IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism. Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism. Nature communications 2019 imex curation Fox NG., Yu X., Feng X., Bailey HJ., Martelli A., Nabhan JF., Strain-Damerell C., Bulawa C., Yue WW., Han S. Rare diseases - Interactions investigated in the context of Rare genetic disease wyatt.yue@sgc.ox.ac.uk,seungil.han@pfizer.com 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single predetermined predetermined participant predetermined Fox NG., Yu X., Feng X., Bailey HJ., Martelli A., Nabhan JF., Strain-Damerell C., Bulawa C., Yue WW., Han S. Rare diseases - Interactions investigated in the context of Rare genetic disease Suppl. Fig. 2 co-purification experiments were not captured as two participants were his-tagged, could not infer which one could be the bait wyatt.yue@sgc.ox.ac.uk,seungil.han@pfizer.com Accepted 2019-OCT-10 AT 11:01 BST AT 11:01 BST by PPORRAS Nature communications 2019 Only protein-protein interactions imex curation imex curation Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism. Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism. Nature communications 2019 imex curation Fox NG., Yu X., Feng X., Bailey HJ., Martelli A., Nabhan JF., Strain-Damerell C., Bulawa C., Yue WW., Han S. Rare diseases - Interactions investigated in the context of Rare genetic disease wyatt.yue@sgc.ox.ac.uk,seungil.han@pfizer.com 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro dsc differential scanning calorimetry dsc predetermined predetermined participant predetermined Fox NG., Yu X., Feng X., Bailey HJ., Martelli A., Nabhan JF., Strain-Damerell C., Bulawa C., Yue WW., Han S. Rare diseases - Interactions investigated in the context of Rare genetic disease wyatt.yue@sgc.ox.ac.uk,seungil.han@pfizer.com Accepted 2019-OCT-10 AT 11:01 BST AT 11:01 BST by PPORRAS Nature communications 2019 Only protein-protein interactions imex curation imex curation Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism. Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism. Nature communications 2019 imex curation Fox NG., Yu X., Feng X., Bailey HJ., Martelli A., Nabhan JF., Strain-Damerell C., Bulawa C., Yue WW., Han S. Rare diseases - Interactions investigated in the context of Rare genetic disease wyatt.yue@sgc.ox.ac.uk,seungil.han@pfizer.com 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Fox NG., Yu X., Feng X., Bailey HJ., Martelli A., Nabhan JF., Strain-Damerell C., Bulawa C., Yue WW., Han S. Rare diseases - Interactions investigated in the context of Rare genetic disease wyatt.yue@sgc.ox.ac.uk,seungil.han@pfizer.com Accepted 2019-OCT-10 AT 11:01 BST AT 11:01 BST by PPORRAS Nature communications 2019 Only protein-protein interactions imex curation imex curation Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism. Structure of the human frataxin-bound iron-sulfur cluster assembly complex provides insight into its activation mechanism. Nature communications 2019 imex curation Fox NG., Yu X., Feng X., Bailey HJ., Martelli A., Nabhan JF., Strain-Damerell C., Bulawa C., Yue WW., Han S. Rare diseases - Interactions investigated in the context of Rare genetic disease wyatt.yue@sgc.ox.ac.uk,seungil.han@pfizer.com 21-Nov-2019: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Fox NG., Yu X., Feng X., Bailey HJ., Martelli A., Nabhan JF., Strain-Damerell C., Bulawa C., Yue WW., Han S. Rare diseases - Interactions investigated in the context of Rare genetic disease wyatt.yue@sgc.ox.ac.uk,seungil.han@pfizer.com Accepted 2019-OCT-10 AT 11:01 BST AT 11:01 BST by PPORRAS Nature communications 2019 Only protein-protein interactions imex curation imex curation frda_human Frataxin, mitochondrial Friedreich ataxia protein FXN FRDA X25 protein protein human Homo sapiens Human MWTLGRRAVAGLLASPSPAQAQTLTRVPRPAELAPLCGRRGLRTDIDATCTPRRASSNQRGLNQIWNVKKQSVYLMNLRKSGTLGHPGSLDETTYERLAEETLDSLAEFFEDLADKPYTFEDYDVSFGSGVLTVKLGGDLGTYVINKQTPNKQIWLSSPSSGPKRYDWTGKNWVYSHDGVSLHELLAAELTKALKTKLDLSSLAYSGKDA ECC81738779308CF s-dodecanoyl-4'-phosphopantetheine S-dodecanoyl-4'-phosphopantetheine dodecanoyl-4'-phosphopantetheine lauroyl-4-phosphopantetheine S-dodecanoyl-D-pantetheine 4'-phosphate S-lauroyl-4-phosphopantetheine S-lauroyl-D-pantetheine 4'-phosphate S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) nfs1_human Cysteine desulfurase, mitochondrial NFS1 NIFS HUSSY-08 protein protein human Homo sapiens Human MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPADTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH FDE76177DB6E751B zinc(2+) zinc(2+) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) pyridoxal 5'-phosphate pyridoxal 5'-phosphate 3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-4-pyridinecarboxaldehyde 3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde 5-phosphate codecarboxylase Phosphoric acid mono-(4-formyl-5-hydroxy-6-methyl-pyridin-3-ylmethyl) ester pyridoxal 5-monophosphoric acid ester pyridoxal 5'-(dihydrogen phosphate) Pyridoxal 5'-phosphate Pyridoxal 5-phosphate Pyridoxal phosphate PYRIDOXAL-5'-PHOSPHATE (4-formyl-5-hydroxy-6-methylpyridin-3-yl)methyl dihydrogen phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) NGVDGCNFYWLIFO-UHFFFAOYSA-N NGVDGCNFYWLIFO-UHFFFAOYSA-N q9y697-2 Cysteine desulfurase, mitochondrial NFS1 NIFS HUSSY-08 protein protein human Homo sapiens Human MDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH 61DF3AD65D5CDD3C iscu_human Iron-sulfur cluster assembly enzyme ISCU, mitochondrial NifU-like N-terminal domain-containing protein ISCU NIFUN NifU-like protein protein protein human Homo sapiens Human MAAAGAFRLRRAASALLLRSPRLPARELSAPARLYHKKVVDHYENPRNVGSLDKTSKNVGTGLVGAPACGDVMKLQIQVDEKGKIVDARFKTFGCGSAIASSSLATEWVKGKTVEEALTIKNTDIAKELCLPPVKLHCSMLAEDAIKAALADYKLKQEPKKGEAEKK DIP protein Q9H1K1 original sequence version: 140 0166D3EC9F1EEB47 a0a437hbf4_ecolx Acyl carrier protein acpP DQL48_09575 protein protein ecolx Escherichia coli MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELVMALEEEFDTEIPDEEAEKITTVQAAIDYINGH The sequence has been withdrawn from uniprot. 6C39D45BFEA131EE lyrm4_human LYR motif-containing protein 4 LYRM4 C6orf149 ISD11 CGI-203 protein protein human Homo sapiens Human MAASSRAQVLSLYRAMLRESKRFSAYNYRTYAVRRIRDAFRENKNVKDPVEIQTLVNKAKRDLGVIRRQVHIGQLYSTDKLIIENRDMPRT F0C5919CA701F3F3 acpp-fxn-1 1 12 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ISD11 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Zn2+ binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined FXN binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain NFS1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ISCU binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ACP binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NFS1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4'PP binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain FXN binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ISD11 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NFS1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component purified purified acyl chain binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified ISCU binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role neutral component neutral component purified purified NFS1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified ISCU binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified acyl chain binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role neutral component neutral component purified purified NFS1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 12 unspecified role unspecified role neutral component neutral component purified purified ISD11 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component purified purified FXN binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Zn2+ binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified ISCU binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain NFS1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified ACP binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NFS1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 4'PP binding region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain FXN binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ISD11 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined NFS1 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 47 17 30 18 45 21 22 26 25 70 31 71 34 43 35 61 66 36 72 37 53 39 57 41 69 49 63 51 59 55 physical association physical association Supplementary Table 1, Fig. 1a, b, Supplementary Figure 4 3.2Å More specifically, the interaction detection method is Cryo-EM with 3D refinement iscu-acpp-1 4 7 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role prey prey purified purified ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q16595:p.Asp124Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 17:14:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp124Ala' Q16595:p.Asn146Lys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N K Tue Jun 30 19:13:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn146Lys' Q16595:p.Asn151Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N A Tue Jun 30 15:47:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn151Ala' Q16595:p.Trp155Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain W R Tue Jun 30 18:28:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp155Arg' Q16595:p.Pro163Gly mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P G Tue Jun 30 17:57:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro163Gly' Q16595:p.Arg165Cys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R C Tue Jun 30 17:16:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg165Cys' ecoli Escherichia coli (strain K12) physical association physical association Table 1, Supplementary Figure 1d Impact of FXN mutations is on the whole of SDAU complex (NFS1-ISD11-ACP-ISCU); SDAU is not a functional complex without FXN so we couldn't have the possibility of generating a complex portal identifier so as to be taken as a participant fxn-nfs1-2 2 7 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain p.Asp124Ala mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain D A p.Asn146Lys mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain N K p.Asn151Ala mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain N A p.Trp155Arg mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain W R p.Pro163Gly mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain P G p.Arg165Cys mutation with complex effect mutation with complex effect certain certain sequence position certain certain certain sequence position certain R C ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 From the given melting temperature of the individual mutations, it is not possible to infer effects of the mutations. The difference in melting point can tell us about the overall stability of the interaction, but very little detail is available on the binding site acpp-fxn-2 3 12 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Supplementary Table 4, Supplementary Figure 7 0.99 Å acpp-fxn-3 3 12 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role neutral component neutral component purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 6 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role neutral component neutral component purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Supplementary Table 4, Supplementary Figure 7