IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Mamm. Genome (0938-8990) 2007 imex curation Peterson EA., Kalikin LM., Steels JD., Estey MP., Trimble WS., Petty EM. Synapse - Interactions of proteins with an established role in the presynapse. epetty@umich.edu 14-Nov-2008: Contacted by JYOTI. Human testis cDNA davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Peterson EA., Kalikin LM., Steels JD., Estey MP., Trimble WS., Petty EM. Synapse - Interactions of proteins with an established role in the presynapse. SEPT14 also interacts with SEPT5 but origin of construct could not be traced. epetty@umich.edu Accepted 2008-DEC-5 by DAVET. 14-Nov-2008: Contacted by JYOTI. Human testis cDNA Mamm. Genome (0938-8990) 2007 davet Only protein-protein interactions imex curation Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Mamm. Genome (0938-8990) 2007 imex curation Peterson EA., Kalikin LM., Steels JD., Estey MP., Trimble WS., Petty EM. Synapse - Interactions of proteins with an established role in the presynapse. epetty@umich.edu 14-Nov-2008: Contacted by JYOTI. Human testis cDNA davet Only protein-protein interactions rat-testis Rat testis testis anti bait coip anti bait coimmunoprecipitation anti bait coip fingerprinting peptide massfingerprinting fingerprinting Peterson EA., Kalikin LM., Steels JD., Estey MP., Trimble WS., Petty EM. Synapse - Interactions of proteins with an established role in the presynapse. epetty@umich.edu Accepted 2008-DEC-5 by DAVET. 14-Nov-2008: Contacted by JYOTI. Human testis cDNA Mamm. Genome (0938-8990) 2007 davet Only protein-protein interactions imex curation Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Mamm. Genome (0938-8990) 2007 imex curation Peterson EA., Kalikin LM., Steels JD., Estey MP., Trimble WS., Petty EM. Synapse - Interactions of proteins with an established role in the presynapse. epetty@umich.edu 14-Nov-2008: Contacted by JYOTI. Human testis cDNA davet Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. confocal microscopy confocal microscopy immunostaining immunostaining Peterson EA., Kalikin LM., Steels JD., Estey MP., Trimble WS., Petty EM. Synapse - Interactions of proteins with an established role in the presynapse. epetty@umich.edu Accepted 2008-DEC-5 by DAVET. 14-Nov-2008: Contacted by JYOTI. Human testis cDNA Mamm. Genome (0938-8990) 2007 davet Only protein-protein interactions imex curation Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Mamm. Genome (0938-8990) 2007 imex curation Peterson EA., Kalikin LM., Steels JD., Estey MP., Trimble WS., Petty EM. Synapse - Interactions of proteins with an established role in the presynapse. epetty@umich.edu 14-Nov-2008: Contacted by JYOTI. Human testis cDNA davet Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Peterson EA., Kalikin LM., Steels JD., Estey MP., Trimble WS., Petty EM. Synapse - Interactions of proteins with an established role in the presynapse. epetty@umich.edu Accepted 2008-DEC-5 by DAVET. 14-Nov-2008: Contacted by JYOTI. Human testis cDNA Mamm. Genome (0938-8990) 2007 davet Only protein-protein interactions imex curation Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Characterization of a SEPT9 interacting protein, SEPT14, a novel testis-specific septin. Mamm. Genome (0938-8990) 2007 imex curation Peterson EA., Kalikin LM., Steels JD., Estey MP., Trimble WS., Petty EM. Synapse - Interactions of proteins with an established role in the presynapse. epetty@umich.edu 14-Nov-2008: Contacted by JYOTI. Human testis cDNA davet Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Peterson EA., Kalikin LM., Steels JD., Estey MP., Trimble WS., Petty EM. Synapse - Interactions of proteins with an established role in the presynapse. epetty@umich.edu Accepted 2008-DEC-5 by DAVET. Human testis cDNA Mamm. Genome (0938-8990) 2007 davet Only protein-protein interactions imex curation sept7_rat Septin-7 Septin7 CDC10 protein homolog Cdc10 Sept7 protein protein rat Rattus norvegicus (Rat) Rat MSVSARSAAAEERSVNCSTMAQPKNLEGYVGFANLPNQVYRKSVKRGFEFTLMVVGESGLGKSTLINSLFLTDLYSPEYPGPSHRIKKTVQVEQSKVLIKEGGVQLLLTIVDTPGFGDAVDNSNCWQPVIDYIDSKFEDYLNAESRVNRRQMPDNRVQCCLYFIAPSGHGLKPLDIEFMKRLHEKVNIIPLIAKADTLTPEECQQFKKQIMKEIQGHKIKIYEFPETDDEEENKLVKKIKDRLPLAVVGSNTIIEVNGKRVRGRQYPWGVAEVENGEHCDFTILRNMLIRTHMQDLKDVTNNVHYENYRSRKLAAVTYNGVDNNKNKGQLTKSPLAQMEEERREHVAKMKKMEMEMEQVFEMKVKEKVQKLKDSEAELQRRHEQMKKNLEAQHKELEEKRRQFEEEKANWEAQQRILEQQNSSRTLEKNKKKGKIF 666DC3C1F0880404 sept2_rat Septin-2 Septin2 Sept2 Vascular endothelial cell specific protein 11 Vesp11 protein protein rat Rattus norvegicus (Rat) Rat MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERIIPGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINSRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHSIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDTDSSTLGHHV C4B9335F18098641 q9uhd8-1 Septin-9 SEPTIN9 KIAA0991 MLL septin-like fusion protein MSF-A MSF Ovarian/Breast septin SEPT9 Septin D1 Epsilon MSF-A protein protein human Homo sapiens Human MKKSYSGGTRTSSGRLRRLGDSSGPALKRSFEVEEVETPNSTPPRRVQTPLLRATVASSTQKFQDLGVKNSEPSARHVDSLSQRSPKASLRRVELSGPKAAEPVSRRTELSIDISSKQVENAGAIGPSRFGLKRAEVLGHKTPEPAPRRTEITIVKPQESAHRRMEPPASKVPEVPTAPATDAAPKRVEIQMPKPAEAPTAPSPAQTLENSEPAPVSQLQSRLEPKPQPPVAEATPRSQEATEAAPSCVGDMADTPRDAGLKQAPASRNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGMEEKEPEAPEM D4404578328CFCFE q9uhd8-2 Septin-9 SEPTIN9 KIAA0991 MLL septin-like fusion protein MSF-A MSF Ovarian/Breast septin SEPT9 Septin D1 Alpha protein protein human Homo sapiens Human MERDRISALKRSFEVEEVETPNSTPPRRVQTPLLRATVASSTQKFQDLGVKNSEPSARHVDSLSQRSPKASLRRVELSGPKAAEPVSRRTELSIDISSKQVENAGAIGPSRFGLKRAEVLGHKTPEPAPRRTEITIVKPQESAHRRMEPPASKVPEVPTAPATDAAPKRVEIQMPKPAEAPTAPSPAQTLENSEPAPVSQLQSRLEPKPQPPVAEATPRSQEATEAAPSCVGDMADTPRDAGLKQAPASRNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGMEEKEPEAPEM 47A03DD79B5D6147 sept9_rat Septin-9 Septin9 Eighth septin Eseptin Sept9 Septin-like protein protein protein rat Rattus norvegicus (Rat) Rat MERDRITALKRSFEVEEIEPPNSTPPRRVQTPLLRATVASSSQKFQDLGVKNSEPAARLVDTLSQRSPKPSLRRVDLAGAKAPEPMSRRTELSIDISSKQVESTASTPGPSRFGLKRAEVLGHKTPEPVPRRTEITIVKPQESGLRRVETPASKAPEGSAMPVTDAAPKRVEIQVPKPAEAPNCPLPPQTLENSEAPMSQLQSRLEPRPPVTEVPYRNQEDSEVAPSCVVDMADNPRDAMLKQAPVSRNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPISEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVYFKQRITSDLLSNGIDVYPQKEFDEAEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSNIHFEAYRVKRLNEGNSAMANGIEKEPETQEM B45160157F986C0C sep14_human Septin-14 SEPTIN14 SEPT14 protein protein human Homo sapiens Human MAERTMAMPTQIPADGDTQKENNIRCLTTIGHFGFECLPNQLVSRSIRQGFTFNILCVGETGIGKSTLIDTLFNTNLKDNKSSHFYSNVGLQIQTYELQESNVQLKLTVVETVGYGDQIDKEASYQPIVDYIDAQFEAYLQEELKIKRSLFEYHDSRVHVCLYFISPTGHSLKSLDLLTMKNLDSKVNIIPLIAKADTISKNDLQTFKNKIMSELISNGIQIYQLPTDEETAAQANSSVSGLLPFAVVGSTDEVKVGKRMVRGRHYPWGVLQVENENHCDFVKLRDMLLCTNMENLKEKTHTQHYECYRYQKLQKMGFTDVGPNNQPVSFQEIFEAKRQEFYDQCQREEEELKQRFMQRVKEKEATFKEAEKELQDKFEHLKMIQQEEIRKLEEEKKQLEGEIIDFYKMKAASEALQTQLSTDTKKDKHRKK AFFF9A953F1FC637 sept2_human Septin-2 SEPTIN2 DIFF6 KIAA0158 NEDD5 Neural precursor cell expressed developmentally down-regulated protein 5 SEPT2 protein protein human Homo sapiens Human MSKQQPTQFINPETPGYVGFANLPNQVHRKSVKKGFEFTLMVVGESGLGKSTLINSLFLTDLYPERVIPGAAEKIERTVQIEASTVEIEERGVKLRLTVVDTPGYGDAINCRDCFKTIISYIDEQFERYLHDESGLNRRHIIDNRVHCCFYFISPFGHGLKPLDVAFMKAIHNKVNIVPVIAKADTLTLKERERLKKRILDEIEEHNIKIYHLPDAESDEDEDFKEQTRLLKASIPFSVVGSNQLIEAKGKKVRGRLYPWGVVEVENPEHNDFLKLRTMLITHMQDLQEVTQDLHYENFRSERLKRGGRKVENEDMNKDQILLEKEAELRRMQEMIARMQAQMQMQMQGGDGDGGALGHHV 12CCBBE30806F92F 12CCBBE30806F92F sept3_rat Neuronal-specific septin-3 Septin3 G-septin P40 Sept3 protein protein rat Rattus norvegicus (Rat) Rat MSKGLPEARTDTAMSELVPEPRPKPAVPMKPVSINSNLLGCIGIDTIIEQMRKKTMKTGFDFNIMVVGQSGLGKSTLVNTLFKSQVSRKASSWNREEKIPKTVEIKAIGHVIEEGGVKMKLTVIDTPGFGDQINNENCWEPIEKYINEQYEKFLKEEVNIARKKRIPDTRVHCCLYFISPTGHSLRPLDLEFMKHLSKVVNVIPVIAKADTMTLEEKSEFKQRVRKELEVNGIEFYPQKEFDEDLEDKTENDKIRQESMPFAVVGSDKEYQVNGKRVLGRKTPWGIIEVENLNHCEFALLRDFVIRTHLQDLKEVTHNIHYETYRAKRLNDNGGLPPGEGLLGTVLPPVPATPCPTAE 96F05B2CFD195599 sept4_human Septin-4 SEPTIN4 ARTS Apoptosis-related protein in the TGF-beta signaling pathway Bradeion beta Brain protein H5 CE5B3 beta Cell division control-related protein 2 Cerebral protein 7 PNUTL2 Peanut-like protein 2 SEP4 SEPT4 hucep-7 C17orf47 protein protein human Homo sapiens Human MDRSLGWQGNSVPEDRTEAGIKRFLEDTTDDGELSKFVKDFSGNASCHPPEAKTWASRPQVPEPRPQAPDLYDDDLEFRPPSRPQSSDNQQYFCAPAPLSPSARPRSPWGKLDPYDSSEDDKEYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVNSLFLTDLYRDRKLLGAEERIMQTVEITKHAVDIEEKGVRLRLTIVDTPGFGDAVNNTECWKPVAEYIDQQFEQYFRDESGLNRKNIQDNRVHCCLYFISPFGHGLRPLDVEFMKALHQRVNIVPILAKADTLTPPEVDHKKRKIREEIEHFGIKIYQFPDCDSDEDEDFKLQDQALKESIPFAVIGSNTVVEARGRRVRGRLYPWGIVEVENPGHCDFVKLRTMLVRTHMQDLKDVTRETHYENYRAQCIQSMTRLVVKERNRNKLTRESGTDFPIPAVPPGTDPETEKLIREKDEELRRMQEMLHKIQKQMKENY 2F08D3611EF6523D q5fvk1_rat RGD1305464 protein protein rat Rattus norvegicus (Rat) Rat MAGGFDTEARALSPSEPTVTRDEPESQVLAGKLPAPKAKKPGKKPPTPGPEKTETTTGEGSCNPSPSSGASTSPPGPTLRARFRNLLETAWLNGVALPTWGHKASGADRPSPHPQLLGSQTHHL 631D326A50B90F86 sep12_rat Septin-12 Septin12 Sept12 protein protein rat Rattus norvegicus (Rat) Rat MDERRTPSPCSSRPSSPRTPPCEMFGPVGIEAVLDQLRIKAMKTGFEFNIMVVGQSGLGKSTMVNTLFKSKVWQSPAPNLDVPMPQTLELHSVTHVIEEKGLKLKLTVTDTPGFGDQINNDKCWDPILSYINQQYEQYLQEELLITRQRHIPDTRVHCCVYFVPPTGHCLRPLDIEFLRRLCRTVNVVPVIARADSLTIEERDAFRSRIQQNLKNHCIDVYPQQCFDEDINDRLLNSKIREQIPFAVVGADREHIVNGRCVLGRKTKWGIIEVENMAHCEFLLLRDLLIRSHLQDLKDITHNVHYENYRVLRLNESHVLPRGPGWVNLAPASPGQLMAPGPEKVRKRSKDPRDDEC 598F80BE45A1F451 q9uhd8-3 Septin-9 SEPTIN9 KIAA0991 MLL septin-like fusion protein MSF-A MSF Ovarian/Breast septin SEPT9 Septin D1 Beta MSF-B protein protein human Homo sapiens Human MEPPASKVPEVPTAPATDAAPKRVEIQMPKPAEAPTAPSPAQTLENSEPAPVSQLQSRLEPKPQPPVAEATPRSQEATEAAPSCVGDMADTPRDAGLKQAPASRNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGMEEKEPEAPEM Contains a N-acetylmethionine at position 1. F581FB2EA1A6E063 sept7_human Septin-7 SEPTIN7 CDC10 CDC10 protein homolog SEPT7 protein protein human Homo sapiens Human MSVSARSAAAEERSVNSSTMVAQQKNLEGYVGFANLPNQVYRKSVKRGFEFTLMVVGESGLGKSTLINSLFLTDLYSPEYPGPSHRIKKTVQVEQSKVLIKEGGVQLLLTIVDTPGFGDAVDNSNCWQPVIDYIDSKFEDYLNAESRVNRRQMPDNRVQCCLYFIAPSGHGLKPLDIEFMKRLHEKVNIIPLIAKADTLTPEECQQFKKQIMKEIQEHKIKIYEFPETDDEEENKLVKKIKDRLPLAVVGSNTIIEVNGKRVRGRQYPWGVAEVENGEHCDFTILRNMLIRTHMQDLKDVTNNVHYENYRSRKLAAVTYNGVDNNKNKGQLTKSPLAQMEEERREHVAKMKKMEMEMEQVFEMKVKEKVQKLKDSEAELQRRHEQMKKNLEAQHKELEEKRRQFEDEKANWEAQQRILEQQNSSRTLEKNKKKGKIF DIP protein Q16181 original sequence version: 122 DIP protein Q16181 original sequence version: 130 946A08C54B7124FF sep11_rat Septin-11 Septin11 Sept11 protein protein rat Rattus norvegicus (Rat) Rat MAVAVGRPSNEELRNLSLSGHVGFDSLPDQLVNKSTSQGFCFNILCVGETGIGKSTLMDTLFNTKFESDPATHNEPGVRLKARSYELQESNVRLKLTIVDTVGFGDQINKDDSYKPIVEYIDAQFEAYLQEELKIKRSLFNYHDTRIHACLYFIAPTGHSLKSLDLVTMKKLDSKVNIIPIIAKADTIAKNELHKFKSKIMSELVSNGVQIYQFPTDEETVAEINATMSVHLPFAVVGSTEEVKIGNKMAKARQYPWGVVQVENENHCDFVKLREMLIRVNMEDLREQTHTRHYELYRRCKLEEMGFKDTDPDSKPFSLQETYEAKRNEFLGELQKKEEEMRQMFVMRVKEKEAELKEAEKELHEKFDLLKRTHQEEKKKVEDKKKELEEEVSNFQKKKAAAQLLQSQAQQSGAQQTKKDKDKKNPWLCTE CC838B791729B511 sept1_human Septin-1 SEPTIN1 DIFF6 LARP PNUTL3 Peanut-like protein 3 SEPT1 Serologically defined breast cancer antigen NY-BR-24 protein protein human Homo sapiens Human MAGGVMDKEYVGFAALPNQLHRKSVKKGFDFTLMVAGESGLGKSTLINSLFLTNLYEDRQVPEASARLTQTLAIERRGVEIEEGGVKVKLTLVDTPGFGDSVDCSDCWLPVVKFIEEQFEQYLRDESGLNRKNIQDSRVHCCLYFISPFGRGLRPLDVAFLRAVHEKVNIIPVIGKADALMPQETQALKQKIRDQLKEEEIHIYQFPECDSDEDEDFKRQDAEMKESIPFAVVGSCEVVRDGGNRPVRGRRYSWGTVEVENPHHCDFLNLRRMLVQTHLQDLKEVTHDLLYEGYRARCLQSLARPGARDRASRSKLSRQSATEIPLPMLPLADTEKLIREKDEELRRMQEMLEKMQAQMQQSQAQGEQSDAL 00F2752A819E1F16 sept14-sept9-1 5 8 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Here we present the identification of SEPT14 by yeast two-hybrid as a novel septin family member and interacting protein of SEPT9 The sequence given in UniProtKB for Q6ZU15 extends the sequence at the C-terminal by an additional 25 amino acid residues to that given in Fig. 2 of this paper. sept14-sept9-2 1 11 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Here we present the identification of SEPT14 by yeast two-hybrid as a novel septin family member and interacting protein of SEPT9 Fig. 1 sept14-sept9-3 1 11 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait physical association physical association Here we present the identification of SEPT14 by yeast two-hybrid as a novel septin family member and interacting protein of SEPT9 Fig. 1 sept14-sept9-4 1 9 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association Here we present the identification of SEPT14 by yeast two-hybrid as a novel septin family member and interacting protein of SEPT9 Fig. 1 sept14-sept1-1 1 11 unspecified role unspecified role bait bait 20 unspecified role unspecified role prey prey physical association physical association Fig. 4 sept14-sept2-1 1 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig. 4 sept14-sept3-1 1 13 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig. 4 sept14-sept4-1 1 11 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association Fig. 4 sept14-sept7-1 1 18 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig. 4 sept14-sept11-1 1 11 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey physical association physical association Fig. 4 sept14-sept12-1 1 11 unspecified role unspecified role bait bait 16 unspecified role unspecified role prey prey physical association physical association Fig. 4 sept14-sept9-5 1 8 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Fig. 4 sept14-sept9-6 3 11 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Overexpressed Flag-SEPT9 and GFP-SEPT14 colocalize to stress fibers in CHO cells Fig. 6 sept14-sept9-7 4 17 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Coimmunoprecipitation of SEPT9 and SEPT14 Fig. 7 sept9-1 2 10 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association The lower unique band (asterisk) contained several peptides specific for rat SEPT2, while the upper band (<) contained peptides for rat SEPT7, SEPT9, and SEPT14 Fig. 8