IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Surface vimentin is critical for the cell entry of SARS-CoV. Surface vimentin is critical for the cell entry of SARS-CoV. Journal of biomedical science 2016 imex curation Yu YT., Chien SC., Chen IY., Lai CT., Tsay YG., Chang SC., Chang MF. Coronavirus - Interactions investigated in the context of Coronavirus scchang093@ntu.edu.tw,mfchang@ntu.edu.tw 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell epithelium Epithelium [epithelial cells] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yu YT., Chien SC., Chen IY., Lai CT., Tsay YG., Chang SC., Chang MF. Coronavirus - Interactions investigated in the context of Coronavirus The virus had been found to be SARS coronavirus TW1 (from back referenced 20484496), whose GenBank id is AY291451.1 for whole genome, AAP37017.1 for Spike glycoprotein, which mapped to P59594 in UniProt scchang093@ntu.edu.tw,mfchang@ntu.edu.tw Accepted 2020-MAR-30 AT 11:39 BST AT 11:39 BST by LIVIAP Journal of biomedical science 2016 Only protein-protein interactions imex curation imex curation Surface vimentin is critical for the cell entry of SARS-CoV. Surface vimentin is critical for the cell entry of SARS-CoV. Journal of biomedical science 2016 imex curation Yu YT., Chien SC., Chen IY., Lai CT., Tsay YG., Chang SC., Chang MF. Coronavirus - Interactions investigated in the context of Coronavirus scchang093@ntu.edu.tw,mfchang@ntu.edu.tw 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Yu YT., Chien SC., Chen IY., Lai CT., Tsay YG., Chang SC., Chang MF. Coronavirus - Interactions investigated in the context of Coronavirus The virus had been found to be SARS coronavirus TW1 (from back referenced 20484496), whose GenBank id is AY291451.1 for whole genome, AAP37017.1 for Spike glycoprotein, which mapped to P59594 in UniProt scchang093@ntu.edu.tw,mfchang@ntu.edu.tw Accepted 2020-MAR-30 AT 11:39 BST AT 11:39 BST by LIVIAP Journal of biomedical science 2016 Only protein-protein interactions imex curation imex curation Surface vimentin is critical for the cell entry of SARS-CoV. Surface vimentin is critical for the cell entry of SARS-CoV. Journal of biomedical science 2016 imex curation Yu YT., Chien SC., Chen IY., Lai CT., Tsay YG., Chang SC., Chang MF. Coronavirus - Interactions investigated in the context of Coronavirus scchang093@ntu.edu.tw,mfchang@ntu.edu.tw 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell epithelium Epithelium [epithelial cells] confocal microscopy confocal microscopy immunostaining immunostaining Yu YT., Chien SC., Chen IY., Lai CT., Tsay YG., Chang SC., Chang MF. Coronavirus - Interactions investigated in the context of Coronavirus scchang093@ntu.edu.tw,mfchang@ntu.edu.tw Accepted 2020-MAR-30 AT 11:39 BST AT 11:39 BST by LIVIAP Journal of biomedical science 2016 Only protein-protein interactions imex curation imex curation Surface vimentin is critical for the cell entry of SARS-CoV. Surface vimentin is critical for the cell entry of SARS-CoV. Journal of biomedical science 2016 imex curation Yu YT., Chien SC., Chen IY., Lai CT., Tsay YG., Chang SC., Chang MF. Coronavirus - Interactions investigated in the context of Coronavirus scchang093@ntu.edu.tw,mfchang@ntu.edu.tw 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell epithelium Epithelium [epithelial cells] crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Yu YT., Chien SC., Chen IY., Lai CT., Tsay YG., Chang SC., Chang MF. Coronavirus - Interactions investigated in the context of Coronavirus Cross-linker: 3,3’-dithiobis(sulfo-succinimidylpropionate) - DTSSP The interacting proteins were immunoprecipitated and analysed by SDS-PAGE and silver staining The virus had been found to be SARS coronavirus TW1 (from back referenced 20484496), whose GenBank id is AY291451.1 for whole genome, AAP37017.1 for Spike glycoprotein which mapped to P59594 in UniProt scchang093@ntu.edu.tw,mfchang@ntu.edu.tw Accepted 2020-MAR-30 AT 11:39 BST AT 11:39 BST by LIVIAP Journal of biomedical science 2016 Only protein-protein interactions imex curation imex curation q1lzx8_chlae Angiotensin-converting enzyme ACE2 protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGEKWSAFLKEQSTLAQMYPLQAIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIHSTGKVCNPNNPQECLLLDPGLNEIMEKSLDYNERLWAWEGWRSEVGKQLRPLYEEYVVLKNEMARANHYKDYGDYWRGDYEVNGVDGYDYNRDQLIEDVERTFEEIKPLYEHLHAYVRAKLMNAYPSYISPTGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVNQAWNAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKVVCHPTAWDLGKGDFRIIMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLLNMLKLGKSEPWTLALENVVGAKNMSVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGANAYKWNDNEMYLFRSSVAYAMRQYFLENKHQTILFGEEDVRVADLKPRISFNFYVTAPKNVSDIIPRTEVEEAIRFSRSRINDAFQLNDNSLEFLGIQSTLVPPYQSPITTWLIVFGVVMAVIVAGIVVLIFTGIRDRKKKNQARSEENPYASIDISKGENNPGFQNTDDVQTSF CF37FF7C7E2C2E2F spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT DIP protein P59594 original sequence version: 48 vime_chlae Vimentin VIM protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey MTTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLTEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESNEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE 2ADE5F232E11EC9F ace2-s-11 4 6 weight silver stain molecular weight estimation by silver staining weight silver stain sequence tag sequence tag identification sequence tag unspecified role unspecified role prey prey engineered engineered infection infection region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 weight silver stain molecular weight estimation by silver staining weight silver stain sequence tag sequence tag identification sequence tag unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level association association Fig. 1 ace2-s-12 1 7 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level 5 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 6 unspecified role unspecified role prey prey engineered engineered infection infection region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 2 s-vim-1 2 7 unspecified role unspecified role bait bait cell lysate cell lysate physiological level physiological level endogenous level endogenous endogenous endogenous level chlae-vero_e6 chlae-vero_e6 cells vero_e6 vero_e6 cell epithelium Epithelium [epithelial cells] 6 unspecified role unspecified role prey prey purified purified c-terminal region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection physical association physical association Fig. 5a s-vim-2 3 7 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell 6 unspecified role unspecified role neutral component neutral component engineered engineered infection infection region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 3