IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2. Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2. Protein science : a publication of the Protein Society 2020 imex curation Kim. Y, Kim Y., Jedrzejczak R., Maltseva NI., Wilamowski M., Endres M., Godzik A., Michalska K., Joachimiak A. Coronavirus - Interactions investigated in the context of Coronavirus doi: https://doi.org/10.1101/2020.03.02.968388 andrzejj@anl.gov 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kim. J Coronavirus - Interactions investigated in the context of Coronavirus doi: https://doi.org/10.1101/2020.03.02.968388 andrzejj@anl.gov Accepted 2020-APR-08 AT 13:32 BST AT 13:32 BST by LIVIAP 2020 Only protein-protein interactions imex curation imex curation Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2. Crystal structure of Nsp15 endoribonuclease NendoU from SARS-CoV-2. Protein science : a publication of the Protein Society 2020 imex curation Kim. Y, Kim Y., Jedrzejczak R., Maltseva NI., Wilamowski M., Endres M., Godzik A., Michalska K., Joachimiak A. Coronavirus - Interactions investigated in the context of Coronavirus doi: https://doi.org/10.1101/2020.03.02.968388 andrzejj@anl.gov 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro ribonuclease assay ribonuclease assay predetermined predetermined participant predetermined Kim. Y, Kim Y., Jedrzejczak R., Maltseva NI., Wilamowski M., Endres M., Godzik A., Michalska K., Joachimiak A. Coronavirus - Interactions investigated in the context of Coronavirus doi: https://doi.org/10.1101/2020.03.02.968388 An increase in fluorescence is detected when the FAM group is cleaved from the TAMRA quenched peptide. andrzejj@anl.gov Accepted 2020-APR-08 AT 13:32 BST AT 13:32 BST by LIVIAP Protein science : a publication of the Protein Society 2020 Only protein-protein interactions imex curation imex curation p0dtd1-pro_0000449632 Uridylate-specific endoribonuclease nsp15 rep ORF1ab polyprotein 1a-1b protein protein SARS-CoV-2 SARS-CoV-2 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 2019-nCoV SLENVAFNVVNKGHFDGQQGEVPVSIINNTVYTKVDGVDVELFENKTTLPVNVAFELWAKRNIKPVPEVKILNNLGVDIAANTVIWDYKRDAPAHISTIGVCSMTDIAKKPTETICAPLTVFFDGRVDGQVDLFRNARNGVLITEGSVKGLQPSVGPKQASLNGVTLIGEAVKTQFNYYKKVDGVVQQLPETYFTQSRNLQEFKPRSQMEIDFLELAMDEFIERYKLEGYAFEHIVYGDFSHSQLGGLHLLIGLAKRFKESPFELEDFIPMDSTVKNYFITDAQTGSSKCVCSVIDLLLDDFVEIIKSQDLSVVSKVVKVTIDYTEISFMLWCKDGHVETFYPKLQ 6453 6798 dna_rna_oligo dArUdAdA nucleic acid nucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) AUAA nsp15_wcpv-1 1 3 unspecified role unspecified role neutral component neutral component purified purified n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 1 dna_rna_oligo-nsp15_wcpv-1 2 4 enzyme target enzyme target substrate substrate neutral component neutral component 5' FAM fluorescein fluorescein label fluorescein certain certain sequence position certain certain certain sequence position certain TAMRA tetramethyl rhodamine label tetramethyl rhodamine label certain certain sequence position certain certain certain sequence position certain 3 enzyme enzyme neutral component neutral component purified purified ecoli Escherichia coli (strain K12) rna cleavage rna cleavage Figure 6 A-C