IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC-1alpha) regulates triglyceride metabolism by activation of the nuclear receptor FXR. Peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC-1alpha) regulates triglyceride metabolism by activation of the nuclear receptor FXR. Genes & development 2004 imex curation Zhang Y., Castellani LW., Sinal CJ., Gonzalez FJ., Edwards PA. IBD - Inflammatory bowel disease several Data-Proc/other comments have not been saved, and I cannot remember which...(nomenclature in this paper and Zhang - 2003 differs) GST-PGC-1a was determined to be O70343-1, from reference Puigserver et al. 1998 (PMID:9529258). FXR "alpha3" was determined to be UniProt Q60641-1-as it is also known as "beta-1" which as Figure 1 in Zhang-2003 shows, has 488 residues. The "alpha-4" protein was determined to be Q60641-2 - with 484 residues. The identity of the other two FXR isoforms (lengths 451 and 447aas) does not correspond to any isoform in UniProt, but is probably Q60641-3 and Q60641-4. (474 and 470 residues). As the identity/mapping of these proteins is uncertain, no results obtained using these isoforms have been entered. The FXR DBD is aas 127-203 from Zhang 2003, Figure 1. (refers to the 488 residue protein) jingami@mfour.med.kyoto-u.ac.jp,pedwards@mednet.ucla.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Zhang Y., Castellani LW., Sinal CJ., Gonzalez FJ., Edwards PA. IBD - Inflammatory bowel disease several Data-Proc/other comments have not been saved, and I cannot remember which...(nomenclature in this paper and Zhang - 2003 differs) GST-PGC-1a was determined to be O70343-1, from reference Puigserver et al. 1998 (PMID:9529258). FXR "alpha3" was determined to be UniProt Q60641-1-as it is also known as "beta-1" which as Figure 1 in Zhang-2003 shows, has 488 residues. The "alpha-4" protein was determined to be Q60641-2 - with 484 residues. The identity of the other two FXR isoforms (lengths 451 and 447aas) does not correspond to any isoform in UniProt, but is probably Q60641-3 and Q60641-4. (474 and 470 residues). As the identity/mapping of these proteins is uncertain, no results obtained using these isoforms have been entered. The FXR DBD is aas 127-203 from Zhang 2003, Figure 1. (refers to the 488 residue protein) jingami@mfour.med.kyoto-u.ac.jp,pedwards@mednet.ucla.edu jingami@mfour.med.kyoto-u.ac.jp Accepted 2016-MAY-19 AT 08:02 BST AT 08:02 BST by ORCHARD Genes & development 2004 Only protein-protein interactions imex curation imex curation Q60641-3 replaces D3YTT2; as before I have not added the other 2 isoforms as unsure what their sequence/identity is. Peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC-1alpha) regulates triglyceride metabolism by activation of the nuclear receptor FXR. Peroxisome proliferator-activated receptor-gamma coactivator 1alpha (PGC-1alpha) regulates triglyceride metabolism by activation of the nuclear receptor FXR. Genes & development 2004 imex curation Zhang Y., Castellani LW., Sinal CJ., Gonzalez FJ., Edwards PA. IBD - Inflammatory bowel disease several Data-Proc/other comments have not been saved, and I cannot remember which...(nomenclature in this paper and Zhang - 2003 differs) GST-PGC-1a was determined to be O70343-1, from reference Puigserver et al. 1998 (PMID:9529258). FXR "alpha3" was determined to be UniProt Q60641-1-as it is also known as "beta-1" which as Figure 1 in Zhang-2003 shows, has 488 residues. The "alpha-4" protein was determined to be Q60641-2 - with 484 residues. The identity of the other two FXR isoforms (lengths 451 and 447aas) does not correspond to any isoform in UniProt, but is probably Q60641-3 and Q60641-4. (474 and 470 residues). As the identity/mapping of these proteins is uncertain, no results obtained using these isoforms have been entered. The FXR DBD is aas 127-203 from Zhang 2003, Figure 1. (refers to the 488 residue protein) jingami@mfour.med.kyoto-u.ac.jp,pedwards@mednet.ucla.edu 22-Jun-2016: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zhang Y., Castellani LW., Sinal CJ., Gonzalez FJ., Edwards PA. IBD - Inflammatory bowel disease several Data-Proc/other comments have not been saved, and I cannot remember which...(nomenclature in this paper and Zhang - 2003 differs) GST-PGC-1a was determined to be O70343-1, from reference Puigserver et al. 1998 (PMID:9529258). FXR "alpha3" was determined to be UniProt Q60641-1-as it is also known as "beta-1" which as Figure 1 in Zhang-2003 shows, has 488 residues. The "alpha-4" protein was determined to be Q60641-2 - with 484 residues. The identity of the other two FXR isoforms (lengths 451 and 447aas) does not correspond to any isoform in UniProt, but is probably Q60641-3 and Q60641-4. (474 and 470 residues). As the identity/mapping of these proteins is uncertain, no results obtained using these isoforms have been entered. The FXR DBD is aas 127-203 from Zhang 2003, Figure 1. (refers to the 488 residue protein) jingami@mfour.med.kyoto-u.ac.jp,pedwards@mednet.ucla.edu jingami@mfour.med.kyoto-u.ac.jp Accepted 2016-MAY-19 AT 08:02 BST AT 08:02 BST by ORCHARD Genes & development 2004 Only protein-protein interactions imex curation imex curation Q60641-3 replaces D3YTT2; as before I have not added the other 2 isoforms as unsure what their sequence/identity is. q60641-2 Bile acid receptor Nr1h4 Bar Fxr Rip14 Farnesoid X-activated receptor Farnesol receptor HRR-1 Nuclear receptor subfamily 1 group H member 4 Retinoid X receptor-interacting protein 14 FXFRalpha4 FXRalpha2(-) FXRbeta2 protein protein mouse Mus musculus Mouse MVMQFQGLENPIQISLHHSHRLSGFVPEGMSVKPAKGMLTEHAAGPLGQNLDLESYSPYNNVPFPQVQPQISSSSYYSNLGFYPQQPEDWYSPGIYELRRMPAETGYQGETEVSEMPVTKKPRMAAASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECLLTEIQCKSKRLRKNVKQHADQTANEDDSEGRDLRQVTSTTKFCREKTELTADQQTLLDYIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATSHVQILVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPAGHADLLEERIRKSGISDEYITPMFSFYKSVGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKMYQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ Produced by alternative splicing of isoform 1. 06B56494305A438A o70343-1 Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Ppargc1a Pgc1 Pgc1a Ppargc1 PGC-1a1 protein protein mouse Mus musculus Mouse MAWDMCSQDSVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNEPANIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGAVTTDNEASPSSMPDGTPPPQEAEEPSLLKKLLLAPANTQLSYNECSGLSTQNHAANHTHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELLKYLTTNDDPPHTKPTENRNSSRDKCASKKKSHTQPQSQHAQAKPTTLSLPLTPESPNDPKGSPFENKTIERTLSVELSGTAGLTPPTTPPHKANQDNPFKASPKLKPSCKTVVPPPTKRARYSECSGTQGSHSTKKGPEQSELYAQLSKSSGLSRGHEERKTKRPSLRLFGDHDYCQSLNSKTDILINISQELQDSRQLDFKDASCDWQGHICSSTDSGQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPCQAVFDDKSDKTSELRDGDFSNEQFSKLPVFINSGLAMDGLFDDSEDESDKLSYPWDGTQPYSLFDVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEAYEHERLKRDEYRKEHEKRESERAKQRERQKQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDTNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR AE73EE2B3A622B05 q60641-1 Bile acid receptor Nr1h4 Bar Fxr Rip14 Farnesoid X-activated receptor Farnesol receptor HRR-1 Nuclear receptor subfamily 1 group H member 4 Retinoid X receptor-interacting protein 14 FXRalpha2(+) FXRalpha3 FXRbeta1 protein protein mouse Mus musculus Mouse MVMQFQGLENPIQISLHHSHRLSGFVPEGMSVKPAKGMLTEHAAGPLGQNLDLESYSPYNNVPFPQVQPQISSSSYYSNLGFYPQQPEDWYSPGIYELRRMPAETGYQGETEVSEMPVTKKPRMAAASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECMYTGLLTEIQCKSKRLRKNVKQHADQTANEDDSEGRDLRQVTSTTKFCREKTELTADQQTLLDYIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATSHVQILVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPAGHADLLEERIRKSGISDEYITPMFSFYKSVGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKMYQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ Produced by alternative promoter usage. 3E59B7146F8ECC86 ppargc1a-nr1h4-2 1 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 4 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association 4 B/C/F This is the interaction with the "alpha-3" form of FXR which has 488 residues - See figure 4A. nr1h4-ppargc1a-1 1 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range dbd_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association 4 D/E ppargc1a-nr1h4-3 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4G ppargc1a-nr1h4-6 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4G-2 ppargc1a-nr1h4-9 1 3 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 4 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 31 34 physical association physical association 4B/F This is the interaction with the "alpha-4" form of FXR which has 484 residues.