IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ cAMP-responsive element modulator α (CREMα) contributes to decreased Notch-1 expression in T cells from patients with active systemic lupus erythematosus (SLE). cAMP-responsive element modulator α (CREMα) contributes to decreased Notch-1 expression in T cells from patients with active systemic lupus erythematosus (SLE). J. Biol. Chem. (0021-9258) 2012 imex curation Rauen T., Grammatikos A.P., Hedrich C.M., Floege J., Tenbrock K., Ohl K., Kyttaris V.C., Tsokos G.C. IBD - Inflammatory bowel disease The human Notch1 promoter construct is described in PMID:19717984. The CREM-alpha expression construct is described in PMID:15691874 and PMID:11932306. gtsokos@bidmc.harvard.edu 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-t-cell Homo sapiens t-cell Peripheral blood T-lymphocytes ch-ip chromatin immunoprecipitation assay ch-ip q-pcr quantitative pcr q-pcr Rauen T., Grammatikos A.P., Hedrich C.M., Floege J., Tenbrock K., Ohl K., Kyttaris V.C., Tsokos G.C. IBD - Inflammatory bowel disease The human Notch1 promoter construct is described in PMID:19717984. The CREM-alpha expression construct is described in PMID:15691874 and PMID:11932306. gtsokos@bidmc.harvard.edu Accepted 2015-JAN-15 by ORCHARD J. Biol. Chem. (0021-9258) 2012 Only protein-protein interactions imex curation notc1_human_gene Neurogenic locus notch homolog protein 1 (NOTCH1) human gene gene gene human Homo sapiens Human crem_human cAMP-responsive element modulator CREM Inducible cAMP early repressor protein protein human Homo sapiens Human MTMETVESQHDGSITASLTESKSAHVQTQTGQNSIPALAQVSVAGSGTRRGSPAVTLVQLPSGQTIHVQGVIQTPQPWVIQSSEIHTVQVAAIAETDESAESEGVIDSHKRREILSRRPSYRKILNELSSDVPGVPKIEEERSEEEGTPPSIATMAVPTSIYQTSTGQYIAIAQGGTIQISNPGSDGVQGLQALTMTNSGAPPPGATIVQYAAQSADGTQQFFVPGSQVVVQDEETELAPSHMAAATGDMPTYQIRAPTAALPQGVVMAASPGSLHSPQQLAEEATRKRELRLMKNREAAKECRRRKKEYVKCLESRVAVLEVQNKKLIEELETLKDICSPKTDY 4DDEE62904CDD0E6 crem-notc1-1 1 3 unspecified role unspecified role bait bait 2 unspecified role unspecified role prey prey promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5b, 5c Chromatin was immunoprecipitated from total T cells purified from four matched SLE/control pairs (patients with Systemic Lupus Erythematosus versus healthy individuals). Binding of CREM-alpha to the Notch1 promoter was significantly increased in T cells from SLE patients. Q03060 decreases expression at RNA level of ENSG00000148400