IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Identification of the amino acids involved in the functional interaction between photosystem I and ferredoxin from Synechocystis sp. PCC 6803 by chemical cross-linking. Identification of the amino acids involved in the functional interaction between photosystem I and ferredoxin from Synechocystis sp. PCC 6803 by chemical cross-linking. J. Biol. Chem. (0021-9258) 1994 imex curation Lelong C., Setif P., Lagoutte B., Bottin H. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Cross-linking of purified Ferredoxin and purified photosytem I. the reaction was catalyzed by N-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in the presence of N-hydroxysulfosuccinimide (NHS). The resulting complexes were analyzed by SDS-PAGE. fig 1 Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Lelong C., Setif P., Lagoutte B., Bottin H. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Cross-linking of purified Ferredoxin and purified photosytem I. the reaction was catalyzed by N-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in the presence of N-hydroxysulfosuccinimide (NHS). The resulting complexes were analyzed by SDS-PAGE. fig 1 Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Biol. Chem. (0021-9258) 1994 Only protein-protein interactions imex curation Identification of the amino acids involved in the functional interaction between photosystem I and ferredoxin from Synechocystis sp. PCC 6803 by chemical cross-linking. Identification of the amino acids involved in the functional interaction between photosystem I and ferredoxin from Synechocystis sp. PCC 6803 by chemical cross-linking. J. Biol. Chem. (0021-9258) 1994 imex curation Lelong C., Setif P., Lagoutte B., Bottin H. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Cross-linking of purified Ferredoxin and purified photosytem I. the reaction was catalyzed by N-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in the presence of N-hydroxysulfosuccinimide (NHS). The resulting complexes were analyzed by SDS-PAGE. fig 1 Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink edman degradation edman degradation Lelong C., Setif P., Lagoutte B., Bottin H. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species Cross-linking of purified Ferredoxin and purified photosytem I. the reaction was catalyzed by N-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) in the presence of N-hydroxysulfosuccinimide (NHS). The resulting complexes were analyzed by SDS-PAGE. fig 1 Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Biol. Chem. (0021-9258) 1994 Only protein-protein interactions imex curation psad_syny3 Photosystem I reaction center subunit II Photosystem I 16 kDa polypeptide psaD slr0737 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MTELSGQPPKFGGSTGGLLSKANREEKYAITWTSASEQVFEMPTGGAAIMNEGENLLYLARKEQCLALGTQLRTKFKPKIQDYKIYRVYPSGEVQYLHPADGVFPEKVNEGREAQGTKTRRIGQNPEPVTIKFSGKAPYEV 263C4B64A5F3F0B7 fer_syny3 Ferredoxin-1 Ferredoxin I petF fed ssl0020 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MASYTVKLITPDGESSIECSDDTYILDAAEEAGLDLPYSCRAGACSTCAGKITAGSVDQSDQSFLDDDQIEAGYVLTCVAYPTSDCTIETHKEEDLY DIP protein P27320 original sequence version: 110 137FFD4F87A66DA1 petf-psad-2 1 3 unspecified role unspecified role neutral component neutral component syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) 4 unspecified role unspecified role neutral component neutral component syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) direct interaction direct interaction Electron paramagnetic resonance measurements have shown that the cross-linked complex between ferredoxin and PSI was entirely photoreducible by photosytem I and that the molar ratio of ferredoxin to PSI was close to 1. petf-psad-3 2 3 unspecified role unspecified role neutral component neutral component lys106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) 4 unspecified role unspecified role neutral component neutral component glu93 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) 12 10 direct interaction direct interaction Electron paramagnetic resonance measurements have shown that the cross-linked complex between ferredoxin and PSI was entirely photoreducible by photosytem I and that the molar ratio of ferredoxin to PSI was close to 1. Chemical cross-linking permitted to demonstrate direct electrostatic attraction between FedI glutamate 93 and lysine 106 of PSI subunit PsaD.