IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Phospholipase Cgamma1 controls surface expression of TRPC3 through an intermolecular PH domain. Phospholipase Cgamma1 controls surface expression of TRPC3 through an intermolecular PH domain. Nature (0028-0836) 2005 van Rossum DB., Patterson RL., Sharma S., Barrow RK., Kornberg M., Gill DL., Snyder SH. Synapse - Interactions of proteins with an established role in the presynapse. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot van Rossum DB., Patterson RL., Sharma S., Barrow RK., Kornberg M., Gill DL., Snyder SH. Synapse - Interactions of proteins with an established role in the presynapse. Nature (0028-0836) 2005 Phospholipase Cgamma1 controls surface expression of TRPC3 through an intermolecular PH domain. Phospholipase Cgamma1 controls surface expression of TRPC3 through an intermolecular PH domain. Nature (0028-0836) 2005 van Rossum DB., Patterson RL., Sharma S., Barrow RK., Kornberg M., Gill DL., Snyder SH. Synapse - Interactions of proteins with an established role in the presynapse. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined van Rossum DB., Patterson RL., Sharma S., Barrow RK., Kornberg M., Gill DL., Snyder SH. Synapse - Interactions of proteins with an established role in the presynapse. Nature (0028-0836) 2005 trpc3_human Short transient receptor potential channel 3 Transient receptor protein 3 TRPC3 TRP3 protein protein human Homo sapiens Human MREKGRRQAVRGPAFMFNDRGTSLTAEEERFLDAAEYGNIPVVRKMLEESKTLNVNCVDYMGQNALQLAVGNEHLEVTELLLKKENLARIGDALLLAISKGYVRIVEAILNHPGFAASKRLTLSPCEQELQDDDFYAYDEDGTRFSPDITPIILAAHCQKYEVVHMLLMKGARIERPHDYFCKCGDCMEKQRHDSFSHSRSRINAYKGLASPAYLSLSSEDPVLTALELSNELAKLANIEKEFKNDYRKLSMQCKDFVVGVLDLCRDSEEVEAILNGDLESAEPLEVHRHKASLSRVKLAIKYEVKKFVAHPNCQQQLLTIWYENLSGLREQTIAIKCLVVLVVALGLPFLAIGYWIAPCSRLGKILRSPFMKFVAHAASFIIFLGLLVFNASDRFEGITTLPNITVTDYPKQIFRVKTTQFTWTEMLIMVWVLGMMWSECKELWLEGPREYILQLWNVLDFGMLSIFIAAFTARFLAFLQATKAQQYVDSYVQESDLSEVTLPPEIQYFTYARDKWLPSDPQIISEGLYAIAVVLSFSRIAYILPANESFGPLQISLGRTVKDIFKFMVLFIMVFFAFMIGMFILYSYYLGAKVNAAFTTVEESFKTLFWSIFGLSEVTSVVLKYDHKFIENIGYVLYGIYNVTMVVVLLNMLIAMINSSYQEIEDDSDVEWKFARSKLWLSYFDDGKTLPPPFSLVPSPKSFVYFIMRIVNFPKCRRRRLQKDIEMGMGNSKSRLNLFTQSNSRVFESHSFNSILNQPTRYQQIMKRLIKRYVLKAQVDKENDEVNEGELKEIKQDISSLRYELLEDKSQATEELAILIHKLSEKLNPSMLRCE DIP protein Q13507 original sequence version: 138 4179CB3DA8B3F508 plcg1_rat 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 Phosphoinositide phospholipase C-gamma-1 Phospholipase C-gamma-1 Plcg1 protein protein rat Rattus norvegicus (Rat) Rat MAGVGTPCANGCGPSAPSEAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGSIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAATPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDFEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLETNTLRTGERPELCQVSLSEFQQFLLEYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDELVTFLFSKENSVWNSQLDAVCPETMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQHFRKVLGDTLLTKPVDIAADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSVMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEASGSTELHSSEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFTKSAIIQNVEKQDGGWWRGDYGGKKQLWFPSNYVEEMINPAILEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMPSVAQWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKMMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMAGGHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCVICIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERASDASSQLFHVRAREGSFEARYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL DIP protein P10686 original sequence version: 98 DIP protein P10686 original sequence version: 127 BB3240C27972CE3B plcg1-trcpc3-1 2 3 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q13507:p.Phe31Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A Wed May 02 15:28:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe31ala' Tue Jun 30 18:54:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe31Ala' Q13507:p.Glu28Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 18:51:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu28ala' Tue Jun 30 16:45:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu28Ala' Q13507:p.Leu32Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A Wed May 02 19:21:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu32ala' Tue Jun 30 19:18:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu32Ala' 4 unspecified role unspecified role bait bait ph c domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain physical association physical association plcg1-trcpc3-2 1 4 unspecified role unspecified role bait bait ph c domain binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain deletion required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q13507:p.Phe31Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain F A Wed May 02 16:06:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe31ala' Tue Jun 30 19:34:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe31Ala' Q13507:p.Glu28Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain E A Wed May 02 18:29:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu28ala' Tue Jun 30 18:55:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu28Ala' Q13507:p.Leu32Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:42:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu32ala' Tue Jun 30 16:45:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu32Ala' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 15 18 physical association physical association The PHc domain of PLC gamma 1 is the C-terminal split (861-920) of a PH domain The N-terminal split of the PH domain is in 489-523 fragment. The PHc domain of PLC gamma 1 seems to complement with a PHn domain found in TRPC3. Binding of TRPC3 to PLC gamma change PLC gamma binding specificity to phosphoinositides.