IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ RACK1 and CIS mediate the degradation of BIMEL in cancer cells. RACK1 and CIS mediate the degradation of BIMEL in cancer cells. J. Biol. Chem. (0021-9258) 2008 Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer lu-hai.wang@mssm.edu 16-Dec-2008: Contacted by JYOTI. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu Accepted 2008-Not a month!-5 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2008 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. RACK1 and CIS mediate the degradation of BIMEL in cancer cells. J. Biol. Chem. (0021-9258) 2008 Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer lu-hai.wang@mssm.edu 16-Dec-2008: Contacted by JYOTI. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu Accepted 2008-Not a month!-5 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. Paclitaxel treatment or no treatment J. Biol. Chem. (0021-9258) 2008 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. RACK1 and CIS mediate the degradation of BIMEL in cancer cells. J. Biol. Chem. (0021-9258) 2008 Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer lu-hai.wang@mssm.edu 16-Dec-2008: Contacted by JYOTI. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu Accepted 2008-Not a month!-5 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2008 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. RACK1 and CIS mediate the degradation of BIMEL in cancer cells. J. Biol. Chem. (0021-9258) 2008 Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer lu-hai.wang@mssm.edu 16-Dec-2008: Contacted by JYOTI. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu Accepted 2008-Not a month!-5 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2008 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. RACK1 and CIS mediate the degradation of BIMEL in cancer cells. J. Biol. Chem. (0021-9258) 2008 Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer lu-hai.wang@mssm.edu 16-Dec-2008: Contacted by JYOTI. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu Accepted 2008-Not a month!-5 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2008 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. RACK1 and CIS mediate the degradation of BIMEL in cancer cells. J. Biol. Chem. (0021-9258) 2008 Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer lu-hai.wang@mssm.edu 16-Dec-2008: Contacted by JYOTI. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. lu-hai.wang@mssm.edu Accepted 2008-Not a month!-5 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2008 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. RACK1 and CIS mediate the degradation of BIMEL in cancer cells. J. Biol. Chem. (0021-9258) 2008 Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer lu-hai.wang@mssm.edu 16-Dec-2008: Contacted by JYOTI. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Coimmunoprecipitation was carried out upon the glycerol gradient fractions. lu-hai.wang@mssm.edu Accepted 2008-Not a month!-5 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2008 RACK1 and CIS mediate the degradation of BIMEL in cancer cells. RACK1 and CIS mediate the degradation of BIMEL in cancer cells. J. Biol. Chem. (0021-9258) 2008 Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer lu-hai.wang@mssm.edu 16-Dec-2008: Contacted by JYOTI. human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhang W., Cheng GZ., Gong J., Hermanto U., Zong CS., Chan J., Cheng JQ., Wang LH. Synapse - Interactions of proteins with an established role in the presynapse. Coimmunoprecipitation was carried out upon the glycerol gradient fractions. lu-hai.wang@mssm.edu Accepted 2008-Not a month!-5 by ORCHARD. 16-Dec-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2008 rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR DIP protein P63244 original sequence version: 71 257F91E369ED2044 o54918-1 Bcl-2-like protein 11 Bcl2l11 Bim Bcl2-interacting mediator of cell death protein protein mouse Mus musculus Mouse MAKQPSDVSSECDREGGQLQPAERPPQLRPGAPTSLQTEPQGNPDGEGDRCPHGSPQGPLAPPASPGPFATRSPLFIFVRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASIRQSQEEPEDLRPEIRIAQELRRIGDEFNETYTRRVFANDYREAEDHPQMVILQLLRFIFRLVWRRH 531C176E5F1AC9AA cish_human Cytokine-inducible SH2-containing protein CIS-1 Suppressor of cytokine signaling Protein G18 CISH G18 protein protein human Homo sapiens Human MVLCVQGPRPLLAVERTGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSRPRILAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPMPKEDAPSDPALPAPPPATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMADYLRQYPFQL 0BA97804E3B0F608 dyl1_human Dynein light chain 1, cytoplasmic DYNLL1 DLC1 DNCLC1 HDLC1 Dynein light chain LC8-type 1 8 kDa dynein light chain Protein inhibitor of neuronal nitric oxide synthase DNCL1 protein protein human Homo sapiens Human MCDRKAVIKNADMSEEMQQDSVECATQALEKYNIEKDIAAHIKKEFDKKYNPTWHCIVGRNFGSYVTHETKHFIYFYLGQVAILLFKSG DIP protein P63167 original sequence version: 108 F5E7647D092BEB3A tom20_human Mitochondrial import receptor subunit TOM20 homolog Outer mitochondrial membrane receptor Tom20 TOMM20 KIAA0016 Mitochondrial 20 kDa outer membrane protein protein protein human Homo sapiens Human MVGRNSAIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRIVSAQSLAEDDVE DIP protein Q15388 original sequence version: 139 5153BD25AC5D9B3A b2l11_human Bcl-2-like protein 11 BCL2L11 BIM Bcl2-interacting mediator of cell death protein protein human Homo sapiens Human MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSPQGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPRMVILRLLRYIVRLVWRMH DIP protein O43521 original sequence version: 48 DIP protein O43521-1 original sequence version: 71 DIP protein O43521 original sequence version: 132 DIP protein O43521 original sequence version: 143 D75735E469CA6997 o43521-1 Bcl-2-like protein 11 Bim(EL) BCL2L11 BIM Bcl2-interacting mediator of cell death protein protein human Homo sapiens Human MAKQPSDVSSECDREGRQLQPAERPPQLRPGAPTSLQTEPQGNPEGNHGGEGDSCPHGSPQGPLAPPASPGPFATRSPLFIFMRRSSLLSRSSSGYFSFDTDRSPAPMSCDKSTQTPSPPCQAFNHYLSAMASMRQAEPADMRPEIWIAQELRRIGDEFNAYYARRVFLNNYQAAEDHPRMVILRLLRYIVRLVWRMH D75735E469CA6997 dynll1-gnb2l1 6 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey physical association physical association Co-immunoprecipitation assay was carried out in HEK293T cells transiently transfected with Flag-DLC1 to confirm the yeast two-hybrid result. The interaction between RACK1 and DLC1 was detected upon treatment of the cells with UV-irradiation, paclitaxel, or staurosporine P63167 transfection 1 A UV-irradiation - This interaction occurred upon treatment of cells with UV-irradiation. Paclitaxel - This interaction occurred upon treatment of cells with paclitaxel. Staurosporine - This interaction occurred upon treatment of cells with staurosporine. bcl2l2l11-gnb2l1 5 14 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 1 B Paclitaxel - This interaction occurred upon treatment of cells with paclitaxel. Staurosporine - This interaction occurred upon treatment of cells with staurosporine. In MCF7 breast cancer cells, endogenous RACK1 was able to be co-immunoprecipitated with anti-Bim antibody, which was greatly enhanced upon paclitaxel or staurosporine treatment dynll1-bcl2l11 1 12 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association 1 C Paclitaxel - These interactions were increased by treating cell with paclitaxel. dynll1-gnb2l1-1 4 12 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region WD1-4 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range range range ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 D HIS-RACK1 and HIS-WD1-4 directly interacted with GST-DLC1 The range for the WD repeats 1-4 for GNB2L1 (RACK1) was taken from the UniProtKB entry for the protein. The peptide pWD4 based on aa 138-146 of GNB2L1 (RACK1) (CKYTVQDES) inhibits this interaction. bcl2l11-gnb2l1 4 9 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 1 E RACK1 also interacted with BimEL directly in vitro This interaction was enhanced by the presence DYNLL1 (DLC1). bcl2l11-gnb2l1-1 3 10 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region WD1-4 sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association S1 O54918-1; P63244 transfection The interaction between RACK1 and BimEL was also through N-terminal portion of RACK1 The range for the WD repeats 1-4 was taken from the UniProtKB entry for GNB2L1 (RACK1), P63244. Paclitaxel treatment of cells decreased this interaction. bcl2l11-dynll1 2 15 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component physical association physical association S2 A BimEL distributed along with DLC1 and -tubulin No paclitaxel treatment bcl2l11-dynll1-1 2 9 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component physical association physical association S2 A Paclitaxel treatment both DLC1 and BimEL shifted from the heavier to the lighter fractions 16-20 (peaked at 18-19 and 18-20 for BimEL and DLC1, respectively), and corresponded to the sedimentation positions of mitochondrial outer membrane protein, Tom20 Paclitaxel induced release of BCL2L11 (BimEL) and DYNLL1 (DLC1) from microtubule. bcl2l11-gnb2l1-2 8 14 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association S2 B Little interaction between RACK1 and BimEL This interaction was enhanced when cells were treated with paclitaxel. dynll1-gnb2l1-2 7 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association S2 C between RACK1 and DLC1 Paclitaxel treatment of cells enhanced this interaction.