IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. J. Neurochem. (0022-3042) 2005 imex curation Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Only protein-protein interactions rat-brain Rattus norvegicus brain brain Brain [cerebrum] far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. J. Neurochem. (0022-3042) 2005 imex curation Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. J. Neurochem. (0022-3042) 2005 imex curation Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. J. Neurochem. (0022-3042) 2005 imex curation Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. J. Neurochem. (0022-3042) 2005 imex curation Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Only protein-protein interactions rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. The scaffolding protein PSD-95 interacts with the glycine transporter GLYT1 and impairs its internalization. J. Neurochem. (0022-3042) 2005 imex curation Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Only protein-protein interactions rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cubelos B., Gonzalez-Gonzalez IM., Gimenez C., Zafra F. Synapse - Interactions of proteins with an established role in the presynapse. check when CV for antibody array CV in place Rat brain synaptosomal extracts were incubated an antibody array membrane wich contains 400 different antibodies. The proteins retained were then incubated with biotinylated anti-GLYT1 antibody and then with spreptavidin peroxidase. The interaction was detected by ECL. fig 1A fzafra@cbm.uam.es Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Neurochem. (0022-3042) 2005 Only protein-protein interactions imex curation sc6a9_rat Sodium- and chloride-dependent glycine transporter 1 Solute carrier family 6 member 9 Slc6a9 protein protein rat Rattus norvegicus (Rat) Rat MAVAHGPVATSSPEQNGAVPSEATKKDQNLTRGNWGNQIEFVLTSVGYAVGLGNVWRFPYLCYRNGGGAFMFPYFIMLVFCGIPLFFMELSFGQFASQGCLGVWRISPMFKGVGYGMMVVSTYIGIYYNVVICIAFYYFFSSMTHVLPWAYCNNPWNTPDCAGVLDASNLTNGSRPTALSGNLSHLFNYTLQRTSPSEEYWRLYVLKLSDDIGDFGEVRLPLLGCLGVSWVVVFLCLIRGVKSSGKVVYFTATFPYVVLTILFVRGVTLEGAFTGIMYYLTPKWDKILEAKVWGDAASQIFYSLGCAWGGLITMASYNKFHNNCYRDSVIISITNCATSVYAGFVIFSILGFMANHLGVDVSRVADHGPGLAFVAYPEALTLLPISPLWSLLFFFMLILLGLGTQFCLLETLVTAIVDEVGNEWILQKKTYVTLGVAVAGFLLGIPLTSQAGIYWLLLMDNYAASFSLVVISCIMCVSIMYIYGHRNYFQDIQMMLGFPPPLFFQICWRFVSPTIIFFILIFTVIQYRPITYNHYQYPGWAVAIGFLMALSSVICIPLYALFQLCRTDGDTLLQRLKNATKPSRDWGPALLEHRTGRYAPTTTPSPEDGFEVQPLHPDKAQIPIVGSNGSSRLQDSRI AB43FCDE767F4AFA dlg4_rat Disks large homolog 4 Dlg4 Dlgh4 Postsynaptic density protein 95 Synapse-associated protein 90 Psd95 protein protein rat Rattus norvegicus (Rat) Rat MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL DIP protein P31016 original sequence version: 86 DIP protein P31016-1 original sequence version: 148 7922D4E8E0F9AD85 p28572-2 Sodium- and chloride-dependent glycine transporter 1 GlyT-1B GlyT-1 Slc6a9 Solute carrier family 6 member 9 protein protein rat Rattus norvegicus (Rat) Rat MAVAHGPVATSSPEQNGAVPSEATKKDQNLTRGNWGNQIEFVLTSVGYAVGLGNVWRFPYLCYRNGGGAFMFPYFIMLVFCGIPLFFMELSFGQFASQGCLGVWRISPMFKGVGYGMMVVSTYIGIYYNVVICIAFYYFFSSMTHVLPWAYCNNPWNTPDCAGVLDASNLTNGSRPTALSGNLSHLFNYTLQRTSPSEEYWRLYVLKLSDDIGDFGEVRLPLLGCLGVSWVVVFLCLIRGVKSSGKVVYFTATFPYVVLTILFVRGVTLEGAFTGIMYYLTPKWDKILEAKVWGDAASQIFYSLGCAWGGLITMASYNKFHNNCYRDSVIISITNCATSVYAGFVIFSILGFMANHLGVDVSRVADHGPGLAFVAYPEALTLLPISPLWSLLFFFMLILLGLGTQFCLLETLVTAIVDEVGNEWILQKKTYVTLGVAVAGFLLGIPLTSQAGIYWLLLMDNYAASFSLVVISCIMCVSIMYIYGHRNYFQDIQMMLGFPPPLFFQICWRFVSPTIIFFILIFTVIQYRPITYNHYQYPGWAVAIGFLMALSSVICIPLYALFQLCRTDGDTLLQRLKNATKPSRDWGPALLEHRTGRYAPTTTPSPEDGFEVQPLHPDKAQIPIVGSNGSSRLQDSRI AB43FCDE767F4AFA ita1_rat Integrin alpha-1 Laminin and collagen receptor VLA-1 CD49 antigen-like family member A Itga1 protein protein rat Rattus norvegicus (Rat) Rat MVPRRPASLEVTVACIWLLTVILGFCVSFNVDVKNSMSFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVGQPKARTGDVYKCPVGRERAMPCVKLDLPVNTSIPNVTEIKENMTFGSTLVTNPNGGFLACGPLYAYRCGHLHYTTGICSDVSPTFQVVNSFAPVQECSTQLDIVIVLDGSNSIYPWESVIAFLNDLLKRMDIGPKQTQVGIVQYGENVTHEFNLNKYSSTEEVLVAANKIGRQGGLQTMTALGIDTARKEAFTEARGARRGVKKVMVIVTDGESHDNYRLKQVIQDCEDENIQRFSIAILGHYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVTIVKALGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKANQMVIPHNTTFQTEPAKMNEPLASYLGYTVNSATIPGDVLYIAGQPRYNHTGQVVIYKMEDGNINILQTLGGEQIGSYFGSVLTTIDIDKDSYTDLLLVGAPMYMGTEKEEQGKVYVYAVNQTRFEYQMSLEPIRQTCCSSLKDNSCTKENKNEPCGARFGTAIAAVKDLNVDGFNDVVIGAPLEDDHAGAVYIYHGSGKTIREAYAQRIPSGGDGKTLKFFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWARDVAVVKVTMNFEPNKVNIQKKNCRVEGKETVCINATMCFHVKLKSKEDSIYEADLQYRVTLDSLRQISRSFFSGTQERKIQRNITVRESECIRHSFYMLDKHDFQDSVRVTLDFNLTDPENGPVLDDALPNSVHEHIPFAKDCGNKERCISDLTLNVSTTEKSLLIVKSQHDKFNVSLTVKNKGDSAYNTRTVVQHSPNLIFSGIEEIQKDSCESNQNITCRVGYPFLRAGETVTFKIIFQFNTSHLSENAIIHLSATSDSEEPLESLNDNEVNISIPVKYEVGLQFYSSASEHHISVAANETIPEFINSTEDIGNEINVFYTIRKRGHFPMPELQLSISFPNLTADGYPVLYPIGWSSSDNVNCRPRSLEDPFGINSGKKMTISKSEVLKRGTIQDCSSTCGVATITCSLLPSDLSQVNVSLLLWKPTFIRAHFSSLNLTLRGELKSENSSLTLSSSNRKRELAIQISKDGLPGRVPLWVILLSAFAGLLLLMLLILALWKIGFFKRPLKKKMEK 8E5DA2BE02362EE4 glyt1-dlg4-1 1 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association glyt1-itga1-1 1 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association glyt1-dlg4-2 6 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association glyt1-dlg4-3 4 9 unspecified role unspecified role bait bait gly-arg-ile sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association P28572-2; P31016 overexpressed Deletion of the last 3 amino-acids or addition of an Alanine at the c-terminus of Glyt1 disrupt the interaction with PSD95. glyt1-dlg4-4 2 9 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-849004]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 639-639, sequence length 638 [EBI-849004]564-639 region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gly-arg-ile sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 physical association physical association glyt1-dlg4-5 3 9 unspecified role unspecified role bait bait c-terminus sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-848997]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 639-639, sequence length 638 [EBI-848997]564-639 undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:21:04 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'gly-arg-ile' 8 unspecified role unspecified role prey prey pdz1 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pdz2 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pdz3 domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association While the strongest interaction was observed with the second PDZ domain, glyt1 also interacts with the first PDZ domain and weakly with the third one. glyt1-dlg4-6 5 8 unspecified role unspecified role neutral component neutral component cfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component undefined mutation mutation disrupting mutation disrupting interaction mutation disrupting deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain Wed Jul 06 17:46:55 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'gly-arg-ile' region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization P28572-2; P31016 over-expressed