IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. SLAS discovery : advancing life sciences R & D 2021 imex curation Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Perveen S., Khalili Yazdi A., Devkota K., Li F., Ghiabi P., Hajian T., Loppnau P., Bolotokova A., Vedadi M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus m.vedadi@utoronto.ca 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column fingerprinting peptide massfingerprinting fingerprinting Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi Virus - Publications including interactions involving viral proteins m.vedadi@utoronto.ca Accepted 2021-JAN-08 AT 11:44 GMT AT 11:44 GMT by ORCHARD Only protein-protein interactions imex curation imex curation A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. SLAS discovery : advancing life sciences R & D 2021 imex curation Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Perveen S., Khalili Yazdi A., Devkota K., Li F., Ghiabi P., Hajian T., Loppnau P., Bolotokova A., Vedadi M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus m.vedadi@utoronto.ca 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi Virus - Publications including interactions involving viral proteins m.vedadi@utoronto.ca Accepted 2021-JAN-08 AT 11:44 GMT AT 11:44 GMT by ORCHARD Only protein-protein interactions imex curation imex curation A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. SLAS discovery : advancing life sciences R & D 2021 imex curation Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Perveen S., Khalili Yazdi A., Devkota K., Li F., Ghiabi P., Hajian T., Loppnau P., Bolotokova A., Vedadi M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus m.vedadi@utoronto.ca 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi Virus - Publications including interactions involving viral proteins m.vedadi@utoronto.ca Accepted 2021-JAN-08 AT 11:44 GMT AT 11:44 GMT by ORCHARD Only protein-protein interactions imex curation imex curation A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. SLAS discovery : advancing life sciences R & D 2021 imex curation Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Perveen S., Khalili Yazdi A., Devkota K., Li F., Ghiabi P., Hajian T., Loppnau P., Bolotokova A., Vedadi M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus m.vedadi@utoronto.ca 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi Virus - Publications including interactions involving viral proteins m.vedadi@utoronto.ca Accepted 2021-JAN-08 AT 11:44 GMT AT 11:44 GMT by ORCHARD Only protein-protein interactions imex curation imex curation A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. A High-Throughput RNA Displacement Assay for Screening SARS-CoV-2 nsp10-nsp16 Complex toward Developing Therapeutics for COVID-19. SLAS discovery : advancing life sciences R & D 2021 imex curation Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Perveen S., Khalili Yazdi A., Devkota K., Li F., Ghiabi P., Hajian T., Loppnau P., Bolotokova A., Vedadi M. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus m.vedadi@utoronto.ca 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi, Sumera Perveen, Aliakbar, Khalili Yazdi, Kanchan Devkota, Fengling Li, Pegah Ghiabi, Taraneh Hajian, Peter Loppnau, Albina Bolotokova, Masoud Vedadi Virus - Publications including interactions involving viral proteins m.vedadi@utoronto.ca Accepted 2021-JAN-08 AT 11:44 GMT AT 11:44 GMT by ORCHARD Only protein-protein interactions imex curation imex curation s-adenosyl-l-homocysteine S-adenosyl-l-homocysteine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p0dtd1-pro_0000449633 2'-O-methyltransferase nsp16 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SSQAWQPGVAMPNLYKMQRMLLEKCDLQNYGDSATLPKGIMMNVAKYTQLCQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDLNDFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEGFFTYICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVNASSSEAFLIGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMSKFPLKLRGTAVMSLKEGQINDMILSLLSKGRLIIRENNRVVISSDVLVNN 6799 7096 n7-megpppg N7-meGpppG small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 7meg5'-ppp5'accccc 7MeG5'-ppp5'ACCCCC small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) sinefungin sinefungin (2S,5S)-2,5-diamino-6-[(2R,3S,R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]hexanoic acid 6,9-Diamino-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9-hexadeoxy-beta-D-ribo-decofuranuronic acid 6,9-Diamino-1-(6-amino-9H-purin-9-yl)-1,5,6,7,8,9-hexadeoxydecofuranuronic acid A 9145 ADENOSYL-ORNITHINE Antibiotic A 9145 sinefungin sinefungina sinefungine sinefunginum (5S)-5-amino-6-[(2R,3S,4R,5R)-5-(6-amino-9H-purin-9-yl)-3,4-dihydroxytetrahydrofuran-2-yl]-L-norleucine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) LMXOHSDXUQEUSF-YECHIGJVSA-N LMXOHSDXUQEUSF-YECHIGJVSA-N s-adenosyl-l-methionine S adenosyl Methionine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) p0dtd1-pro_0000449628 Non-structural protein 10 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV AGNATEVPANSTVLSFCAFAVDAAKAYKDYLASGGQPITNCVKMLCTHTGTGQAITVTPEANMDQESFGGASCCLYCRCHIDHPNPKGFCDLKGKYVQIPTTCANDPVGFTLKNTVCTVCGMWKGYGCSCDQLREPMLQ 4254 4392 n7-methyl-gpppa N7-Methyl-Gpppa small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) nsp10_nsp16_sars2-s_adenosyl_l_methionine-1 5 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 18 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1a nsp10_nsp16_sars2-s_adenosyl_l_homocysteine-1 5 7 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 18 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1b nsp10_nsp16_sars2-sinefungin-1 5 11 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 18 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1c 7meg5'_ppp5'accccc-nsp10_nsp16_sars2-1 3 18 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified fluorescein amidite fluorescein fluorescein label fluorescein c-term range C-terminal range c-term range c-term range C-terminal range c-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figs. 2, 3, S5 Tris(hydroxymethyl)aminomethane [CHEBI:9754], 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) [CHEBI:42334] 7meg5'_ppp5'accccc-n7_megpppg-1 3 9 competitor competitor competitor neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 18 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified fluorescein amidite fluorescein fluorescein label fluorescein c-term range C-terminal range c-term range c-term range C-terminal range c-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figs. 2, 3 Tris(hydroxymethyl)aminomethane [CHEBI:9754], 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) [CHEBI:42334] 7meg5'_ppp5'accccc-n7_methyl_gpppa-1 3 14 competitor competitor competitor neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 18 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified fluorescein amidite fluorescein fluorescein label fluorescein c-term range C-terminal range c-term range c-term range C-terminal range c-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Figs. 2, 3 Tris(hydroxymethyl)aminomethane [CHEBI:9754], 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) [CHEBI:42334] rep-rep-142 6 13 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction S2, S3b rep-rep-143 4 13 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction S3a 7meg5'_ppp5'accccc-7meg5'_ppp5'accccc-1 3 10 competitor competitor competitor neutral component neutral component purified purified c-terminus biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range chemical synthesis Chemical synthesis (Chemical synthesis) 18 unspecified role unspecified role neutral component neutral component purified purified ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component purified purified fluorescein amidite fluorescein fluorescein label fluorescein c-term range C-terminal range c-term range c-term range C-terminal range c-term range chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. S6 Tris(hydroxymethyl)aminomethane [CHEBI:9754], 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) [CHEBI:42334] rep-rep-144 2 13 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction S4 nsp10-nsp16_sars2 SARS-CoV-2 NSP10-NSP16 2'-O-methyltransferase complex NSP10:NSP16 SARS-CoV-2 NSP16/NSP10 complex 13 unspecified role unspecified role nsp16 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 enzyme enzyme nsp10 binding region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 66 68 physical association physical association SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction 2'-O-methyltransferase complex of the SARS-CoV-2 coronavirus which mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. using S-adenosyl-L-methionine (SAM, CHEBI:15414) as the methyl donor. The cap structure is essential for efficient splicing, nuclear export, translation and mRNA stability. Heterodimer Severe acute respiratory syndrome (SARS) [EFO:0000694]: A viral respiratory infection caused by the SARS coronavirus. It is transmitted through close person-to-person contact. It is manifested with high fever, headache, dry cough and myalgias. It may progress to pneumonia and cause death. NSP10 forms a homododecamer in isolation. Accepted 2020-MAR-29 by ORCHARD coronavirus