IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. Biochemical and Biophysical Research Communications 2020 imex curation Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de Accepted 2020-NOV-09 AT 08:34 GMT AT 08:34 GMT by KALPANAP Biochemical and Biophysical Research Communications 2020 Only protein-protein interactions imex curation imex curation High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. Biochemical and Biophysical Research Communications 2020 imex curation Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de Accepted 2020-NOV-09 AT 08:34 GMT AT 08:34 GMT by KALPANAP Biochemical and Biophysical Research Communications 2020 Only protein-protein interactions imex curation imex curation High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. Biochemical and Biophysical Research Communications 2020 imex curation Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. Differential scanning fluorimetry:intrinsic fluorescence during a 20-95 deg. C thermal gradient was measured. zinzula@biochem.mpg.de Accepted 2020-NOV-09 AT 08:34 GMT AT 08:34 GMT by KALPANAP Biochemical and Biophysical Research Communications 2020 Only protein-protein interactions imex curation imex curation High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. Biochemical and Biophysical Research Communications 2020 imex curation Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de Accepted 2020-NOV-09 AT 08:34 GMT AT 08:34 GMT by KALPANAP Biochemical and Biophysical Research Communications 2020 Only protein-protein interactions imex curation imex curation High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. Biochemical and Biophysical Research Communications 2020 imex curation Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation ecoli Escherichia coli (strain K12) x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de Accepted 2020-NOV-09 AT 08:34 GMT AT 08:34 GMT by KALPANAP Biochemical and Biophysical Research Communications 2020 Only protein-protein interactions imex curation imex curation High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. High-resolution structure and biophysical characterization of the nucleocapsid phosphoprotein dimerization domain from the Covid-19 severe acute respiratory syndrome coronavirus 2. Biochemical and Biophysical Research Communications 2020 imex curation Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins Coronavirus - Interactions investigated in the context of Coronavirus Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de 05-Jan-2021: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro 3d-em-single electron microscopy 3D single particle reconstruction 3D-EM-single structure determination identification by structure determination identification by structure determination structure determination Zinzula L., Zinzula L. Virus - Publications including interactions involving viral proteins A preliminary cryo-EM single particle image dataset, resulting in a low-resolution 3D reconstruction. Text states:"we could assign the boundaries of the SARS-CoV-2 N CTD to residues Thr247 and Pro 374 (Fig. 1C and D)". However Figure 1C gives residue 264 as the end of the construct, as do methods and PDBe entries. zinzula@biochem.mpg.de Accepted 2020-NOV-09 AT 08:34 GMT AT 08:34 GMT by KALPANAP Biochemical and Biophysical Research Communications 2020 Only protein-protein interactions imex curation imex curation auuaaag AUUAAAG rna ribonucleic acid RNA rna chemical synthesis Chemical synthesis (Chemical synthesis) AUUAAAG ncap_sars2 Nucleoprotein N Nucleocapsid protein protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA n-71 5 8 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Table 1 n-72 4 8 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Figure 2B n-73 1 8 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Figure 2D n-74 3 8 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Figure 2E Two transitions were observed. Data shows that the SARS-CoV-2 N CTD exists in a stable dimeric state in solution. n-75 2 7 unspecified role unspecified role neutral component neutral component whole molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5-prime cy5 label cy5 label certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ctd sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 4 auuaaag-n-1 6 7 unspecified role unspecified role neutral component neutral component 5-prime cy5 label cy5 label certain certain sequence position certain certain certain sequence position certain whole molecule sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Figure 4