IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction between G Protein-Coupled Receptor 143 and Tyrosinase: Implications for Understanding Ocular Albinism Type 1. Interaction between G Protein-Coupled Receptor 143 and Tyrosinase: Implications for Understanding Ocular Albinism Type 1. The Journal of investigative dermatology 2017 imex curation De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org 04-05-20: Contacted by IntAct-Help. Dr. P Sergouniotis PhD University of Manchester, UK Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org Accepted 2020-JAN-24 AT 14:46 GMT AT 14:46 GMT by PPORRAS The Journal of investigative dermatology 2017 Only protein-protein interactions imex curation imex curation Interaction between G Protein-Coupled Receptor 143 and Tyrosinase: Implications for Understanding Ocular Albinism Type 1. Interaction between G Protein-Coupled Receptor 143 and Tyrosinase: Implications for Understanding Ocular Albinism Type 1. The Journal of investigative dermatology 2017 imex curation De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org 04-05-20: Contacted by IntAct-Help. Dr. P Sergouniotis PhD University of Manchester, UK Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org Accepted 2020-JAN-24 AT 14:46 GMT AT 14:46 GMT by PPORRAS The Journal of investigative dermatology 2017 Only protein-protein interactions imex curation imex curation Interaction between G Protein-Coupled Receptor 143 and Tyrosinase: Implications for Understanding Ocular Albinism Type 1. Interaction between G Protein-Coupled Receptor 143 and Tyrosinase: Implications for Understanding Ocular Albinism Type 1. The Journal of investigative dermatology 2017 imex curation De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org 04-05-20: Contacted by IntAct-Help. Dr. P Sergouniotis PhD University of Manchester, UK Only protein-protein interactions imex curation mouse-melan-a Mouse melan-a - immortalised melanocyte cell line melan-a Mouse melan-a - immortalised melanocyte cell line anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org Accepted 2020-JAN-24 AT 14:46 GMT AT 14:46 GMT by PPORRAS The Journal of investigative dermatology 2017 Only protein-protein interactions imex curation imex curation Interaction between G Protein-Coupled Receptor 143 and Tyrosinase: Implications for Understanding Ocular Albinism Type 1. Interaction between G Protein-Coupled Receptor 143 and Tyrosinase: Implications for Understanding Ocular Albinism Type 1. The Journal of investigative dermatology 2017 imex curation De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org 04-05-20: Contacted by IntAct-Help. Dr. P Sergouniotis PhD University of Manchester, UK Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 confocal microscopy confocal microscopy immunostaining immunostaining De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org Accepted 2020-JAN-24 AT 14:46 GMT AT 14:46 GMT by PPORRAS The Journal of investigative dermatology 2017 Only protein-protein interactions imex curation imex curation Interaction between G Protein-Coupled Receptor 143 and Tyrosinase: Implications for Understanding Ocular Albinism Type 1. Interaction between G Protein-Coupled Receptor 143 and Tyrosinase: Implications for Understanding Ocular Albinism Type 1. The Journal of investigative dermatology 2017 imex curation De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org 04-05-20: Contacted by IntAct-Help. Dr. P Sergouniotis PhD University of Manchester, UK Only protein-protein interactions imex curation mouse-melan-a Mouse melan-a - immortalised melanocyte cell line melan-a Mouse melan-a - immortalised melanocyte cell line confocal microscopy confocal microscopy immunostaining immunostaining De Filippo E., Schiedel AC., Manga P. Rare diseases - Interactions investigated in the context of Rare genetic disease schiedel@uni-bonn.de,Prashiela.Manga@nyumc.org Accepted 2020-JAN-24 AT 14:46 GMT AT 14:46 GMT by PPORRAS The Journal of investigative dermatology 2017 Only protein-protein interactions imex curation imex curation tyro_mouse Tyrosinase Monophenol monooxygenase Albino locus protein Tyr protein protein mouse Mus musculus Mouse MFLAVLYCLLWSFQISDGHFPRACASSKNLLAKECCPPWMGDGSPCGQLSGRGSCQDILLSSAPSGPQFPFKGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFGGPNCTEKRVLIRRNIFDLSVSEKNKFFSYLTLAKHTISSVYVIPTGTYGQMNNGSTPMFNDINIYDLFVWMHYYVSRDTLLGGSEIWRDIDFAHEAPGFLPWHRLFLLLWEQEIRELTGDENFTVPYWDWRDAENCDICTDEYLGGRHPENPNLLSPASFFSSWQIICSRSEEYNSHQVLCDGTPEGPLLRNPGNHDKAKTPRLPSSADVEFCLSLTQYESGSMDRTANFSFRNTLEGFASPLTGIADPSQSSMHNALHIFMNGTMSQVQGSANDPIFLLHHAFVDSIFEQWLRRHRPLLEVYPEANAPIGHNRDSYMVPFIPLYRNGDFFITSKDLGYDYSYLQESDPGFYRNYIEPYLEQASRIWPWLLGAALVGAVIAAALSGLSSRLCLQKKKKKKQPQEERQPLLMDKDDYHSLLYQSHL A4C109A97CBD5D6A gp143_human G-protein coupled receptor 143 GPR143 OA1 Ocular albinism type 1 protein protein protein human Homo sapiens Human MASPRLGTFCCPTRDAATQLVLSFQPRAFHALCLGSGGLRLALGLLQLLPGRRPAGPGSPATSPPASVRILRAAAACDLLGCLGMVIRSTVWLGFPNFVDSVSDMNHTEIWPAAFCVGSAMWIQLLYSACFWWLFCYAVDAYLVIRRSAGLSTILLYHIMAWGLATLLCVEGAAMLYYPSVSRCERGLDHAIPHYVTMYLPLLLVLVANPILFQKTVTAVASLLKGRQGIYTENERRMGAVIKIRFFKIMLVLIICWLSNIINESLLFYLEMQTDINGGSLKPVRTAAKTTWFIMGILNPAQGFLLSLAFYGWTGCSLGFQSPRKEIQWESLTTSAAEGAHPSPLMPHENPASGKVSQVGGQTSDEALSMLSEGSDASTIEIHTASESCNKNEGDPALPTHGDL DIP protein P51810 original sequence version: 116 20DEB20E80CC0E1D tyro_human Tyrosinase LB24-AB Monophenol monooxygenase SK29-AB Tumor rejection antigen AB TYR protein protein human Homo sapiens Human MLLAVLYCLLWSFQTSAGHFPRACVSSKNLMEKECCPPWSGDRSPCGQLSGRGSCQNILLSNAPLGPQFPFTGVDDRESWPSVFYNRTCQCSGNFMGFNCGNCKFGFWGPNCTERRLLVRRNIFDLSAPEKDKFFAYLTLAKHTISSDYVIPIGTYGQMKNGSTPMFNDINIYDLFVWMHYYVSMDALLGGSEIWRDIDFAHEAPAFLPWHRLFLLRWEQEIQKLTGDENFTIPYWDWRDAEKCDICTDEYMGGQHPTNPNLLSPASFFSSWQIVCSRLEEYNSHQSLCNGTPEGPLRRNPGNHDKSRTPRLPSSADVEFCLSLTQYESGSMDKAANFSFRNTLEGFASPLTGIADASQSSMHNALHIYMNGTMSQVQGSANDPIFLLHHAFVDSIFEQWLRRHRPLQEVYPEANAPIGHNRESYMVPFIPLYRNGDFFISSKDLGYDYSYLQDSDPDSFQDYIKSYLEQASRIWSWLLGAAMVGAVLTALLAGLVSLLCRHKRKQLPEEKQPLLMEKEDYHSLYQSHL 67211A91608A59E1 gpr143-tyr-1 1 8 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell P51810:p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain LL AA certain certain sequence position certain certain certain sequence position certain WE AA Tue Jun 30 18:11:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla]' fusion tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2a, S2 gpr143-tyr-2 3 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P51810:p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain LL AA certain certain sequence position certain certain certain sequence position certain WE AA Tue Jun 30 16:55:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla]' 6 western blot western blot Immuno blot Immuno blot anti tag western anti tag western blot anti tag western unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association 2b, S4 gpr143-tyr-3 1 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P51810:p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain LL AA certain certain sequence position certain certain certain sequence position certain WE AA Tue Jun 30 17:06:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla]' physical association physical association S3 gpr143-tyr-4 4 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell region egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell fusion tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P51810:p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain LL AA certain certain sequence position certain certain certain sequence position certain WE AA Tue Jun 30 20:09:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla]' colocalization colocalization 3a gpr143-tyr-5 5 6 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell region eyfp enhanced yellow fluorescent protein tag eyfp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization 3b gpr143-tyr-6 2 7 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept over-expressed over expressed level over-expressed living cell living cell P51810:p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain LL AA certain certain sequence position certain certain certain sequence position certain WE AA Tue Jun 30 17:21:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu223_Leu224delinsAlaAla;Trp329_Glu330delinsAlaAla]' c-terminal region eyfp enhanced yellow fluorescent protein tag eyfp c-term range C-terminal range c-term range c-term range C-terminal range c-term range 8 unspecified role unspecified role fluorescence donor fluorescence donor over-expressed over expressed level over-expressed living cell living cell c-terminal region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range proximity proximity close-contact colocalization neighbourhood interaction 4, 5, S5, S6, S7, S8