IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. Cell (0092-8674) 2007 imex curation Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. jzchuang@med.cornell.edu,chsung@med.cornell.edu 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). davet Only protein-protein interactions 7 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. Source of STX3 inferred from previous work of contributer jzchuang@med.cornell.edu,chsung@med.cornell.edu izchuang@med.cornell.edu Accepted 2007-OCT-21 by DAVET. 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). Cell (0092-8674) 2007 davet Only protein-protein interactions 7 imex curation SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. Cell (0092-8674) 2007 imex curation Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. jzchuang@med.cornell.edu,chsung@med.cornell.edu 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). davet Only protein-protein interactions 7 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. jzchuang@med.cornell.edu,chsung@med.cornell.edu izchuang@med.cornell.edu Accepted 2007-OCT-21 by DAVET. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). Cell (0092-8674) 2007 davet Only protein-protein interactions 7 imex curation SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. Cell (0092-8674) 2007 imex curation Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. jzchuang@med.cornell.edu,chsung@med.cornell.edu 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). davet Only protein-protein interactions 7 human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy immunostaining immunostaining Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. Source of Stx-3 inferred from previous work of contributer jzchuang@med.cornell.edu,chsung@med.cornell.edu izchuang@med.cornell.edu Accepted 2007-OCT-21 by DAVET. 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). Cell (0092-8674) 2007 davet Only protein-protein interactions 7 imex curation SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. Cell (0092-8674) 2007 imex curation Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. jzchuang@med.cornell.edu,chsung@med.cornell.edu 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). davet Only protein-protein interactions 7 in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. jzchuang@med.cornell.edu,chsung@med.cornell.edu izchuang@med.cornell.edu Accepted 2007-OCT-21 by DAVET. 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). Cell (0092-8674) 2007 davet Only protein-protein interactions 7 imex curation SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. Cell (0092-8674) 2007 imex curation Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. jzchuang@med.cornell.edu,chsung@med.cornell.edu 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). davet Only protein-protein interactions 7 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. Source of STX3 inferred from previous work of contributer jzchuang@med.cornell.edu,chsung@med.cornell.edu izchuang@med.cornell.edu Accepted 2007-OCT-21 by DAVET. 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). Cell (0092-8674) 2007 davet Only protein-protein interactions 7 imex curation SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. SARA-regulated vesicular targeting underlies formation of the light-sensing organelle in mammalian rods. Cell (0092-8674) 2007 imex curation Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. jzchuang@med.cornell.edu,chsung@med.cornell.edu 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). davet Only protein-protein interactions 7 mouse-retina Mus musculus retina retina retinal tissue anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Chuang JZ., Zhao Y., Sung CH. Synapse - Interactions of proteins with an established role in the presynapse. jzchuang@med.cornell.edu,chsung@med.cornell.edu izchuang@med.cornell.edu Accepted 2007-OCT-21 by DAVET. 12-Oct-2007: Contacted by JYOTI. Bovine cDNA (no detail given in paper, inferred from species of interacting molecule). Cell (0092-8674) 2007 davet Only protein-protein interactions 7 imex curation f1mky4_bovin Zinc finger FYVE domain-containing protein ZFYVE9 protein protein bovin Bos taurus (Bovine) Bovine MENYFQAEAYNLDKVLDEFEQNEDETVSPILLDTKWNKILDPSSRQLSFNSALTSVNEPTVSESQPQLKVFSLAHSAPPDSEGEDHCANGQDCSLNPEINTMWIDENAVTEDQLIKRNCSRDDQCSTVEVGEKKCGNLACLPDEKNVLVVAVMHNCDKRTFQSDLQDCNNYNSQSLKNAFSCSLDNENRQTDQFSFSINGSTEKDMNSEKQMDPLNRPNAEGDSDKYICTTSDNLASVCSPSQLQDDENIDRDPSMSVITSLTLDSVISSQRTEEGPDVKQEDSTPDEVLTGKTSSPRTDLGSPNSFSHLSEEILMKKEPSEERTTEESTQSALPLLLKTDMPNGSGRDDNSEQVSDCLVPSEGKADDKEGCKHEEILGTKEFLNMTEHFSESQEMTNWKLTKPNKMNDSQVKKDNEKFLQMNQPEDTCDDSGECDRMAEAGLDLKGTCVNESEGCDFSTVMDTPAANSLSNCCDSYGMQSPVVCFVPKTLPSKEDSVTEEKEIEESKSECYSNIYEQRGNEATEGSGLLLNSTGDLTKKNYLHNFCSQVPSVLGQSSPKIVANLQSISVPFGGARPKQPSNLKLQIPKPLSDHLQNDLPANSGNTAKNKNDVLGKAKLGENSATNVCNASSGNISIADTNGDHLESYESGISSRPCLALAPESPDNDLRAGQFGISARKPFTTLGEVAPVWVPDSQAPNCMKCEARFTFTKRRHHCRACGKVFCASCCSLKCKLLYMDRKEARVCVICHSVLMNAQAWENMMSASSQSPNPNNPAEYCSTIPPLQQAQASGALSSPPPTVMVPVGVLKHPGAEVAQPREQRRVWFADGILPNGEVADAAKLTMNGTSSAGTLAVAHDPVKPAATSPLPAETNISLFSGSITQVGSPVGSAMNLIPEDGLPPILISTGVKGDYAVEEKPSQISVMQQLEDGGPDPLVFVLNANLLSMVKIVNYVNRKCWCFTTKGMHAVGQSEIVILLQCLPDEKCLPKDIFNHFVQLYRDALAGNVVGNLGHSFFSQSFLGSKEHGGFLYVTSTYQSLQDLVLPTPPYLFGILIQKWETPWAKVFPIRLMLRLGAEYRLYPCPLFSVRFRKPLFGETGHTIMNLLADFRNYQYTLPVVQGLVVDMEVRKTSIKIPSNRYNEMIKAMNKSNEHVLAGGACFNEKADSHLVCVQNDDGNYQTQAISIHNQPRKVTGASFFVFSGALKSSSGYLAKSSIVEDGVMVQITAENMDALRQALREMKDFTITCGKAEAEDPQEQIHIQWVDDDKDVNKGVLSPIDGKSMESITSVKIFHGSEYKANGKVIRWTEVFFLENDDQHNCLSDPADHSRLTEHVAKAFCLALCPHLKLLKEDGMTKLGLRVTLDSDQVGYQAGSNGQPLPSQYMNDLDSALVPVIHGGACQLSEGPIVMELIFYILENIA Homologous to O95405 5E336223EF0B3FB1 opsd_human Rhodopsin RHO OPN2 Opsin-2 protein protein human Homo sapiens Human MNGTEGPNFYVPFSNATGVVRSPFEYPQYYLAEPWQFSMLAAYMFLLIVLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVLGGFTSTLYTSLHGYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVMALACAAPPLAGWSRYIPEGLQCSCGIDYYTLKPEVNNESFVIYMFVVHFTIPMIIIFFCYGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWVPYASVAFYIFTHQGSNFGPIFMTIPAFFAKSAAIYNPVIYIMMNKQFRNCMLTTICCGKNPLGDDEASATVSKTETSQVAPA 6F4F6FCBA34265B2 pi3p 1-phosphatidyl-1D-myo-inositol 3-phosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) zfyv9_human Zinc finger FYVE domain-containing protein 9 ZFYVE9 MADHIP SARA SMADIP Mothers against decapentaplegic homolog-interacting protein Smad anchor for receptor activation Receptor activation anchor Novel serine protease protein protein human Homo sapiens Human MENYFQAEAYNLDKVLDEFEQNEDETVSSTLLDTKWNKILDPPSHRLSFNPTLASVNESAVSNESQPQLKVFSLAHSAPLTTEEEDHCANGQDCNLNPEIATMWIDENAVAEDQLIKRNYSWDDQCSAVEVGEKKCGNLACLPDEKNVLVVAVMHNCDKRTLQNDLQDCNNYNSQSLMDAFSCSLDNENRQTDQFSFSINESTEKDMNSEKQMDPLNRPKTEGRSVNHLCPTSSDSLASVCSPSQLKDDGSIGRDPSMSAITSLTVDSVISSQGTDGCPAVKKQENYIPDEDLTGKISSPRTDLGSPNSFSHMSEGILMKKEPAEESTTEESLRSGLPLLLKPDMPNGSGRNNDCERCSDCLVPNEVRADENEGYEHEETLGTTEFLNMTEHFSESQDMTNWKLTKLNEMNDSQVNEEKEKFLQISQPEDTNGDSGGQCVGLADAGLDLKGTCISESEECDFSTVIDTPAANYLSNGCDSYGMQDPGVSFVPKTLPSKEDSVTEEKEIEESKSECYSNIYEQRGNEATEGSGLLLNSTGDLMKKNYLHNFCSQVPSVLGQSSPKVVASLPSISVPFGGARPKQPSNLKLQIPKPLSDHLQNDFPANSGNNTKNKNDILGKAKLGENSATNVCSPSLGNISNVDTNGEHLESYEAEISTRPCLALAPDSPDNDLRAGQFGISARKPFTTLGEVAPVWVPDSQAPNCMKCEARFTFTKRRHHCRACGKVFCASCCSLKCKLLYMDRKEARVCVICHSVLMNAQAWENMMSASSQSPNPNNPAEYCSTIPPLQQAQASGALSSPPPTVMVPVGVLKHPGAEVAQPREQRRVWFADGILPNGEVADAAKLTMNGTSSAGTLAVSHDPVKPVTTSPLPAETDICLFSGSITQVGSPVGSAMNLIPEDGLPPILISTGVKGDYAVEEKPSQISVMQQLEDGGPDPLVFVLNANLLSMVKIVNYVNRKCWCFTTKGMHAVGQSEIVILLQCLPDEKCLPKDIFNHFVQLYRDALAGNVVSNLGHSFFSQSFLGSKEHGGFLYVTSTYQSLQDLVLPTPPYLFGILIQKWETPWAKVFPIRLMLRLGAEYRLYPCPLFSVRFRKPLFGETGHTIMNLLADFRNYQYTLPVVQGLVVDMEVRKTSIKIPSNRYNEMMKAMNKSNEHVLAGGACFNEKADSHLVCVQNDDGNYQTQAISIHNQPRKVTGASFFVFSGALKSSSGYLAKSSIVEDGVMVQITAENMDSLRQALREMKDFTITCGKADAEEPQEHIHIQWVDDDKNVSKGVVSPIDGKSMETITNVKIFHGSEYKANGKVIRWTEVFFLENDDQHNCLSDPADHSRLTEHVAKAFCLALCPHLKLLKEDGMTKLGLRVTLDSDQVGYQAGSNGQPLPSQYMNDLDSALVPVIHGGACQLSEGPVVMELIFYILENIV ADE5CB530C1272C6 eea1_human Early endosome antigen 1 EEA1 ZFYVE2 Endosome-associated protein p162 Zinc finger FYVE domain-containing protein 2 protein protein human Homo sapiens Human MLRRILQRTPGRVGSQGSDLDSSATPINTVDVNNESSSEGFICPQCMKSLGSADELFKHYEAVHDAGNDSGHGGESNLALKRDDVTLLRQEVQDLQASLKEEKWYSEELKKELEKYQGLQQQEAKPDGLVTDSSAELQSLEQQLEEAQTENFNIKQMKDLFEQKAAQLATEIADIKSKYDEERSLREAAEQKVTRLTEELNKEATVIQDLKTELLQRPGIEDVAVLKKELVQVQTLMDNMTLERERESEKLKDECKKLQSQYASSEATISQLRSELAKGPQEVAVYVQELQKLKSSVNELTQKNQTLTENLLKKEQDYTKLEEKHNEESVSKKNIQATLHQKDLDCQQLQSRLSASETSLHRIHVELSEKGEATQKLKEELSEVETKYQHLKAEFKQLQQQREEKEQHGLQLQSEINQLHSKLLETERQLGEAHGRLKEQRQLSSEKLMDKEQQVADLQLKLSRLEEQLKEKVTNSTELQHQLDKTKQQHQEQQALQQSTTAKLREAQNDLEQVLRQIGDKDQKIQNLEALLQKSKENISLLEKEREDLYAKIQAGEGETAVLNQLQEKNHTLQEQVTQLTEKLKNQSESHKQAQENLHDQVQEQKAHLRAAQDRVLSLETSVNELNSQLNESKEKVSQLDIQIKAKTELLLSAEAAKTAQRADLQNHLDTAQNALQDKQQELNKITTQLDQVTAKLQDKQEHCSQLESHLKEYKEKYLSLEQKTEELEGQIKKLEADSLEVKASKEQALQDLQQQRQLNTDLELRATELSKQLEMEKEIVSSTRLDLQKKSEALESIKQKLTKQEEEKKILKQDFETLSQETKIQHEELNNRIQTTVTELQKVKMEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKHQLQVQMENTLKEQKELKKSLEKEKEASHQLKLELNSMQEQLIQAQNTLKQNEKEEQQLQGNINELKQSSEQKKKQIEALQGELKIAVLQKTELENKLQQQLTQAAQELAAEKEKISVLQNNYEKSQETFKQLQSDFYGRESELLATRQDLKSVEEKLSLAQEDLISNRNQIGNQNKLIQELKTAKATLEQDSAKKEQQLQERCKALQDIQKEKSLKEKELVNEKSKLAEIEEIKCRQEKEITKLNEELKSHKLESIKEITNLKDAKQLLIQQKLELQGKADSLKAAVEQEKRNQQILKDQVKKEEEELKKEFIEKEAKLHSEIKEKEVGMKKHEENEAKLTMQITALNENLGTVKKEWQSSQRRVSELEKQTDDLRGEIAVLEATVQNNQDERRALLERCLKGEGEIEKLQTKVLELQRKLDNTTAAVQELGRENQSLQIKHTQALNRKWAEDNEVQNCMACGKGFSVTVRRHHCRQCGNIFCAECSAKNALTPSSKKPVRVCDACFNDLQG 51BA418F561E3411 stx3_human Syntaxin-3 STX3 STX3A protein protein human Homo sapiens Human MKDRLEQLKAKQLTQDDDTDAVEIAIDNTAFMDEFFSEIEETRLNIDKISEHVEEAKKLYSIILSAPIPEPKTKDDLEQLTTEIKKRANNVRNKLKSMEKHIEEDEVRSSADLRIRKSQHSVLSRKFVEVMTKYNEAQVDFRERSKGRIQRQLEITGKKTTDEELEEMLESGNPAIFTSGIIDSQISKQALSEIEGRHKDIVRLESSIKELHDMFMDIAMLVENQGEMLDNIELNVMHTVDHVEKARDETKKAVKYQSQARKKLIIIIVLVVVLLGILALIIGLSVGLN 0E12EBC7CBEDB81E rho-zfyv9-1 2 7 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind full dna sequence full identification by DNA sequencing full dna sequence undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association The bovine SARA protein aligned to residues 729-1425 of the human protein Our two-hybrid screens using 39 residues of the rhodopsin cytoplasmic C terminus as a bait repeatedly isolated a bovine cDNA clone encoding an open reading frame with 97% identity to the C-terminal half (C627-V1323) of human SARA (Figure 1C). 1C rho-zfyv9-2 5 10 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 1E Pull-down assays were carried out by incubating purified His-SARA with either GST- or GST-Rho39-conjugated glutathione Sepharose. rho-zfyv9-3 6 8 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey physical association physical association 1F Furthermore, anti-rhodopsin antibody (Ab), but not control Ab, was able to coimmunoprecipitate endogenous SARA from mouse retinal extracts (Figure 1F), suggesting that SARA interacts with rhodopsin in vivo. rho-zfyv9-4 3 8 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 1G both endogenous SARA (data not shown) and transfected Flag-SARA (Figure 1G) extensively colocalized with EEA1 on early endosomes in HEK cells. Cotransfected rhodopsin was also frequently found on EEA1+/SARA+ early endosomes (Figure 1G) stx3-zfyv9-1 3 12 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 6B and Supp. Fig. 9 The intracellular vesicular myc-syntaxin 3 also colocalized with FLAG-SARA in double transfected HEK cells (Figure 6B). stx3-zfyv9-2 5 10 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig. 6C These experiments showed that purified His-SARA specifically bound to immobilized GST-syntaxin 3 (Figure 6C), stx3-zfyv9-3 1 10 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6D Our results showed that myc-syntaxin 3 was specifically coprecipitated with FLAG-SARA by anti-FLAG Ab (Figure 6D). zfyv9-pi3p-1 4 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait direct interaction direct interaction SARA specifically bound to PI3P in phospholipid filter binding assays. Supp. Fig. 2