IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Protein kinase Cdelta regulates p67phox phosphorylation in human monocytes. Protein kinase Cdelta regulates p67phox phosphorylation in human monocytes. J. Leukoc. Biol. (0741-5400) 2005 imex curation Zhao X., Xu B., Bhattacharjee A., Oldfield C.M., Wientjes F.B., Feldman G.M., Cathcart M.K. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease The origin of p67phox expression plasmids is described in PMID:1512217. The expression system for human recombinant PKC-delta was obtained from the supplier's website (Upstate Biotechnology). cathcam@ccf.org 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-monocyte Human mononuclear cells monocyte Monocyte, mononuclear cell anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zhao X., Xu B., Bhattacharjee A., Oldfield C.M., Wientjes F.B., Feldman G.M., Cathcart M.K. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis The origin of p67phox expression plasmids is described in PMID:1512217. The expression system for human recombinant PKC-delta was obtained from the supplier's website (Upstate Biotechnology). cathcam@ccf.org Accepted 2015-JAN-06 by ORCHARD J. Leukoc. Biol. (0741-5400) 2005 Only protein-protein interactions imex curation Protein kinase Cdelta regulates p67phox phosphorylation in human monocytes. Protein kinase Cdelta regulates p67phox phosphorylation in human monocytes. J. Leukoc. Biol. (0741-5400) 2005 imex curation Zhao X., Xu B., Bhattacharjee A., Oldfield C.M., Wientjes F.B., Feldman G.M., Cathcart M.K. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis IBD - Inflammatory bowel disease The origin of p67phox expression plasmids is described in PMID:1512217. The expression system for human recombinant PKC-delta was obtained from the supplier's website (Upstate Biotechnology). cathcam@ccf.org 22-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay autoradiography autoradiography Zhao X., Xu B., Bhattacharjee A., Oldfield C.M., Wientjes F.B., Feldman G.M., Cathcart M.K. Ulcerative colitis - Interactions of proteins identified as having a link to ulcerative colitis The origin of p67phox expression plasmids is described in PMID:1512217. The expression system for human recombinant PKC-delta was obtained from the supplier's website (Upstate Biotechnology). cathcam@ccf.org Accepted 2015-JAN-06 by ORCHARD J. Leukoc. Biol. (0741-5400) 2005 Only protein-protein interactions imex curation pkctide Protein kinase C (PKC) consensus substrate peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ERMRPRKRQGSVRRRV ncf2_human Neutrophil cytosol factor 2 NOXA2 P67PHOX Neutrophil NADPH oxidase factor 2 NADPH oxidase activator 2 67 kDa neutrophil oxidase factor p67-phox NCF2 protein protein human Homo sapiens Human MSLVEAISLWNEGVLAADKKDWKGALDAFSAVQDPHSRICFNIGCMYTILKNMTEAEKAFTRSINRDKHLAVAYFQRGMLYYQTEKYDLAIKDLKEALIQLRGNQLIDYKILGLQFKLFACEVLYNIAFMYAKKEEWKKAEEQLALATSMKSEPRHSKIDKAMECVWKQKLYEPVVIPVGKLFRPNERQVAQLAKKDYLGKATVVASVVDQDSFSGFAPLQPQAAEPPPRPKTPEIFRALEGEAHRVLFGFVPETKEELQVMPGNIVFVLKKGNDNWATVMFNGQKGLVPCNYLEPVELRIHPQQQPQEESSPQSDIPAPPSSKAPGRPQLSPGQKQKEEPKEVKLSVPMPYTLKVHYKYTVVMKTQPGLPYSQVRDMVSKKLELRLEHTKLSYRPRDSNELVPLSEDSMKDAWGQVKNYCLTLWCENTVGDQGFPDEPKESEKADANNQTTEPQLKKGSQVEALFSYEATQPEDLEFQEGDIILVLSKVNEEWLEGECKGKVGIFPKVFVEDCATTDLESTRREV DIP protein P19878 original sequence version: 148 EC136766E1915376 kpcd_human Protein kinase C delta type nPKC-delta PRKCD Tyrosine-protein kinase PRKCD PKCD protein protein human Homo sapiens Human MAPFLRIAFNSYELGSLQAEDEANQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQIVLMRAAEEPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMSVQYFLEDVDCKQSMRSEDEAKFPTMNRRGAIKQAKIHYIKNHEFIATFFGQPTFCSVCKDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTAANSRDTIFQKERFNIDMPHRFKVHNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCREKVANLCGINQKLLAEALNQVTQRASRRSDSASSEPVGIYQGFEKKTGVAGEDMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED DIP protein Q05655 original sequence version: 92 DIP protein Q05655 original sequence version: 171 013F4314A2EE331A ncf2-prkcd 2 5 enzyme enzyme neutral component neutral component spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. 4 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation Fig. 4c Q05655 is required for phosphorylation of P19878 P19878 phosphorylation CHEBI:37671 increases binding of Q05655 to P19878 Q05655 increases activity of P19878 CHEBI:37671 indirectly increases activity of P19878 prkcd-1 2 5 putative self putative self putative self putative self spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation true Fig. 4c Q05655 is required for phosphorylation of Q05655 Q05655 phosphorylation pkctide-prkcd 2 3 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 5 enzyme enzyme neutral component neutral component spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. phosphorylation phosphorylation reaction phosphorylation Fig. 4c EBI-10040557 ser-11 O-phospho-L-serine prkcd-ncf2 1 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association Fig. 3a zymosan (opsonised) (ZOP) (CHEBI:37671) No p67phox co-immunoprecipitated with anti-PKC-delta antibodies from cells not treated with ZOP. ncf2-prkcd-2 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association Fig. 3b zymosan (opsonised) (ZOP) (CHEBI:37671) No PKC-delta co-immunoprecipitated with anti-p67phox antibodies from cells not treated with ZOP.