IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Gene expression in the Gitr locus is regulated by NF-κB and Foxp3 through an enhancer. Gene expression in the Gitr locus is regulated by NF-κB and Foxp3 through an enhancer. Journal of immunology (Baltimore, Md. : 1950) 2014 imex curation Tone Y., Kidani Y., Ogawa C., Yamamoto K., Tsuda M., Peter C., Waldmann H., Tone M. IBD - Inflammatory bowel disease Results from Figure 7E have not been included as the origin of the construct "FLAG-tagged Foxp3" is not stated, and the corresponding author did not reply to a request for information. Masahide.Tone@csmc.edu 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift autoradiography autoradiography Tone Y., Kidani Y., Ogawa C., Yamamoto K., Tsuda M., Peter C., Waldmann H., Tone M. IBD - Inflammatory bowel disease Results from Figure 7E have not been included as the origin of the construct "FLAG-tagged Foxp3" is not stated, and the corresponding author did not reply to a request for information. In figure 4 a Nuclear extract from CD3-activated EL4 cells were used. In Supplementary figure 3 a Nuclear extract from non-activated EL4 cells were used. Masahide.Tone@csmc.edu Accepted 2015-NOV-19 AT 10:58 GMT AT 10:58 GMT by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2014 Only protein-protein interactions imex curation imex curation I have removed "EBI-11293443" - leaving (EBI-11293697); Expt-4 (fig 7E) deleted and a comment added a Publication level. I have altered the gene- mouse one Gene expression in the Gitr locus is regulated by NF-κB and Foxp3 through an enhancer. Gene expression in the Gitr locus is regulated by NF-κB and Foxp3 through an enhancer. Journal of immunology (Baltimore, Md. : 1950) 2014 imex curation Tone Y., Kidani Y., Ogawa C., Yamamoto K., Tsuda M., Peter C., Waldmann H., Tone M. IBD - Inflammatory bowel disease Results from Figure 7E have not been included as the origin of the construct "FLAG-tagged Foxp3" is not stated, and the corresponding author did not reply to a request for information. Masahide.Tone@csmc.edu 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Tone Y., Kidani Y., Ogawa C., Yamamoto K., Tsuda M., Peter C., Waldmann H., Tone M. IBD - Inflammatory bowel disease Results from Figure 7E have not been included as the origin of the construct "FLAG-tagged Foxp3" is not stated, and the corresponding author did not reply to a request for information. causality statements that could be inferred from EL4 cells have not been added, despite the authors claim that these cells "known to retain many features of T cells". T cells activated by anti-CD3, also CD4+CD25− T cells, nTreg cells and induced TReg cells. Masahide.Tone@csmc.edu Accepted 2015-NOV-19 AT 10:58 GMT AT 10:58 GMT by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2014 Only protein-protein interactions imex curation imex curation I have removed "EBI-11293443" - leaving (EBI-11293697); Expt-4 (fig 7E) deleted and a comment added a Publication level. I have altered the gene- mouse one Gene expression in the Gitr locus is regulated by NF-κB and Foxp3 through an enhancer. Gene expression in the Gitr locus is regulated by NF-κB and Foxp3 through an enhancer. Journal of immunology (Baltimore, Md. : 1950) 2014 imex curation Tone Y., Kidani Y., Ogawa C., Yamamoto K., Tsuda M., Peter C., Waldmann H., Tone M. IBD - Inflammatory bowel disease Results from Figure 7E have not been included as the origin of the construct "FLAG-tagged Foxp3" is not stated, and the corresponding author did not reply to a request for information. Masahide.Tone@csmc.edu 03-Dec-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Tone Y., Kidani Y., Ogawa C., Yamamoto K., Tsuda M., Peter C., Waldmann H., Tone M. IBD - Inflammatory bowel disease Results from Figure 7E have not been included as the origin of the construct "FLAG-tagged Foxp3" is not stated, and the corresponding author did not reply to a request for information. I have added two versions for this figure:- 7 B/Cii-A and 7 B/Cii-B -are two different ways of showing Fig 7C right-hand image; probably only one is appropriate to add? CD4+CD25− T cells stimulated with anti-CD3/28 and TFG-beta, or unstimulated CD4+CD25− T cells. Sequence of the "kB" (kB1 + kB2 regions) probe used was deduced from one primer stated in methods section (GAAACACC ACAGGTGGGACA) and 101bp added to this sequence using sequence given in Fig 3A. kB1 and FoxP3 Probe sequences were taken from Fig 7A. Masahide.Tone@csmc.edu Accepted 2015-NOV-19 AT 10:58 GMT AT 10:58 GMT by ORCHARD Journal of immunology (Baltimore, Md. : 1950) 2014 Only protein-protein interactions imex curation imex curation I have removed "EBI-11293443" - leaving (EBI-11293697); Expt-4 (fig 7E) deleted and a comment added a Publication level. I have altered the gene- mouse one bcl3_mouse B-cell lymphoma 3 protein homolog Bcl3 protein protein mouse Mus musculus Mouse MPRCPAGAMDEGPVDLRTRPKGTPGAALPLRKRPLRPASPEPATTRSPAGPLDALRSGCDVPVVPGPPHCVARPEALYYQGPLMPIYSTPTMAPHFPLLNLPTHPYSMICPMEHPLSADIAMATRVDEDGDTPLHIAVVQNNIAAVYRILSLFKLGSREVDVHNNLRQTPLHLAVITTLPDMVRLLVTAGASPMALDRHGQTAIHLACEHRSPSCLQALLDSATSGSVDLEVRNYEGLTALHVAVNTGCQEAVLLLLERGADIDAVDIKSGRSPLIHAVENNSLNMVQLLLLHGANVNAQMYSGSSALHSASGRGLLPLVRTLVRSGADSGLKNCHNDTPLMVARSRRVIDILRGKASRAASGSQPEPSPDQSATNSPESSSRLSSNGLQSSPSSSPSLSPPKDAPGFPATPQNFFLPTTSTPAFLPFPGVLRGPGRPVPPSPAPGSS 7665D28BBC3EE194 sp1_mouse Transcription factor Sp1 Sp1 Specificity protein 1 protein protein mouse Mus musculus Mouse MSDQDHSMDEVTAVVKIEKDVGGNNGGSGNGGGAAFSQTRSSSTGSSSSSGGGGGQESQPSPLALLAATCSRIESPNENSNNSQGPSQSGGTGELDLTATQLSQGANGWQIISSSSGATPTSKEQSGNSTNGSNGSESSKNRTVSGGQYVVAATPNLQNQQVLTGLPGVMPNIQYQVIPQFQTVDGQQLQFAATGAQVQQDGSGQIQIIPGANQQIIPNRGSGGNIIAAMPNLLQQAVPLQGLANNVLSGQTQYVTNVPVALNGNITLLPVNSVSAATLTPSSQAGTISSSGSQESSSQPVTSGTAISSASLVSSQASSSSFFTNANSYSTTTTTSNMGIMNFTSSGSSGTSSQGQTPQRVGGLQGSDSLNIQQNQTSGGSLQGSQQKEGEQSQQTQQQQILIQPQLVQGGQALQALQAAPLSGQTFTTQAISQETLQNLQLQAVQNSGPIIIRTPTVGPNGQVSWQTLQLQNLQVQNPQAQTITLAPMQGVSLGQTSSSNTTLTPIASAASIPAGTVTVNAAQLSSMPGLQTINLSALGTSGIQVHQLPGLPLAIANTPGDHGTQLGLHGSGGDGIHDETAGGEGENSSDLQPQAGRRTRREACTCPYCKDSEGRASGDPGKKKQHICHIQGCGKVYGKTSHLRAHLRWHTGERPFMCNWSYCGKRFTRSDELQRHKRTHTGEKKFACPECPKRFMRSDHLSKHIKTHQNKKGGPGVALSVGTLPLDSGAGSEGTATPSALITTNMVAMEAICPEGIARLANSGINVMQVTELQSINISGNGF DIP protein O89090 original sequence version: 112 B6B989F0A252CEE5 tf65_mouse Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit Rela Nfkb3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein mouse Mus musculus Mouse MDDLFPLIFPSEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGYYEADLCPDRSIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFHVPIEEQRGDYDLNAVRLCFQVTVRDPAGRPLLLTPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFNGPTEPRPPTRRIAVPTRNSTSVPKPAPQPYTFPASLSTINFDEFSPMLLPSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTPGPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSEFQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDEDFSSIADMDFSALLSQISS DIP protein Q04207 original sequence version: 158 DIP protein Q04207 original sequence version: 117 DIP protein Q04207-1 original sequence version: 117 DIP protein Q04207-1 original sequence version: 118 DIP protein Q04207 original sequence version: 119 DIP protein Q04207 original sequence version: 141 DIP protein Q04207-1 original sequence version: 141 DIP protein Q04207-1 original sequence version: 149 DIP protein Q04207 original sequence version: 149 DIP protein Q04207 original sequence version: 168 DIP protein Q04207 original sequence version: 125 BDF1673D2FE398B9 cd40/nfkb_competitor_probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid mouse Mus musculus Mouse CCCTGGGAAGGTCCTGGGGAAATCCCTGCT competitor_b1_probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid mouse Mus musculus Mouse AGGTGGGACATCCCCAAACAGATGA tnr18_mouse_gene Tumor necrosis factor receptor superfamily member 18 Tnfrsf18 Gitr gene gene mouse Mus musculus Mouse foxp3_competitor_probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid mouse Mus musculus Mouse CCCAAACAGAATGACGTGTGTGCAAAATACCCA nfkb_b1_site_probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid mouse Mus musculus Mouse CACCACAGGTGGGACATCCCCAAACAGA nfkb_b2_site_probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid mouse Mus musculus Mouse ATGTGCTGGAAACCCCTCCTGTGGGG p25799-pro_0000030313 Nuclear factor NF-kappa-B p50 subunit Nfkb1 DNA-binding factor KBF1 EBP-1 NF-kappa-B1 p84/NF-kappa-B1 p98 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 protein protein mouse Mus musculus Mouse MADDDPYGTGQMFHLNTALTHSIFNAELYSPEIPLSTDGPYLQILEQPKQRGFRFRYVCEGPSHGGLPGASSEKNKKSYPQVKICNYVGPAKVIVQLVTNGKNIHLHAHSLVGKHCEDGVCTVTAGPKDMVVGFANLGILHVTKKKVFETLEARMTEACIRGYNPGLLVHSDLAYLQAEGGGDRQLTDREKEIIRQAAVQQTKEMDLSVVRLMFTAFLPDSTGSFTRRLEPVVSDAIYDSKAPNASNLKIVRMDRTAGCVTGGEEIYLLCDKVQKDDIQIRFYEEEENGGVWEGFGDFSPTDVHRQFAIVFKTPKYKDVNITKPASVFVQLRRKSDLETSEPKPFLYYPEIKDKEEVQRKRQKLMPNFSDSFGGGSGAGAGGGGMFGSGGGGGSTGSPGPGYGYSNYGFPPYGGITFHPGVTKSNAGVTHG 0597F8EA07635A94 1 431 0597F8EA07635A94 nfkb_b3_site_probe dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid mouse Mus musculus Mouse TTAGATCTGGGGATTTCCACTAAG kb_probe_enhancer_b1+b2_sites dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid mouse Mus musculus Mouse GAAACACCACAGGTGGGACATCCCCAAACAGATGACGTGTGTGCAAATACCCACAGGCTGTATGTGCTGGAAACCCCTCCTGTGGGCGGCTG sp3_mouse Transcription factor Sp3 Sp3 protein protein mouse Mus musculus Mouse MTAPEKPVKQEEMAALDVDGGGGGGGHGEYLQQQQQQQQQHGNGAAAAAAQDTQPSPLALLAATCSKIGPPSPGDDDEEAAVAAAAGVPAAAAGATGDLASAQLGGAPNRWEVLSATPTTIKDEAGNLVQIPGAATSSGQYVLPLQNLQNQQIFSVAPGSDSSNGTVSNVQYQVIPQIQSTDAQQVQIGFTGSSDNGGINQENSQIQIIPGSNQTLLASGTPPANIQNLIPQTGQVQVQGVAIGGSSFPGQTQVVANVPLGLPGNITFVPINSVDLDSLGLSGSSQTMTAGINADGHLINTGQAMDSSDNSERTGERVSPDVNETNADTDLFVPTSSSSQLPVTIDSTGILQQNTNSLTTTSGQVHSSDLQGNYIQSPVSEETQAQNIQVSTAQPVVQHLQLQDSQQPTSQAQIVQGITPQTIHGVQASGQNISQQALQNLQLQLNPGTFLIQAQTVTPSGQITWQTFQVQGVQNLQNLQIQNTAAQQITLTPVQTLTLGQVAAGGALTSTPVSLSTGQLPNLQTVTVNSIDSTGIQLHPGENADSPADIRIKEEEPDPEEWQLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYGKTSHLRAHLRWHSGERPFICNWMFCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTHQNKKVIHSSSTVLASVEAGRDDALITAGGTTLILANIQQGSVSGIGTVNTSATSNQDILTNTEIPLQLVTVSGNETME E45A00D566454D61 foxp3_mouse Forkhead box protein P3 Foxp3 Scurfin protein protein mouse Mus musculus Mouse MPNPRPAKPMAPSLALGPSPGVLPSWKTAPKGSELLGTRGSGGPFQGRDLRSGAHTSSSLNPLPPSQLQLPTVPLVMVAPSGARLGPSPHLQALLQDRPHFMHQLSTVDAHAQTPVLQVRPLDNPAMISLPPPSAATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPRSGTPRKDSNLLAAPQGSYPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPSVASMDKSSCCIVATSTQGSVLPAWSAPREAPDGGLFAVRRHLWGSHGNSSFPEFFHNMDYFKYHNMRPPFTYATLIRWAILEAPERQRTLNEIYHWFTRMFAYFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDEFEFRKKRSQRPNKCSNPCP DIP protein Q99JB6 original sequence version: 120 DIP protein Q99JB6 original sequence version: 110 DIP protein Q99JB6 original sequence version: 114 DIP protein Q99JB6 original sequence version: 102 DIP protein Q99JB6 original sequence version: 100 28D5B8E67891840C rel_mouse Proto-oncogene c-Rel Rel protein protein mouse Mus musculus Mouse MASSGYNPYVEIIEQPRQRGMRFRYKCEGRSAGSIPGERSTDNNRTYPSVQIMNYYGKGKIRITLVTKNDPYKPHPHDLVGKDCRDGYYEAEFGPERRPLFFQNLGIRCVKKKEVKGAIILRISAGINPFNVGEQQLLDIEDCDLNVVRCVFMFFLPDEDGNFTTALPPIVSNPIYDNRAPNTAELRICRVNKNCGSVRGGDEIFLLCDKVQKDDIEVRFVLNDWEARGVFSQADVHRQVAIVFKTPPYCKAILEPVTVKMQLRRPSDQEVSESMDFRYLPDEKDAYGNKSKKQKTTLIFQKLLQDCGHFTEKPRTAPLGSTGEGRFIKKESNLFSHGTVLPEMPRSSGVPGQAEPYYSSCGSISSGLPHHPPAIPSVAHQPTSWPPVTHPTSHPVSTNTLSTFSAGTLSSNSQGILPFLEGPGVSDLSASNSCLYNPDDLARMETPSMSPTDLYSISDVNMLSTRPLSVMAPSTDGMGDTDNPRLVSINLENPSCNARLGPRDLRQLHQMSPASLSAGTSSSSVFVSQSDAFDRSNFSCVDNGLMNEPGLSDDANNPTFVQSSHYSVNTLQSEQLSDPFTYGFFKI DIP protein P15307 original sequence version: 88 98FC237B6D140416 h4_mouse Histone H4 H4c11 H4c12 H4c14 H4c2 H4c3 H4c4 H4c6 H4c8 H4c9 H4f16 Hist1h4a Hist4h4 Hist1h4b Hist1h4c Hist1h4d Hist1h4f Hist1h4h Hist1h4i Hist1h4j Hist1h4k Hist2h4a Hist2h4 Hist1h4m H4-53 H4-12 H4c1 protein protein mouse Mus musculus Mouse MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG DIP protein P62806 original sequence version: 108 A9E5DFD3F8B97598 hist1h4h-tnfsf18-1 2 9 unspecified role unspecified role prey prey B1+B2_sites_nfkb_binding_region_of enhancer sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B3_site_nfkb_binding_region_of enhancer sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role bait bait region ac1res monoacetylated residue Acetyl Acetylation (N terminus, N epsilon of Lysine, O of Serine) (Ac) Acetylation Ac1Res undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm association association 2 F/G Histone H4 was highly acetylated in nTreg and T cells activated by anti-CD3 treatment, but not in naive CD4+CD25− T cells. nfkb_b1_region_probe-nfkb1-2 1 13 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line 11 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4C nfkb_b1_region_probe-rela-1 1 6 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line 11 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4Ci nfkb_b2_site_probe-nfkb1-2 1 13 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line 12 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4Cii nfkb_b2_site_probe-nfkb1-1 1 6 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line 12 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4Ciii nfkb_b3_site_probe-rela-1 1 6 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line 14 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4Civ nfkb_b3_site_probe-rela-2 1 13 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line 14 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4Cv nfkb1-tnfsf18-1 2 9 unspecified role unspecified role prey prey B1+B2_sites_nfkb_binding_region_of enhancer sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B3_site_nfkb_binding_region_of enhancer sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role bait bait association association 5A, 6 A/B p50 binding to the kB1 plus κB2 and kB3 regions was increased in T cells 24 h after CD3 activation. Binding was detected in nTreg and induced TReg cells, but not in naive CD4+CD25– T cells. A NF-kB activation inhibitor, identity unknown, reduces the expression at protein level of ENSMUSG00000066755 Causality from Figs 5B( the text meaning imho is not clear for this figure - but you can clearly see a shift to the right for GITR in Wt and KO populations for CD3 stimulated cells. Fig 5F,6C P25799-PRO_0000030313 increases expression at the protein level of ENSMUSG00000041954 (in anti-CD3 stimulated or Foxp3+ cells) kb_probe_enhancer_b1+b2_sites-foxp3-1 3 15 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ 13 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ 4 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ 18 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ 6 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ association association 7 B/C Non-stimulated cells showed no binding. kb_probe_enhancer_b1+b2_sites-bcl3-1 3 8 competitor competitor competitor prey prey 15 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ 13 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ 4 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ 18 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ 6 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ association association 7 B/Ci kb_probe_enhancer_b1+b2_sites-bcl3-3 3 7 competitor competitor competitor prey prey 15 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ association association 7 D Foxp3 binding was only minimally affected by competition from a probe containing a p50 binding site;however p50 binding was not completely inhibited. kb_probe_enhancer_b1+b2_sites-bcl3-4 3 10 competitor competitor competitor prey prey 15 unspecified role unspecified role bait bait 5-prime biotin tag biotin tag certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role prey prey mouse-t-cell_cd4+ Mouse CD4 positive T-cell t-cell_cd4+ t cell cd4+ association association 7 B/Cii-B I am not 100% sure this is the best way to show this; p50 binding to the enhancer probe was reduced as a result of inhibition of Foxp3 binding; however binding of Foxp3, p65,c-Rel and Bcl-3 was abolished. nfkb_b1_site_probe-nfkb1-1 1 5 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line 11 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp 3B nfkb_b2_site_probe-sp3-1 1 16 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line 12 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp 3B nfkb_b1_site_probe-sp1-1 1 11 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line physical association physical association Supp 3Bi nfkb_b2_site_probe-sp3-2 1 12 unspecified role unspecified role bait bait region 32p radiolabel 32p radiolabel P32 32P 32P P32 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey mouse-el4 Mus musculus el4 Mouse ascites lymphoma lymphoblast line physical association physical association Supp 3Bii