I2D I2D Identification and characterization of receptor-interacting protein 2 as a TNFR-associated factor 3 binding partner. Identification and characterization of receptor-interacting protein 2 as a TNFR-associated factor 3 binding partner. Gene (0378-1119) 2013 imex curation Cai X., Du J., Liu Y., Xia W., Liu J., Zou M., Wang Y., Wang M., Su H., Xu D. IBD - Inflammatory bowel disease Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease suhang_lr@sohu.com,xudg@bmi.ac.cn 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Cai X., Du J., Liu Y., Xia W., Liu J., Zou M., Wang Y., Wang M., Su H., Xu D. IBD - Inflammatory bowel disease Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease suhang_lr@sohu.com,xudg@bmi.ac.cn Accepted 2013-DEC-05 by ORCHARD Human Spleen cDNA Gene (0378-1119) 2013 Only protein-protein interactions imex curation Identification and characterization of receptor-interacting protein 2 as a TNFR-associated factor 3 binding partner. Identification and characterization of receptor-interacting protein 2 as a TNFR-associated factor 3 binding partner. Gene (0378-1119) 2013 imex curation Cai X., Du J., Liu Y., Xia W., Liu J., Zou M., Wang Y., Wang M., Su H., Xu D. IBD - Inflammatory bowel disease Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease suhang_lr@sohu.com,xudg@bmi.ac.cn 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Cai X., Du J., Liu Y., Xia W., Liu J., Zou M., Wang Y., Wang M., Su H., Xu D. IBD - Inflammatory bowel disease Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease suhang_lr@sohu.com,xudg@bmi.ac.cn Accepted 2013-DEC-05 by ORCHARD Human Spleen cDNA Gene (0378-1119) 2013 Only protein-protein interactions imex curation Identification and characterization of receptor-interacting protein 2 as a TNFR-associated factor 3 binding partner. Identification and characterization of receptor-interacting protein 2 as a TNFR-associated factor 3 binding partner. Gene (0378-1119) 2013 imex curation Cai X., Du J., Liu Y., Xia W., Liu J., Zou M., Wang Y., Wang M., Su H., Xu D. IBD - Inflammatory bowel disease Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease suhang_lr@sohu.com,xudg@bmi.ac.cn 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-ramos Human Caucasian Burkitt's lymphoma ramos Human Caucasian Burkitt's lymphoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cai X., Du J., Liu Y., Xia W., Liu J., Zou M., Wang Y., Wang M., Su H., Xu D. IBD - Inflammatory bowel disease Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease suhang_lr@sohu.com,xudg@bmi.ac.cn Accepted 2013-DEC-05 by ORCHARD Human Spleen cDNA Gene (0378-1119) 2013 Only protein-protein interactions imex curation Identification and characterization of receptor-interacting protein 2 as a TNFR-associated factor 3 binding partner. Identification and characterization of receptor-interacting protein 2 as a TNFR-associated factor 3 binding partner. Gene (0378-1119) 2013 imex curation Cai X., Du J., Liu Y., Xia W., Liu J., Zou M., Wang Y., Wang M., Su H., Xu D. IBD - Inflammatory bowel disease Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease suhang_lr@sohu.com,xudg@bmi.ac.cn 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Cai X., Du J., Liu Y., Xia W., Liu J., Zou M., Wang Y., Wang M., Su H., Xu D. IBD - Inflammatory bowel disease Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease suhang_lr@sohu.com,xudg@bmi.ac.cn Accepted 2013-DEC-05 by ORCHARD Human Spleen cDNA Gene (0378-1119) 2013 Only protein-protein interactions imex curation Identification and characterization of receptor-interacting protein 2 as a TNFR-associated factor 3 binding partner. Identification and characterization of receptor-interacting protein 2 as a TNFR-associated factor 3 binding partner. Gene (0378-1119) 2013 imex curation Cai X., Du J., Liu Y., Xia W., Liu J., Zou M., Wang Y., Wang M., Su H., Xu D. IBD - Inflammatory bowel disease Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease suhang_lr@sohu.com,xudg@bmi.ac.cn 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Cai X., Du J., Liu Y., Xia W., Liu J., Zou M., Wang Y., Wang M., Su H., Xu D. IBD - Inflammatory bowel disease Crohn's disease - Interactions of proteins identified as having a link to Crohn's disease suhang_lr@sohu.com,xudg@bmi.ac.cn Accepted 2013-DEC-05 by ORCHARD Human Spleen cDNA Gene (0378-1119) 2013 Only protein-protein interactions imex curation ripk2_human Receptor-interacting serine/threonine-protein kinase 2 RIPK2 CARDIAK RICK RIP2 UNQ277/PRO314/PRO34092 RIP-like-interacting CLARP kinase Receptor-interacting protein 2 CARD-containing interleukin-1 beta-converting enzyme-associated kinase Tyrosine-protein kinase RIPK2 protein protein human Homo sapiens Human MNGEAICSALPTIPYHKLADLRYLSRGASGTVSSARHADWRVQVAVKHLHIHTPLLDSERKDVLREAEILHKARFSYILPILGICNEPEFLGIVTEYMPNGSLNELLHRKTEYPDVAWPLRFRILHEIALGVNYLHNMTPPLLHHDLKTQNILLDNEFHVKIADFGLSKWRMMSLSQSRSSKSAPEGGTIIYMPPENYEPGQKSRASIKHDIYSYAVITWEVLSRKQPFEDVTNPLQIMYSVSQGHRPVINEESLPYDIPHRARMISLIESGWAQNPDERPSFLKCLIELEPVLRTFEEITFLEAVIQLKKTKLQSVSSAIHLCDKKKMELSLNIPVNHGPQEESCGSSQLHENSGSPETSRSLPAPQDNDFLSRKAQDCYFMKLHHCPGNHSWDSTISGSQRAAFCDHKTTPCSSAIINPLSTAGNSERLQPGIAQQWIQSKREDIVNQMTEACLNQSLDALLSRDLIMKEDYELVSTKPTRTSKVRQLLDTTDIQGEEFAKVIVQKLKDNKQMGLQPYPEILVVSRSPSLNLLQNKSM DIP protein O43353 original sequence version: 159 DIP protein O43353-1 original sequence version: 116 DIP protein O43353 original sequence version: 116 575A692239505792 traf3_human TNF receptor-associated factor 3 TRAF3 CAP1 CRAF1 CD40 receptor-associated factor 1 CD40-binding protein LMP1-associated protein 1 CAP-1 RING-type E3 ubiquitin transferase TRAF3 protein protein human Homo sapiens Human MESSKKMDSPGALQTNPPLKLHTDRSAGTPVFVPEQGGYKEKFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVFKDNCCKREILALQIYCRNESRGCAEQLMLGHLLVHLKNDCHFEELPCVRPDCKEKVLRKDLRDHVEKACKYREATCSHCKSQVPMIALQKHEDTDCPCVVVSCPHKCSVQTLLRSELSAHLSECVNAPSTCSFKRYGCVFQGTNQQIKAHEASSAVQHVNLLKEWSNSLEKKVSLLQNESVEKNKSIQSLHNQICSFEIEIERQKEMLRNNESKILHLQRVIDSQAEKLKELDKEIRPFRQNWEEADSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQVLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLNGDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKPDPNSSSFKKPTGEMNIASGCPVFVAQTVLENGTYIKDDTIFIKVIVDTSDLPDP DIP protein Q13114 original sequence version: 158 DIP protein Q13114 original sequence version: 127 DIP protein Q13114 original sequence version: 146 DIP protein Q13114 original sequence version: 151 9456E440C0A90FBF traf3-ripk2 2 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association traf3-ripk2-3 1 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig 1a traf3-ripk2-4 5 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 2 traf3-ripk2-5 4 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig 3 Q13114 decreases activity of O43353 O43353 decreases quantity of Q13114 traf3-ripk2-6 3 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig 4