IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Impact of temperature on the affinity of SARS-CoV-2 Spike glycoprotein for host ACE2. Impact of temperature on the affinity of SARS-CoV-2 Spike glycoprotein for host ACE2. The Journal of biological chemistry 2021 imex curation Prévost J., Richard J., Gasser R., Ding S., Fage C., Anand SP., Adam D., Vergara NG., Tauzin A., Benlarbi M., Gong SY., Goyette G., Privé A., Moreira S., Charest H., Roger M., Mothes W., Pazgier M., Brochiero E., Boivin G., Abrams CF., Schön A., Finzi A. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus andres.finzi@umontreal.ca Only protein-protein interactions imex curation in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined temperature kelvin kelvin K 283.15 288.15 298.15 308.15 Prévost J., Richard J., Gasser R., Ding S., Fage C., Anand SP., Adam D., Vergara NG., Tauzin A., Benlarbi M., Gong SY., Goyette G., Privé A., Moreira S., Charest H., Roger M., Mothes W., Pazgier M., Brochiero E., Boivin G., Abrams CF., Schön A., Finzi A. Virus - Publications including interactions involving viral proteins Dear checker; I have had "fun" with cloning the Variable Parameters. Please be careful that they are what they should be - I think they are at the moment, but... andres.finzi@umontreal.ca Accepted 2021-SEP-17 AT 15:02 BST AT 15:02 BST by KALPANAP The Journal of biological chemistry 2021 Only protein-protein interactions imex curation imex curation Impact of temperature on the affinity of SARS-CoV-2 Spike glycoprotein for host ACE2. Impact of temperature on the affinity of SARS-CoV-2 Spike glycoprotein for host ACE2. The Journal of biological chemistry 2021 imex curation Prévost J., Richard J., Gasser R., Ding S., Fage C., Anand SP., Adam D., Vergara NG., Tauzin A., Benlarbi M., Gong SY., Goyette G., Privé A., Moreira S., Charest H., Roger M., Mothes W., Pazgier M., Brochiero E., Boivin G., Abrams CF., Schön A., Finzi A. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus andres.finzi@umontreal.ca Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined temperature kelvin kelvin K 283.15 288.15 298.15 308.15 Prévost J., Richard J., Gasser R., Ding S., Fage C., Anand SP., Adam D., Vergara NG., Tauzin A., Benlarbi M., Gong SY., Goyette G., Privé A., Moreira S., Charest H., Roger M., Mothes W., Pazgier M., Brochiero E., Boivin G., Abrams CF., Schön A., Finzi A. Virus - Publications including interactions involving viral proteins andres.finzi@umontreal.ca Accepted 2021-SEP-17 AT 15:02 BST AT 15:02 BST by KALPANAP The Journal of biological chemistry 2021 Only protein-protein interactions imex curation imex curation ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 spike_sars2 Spike glycoprotein S E2 Peplomer protein 2 protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPRRARSVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYIWLGFIAGLIAIVMVTIMLCCMTSCCSCLKGCCSCGSCCKFDEDDSEPVLKGVKLHYT ace2-s-201 6 11 unspecified role unspecified role neutral component neutral component purified purified partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 12 unspecified role unspecified role neutral component neutral component purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 18:34:26 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn501Tyr' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 8 3A and Supp. figure 3A/B/C For WT there was a a 3-fold increase in binding affinity from 43 nM at 35deg.C to 14 nM at 15deg.C. The increase in binding affinity upon a drop in temperature is larger for the N501Y mutant than WT, with the Kd changing from 6.9 nM at 35degC to 1 nM at 15deg.C. ace2-s-203 6 11 unspecified role unspecified role neutral component neutral component purified purified partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 12 unspecified role unspecified role neutral component neutral component purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 7 3A and Supp. figure 3A/B/C For WT there was a a 3-fold increase in binding affinity from 43 nM at 35deg.C to 14 nM at 15deg.C. The increase in binding affinity upon a drop in temperature is larger for the N501Y mutant than WT, with the Kd changing from 6.9 nM at 35degC to 1 nM at 15deg.C. ace2-s-204 6 11 unspecified role unspecified role neutral component neutral component purified purified partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 12 unspecified role unspecified role neutral component neutral component purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 19:06:19 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn501Tyr' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 9 3A and Supp. figure 3A/B/C For WT there was a a 3-fold increase in binding affinity from 43 nM at 35deg.C to 14 nM at 15deg.C. The increase in binding affinity upon a drop in temperature is larger for the N501Y mutant than WT, with the Kd changing from 6.9 nM at 35degC to 1 nM at 15deg.C. ace2-s-205 6 11 unspecified role unspecified role neutral component neutral component purified purified partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 12 unspecified role unspecified role neutral component neutral component purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 19:27:03 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn501Tyr' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 10 3A and Supp. figure 3A/B/C For WT there was a a 3-fold increase in binding affinity from 43 nM at 35deg.C to 14 nM at 15deg.C. The increase in binding affinity upon a drop in temperature is larger for the N501Y mutant than WT, with the Kd changing from 6.9 nM at 35degC to 1 nM at 15deg.C. ace2-s-206 1 11 unspecified role unspecified role prey prey purified purified partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 12 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 19:12:29 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn510Tyr' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 7 3 B/C and Supp. Table 1 Affinity between RBD WT and sACE2 was found to be higher at lower temperatures. ace2-s-207 1 11 unspecified role unspecified role prey prey purified purified partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 12 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 19:34:16 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn501Tyr' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 8 3 B/C and Supp. Table 1 Affinity between RBD WT and sACE2 was found to be higher at lower temperatures. s-ace2-96 1 11 unspecified role unspecified role prey prey purified purified partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 12 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 4 3 B/C and Supp. Table 1 Affinity between RBD WT and sACE2 was found to be higher at lower temperatures. ace2-s-208 1 11 unspecified role unspecified role prey prey purified purified partial ecd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range C-terminus strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f 12 unspecified role unspecified role bait bait purified purified rbd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P0DTC2:p.Asn501Tyr mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain N Y Tue Oct 05 19:30:57 BST 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn501Tyr' human-293f Homo sapiens HEK293F cell line 293f embryonic kidney cell 293f direct interaction direct interaction 10 3 B/C and Supp. Table 1 Affinity between RBD WT and sACE2 was found to be higher at lower temperatures.