IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Last Enzyme of the De Novo Purine Synthesis Pathway 5-aminoimidazole-4-carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC) Plays a Central Role in Insulin Signaling and the Golgi/Endosomes Protein Network. The Last Enzyme of the De Novo Purine Synthesis Pathway 5-aminoimidazole-4-carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC) Plays a Central Role in Insulin Signaling and the Golgi/Endosomes Protein Network. Molecular & cellular proteomics : MCP 2015 imex curation Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL., Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL. Diabetes - Interactions investigated in the context of Diabetes Checker: please when accepted please send email to Martial.Boutchueng@crchudequebec.ulaval.ca to let him know it has been curated etc...,with the usual request that next time he submits ahead of publication. robert.faure@crchul.ulaval.ca Boutchueng-Djidjou M Only protein-protein interactions imex curation rat-liver rattus norvegicus liver Rat liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL., Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL. Diabetes - Interactions investigated in the context of Diabetes Checker: please when accepted please send email to Martial.Boutchueng@crchudequebec.ulaval.ca to let him know it has been curated etc...,with the usual request that next time he submits ahead of publication. The Golgi/Endosome fraction containing Insulin receptor complexes was isolated after Insulin administration. Mass Spec identifications assigned to mouse proteins have been altered to equivalent rat proteins. Q6IN16 is a UniProt Secondary accession number; this had been reassigned to the Primary accession number O35567 (ATIC). robert.faure@crchul.ulaval.ca Accepted 2015-MAY-20 by ORCHARD Boutchueng-Djidjou M Molecular & cellular proteomics : MCP 2015 Only protein-protein interactions imex curation imex curation I have added the Beta chain feature ID. I am not sure what you want here; I thought we normally do not "do" siRNA results; I have added a comment only (though you might want to reword this) as I would not normally add phosphorylation of any molecule on the basis of results from a non-specific antibody. The Last Enzyme of the De Novo Purine Synthesis Pathway 5-aminoimidazole-4-carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC) Plays a Central Role in Insulin Signaling and the Golgi/Endosomes Protein Network. The Last Enzyme of the De Novo Purine Synthesis Pathway 5-aminoimidazole-4-carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC) Plays a Central Role in Insulin Signaling and the Golgi/Endosomes Protein Network. Molecular & cellular proteomics : MCP 2015 imex curation Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL., Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL. Diabetes - Interactions investigated in the context of Diabetes Checker: please when accepted please send email to Martial.Boutchueng@crchudequebec.ulaval.ca to let him know it has been curated etc...,with the usual request that next time he submits ahead of publication. robert.faure@crchul.ulaval.ca Boutchueng-Djidjou M Only protein-protein interactions imex curation rat-liver rattus norvegicus liver Rat liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL., Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL. Diabetes - Interactions investigated in the context of Diabetes Checker: please when accepted please send email to Martial.Boutchueng@crchudequebec.ulaval.ca to let him know it has been curated etc...,with the usual request that next time he submits ahead of publication. Proteins from the plasma membrane and a Golgi/Endosome fraction were isolated after Insulin administration. robert.faure@crchul.ulaval.ca Accepted 2015-MAY-20 by ORCHARD Boutchueng-Djidjou M Molecular & cellular proteomics : MCP 2015 Only protein-protein interactions imex curation imex curation I have added the Beta chain feature ID. I am not sure what you want here; I thought we normally do not "do" siRNA results; I have added a comment only (though you might want to reword this) as I would not normally add phosphorylation of any molecule on the basis of results from a non-specific antibody. The Last Enzyme of the De Novo Purine Synthesis Pathway 5-aminoimidazole-4-carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC) Plays a Central Role in Insulin Signaling and the Golgi/Endosomes Protein Network. The Last Enzyme of the De Novo Purine Synthesis Pathway 5-aminoimidazole-4-carboxamide Ribonucleotide Formyltransferase/IMP Cyclohydrolase (ATIC) Plays a Central Role in Insulin Signaling and the Golgi/Endosomes Protein Network. Molecular & cellular proteomics : MCP 2015 imex curation Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL., Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL. Diabetes - Interactions investigated in the context of Diabetes Checker: please when accepted please send email to Martial.Boutchueng@crchudequebec.ulaval.ca to let him know it has been curated etc...,with the usual request that next time he submits ahead of publication. robert.faure@crchul.ulaval.ca Boutchueng-Djidjou M Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL., Boutchueng-Djidjou M., Collard-Simard G., Fortier S., Hébert SS., Kelly I., Landry CR., Faure RL. Diabetes - Interactions investigated in the context of Diabetes Checker: please when accepted please send email to Martial.Boutchueng@crchudequebec.ulaval.ca to let him know it has been curated etc...,with the usual request that next time he submits ahead of publication. Proteins from the plasma membrane and a Golgi/Endosome fraction were isolated after Insulin administration. robert.faure@crchul.ulaval.ca Accepted 2015-MAY-20 by ORCHARD Boutchueng-Djidjou M Molecular & cellular proteomics : MCP 2015 Only protein-protein interactions imex curation imex curation I have added the Beta chain feature ID. I am not sure what you want here; I thought we normally do not "do" siRNA results; I have added a comment only (though you might want to reword this) as I would not normally add phosphorylation of any molecule on the basis of results from a non-specific antibody. hacd3_human Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase 3 Butyrate-induced protein 1 PTPLAD1 Protein-tyrosine phosphatase-like A domain-containing protein 1 HACD3 3-hydroxyacyl-CoA dehydratase 3 BIND1 protein protein human Homo sapiens Human MENQVLTPHVYWAQRHRELYLRVELSDVQNPAISITENVLHFKAQGHGAKGDNVYEFHLEFLDLVKPEPVYKLTQRQVNITVQKKVSQWWERLTKQEKRPLFLAPDFDRWLDESDAEMELRAKEEERLNKLRLESEGSPETLTNLRKGYLFMYNLVQFLGFSWIFVNLTVRFCILGKESFYDTFHTVADMMYFCQMLAVVETINAAIGVTTSPVLPSLIQLLGRNFILFIIFGTMEEMQNKAVVFFVFYLWSAIEIFRYSFYMLTCIDMDWKVLTWLRYTLWIPLYPLGCLAEAVSVIQSIPIFNETGRFSFTLPYPVKIKVRFSFFLQIYLIMIFLGLYINFRHLYKQRRRRYGQKKKKIH 1B3591E88DD85D16 grb14_rat Growth factor receptor-bound protein 14 Grb14 GRB14 adapter protein protein protein rat Rattus norvegicus (Rat) Rat MTTSLQDGQSAAGRAGAQDSPLAVQVCRVAQGKGDAQDPAQVPGLHALSPASDATRRGAMDRRKAKDLEVQETPSIPNPFPELCCSPLTSVLSAGLFPRSNSRKKQVIKVYSEDETSRALEVPSDVTARDVCQLLILKNHYVDDNSWTLFEHLSHTGVERTVEDHELLTEVLSHWVMEEDNKLYLRKNYAKYEFFKNPMYFFPEHMVSFATEMNGDRSLTQIPQVFLSSNTYPEIHGFLHAKEQGKKSWKKAYFFLRRSGLYFSTKGTSKEPRHLQFFSEFSTSNVYMSLAGKKKHGAPTPYGFCFKPTKAGGPRDLKMLCAEEDQSRMCWVTAIRLLKYGMQLYQNYMHPSQARSACSSQSVSPMRSVSENSLVAMDFSGQKTRVIDNPTEALSVAVEEGLAWRKKGCLRLGNHGSPTAPSQSSAVNMALHRSQPWFHHRISRDEAQQLITRQGPVDGVFLVRDSQSNPRTFVLSMSHGQKIKHFQIIPVEDDGEVFHTLDDGHTKFTDLIQLVEFYQLNKGVLPCKLKHYCARMAV Interacts with the cytoplasmic domain of the autophosphorylated insulin receptor which is then inhibited. The interaction is mediated by the SH2 domain mint CEBC9037E7868EEF aapk2_human 5'-AMP-activated protein kinase catalytic subunit alpha-2 PRKAA2 AMPK AMPK2 Acetyl-CoA carboxylase kinase Hydroxymethylglutaryl-CoA reductase kinase protein protein human Homo sapiens Human MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR DIP protein P54646 original sequence version: 146 C46AAFC1D5104975 kif5a_human Kinesin heavy chain isoform 5A Neuronal kinesin heavy chain Kinesin heavy chain neuron-specific 1 KIF5A NKHC1 protein protein human Homo sapiens Human MAETNNECSIKVLCRFRPLNQAEILRGDKFIPIFQGDDSVVIGGKPYVFDRVFPPNTTQEQVYHACAMQIVKDVLAGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIARDIFNHIYSMDENLEFHIKVSYFEIYLDKIRDLLDVTKTNLSVHEDKNRVPFVKGCTERFVSSPEEILDVIDEGKSNRHVAVTNMNEHSSRSHSIFLINIKQENMETEQKLSGKLYLVDLAGSEKVSKTGAEGAVLDEAKNINKSLSALGNVISALAEGTKSYVPYRDSKMTRILQDSLGGNCRTTMFICCSPSSYNDAETKSTLMFGQRAKTIKNTASVNLELTAEQWKKKYEKEKEKTKAQKETIAKLEAELSRWRNGENVPETERLAGEEAALGAELCEETPVNDNSSIVVRIAPEERQKYEEEIRRLYKQLDDKDDEINQQSQLIEKLKQQMLDQEELLVSTRGDNEKVQRELSHLQSENDAAKDEVKEVLQALEELAVNYDQKSQEVEEKSQQNQLLVDELSQKVATMLSLESELQRLQEVSGHQRKRIAEVLNGLMKDLSEFSVIVGNGEIKLPVEISGAIEEEFTVARLYISKIKSEVKSVVKRCRQLENLQVECHRKMEVTGRELSSCQLLISQHEAKIRSLTEYMQSVELKKRHLEESYDSLSDELAKLQAQETVHEVALKDKEPDTQDADEVKKALELQMESHREAHHRQLARLRDEINEKQKTIDELKDLNQKLQLELEKLQADYEKLKSEEHEKSTKLQELTFLYERHEQSKQDLKGLEETVARELQTLHNLRKLFVQDVTTRVKKSAEMEPEDSGGIHSQKQKISFLENNLEQLTKVHKQLVRDNADLRCELPKLEKRLRATAERVKALEGALKEAKEGAMKDKRRYQQEVDRIKEAVRYKSSGKRGHSAQIAKPVRPGHYPASSPTNPYGTRSPECISYTNSLFQNYQNLYLQATPSSTSDMYFANSCTSSGATSSGGPLASYQKANMDNGNATDINDNRSDLPCGYEAEDQAKLFPLHQETAAS DIP protein Q12840 original sequence version: 84 C4C6C12342040796 copd_rat Coatomer subunit delta Archain Delta-coat protein Arcn1 Copd protein protein rat Rattus norvegicus (Rat) Rat MVLLAAAVCTKAGKAIVSRQFVEMTRTRIEGLLAAFPKLMNTGKQHTFVETESVRYVYQPMEKLYMVLITTKNSNILEDLETLRLFSRVIPEYCRALEENEISEHCFDLIFAFDEIVALGYRENVNLAQIRTFTEMDSHEEKVFRAVRETQEREAKAEMRRKAKELQQARRDAERQGKKAPGFGGFGSSAVSGGSTAAMITETIIETDKPKVAPAPARPSGPSKALKLGAKGKEVDNFVDKLKSEGETIMSSNMGKRTSEAAKVHAPPINMESVHMKIEEKITLTCGRDGGLQNMELHGMIMLRISDDKFGRIRLHVENEDKKGVQLQTHPNVDKKLFTAESLIGLKNPEKSFPVNSDVGVLKWRLQTTEESFIPLTINCWPSESGNGCDVNIEYELQEDNLELNDVVITIPLPSGVGAPVIGEIDGEYRHDSRRNTLEWCLPVIDAKNKSGSLEFSIPGQPNDFFPVQVSFISKKNYCNIQVTKVTQVDGNSPVRFSTETTFLVDKYEIL 4C25F58A753A7DC8 pur9_human Bifunctional purine biosynthesis protein ATIC ATIC PURH OK/SW-cl.86 AICAR transformylase/inosine monophosphate cyclohydrolase protein protein human Homo sapiens Human MAPGQLALFSVSDKTGLVEFARNLTALGLNLVASGGTAKALRDAGLAVRDVSELTGFPEMLGGRVKTLHPAVHAGILARNIPEDNADMARLDFNLIRVVACNLYPFVKTVASPGVTVEEAVEQIDIGGVTLLRAAAKNHARVTVVCEPEDYVVVSTEMQSSESKDTSLETRRQLALKAFTHTAQYDEAISDYFRKQYSKGVSQMPLRYGMNPHQTPAQLYTLQPKLPITVLNGAPGFINLCDALNAWQLVKELKEALGIPAAASFKHVSPAGAAVGIPLSEDEAKVCMVYDLYKTLTPISAAYARARGADRMSSFGDFVALSDVCDVPTAKIISREVSDGIIAPGYEEEALTILSKKKNGNYCVLQMDQSYKPDENEVRTLFGLHLSQKRNNGVVDKSLFSNVVTKNKDLPESALRDLIVATIAVKYTQSNSVCYAKNGQVIGIGAGQQSRIHCTRLAGDKANYWWLRHHPQVLSMKFKTGVKRAEISNAIDQYVTGTIGEDEDLIKWKALFEEVPELLTEAEKKEWVEKLTEVSISSDAFFPFRDNVDRAKRSGVAYIAAPSGSAADKVVIEACDELGIILAHTNLRLFHH AD778892021F0888 aapk2_rat 5'-AMP-activated protein kinase catalytic subunit alpha-2 Acetyl-CoA carboxylase kinase Hydroxymethylglutaryl-CoA reductase kinase Prkaa2 Ampk Ampk2 protein protein rat Rattus norvegicus (Rat) Rat MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEVEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAQMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSIATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPTGSFMDDMAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSVDSSTAENHSLSGSLTGSLTGSTLSSASPRLGSHTMDFFEMCASLITALAR 2829E07F674D89B1 actb_human Actin, cytoplasmic 1 ACTB Beta-actin protein protein human Homo sapiens Human MDDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF DIP protein P60709 original sequence version: null DIP protein P60709 original sequence version: 63 DIP protein P60709 original sequence version: 128 DIP protein P60709 original sequence version: 129 DIP protein P60709 original sequence version: 58 DIP protein P60709 original sequence version: 66 DIP protein P60709 original sequence version: 88 DIP protein P60709 original sequence version: 89 DIP protein P60709 original sequence version: 86 DIP protein P60709 original sequence version: 138 DIP protein P60709 original sequence version: 112 6AFD05CA94E360E2 p15127-pro_0000016698 Insulin receptor subunit beta Insr protein protein rat Rattus norvegicus (Rat) Rat SLEEVGNVTATTPTLPDFPNISSTIAPTSHEEHRPFEKVVNKESLVISGLRHFTGYRIELQACNQDSPEERSGVAAYVSARTMPEAKADDIVGPVTHEIFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLRGLSPGNYSVRVRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKIIIGPLIFVFLFSVVIGSIYLFLRKRQPDGPMGPLYASSNPEYLSASDVFPSSVYVPDEWEVPREKITLLRELGQGSFGMVYEGNAKDIIKGEVETRVAVKTVNESASLRERIEFLNEASVMKGFTCHHVVRLLGVVSKGQPTLVVMELMAHGDLKSHLRSLRPDAENNPGRPPPTLQEMIQMTAEIADGMAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTASSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDPPDNCPERLTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFYSEENKAPESEELEMEFEDMENVPLDRSSHCQREEAGCREGGSSLSIKRTYDEHIPYTHMNGGKKNGRVLTLPRSNPS 764 1383 80C2AEA17BC62634 pur9_rat Bifunctional purine biosynthesis protein ATIC AICAR transformylase/inosine monophosphate cyclohydrolase Atic Purh protein protein rat Rattus norvegicus (Rat) Rat MASSQLALFSVSDKTGLVEFARNLASLGLSLVASGGTAKAIRDAGLAVRDVSELTGFPEMLGGRVKTLHPAVHAGILARNIPEDAADMARLDFNLIRVVVCNLYPFVKTVASPDVTVEAAVEQIDIGGVTLLRAAAKNHARVTVVCEPEDYGAVAAEMQGSGNKDTSLETRRHLALKAFTHTAQYDEAISDYFRRQYSKGISQMPLRYGMNPHQTPAQLYTLKPKLPITVLNGAPGFINLCDALNAWQLVTELRGAVDIPAAASFKHVSPAGAAVGVPLSEDEARVCMVYDLYPTLTPLAIAYARARGADRMSSFGDFVALSDVCDVPTAKIISREVSDGIVAPGYEEEALKILSKKKNGSYCVLQMDQSYKPDENEVRTLFGLRLSQKRNNGVVDKSLFSNIVTKNKDLPESALRDLIVATIAVKYTQSNSVCYAKDGQVIGIGAGQQSRIHCTRLAGDKANSWWLRHHPRVLSMKFKAGVKRAEVSNAIDQYVTGTIGEGEDLVKWKALFEEVPELLTEAEKKEWVDKLSGVSVSSDAFFPFRDNVDRAKRSGVAYIVAPSGSTADKVVIEACDELGIVLAHTDLRLFHH D9D710794BB12B95 plvap_rat Plasmalemma vesicle-associated protein Plasmalemma vesicle protein 1 gp68 Plvap Pv1 protein protein rat Rattus norvegicus (Rat) Rat MGLSMDRSPYSRTGDRDRGCWYYLRYFFLFVSLIQFLIILGLVLFMIYGNVHATTESSLRATEIRADNLYSQVVGLSAAQANLSKQLNISTLVKDTVMQQLLTTRREVERINASFRQCQGDLITYINYNRFIAAIILSEKQCQEQLKEGNKTCEALLFKLGEKVKTLEMEVVKEKAVCSKDKDSLLAGKRQAEMQQEACGKAREQQKQDQQVTEEQLRKVQSLCLPLDQEKFQADVLNVWRDSLVYRSLDNIGYHYSLMPEFSSLRRTCESLPGIMTTKVEELARGLRAGIERVTRENGELRRQKLELERAIQGEREARTRAGTEAQARETQLRTECARQTQLALEEKAALRTQRDDLERQLEARKRELEQLRTEVDVRISALDTCVKAKSLPAIQPRLPGPPPNPPPIDPASLEEFKKRILESQRPPLVNPAVPPSG 99A016CDD12568C2 d4abi7_rat Very-long-chain (3R)-3-hydroxyacyl-CoA dehydratase Hacd3 LOC100365676 RGD1565496_predicted rCG_58140 protein protein rat Rattus norvegicus (Rat) Rat METQVLTPHVYWAQRHRELYLRVELSDVQNPAISISENVLHFKAQGHGAKGDNVYEFHLEFLDLVKPEPAYRLTQRQVNITVQKKGSHWWERLTKQEKRPLFLAPDFDRWLDESDAEMELRAKEEERLNKLRLESEGSPETLTNLKKGYLFMYNLVQLLGFSWIFVNLTVRFFILGKESFYDTFHTVADMMYFCQMLALVETLNAAIGVTNSPVLPALIQFLGRNFILFLVFGTMEEMQNKAVVFFVFYSWSAIEIFRYPFYMLSCIDMDWKVLTWLRYTMWIPLYPLGCLSEAVAVIQSIPVFNESGRFSFTLPYPLRMKVRFSFFLQVYLVMLFLGLYINFRHLYKQRRRRYGQKKKKLH 5AE8D1EDA94CDB09 p06213-pro_0000016689 Insulin receptor subunit beta INSR protein protein human Homo sapiens Human SLGDVGNVTVAVPTVAAFPNTSSTSVPTSPEEHRPFEKVVNKESLVISGLRHFTGYRIELQACNQDTPEERCSVAAYVSARTMPEAKADDIVGPVTHEIFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLRGLSPGNYSVRIRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKIIIGPLIFVFLFSVVIGSIYLFLRKRQPDGPLGPLYASSNPEYLSASDVFPCSVYVPDEWEVSREKITLLRELGQGSFGMVYEGNARDIIKGEAETRVAVKTVNESASLRERIEFLNEASVMKGFTCHHVVRLLGVVSKGQPTLVVMELMAHGDLKSYLRSLRPEAENNPGRPPPTLQEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMAPESLKDGVFTTSSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDQPDNCPERVTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFHSEENKAPESEELEMEFEDMENVPLDRSSHCQREEAGGRDGGSSLGFKRSYEEHIPYTHMNGGKKNGRILTLPRSNPS 763 1382 3724EBF3F0BAFA39 insr-arcn1-1 1 5 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey association association Table 1 insr-arcn1-2 2 10 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey association association 2E, 3A IR is stated to be auto-phosphorylated as shown by the use of a non-specific anti-phospho-tyrosine antibody (PY20); however no molecular weight markers are shown to confirm the molecular weight (and presumed identity) of the band. In the Plasma Membrane fraction IR phosphorylation was greatest at ~30 seconds post Insulin injection, and then decreased. For the Golgi/Endosome fraction the peak was at 2 minutes post injection and then declined; however ATIC and a protein tyrosine phosphatase (PTP) inhibitor inhibited this decline. insr-atic-1 2 12 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association 3B insr-actb-1 3 16 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey possible_phosphothronine opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain association association 4 B+C Shown here are the results from the IP of the Insulin Receptor using scrambled siRNA. Insulin administration appeared to increase the phosphorylation of the IR as shown by use of a non-specific phosphotyrosine antibody. insr-kif5a-1 3 16 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey possible_phosphothreonine opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt certain certain sequence position certain certain certain sequence position certain association association 4 B Shown here are the results from the IP of the Insulin Receptor using ATIC siRNA. ATIC depletion increased levels of phosphorylated AMPKA and KIF5A detected, but reduced levels of phosphorylated IR (in the presence of Insulin). insr-actb-3 3 16 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey association association 4 C Shown here are the results from the IP of the Insulin Receptor using PTPLAD1 (HACD3) siRNA. Levels of this molecule were decreased, but not absent. Insulin administration appeared to increase the phosphorylation of the IR as shown by use of a non-specific phosphotyrosine antibody.