IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Deacetylase inhibition promotes the generation and function of regulatory T cells. Deacetylase inhibition promotes the generation and function of regulatory T cells. Nature medicine 2007 imex curation Tao R., de Zoeten EF., Ozkaynak E., Chen C., Wang L., Porrett PM., Li B., Turka LA., Olson EN., Greene MI., Wells AD., Hancock WW. IBD - Inflammatory bowel disease whancock@mail.med.upenn.edu 21-10-2015: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-t-cell Mouse T cell t-cell Peripheral blood T-lymphocytes ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Tao R., de Zoeten EF., Ozkaynak E., Chen C., Wang L., Porrett PM., Li B., Turka LA., Olson EN., Greene MI., Wells AD., Hancock WW. IBD - Inflammatory bowel disease The Mehthods section says this is RT-PCR/qPCR - but the image does looks to be more like -PCR, so entered as this. I have not added nay Causaltiy statements regarding acetylation of HDACs as the the antibody used is a "pan" acetyl-lysine ab - so no idea what it is binding to. CD4+ CD25+ T cells whancock@mail.med.upenn.edu Accepted 2015-OCT-07 AT 15:45 BST AT 15:45 BST by ORCHARD Nature medicine 2007 Only protein-protein interactions imex curation imex curation I have altered the causality statement to reflect what was shown in fig 4A - "CHEBI:46024 increases expression at the protein level of Q99JB6" rather than 'CHEBI:46024 increases expression at the protein level of IL2 gene'. il2_mouse_gene Interleukin-2 Il2 Il-2 gene gene mouse Mus musculus Mouse foxp3_mouse Forkhead box protein P3 Foxp3 Scurfin protein protein mouse Mus musculus Mouse MPNPRPAKPMAPSLALGPSPGVLPSWKTAPKGSELLGTRGSGGPFQGRDLRSGAHTSSSLNPLPPSQLQLPTVPLVMVAPSGARLGPSPHLQALLQDRPHFMHQLSTVDAHAQTPVLQVRPLDNPAMISLPPPSAATGVFSLKARPGLPPGINVASLEWVSREPALLCTFPRSGTPRKDSNLLAAPQGSYPLLANGVCKWPGCEKVFEEPEEFLKHCQADHLLDEKGKAQCLLQREVVQSLEQQLELEKEKLGAMQAHLAGKMALAKAPSVASMDKSSCCIVATSTQGSVLPAWSAPREAPDGGLFAVRRHLWGSHGNSSFPEFFHNMDYFKYHNMRPPFTYATLIRWAILEAPERQRTLNEIYHWFTRMFAYFRNHPATWKNAIRHNLSLHKCFVRVESEKGAVWTVDEFEFRKKRSQRPNKCSNPCP DIP protein Q99JB6 original sequence version: 120 DIP protein Q99JB6 original sequence version: 110 DIP protein Q99JB6 original sequence version: 114 DIP protein Q99JB6 original sequence version: 102 DIP protein Q99JB6 original sequence version: 100 28D5B8E67891840C foxp3-il2-3 1 3 unspecified role unspecified role bait bait Q99JB6:p.[Lys382His;Lys393His] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K H certain certain sequence position certain certain certain sequence position certain K H Wed May 02 17:12:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys382his,lys393his' Tue Jun 30 17:35:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys382His;Lys393His]' undefined mutation mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Wed Jul 06 16:51:20 BST 2016 Sequence change details about this feature cannot be ascertained or do not fit with the current version of the referenced protein, so they have been deleted as a result of our quality control procedures. The original label was 'lys415his' 2 unspecified role unspecified role prey prey promoter_region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 4 C/H CHEBI:46024 ; TSA-trichostatin A - an HDAC inhibitor CHEBI:46024 increases binding of Q99JB6 to ENSMUSG00000027720 mutation mapping has been entered using the sequence given in Figure 4D rather than from the main body of the text, hence the K383 mutation is entered as K382. Q99JB6 decreases expression at the protein level of ENSMUSG00000027720 causality Fig 4A, 4I CHEBI:46024 increases expression at the protein level of Q99JB6