IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein. Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein. PLoS pathogens 2020 imex curation Dinesh DC., Chalupska D., Silhan J., Koutna E., Nencka R., Veverka V., Boura E. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus veverka@uochb.cas.cz,boura@uochb.cas.cz 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined NaCl mM 150 200 300 500 50 100 Dinesh DC., Chalupska D., Silhan J., Koutna E., Nencka R., Veverka V., Boura E. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus veverka@uochb.cas.cz,boura@uochb.cas.cz Accepted 2021-JAN-24 AT 14:55 GMT AT 14:55 GMT by KALPANAP PLoS pathogens 2020 Only protein-protein interactions imex curation imex curation Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein. Structural basis of RNA recognition by the SARS-CoV-2 nucleocapsid phosphoprotein. PLoS pathogens 2020 imex curation Dinesh DC., Chalupska D., Silhan J., Koutna E., Nencka R., Veverka V., Boura E. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus veverka@uochb.cas.cz,boura@uochb.cas.cz 29-Mar-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro solution nmr solution state nmr solution state nmr solution nmr predetermined predetermined participant predetermined Dinesh DC., Chalupska D., Silhan J., Koutna E., Nencka R., Veverka V., Boura E. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus There are 2 PDBe entries I found in the publication, but the PMID has not been updated as being "published" - so no results are found on searching on the pmid. Please let me know if more can be found. It is unclear if N protein is just 15-N labelled or 13-C/15-N labelled - text says both, above figure 3, but figure 3 legend says only 15-N as does figure S2. Only the 15N version appears to have been used for calculations, so just entered this one. veverka@uochb.cas.cz,boura@uochb.cas.cz Accepted 2021-JAN-24 AT 14:55 GMT AT 14:55 GMT by KALPANAP PLoS pathogens 2020 Only protein-protein interactions imex curation imex curation ssrna_10_mer rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) UCUCUAAACG 7_mer_ssrna rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) CUAAACG ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA 7_mer_dsrna ds rna ds rna double stranded ribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) CACUGAC 10_mer_ssrna rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) UCUCUAAACG 7_mer_dsrna-n-1 8 12 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3A, Supp. figure 2 10_mer_ssrna-n-1 8 13 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3A, Supp. figure 2 n-ssrna_10_mer-1 1 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified n_terminal domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0DTC9:p.Arg92Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R E Sun Dec 13 19:20:09 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC9:p.Arg93Glu' P0DTC9:p.Arg107Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain R E P0DTC9:p.Glu174Arg mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E R P0DTC9:p.Arg68Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R E P0DTC9:p.Ile94Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I A P0DTC9:p.Tyr172Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Sun Dec 13 18:50:11 GMT 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'P0DTC9:p.Tyr 172Ala' P0DTC9:p.Gln163Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q A ecoli Escherichia coli (strain K12) direct interaction direct interaction 4A Binding is strongest in a low salt (50 mM NaCl) buffer (Kd < 1 uM) and very weak (Kd > 400 uM) in high salt buffer (500 mM). The NaCl concentration corresponding to a Kd of 8.3uM is stated to be "physiological". (presumably in the range of ~135-145mM). 7_mer_dsrna-n-2 8 10 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified region 15n label 15n label 15N N15 15N N15 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3A n-ssrna_10_mer-2 1 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified n_terminal domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 6 Supp. figure 4 n-ssrna_10_mer-3 1 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified n_terminal domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 7 Supp. figure 4 n-ssrna_10_mer-4 1 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified n_terminal domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 2 Supp. figure 4 n-ssrna_10_mer-5 1 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified n_terminal domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3 Supp. figure 4 n-ssrna_10_mer-6 1 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified n_terminal domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 4 Supp. figure 4 n-ssrna_10_mer-7 1 9 unspecified role unspecified role neutral component neutral component purified purified chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component purified purified n_terminal domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 5 Supp. figure 4