IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Dimerization and inter-chromophore distance of Cph1 phytochrome from Synechocystis, as monitored by fluorescence homo and hetero energy transfer. Dimerization and inter-chromophore distance of Cph1 phytochrome from Synechocystis, as monitored by fluorescence homo and hetero energy transfer. Biochemistry (0006-2960) 2003 imex curation Otto H., Lamparter T., Borucki B., Hughes J., Heyn MP. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species The energy transfer is further analyzed by time-resolved fluorescence intensity and anisotropy decay. fig.2, fig.4, fig.7, fig.8 otto@physik.fu-berlin.de 26-JUL-2004: Contacted by JYOTI Only protein-protein interactions in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Otto H., Lamparter T., Borucki B., Hughes J., Heyn MP. Cyanobacteria - Interaction dataset based on Cyanobacteria proteins and related species The energy transfer is further analyzed by time-resolved fluorescence intensity and anisotropy decay. fig.2, fig.4, fig.7, fig.8 otto@physik.fu-berlin.de Accepted 2006-JUL-18 by DAVET. 26-JUL-2004: Contacted by JYOTI Biochemistry (0006-2960) 2003 Only protein-protein interactions imex curation phy1_syny3 Phytochrome-like protein Cph1 Light-regulated histidine kinase 1 cph1 slr0473 Bacteriophytochrome Cph1 protein protein syny3 Synechocystis sp. (strain PCC 6803 / Kazusa) MATTVQLSDQSLRQLETLAIHTAHLIQPHGLVVVLQEPDLTISQISANCTGILGRSPEDLLGRTLGEVFDSFQIDPIQSRLTAGQISSLNPSKLWARVMGDDFVIFDGVFHRNSDGLLVCELEPAYTSDNLPFLGFYHMANAALNRLRQQANLRDFYDVIVEEVRRMTGFDRVMLYRFDENNHGDVIAEDKRDDMEPYLGLHYPESDIPQPARRLFIHNPIRVIPDVYGVAVPLTPAVNPSTNRAVDLTESILRSAYHCHLTYLKNMGVGASLTISLIKDGHLWGLIACHHQTPKVIPFELRKACEFFGRVVFSNISAQEDTETFDYRVQLAEHEAVLLDKMTTAADFVEGLTNHPDRLLGLTGSQGAAICFGEKLILVGETPDEKAVQYLLQWLENREVQDVFFTSSLSQIYPDAVNFKSVASGLLAIPIARHNFLLWFRPEVLQTVNWGGDPNHAYEATQEDGKIELHPRQSFDLWKEIVRLQSLPWQSVEIQSALALKKAIVNLILRQAEELAQLARNLERSNADLKKFAYIASHDLQEPLNQVSNYVQLLEMRYSEALDEDAKDFIDFAVTGVSLMQTLIDDILTYAKVDTQYAQLTFTDVQEVVDKALANLKQRIEESGAEIEVGSMPAVMADQIQLMQVFQNLIANGIKFAGDKSPKIKIWGDRQEDAWVFAVQDNGIGIDPQFFERIFVIFQRLHTRDEYKGTGMGLAICKKIIEGHQGQIWLESNPGEGSTFYFSIPIGN DIP protein Q55168 original sequence version: 128 A9ECA6D8D5B3C88A cph1-cph1-5 1 2 unspecified role unspecified role fluorescence donor fluorescence donor c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range phycoerythrobilin phycoerythrobilin (PEB) is an analog of the natural chromophore bound to Cph1 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 2 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range phycocyanobilin phycocyanobilin (PCB) is the natural chromophore of Cph1 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Q55168 purified cph1-cph1-6 1 2 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept phycocyanobilin phycocyanobilin (PCB) is the natural chromophore bound to Cph1 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminal binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 2 unspecified role unspecified role fluorescence donor fluorescence donor c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range phycocyanobilin phycocyanobilin (PCB) is the natural chromophore bound to Cph1 tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction The photoactivated form, far-red light absorbing (Pfr), has a very weak dimerisation rate compare to the red light absorbing form (Pr). The distance between the chromophore is estimated to be within the range of 49 and 63 Angstrom. Direct evidence for the existence of a Cph1 monomer/dimer equilibrium was obtained from the increase in anisotropy when PEB/PEB homo dimers were diluted. Kd= 5 +- 3 10e-6M Q55168 purified