MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. Frontiers in molecular neuroscience 2018 imex curation Rogers EJ., Jada R., Schragenheim-Rozales K., Sah M., Cortes M., Florence M., Levy NS., Moss R., Walikonis RS., Palty R., Shalgi R., Lichtman D., Kavushansky A., Gerges NZ., Kahn I., Umanah GKE., Levy AP. Rare diseases - Interactions investigated in the context of Rare genetic disease interactions involving Arf6 and GGA3 could not be captured as origin species of the proteins is not available. interactions involving Arf6 and GGA3 could not be captured as origin species of the proteins is not available. alevy@technion.ac.il 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down tag luciferase tag visualisation by luciferase assay tag luciferase Rogers EJ., Jada R., Schragenheim-Rozales K., Sah M., Cortes M., Florence M., Levy NS., Moss R., Walikonis RS., Palty R., Shalgi R., Lichtman D., Kavushansky A., Gerges NZ., Kahn I., Umanah GKE., Levy AP. Rare diseases - Interactions investigated in the context of Rare genetic disease interactions involving Arf6 and GGA3 could not be captured as origin species of the proteins is not available. interactions involving Arf6 and GGA3 could not be captured as origin species of the proteins is not available. alevy@technion.ac.il Accepted 2020-DEC-16 AT 10:42 GMT AT 10:42 GMT by KALPANAP Frontiers in molecular neuroscience 2018 Only protein-protein interactions imex curation imex curation An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. An IQSEC2 Mutation Associated With Intellectual Disability and Autism Results in Decreased Surface AMPA Receptors. Frontiers in molecular neuroscience 2018 imex curation Rogers EJ., Jada R., Schragenheim-Rozales K., Sah M., Cortes M., Florence M., Levy NS., Moss R., Walikonis RS., Palty R., Shalgi R., Lichtman D., Kavushansky A., Gerges NZ., Kahn I., Umanah GKE., Levy AP. Rare diseases - Interactions investigated in the context of Rare genetic disease interactions involving Arf6 and GGA3 could not be captured as origin species of the proteins is not available. interactions involving Arf6 and GGA3 could not be captured as origin species of the proteins is not available. alevy@technion.ac.il 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. lumier luminescence based mammalian interactome mapping lumier tag luciferase tag visualisation by luciferase assay tag luciferase Rogers EJ., Jada R., Schragenheim-Rozales K., Sah M., Cortes M., Florence M., Levy NS., Moss R., Walikonis RS., Palty R., Shalgi R., Lichtman D., Kavushansky A., Gerges NZ., Kahn I., Umanah GKE., Levy AP. Rare diseases - Interactions investigated in the context of Rare genetic disease interactions involving Arf6 and GGA3 could not be captured as origin species of the proteins is not available. interactions involving Arf6 and GGA3 could not be captured as origin species of the proteins is not available. alevy@technion.ac.il Accepted 2020-DEC-16 AT 10:42 GMT AT 10:42 GMT by KALPANAP Frontiers in molecular neuroscience 2018 Only protein-protein interactions imex curation imex curation calm1_human Calmodulin-1 CALM1 CALM CAM CAM1 protein protein human Homo sapiens Human MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK 6B4BC3FCDE10727B calm1_calm2_calm3_human CALM1/CALM2/CALM3 identical protein CALM molecule set molecule set human Homo sapiens Human iqec2_human IQ motif and SEC7 domain-containing protein 2 IQSEC2 KIAA0522 protein protein human Homo sapiens Human MEAGSGPPGGPGSESPNRAVEYLLELNNIIESQQQLLETQRRRIEELEGQLDQLTQENRDLREESQLHRGELHRDPHGARDSPGRESQYQNLRETQFHHRELRESQFHQAARDVGYPNREGAYQNREAVYRDKERDASYPLQDTTGYTARERDVAQCHLHHENPALGRERGGREAGPAHPGREKEAGYSAAVGVGPRPPRERGQLSRGASRSSSPGAGGGHSTSTSTSPATTLQRKSDGENSRTVSVEGDAPGSDLSTAVDSPGSQPPYRLSQLPPSSSHMGGPPAGVGLPWAQRARLQPASVALRKQEEEEIKRSKALSDSYELSTDLQDKKVEMLERKYGGSFLSRRAARTIQTAFRQYRMNKNFERLRSSASESRMSRRIILSNMRMQFSFEEYEKAQNPAYFEGKPASLDEGAMAGARSHRLERGLPYGGSCGGGIDGGGSSVTTSGEFSNDITELEDSFSKQVKSLAESIDEALNCHPSGPMSEEPGSAQLEKRESKEQQEDSSATSFSDLPLYLDDTVPQQSPERLPSTEPPPQGRPEFWAPAPLPPVPPPVPSGTREDGSREEGTRRGPGCLECRDFRLRAAHLPLLTIEPPSDSSVDLSDRSDRGSVHRQLVYEADGCSPHGTLKHKGPPGRAPIPHRHYPAPEGPAPAPPGPLPPAPNSGTGPSGVAGGRRLGKCEAAGENSDGGDNESLESSSNSNETINCSSGSSSRDSLREPPATGLCKQTYQRETRHSWDSPAFNNDVVQRRHYRIGLNLFNKKPEKGIQYLIERGFLSDTPVGVAHFILERKGLSRQMIGEFLGNRQKQFNRDVLDCVVDEMDFSSMDLDDALRKFQSHIRVQGEAQKVERLIEAFSQRYCVCNPALVRQFRNPDTIFILAFAIILLNTDMYSPSVKAERKMKLDDFIKNLRGVDNGEDIPRDLLVGIYQRIQGRELRTNDDHVSQVQAVERMIVGKKPVLSLPHRRLVCCCQLYEVPDPNRPQRLGLHQREVFLFNDLLVVTKIFQKKKILVTYSFRQSFPLVEMHMQLFQNSYYQFGIKLLSAVPGGERKVLIIFNAPSLQDRLRFTSDLRESIAEVQEMEKYRVESELEKQKGMMRPNASQPGGAKDSVNGTMARSSLEDTYGAGDGLKRGALSSSLRDLSDAGKRGRRNSVGSLDSTIEGSVISSPRPHQRMPPPPPPPPPEEYKSQRPVSNSSSFLGSLFGSKRGKGPFQMPPPPTGQASASSSSASSTHHHHHHHHHGHSHGGLGVLPDGQSKLQALHAQYCQGPGPAPPPYLPPQQPSLPPPPQQPPPLPQLGSIPPPPASAPPVGPHRHFHAHGPVPGPQHYTLGRPGRAPRRGAGGHPQFAPHGRHPLHQPTSPLPLYSPAPQHPPAHKQGPKHFIFSHHPQMMPAAGAAGGPGSRPPGGSYSHPHHPQSPLSPHSPIPPHPSYPPLPPPSPHTPHSPLPPTSPHGPLHASGPPGTANPPSANPKAKPSRISTVV DIP protein Q5JU85 original sequence version: 64 52F8B7BE28A175F3 calm-iqsec2-1 1 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate Q5JU85:p.Ala350Val mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A V luc tag renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 4 unspecified role unspecified role bait bait purified purified physical association physical association Fig. 1B-C The binding of calmodulin to wild type and A350V IQSEC2 is increased by calcium. CHEBI:29108 - calcium(2+) calm1_human-iqsec2-1 2 5 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed Q5JU85:p.Ala350Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain A V luc tag renilla luciferase renilla luciferase protein tag renilla luciferase n-term range n-terminal range n-term range n-term range n-terminal range n-term range ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q5JU85:p.Arg359Cys mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R C Q5JU85:p.[Ile354_Gln355delinsAlaAla;Arg359Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain IQ AA certain certain sequence position certain certain certain sequence position certain R A 3 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 2 The binding of calmodulin to wild type and A350V IQSEC2 is increased by calcium. CHEBI:29108 - calcium(2+)