, Supp. Fig. 1C tfrc-myo5b-1 3 8 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization TfR-mCh labeled REs in spines colocalized with GFP-MyoVb clusters and both showed correlated movement into and out of spines (Figures 1C and 1E; Movies S1 and S2). Fig. 1C, 1E A C-terminal domain of MyoVb was shown to be required for the interaction, coordinates not given. myo5b-atp-1 4 6 enzyme target enzyme target substrate substrate neutral component neutral component 8 enzyme enzyme neutral component neutral component n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection atpase reaction atpase reaction Ca2+ The calculated Vmax = 1.17 s1motor-1 and and Kactin = 12.58 uM in the absence of Ca2+ and 12.07 s-1motor-1 and 7.11 uM in the presence of 100 uM Ca2+ Fig. 3A myo5b-rab11-1 2 10 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Coimmunoprecipitation (co-IP) revealed that MyoVb forms a complex with Rab11 and Rab11-FIP2 in brain (Figure 3E). Fig. 3E myo5b-rab11-2 2 10 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Coimmunoprecipitation (co-IP) revealed that MyoVb forms a complex with Rab11 and Rab11-FIP2 in brain (Figure 3E). Fig. 3E rab11fip2-myo5b-1 5 8 unspecified role unspecified role prey prey region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Co-IP was performed on lysates of 293T cells expressing V5-tagged MyoVb and GFP-tagged Rab11-FIP2 in different concentrations of free Ca2+. ms, control mouse serum. Fig. 3F, 3H Ca2+ A version of Myo5B locked in an open conformation due to deletion of the coiled coil region (949-1042) was able to bind RAB11FIP2 in the absence of Ca2+ suggesting that the Ca2+-dependent extension of MyoVb enables its association with cargo rab11fip2-myo5b-2 3 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization whereas MyoVb-WT (wild-type) showed only partial overlap with Rab11-FIP2 and TfR-positive REs, the constitutively extended MyoVb-CCtr mutant strongly colocalized with Rab11-FIP2 Figs. 4A, 4C, 5, Supp. Fig. 3F A C-terminal domain of MyoVb was shown to be required for the interaction, coordinates not given. Myo5B mutants lacking the coiled-coil region (949-1042) and locked in an open conformation demonstrated increased colocalisation Ca2+ Glycine tfrc-rab11fip2-1 3 11 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Colocalization of transferrin receptors (TfR) and Rab11-FIP2 in neuronal cell bodies Supp. Fig. 3C-F