IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ MafB is a downstream target of the IL-10/STAT3 signaling pathway, involved in the regulation of macrophage de-activation. MafB is a downstream target of the IL-10/STAT3 signaling pathway, involved in the regulation of macrophage de-activation. Biochimica et biophysica acta 2014 imex curation Gemelli C., Zanocco Marani T., Bicciato S., Mazza EM., Boraschi D., Salsi V., Zappavigna V., Parenti S., Selmi T., Tagliafico E., Ferrari S., Grande A. IBD - Inflammatory bowel disease The authors have confirmed that IL-10 was of Human origin. claudia.gemelli@unimore.it 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro emsa supershift electrophoretic mobility supershift assay emsa supershift autoradiography autoradiography Gemelli C., Zanocco Marani T., Bicciato S., Mazza EM., Boraschi D., Salsi V., Zappavigna V., Parenti S., Selmi T., Tagliafico E., Ferrari S., Grande A. IBD - Inflammatory bowel disease The authors have confirmed that IL-10 was of Human origin. claudia.gemelli@unimore.it Accepted 2016-NOV-09 AT 10:17 GMT AT 10:17 GMT by PPORRAS Biochimica et biophysica acta 2014 Only protein-protein interactions imex curation imex curation MafB is a downstream target of the IL-10/STAT3 signaling pathway, involved in the regulation of macrophage de-activation. MafB is a downstream target of the IL-10/STAT3 signaling pathway, involved in the regulation of macrophage de-activation. Biochimica et biophysica acta 2014 imex curation Gemelli C., Zanocco Marani T., Bicciato S., Mazza EM., Boraschi D., Salsi V., Zappavigna V., Parenti S., Selmi T., Tagliafico E., Ferrari S., Grande A. IBD - Inflammatory bowel disease The authors have confirmed that IL-10 was of Human origin. claudia.gemelli@unimore.it 20-Dec-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-monocyte Human mononuclear cells monocyte Monocyte, mononuclear cell ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Gemelli C., Zanocco Marani T., Bicciato S., Mazza EM., Boraschi D., Salsi V., Zappavigna V., Parenti S., Selmi T., Tagliafico E., Ferrari S., Grande A. IBD - Inflammatory bowel disease CD14+ monocytes from adult peripheral blood, treated +/- IL-10. The authors have confirmed that IL-10 was of Human origin. claudia.gemelli@unimore.it Accepted 2016-NOV-09 AT 10:17 GMT AT 10:17 GMT by PPORRAS Biochimica et biophysica acta 2014 Only protein-protein interactions imex curation imex curation mmp9_human_gene Human MMP9 gene;Matrix metalloproteinase-9 gene gene human Homo sapiens Human il7ra_human_gene Interleukin-7 receptor subunit alpha IL7R CDw127 gene gene human Homo sapiens Human mmp9_mare _probe ds dna double stranded deoxyribonucleic acid ds DNA ds dna human Homo sapiens Human CCCTGAGTCAGCA mafb_human_gene Transcription factor MafB KRML MAFB gene gene human Homo sapiens Human mafb_human Transcription factor MafB MAFB KRML V-maf musculoaponeurotic fibrosarcoma oncogene homolog B protein protein human Homo sapiens Human MAAELSMGPELPTSPLAMEYVNDFDLLKFDVKKEPLGRAERPGRPCTRLQPAGSVSSTPLSTPCSSVPSSPSFSPTEQKTHLEDLYWMASNYQQMNPEALNLTPEDAVEALIGSHPVPQPLQSFDSFRGAHHHHHHHHPHPHHAYPGAGVAHDELGPHAHPHHHHHHQASPPPSSAASPAQQLPTSHPGPGPHATASATAAGGNGSVEDRFSDDQLVSMSVRELNRHLRGFTKDEVIRLKQKRRTLKNRGYAQSCRYKRVQQKHHLENEKTQLIQQVEQLKQEVSRLARERDAYKVKCEKLANSGFREAGSTSDSPSSPEFFL A0F3C09F8936CB16 il-7r_mare _probe ds dna double stranded deoxyribonucleic acid ds DNA ds dna human Homo sapiens Human GACTGACTCAGCA stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D DIP protein P40763 original sequence version: 157 DIP protein P40763-1 original sequence version: 147 DIP protein P40763-1 original sequence version: 135 DIP protein P40763-1 original sequence version: 157 DIP protein P40763 original sequence version: 165 DIP protein P40763 original sequence version: 182 DIP protein P40763 original sequence version: 135 6C00632211C8012D mmp9_mare _probe-mafb-1 1 7 unspecified role unspecified role prey prey cell lysate cell lysate in vitro translated in vitro translated protein in vitro translated n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro 5 unspecified role unspecified role bait bait 5-prime 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain G7C,A13T mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G C certain certain sequence position certain certain certain sequence position certain A T physical association physical association 3A-1 mmp9_mare _probe-mafb-2 1 7 unspecified role unspecified role prey prey cell lysate cell lysate in vitro translated in vitro translated protein in vitro translated n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro 8 unspecified role unspecified role bait bait 5-prime 32p radiolabel 32p radiolabel P32 32P 32P P32 certain certain sequence position certain certain certain sequence position certain C7G,C12G mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C G certain certain sequence position certain certain certain sequence position certain C G physical association physical association 3A-2 mafb-mmp9_human_gene-1 2 7 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey promoter_mare_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3B P22301 increases the expression at RNA level of ENSG00000100985 mafb-il7r-1 2 7 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey promoter_mare_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3Bi P22301 increases the expression at RNA level of ENSG00000168685 stat3-mafb-1 2 6 unspecified role unspecified role prey prey enhancer_region_1 sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait association association 6B IL-10 P22301 increases the expression at RNA level of ENSG00000204103 P22301 increases the expression at protein level of ENSG00000204103 Caus. from Fig 4C/D + fig 5