IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell-iTreg cell balance. IL-1 signaling modulates activation of STAT transcription factors to antagonize retinoic acid signaling and control the TH17 cell-iTreg cell balance. Nature immunology 2015 imex curation Basu R., Whitley SK., Bhaumik S., Zindl CL., Schoeb TR., Benveniste EN., Pear WS., Hatton RD., Weaver CT. IBD - Inflammatory bowel disease more causality from Supp fig 8 if author reply? email sent 28/2/15 regarding species of IL1-beta.(no Reply) Please make sure ROLES (Bait/Prey) are still present - they seem to come and go at random. I did not ask the authors for species of any other cytokines as I am not sure it is possible to work out what effect they have... cweaver@uab.edu 19-Jan-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Basu R., Whitley SK., Bhaumik S., Zindl CL., Schoeb TR., Benveniste EN., Pear WS., Hatton RD., Weaver CT. IBD - Inflammatory bowel disease ChIP results using the STAT5 antibody cannot be included due to lack of antibody specificity. more causality from Supp fig 8 if author reply? email sent 28/2/15 regarding species of IL1-beta.(no Reply) Please make sure ROLES (Bait/Prey) are still present - they seem to come and go at random. I did not ask the authors for species of any other cytokines as I am not sure it is possible to work out what effect they have... CD4+ single-positive thymocytes from wild-type or Stat5 KO mice activated by anti-CD3/28 and subsequently grown under these conditions:- 1] Th0 conditions - no cytokines added 2] Th17 conditions - IL-6 plus TGF-beta alone 3] Th17 conditions -as in 2] but with or without Retinoic acid(RA) or RA with IL-1Beta, then left unstimulated or by addtion of IL2 plus IL6 (STAT5 assay) or IL6 alone (STAT3 assay). cweaver@uab.edu Accepted 2015-DEC-09 AT 12:29 GMT AT 12:29 GMT by ORCHARD Nature immunology 2015 imex curation il17_mouse_gene Interleukin-17 receptor A gene gene mouse Mus musculus Mouse stat3_mouse Signal transducer and activator of transcription 3 Acute-phase response factor Stat3 Aprf protein protein mouse Mus musculus Mouse MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMDLTSECATSPM DIP protein P42227 original sequence version: 169 DIP protein P42227 original sequence version: 162 DIP protein P42227 original sequence version: 164 DIP protein P42227 original sequence version: 141 6C00626711C8012D foxp3_mouse_gene Forkhead box protein P3 Foxp3 gene gene mouse Mus musculus Mouse il17f_mouse_gene Interleukin-17F gene gene gene mouse Mus musculus Mouse stat3-il17_mouse_gene-1 1 3 unspecified role unspecified role bait bait region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm 2 unspecified role unspecified role prey prey regulatory_and _promoter_sites sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 7A CHEBI:15367 - RA (retinoic acid);except at the enhancer 10 kilobases (kb) downstream of the Il17a transcription start site P42232 -Stat5B P42232 decreased the binding of P42227 to ENSMUSG00000025929 CHEBI:15367 decreased the binding of P42227 to ENSMUSG00000025929 IL-1-beta The addition of IL-1-beta reversed the RA-induced decrease in binding of STAT3. stat3-il17f_mouse_gene-1 1 3 unspecified role unspecified role bait bait region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm 5 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 7A-2 P42232 decreased the binding of P42227 to ENSMUSG00000041872 P42232 -Stat5B CHEBI:15367 decreased the binding of P42227 to ENSMUSG00000041872 IL-1-beta CHEBI:15367 decreases the expression at protein level of ENSMUSG00000041872 CHEBI:15367 - RA (retinoic acid) The addition of IL-1-beta reversed the RA-induced decrease in binding of STAT3. stat3-foxp3-1 1 3 unspecified role unspecified role bait bait region phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm 4 unspecified role unspecified role prey prey cns2_enhancer__region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 7A-3 P42232 -Stat5B The addition of IL-1-beta reversed the RA-induced decrease in binding of STAT3. CHEBI:15367 increases the expression at protein level of ENSMUSG00000039521. CHEBI:15367 decreased the binding of P42227 to ENSMUSG00000039521 P42232 decreased the binding of P42227 to ENSMUSG00000039521 CHEBI:15367 - RA (retinoic acid)