IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The autoimmunity-associated gene PTPN22 potentiates toll-like receptor-driven, type 1 interferon-dependent immunity. The autoimmunity-associated gene PTPN22 potentiates toll-like receptor-driven, type 1 interferon-dependent immunity. Immunity 2013 imex curation Wang Y., Shaked I., Stanford SM., Zhou W., Curtsinger JM., Mikulski Z., Shaheen ZR., Cheng G., Sawatzke K., Campbell AM., Auger JL., Bilgic H., Shoyama FM., Schmeling DO., Balfour HH., Hasegawa K., Chan AC., Corbett JA., Binstadt BA., Mescher MF., Ley K., Bottini N., Peterson EJ. IBD - Inflammatory bowel disease nunzio@liai.org,peter899@umn.edu 19-Jan-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-mddc Human Monocyte-derived dendritic cell monocyte-derived_dendritic_cell Monocyte-derived dendritic cell (MDDC) anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang Y., Shaked I., Stanford SM., Zhou W., Curtsinger JM., Mikulski Z., Shaheen ZR., Cheng G., Sawatzke K., Campbell AM., Auger JL., Bilgic H., Shoyama FM., Schmeling DO., Balfour HH., Hasegawa K., Chan AC., Corbett JA., Binstadt BA., Mescher MF., Ley K., Bottini N., Peterson EJ. IBD - Inflammatory bowel disease nunzio@liai.org,peter899@umn.edu Accepted 2015-DEC-09 AT 13:20 GMT AT 13:20 GMT by ORCHARD Immunity 2013 Only protein-protein interactions imex curation imex curation I dont understand either - removed fuzzies.; altered The autoimmunity-associated gene PTPN22 potentiates toll-like receptor-driven, type 1 interferon-dependent immunity. The autoimmunity-associated gene PTPN22 potentiates toll-like receptor-driven, type 1 interferon-dependent immunity. Immunity 2013 imex curation Wang Y., Shaked I., Stanford SM., Zhou W., Curtsinger JM., Mikulski Z., Shaheen ZR., Cheng G., Sawatzke K., Campbell AM., Auger JL., Bilgic H., Shoyama FM., Schmeling DO., Balfour HH., Hasegawa K., Chan AC., Corbett JA., Binstadt BA., Mescher MF., Ley K., Bottini N., Peterson EJ. IBD - Inflammatory bowel disease nunzio@liai.org,peter899@umn.edu 19-Jan-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Wang Y., Shaked I., Stanford SM., Zhou W., Curtsinger JM., Mikulski Z., Shaheen ZR., Cheng G., Sawatzke K., Campbell AM., Auger JL., Bilgic H., Shoyama FM., Schmeling DO., Balfour HH., Hasegawa K., Chan AC., Corbett JA., Binstadt BA., Mescher MF., Ley K., Bottini N., Peterson EJ. IBD - Inflammatory bowel disease the Zarnegar ref does not state the origin of TRAF3, but gives 2 links - one to a human TRAF3 and one to a mouse TRAF3 = (A002309; ref. 12)-Ref 12 is human and no mention of a Flag tag PTPN22 R/W variants were obtained from "PBMC cDNA"; as PBMCs were human cells, this protein has been assumed to be of human origin. GST-TRAF3 is of Murine origin;(Zarnegar et al., 2008) as this construct was derived "derived by subcloning Flag-TRAF 3". Previous work by the first author Y. Wang (Zhang et al., 2012 pmid:23150880) uses murine TRAF3. nunzio@liai.org,peter899@umn.edu Accepted 2015-DEC-09 AT 13:20 GMT AT 13:20 GMT by ORCHARD Immunity 2013 Only protein-protein interactions imex curation imex curation I dont understand either - removed fuzzies.; altered The autoimmunity-associated gene PTPN22 potentiates toll-like receptor-driven, type 1 interferon-dependent immunity. The autoimmunity-associated gene PTPN22 potentiates toll-like receptor-driven, type 1 interferon-dependent immunity. Immunity 2013 imex curation Wang Y., Shaked I., Stanford SM., Zhou W., Curtsinger JM., Mikulski Z., Shaheen ZR., Cheng G., Sawatzke K., Campbell AM., Auger JL., Bilgic H., Shoyama FM., Schmeling DO., Balfour HH., Hasegawa K., Chan AC., Corbett JA., Binstadt BA., Mescher MF., Ley K., Bottini N., Peterson EJ. IBD - Inflammatory bowel disease nunzio@liai.org,peter899@umn.edu 19-Jan-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wang Y., Shaked I., Stanford SM., Zhou W., Curtsinger JM., Mikulski Z., Shaheen ZR., Cheng G., Sawatzke K., Campbell AM., Auger JL., Bilgic H., Shoyama FM., Schmeling DO., Balfour HH., Hasegawa K., Chan AC., Corbett JA., Binstadt BA., Mescher MF., Ley K., Bottini N., Peterson EJ. IBD - Inflammatory bowel disease nunzio@liai.org,peter899@umn.edu Accepted 2015-DEC-09 AT 13:20 GMT AT 13:20 GMT by ORCHARD Immunity 2013 Only protein-protein interactions imex curation imex curation I dont understand either - removed fuzzies.; altered traf3_mouse TNF receptor-associated factor 3 CD40 receptor-associated factor 1 TRAFAMN Traf3 Craf1 Trafamn RING-type E3 ubiquitin transferase TRAF3 protein protein mouse Mus musculus Mouse MESSKKMDAAGTLQPNPPLKLQPDRGAGSVLVPEQGGYKEKFVKTVEDKYKCEKCRLVLCNPKQTECGHRFCESCMAALLSSSSPKCTACQESIIKDKVFKDNCCKREILALQVYCRNEGRGCAEQLTLGHLLVHLKNECQFEELPCLRADCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIKLQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQAARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP DIP protein Q60803 original sequence version: 127 DIP protein Q60803 original sequence version: 129 DIP protein Q60803 original sequence version: 117 DIP protein Q60803 original sequence version: 121 EC282EFFF84E5A3F ptn22_human Tyrosine-protein phosphatase non-receptor type 22 PTPN22 PTPN8 Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP Lymphoid phosphatase PEST-domain phosphatase protein protein human Homo sapiens Human MDQREILQKFLDEAQSKKITKEEFANEFLKLKRQSTKYKADKTYPTTVAEKPKNIKKNRYKDILPYDYSRVELSLITSDEDSSYINANFIKGVYGPKAYIATQGPLSTTLLDFWRMIWEYSVLIIVMACMEYEMGKKKCERYWAEPGEMQLEFGPFSVSCEAEKRKSDYIIRTLKVKFNSETRTIYQFHYKNWPDHDVPSSIDPILELIWDVRCYQEDDSVPICIHCSAGCGRTGVICAIDYTWMLLKDGIIPENFSVFSLIREMRTQRPSLVQTQEQYELVYNAVLELFKRQMDVIRDKHSGTESQAKHCIPEKNHTLQADSYSPNLPKSTTKAAKMMNQQRTKMEIKESSSFDFRTSEISAKEELVLHPAKSSTSFDFLELNYSFDKNADTTMKWQTKAFPIVGEPLQKHQSLDLGSLLFEGCSNSKPVNAAGRYFNSKVPITRTKSTPFELIQQRETKEVDSKENFSYLESQPHDSCFVEMQAQKVMHVSSAELNYSLPYDSKHQIRNASNVKHHDSSALGVYSYIPLVENPYFSSWPPSGTSSKMSLDLPEKQDGTVFPSSLLPTSSTSLFSYYNSHDSLSLNSPTNISSLLNQESAVLATAPRIDDEIPPPLPVRTPESFIVVEEAGEFSPNVPKSLSSAVKVKIGTSLEWGGTSEPKKFDDSVILRPSKSVKLRSPKSELHQDRSSPPPPLPERTLESFFLADEDCMQAQSIETYSTSYPDTMENSTSSKQTLKTPGKSFTRSKSLKILRNMKKSICNSCPPNKPAESVQSNNSSSFLNFGFANRFSKPKGPRNPPPTWNI DIP protein Q9Y2R2 original sequence version: 69 1ABE8AE89C9D9FBF traf3_human TNF receptor-associated factor 3 TRAF3 CAP1 CRAF1 CD40 receptor-associated factor 1 CD40-binding protein LMP1-associated protein 1 CAP-1 RING-type E3 ubiquitin transferase TRAF3 protein protein human Homo sapiens Human MESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP DIP protein Q13114 original sequence version: 158 DIP protein Q13114 original sequence version: 127 DIP protein Q13114 original sequence version: 146 DIP protein Q13114 original sequence version: 151 9456E440C0A90FBF ptpn22-traf3-1 2 4 unspecified role unspecified role prey prey n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 6C causality from Fig 6F Q9Y2R2 increases the (K63) ubiquitination of Q60803 ptpn22-traf3-2 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 6A ptpn22-traf3-3 1 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 6B ptpn22-traf3-4 3 4 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region_including_Traf_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp 5B traf3-ptpn22-1 3 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp 5C traf3-ptpn22-2 3 4 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp 5D traf3-ptpn22-3 3 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9Y2R2:p.Arg620Trp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R W Wed May 02 17:03:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg620trp' Tue Jun 30 16:45:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg620Trp' physical association physical association Supp 5F The R620W mutation in the PTPN22 gene (rs2476601) s strongly associated with increased risk of human autoimmune and infectious disorders.