IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. The EMBO journal 2005 imex curation Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. There are different Spike protein sequences for the 3 viral isolates, see:PMID:16670317, figure 1 - gives the differences in the RBD. From Uniprot P59594 entry and NCBI:- "Mutation Asn-479 and Thr-487 in palm civet coronavirus seems necessary and sufficient for the virus to acquire the ability to efficiently infect humans." Uniprot entry P59594 gives the sequence differences between all 3 strains under "Natural variants". [GD strain blast of "S" has only 98.6% homology with P59594, and TOR2 gives 99.9%, but I could not see an alignment ]. PMID: 31996437 in figure 3 gives some of the Civet virus sequence - but there is not an entry for this exact civet in NCBI nor Uniprot - that I can find, so you might have to create one??? (best not to, I think). this is about the 4th/5th publication I have reviewed where you have not entered an email address! Email sent to Livia 9/4/20 -lack of Part.Det. gene accessions for GD and SZ3 spike proteins refers to the same uniprot as MTOR, so I haven't recorded these ones as separate entries. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. There are different Spike protein sequences for the 3 viral isolates, see:PMID:16670317, figure 1 - gives the differences in the RBD. From Uniprot P59594 entry and NCBI:- "Mutation Asn-479 and Thr-487 in palm civet coronavirus seems necessary and sufficient for the virus to acquire the ability to efficiently infect humans." Uniprot entry P59594 gives the sequence differences between all 3 strains under "Natural variants". [GD strain blast of "S" has only 98.6% homology with P59594, and TOR2 gives 99.9%, but I could not see an alignment ]. PMID: 31996437 in figure 3 gives some of the Civet virus sequence - but there is not an entry for this exact civet in NCBI nor Uniprot - that I can find, so you might have to create one??? (best not to, I think). this is about the 4th/5th publication I have reviewed where you have not entered an email address! Email sent to Livia 9/4/20 -lack of Part.Det. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu Accepted 2020-APR-20 AT 09:15 BST AT 09:15 BST by MGT The EMBO journal 2005 Only protein-protein interactions imex curation imex curation Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. The EMBO journal 2005 imex curation Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. There are different Spike protein sequences for the 3 viral isolates, see:PMID:16670317, figure 1 - gives the differences in the RBD. From Uniprot P59594 entry and NCBI:- "Mutation Asn-479 and Thr-487 in palm civet coronavirus seems necessary and sufficient for the virus to acquire the ability to efficiently infect humans." Uniprot entry P59594 gives the sequence differences between all 3 strains under "Natural variants". [GD strain blast of "S" has only 98.6% homology with P59594, and TOR2 gives 99.9%, but I could not see an alignment ]. PMID: 31996437 in figure 3 gives some of the Civet virus sequence - but there is not an entry for this exact civet in NCBI nor Uniprot - that I can find, so you might have to create one??? (best not to, I think). this is about the 4th/5th publication I have reviewed where you have not entered an email address! Email sent to Livia 9/4/20 -lack of Part.Det. gene accessions for GD and SZ3 spike proteins refers to the same uniprot as MTOR, so I haven't recorded these ones as separate entries. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom autoradiography autoradiography Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. There are different Spike protein sequences for the 3 viral isolates, see:PMID:16670317, figure 1 - gives the differences in the RBD. From Uniprot P59594 entry and NCBI:- "Mutation Asn-479 and Thr-487 in palm civet coronavirus seems necessary and sufficient for the virus to acquire the ability to efficiently infect humans." Uniprot entry P59594 gives the sequence differences between all 3 strains under "Natural variants". [GD strain blast of "S" has only 98.6% homology with P59594, and TOR2 gives 99.9%, but I could not see an alignment ]. PMID: 31996437 in figure 3 gives some of the Civet virus sequence - but there is not an entry for this exact civet in NCBI nor Uniprot - that I can find, so you might have to create one??? (best not to, I think). this is about the 4th/5th publication I have reviewed where you have not entered an email address! Email sent to Livia 9/4/20 -lack of Part.Det. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu Accepted 2020-APR-20 AT 09:15 BST AT 09:15 BST by MGT The EMBO journal 2005 Only protein-protein interactions imex curation imex curation Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. The EMBO journal 2005 imex curation Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. There are different Spike protein sequences for the 3 viral isolates, see:PMID:16670317, figure 1 - gives the differences in the RBD. From Uniprot P59594 entry and NCBI:- "Mutation Asn-479 and Thr-487 in palm civet coronavirus seems necessary and sufficient for the virus to acquire the ability to efficiently infect humans." Uniprot entry P59594 gives the sequence differences between all 3 strains under "Natural variants". [GD strain blast of "S" has only 98.6% homology with P59594, and TOR2 gives 99.9%, but I could not see an alignment ]. PMID: 31996437 in figure 3 gives some of the Civet virus sequence - but there is not an entry for this exact civet in NCBI nor Uniprot - that I can find, so you might have to create one??? (best not to, I think). this is about the 4th/5th publication I have reviewed where you have not entered an email address! Email sent to Livia 9/4/20 -lack of Part.Det. gene accessions for GD and SZ3 spike proteins refers to the same uniprot as MTOR, so I haven't recorded these ones as separate entries. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip autoradiography autoradiography Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. There are different Spike protein sequences for the 3 viral isolates, see:PMID:16670317, figure 1 - gives the differences in the RBD. From Uniprot P59594 entry and NCBI:- "Mutation Asn-479 and Thr-487 in palm civet coronavirus seems necessary and sufficient for the virus to acquire the ability to efficiently infect humans." Uniprot entry P59594 gives the sequence differences between all 3 strains under "Natural variants". [GD strain blast of "S" has only 98.6% homology with P59594, and TOR2 gives 99.9%, but I could not see an alignment ]. PMID: 31996437 in figure 3 gives some of the Civet virus sequence - but there is not an entry for this exact civet in NCBI nor Uniprot - that I can find, so you might have to create one??? (best not to, I think). this is about the 4th/5th publication I have reviewed where you have not entered an email address! Email sent to Livia 9/4/20 -lack of Part.Det. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu Accepted 2020-APR-20 AT 09:15 BST AT 09:15 BST by MGT The EMBO journal 2005 Only protein-protein interactions imex curation imex curation Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. The EMBO journal 2005 imex curation Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. There are different Spike protein sequences for the 3 viral isolates, see:PMID:16670317, figure 1 - gives the differences in the RBD. From Uniprot P59594 entry and NCBI:- "Mutation Asn-479 and Thr-487 in palm civet coronavirus seems necessary and sufficient for the virus to acquire the ability to efficiently infect humans." Uniprot entry P59594 gives the sequence differences between all 3 strains under "Natural variants". [GD strain blast of "S" has only 98.6% homology with P59594, and TOR2 gives 99.9%, but I could not see an alignment ]. PMID: 31996437 in figure 3 gives some of the Civet virus sequence - but there is not an entry for this exact civet in NCBI nor Uniprot - that I can find, so you might have to create one??? (best not to, I think). this is about the 4th/5th publication I have reviewed where you have not entered an email address! Email sent to Livia 9/4/20 -lack of Part.Det. gene accessions for GD and SZ3 spike proteins refers to the same uniprot as MTOR, so I haven't recorded these ones as separate entries. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs antibody detection identification by antibody antibody detection Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. There are different Spike protein sequences for the 3 viral isolates, see:PMID:16670317, figure 1 - gives the differences in the RBD. From Uniprot P59594 entry and NCBI:- "Mutation Asn-479 and Thr-487 in palm civet coronavirus seems necessary and sufficient for the virus to acquire the ability to efficiently infect humans." Uniprot entry P59594 gives the sequence differences between all 3 strains under "Natural variants". [GD strain blast of "S" has only 98.6% homology with P59594, and TOR2 gives 99.9%, but I could not see an alignment ]. PMID: 31996437 in figure 3 gives some of the Civet virus sequence - but there is not an entry for this exact civet in NCBI nor Uniprot - that I can find, so you might have to create one??? (best not to, I think). this is about the 4th/5th publication I have reviewed where you have not entered an email address! Email sent to Livia 9/4/20 -lack of Part.Det. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu Accepted 2020-APR-20 AT 09:15 BST AT 09:15 BST by MGT The EMBO journal 2005 Only protein-protein interactions imex curation imex curation Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. The EMBO journal 2005 imex curation Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. There are different Spike protein sequences for the 3 viral isolates, see:PMID:16670317, figure 1 - gives the differences in the RBD. From Uniprot P59594 entry and NCBI:- "Mutation Asn-479 and Thr-487 in palm civet coronavirus seems necessary and sufficient for the virus to acquire the ability to efficiently infect humans." Uniprot entry P59594 gives the sequence differences between all 3 strains under "Natural variants". [GD strain blast of "S" has only 98.6% homology with P59594, and TOR2 gives 99.9%, but I could not see an alignment ]. PMID: 31996437 in figure 3 gives some of the Civet virus sequence - but there is not an entry for this exact civet in NCBI nor Uniprot - that I can find, so you might have to create one??? (best not to, I think). this is about the 4th/5th publication I have reviewed where you have not entered an email address! Email sent to Livia 9/4/20 -lack of Part.Det. gene accessions for GD and SZ3 spike proteins refers to the same uniprot as MTOR, so I haven't recorded these ones as separate entries. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu 04-05-20: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M., Li W., Zhang C., Sui J., Kuhn JH., Moore MJ., Luo S., Wong SK., Huang IC., Xu K., Vasilieva N., Murakami A., He Y., Marasco WA., Guan Y., Choe H., Farzan M. Coronavirus - Interactions investigated in the context of Coronavirus There no entry in NCBI nor Uniprot databases for the SZ3 strain Spike protein isolated from the Palm Civet; thus this protein has not been entered here. There are different Spike protein sequences for the 3 viral isolates, see:PMID:16670317, figure 1 - gives the differences in the RBD. From Uniprot P59594 entry and NCBI:- "Mutation Asn-479 and Thr-487 in palm civet coronavirus seems necessary and sufficient for the virus to acquire the ability to efficiently infect humans." Uniprot entry P59594 gives the sequence differences between all 3 strains under "Natural variants". [GD strain blast of "S" has only 98.6% homology with P59594, and TOR2 gives 99.9%, but I could not see an alignment ]. PMID: 31996437 in figure 3 gives some of the Civet virus sequence - but there is not an entry for this exact civet in NCBI nor Uniprot - that I can find, so you might have to create one??? (best not to, I think). this is about the 4th/5th publication I have reviewed where you have not entered an email address! Email sent to Livia 9/4/20 -lack of Part.Det. Cleavage of the peptide by ACE2 results in increased fluorescence due to the removal of the 2,4-dinitrophenyl moiety that quenches the fluorescence of the 7-methoxycoumarin moiety. Interactions involving chimeric proteins were not captured in accordance to IMEx curation rules. yguan@hkucc.hku.hk,hyeryun.choe@tch.harvard.edu Accepted 2020-APR-20 AT 09:15 BST AT 09:15 BST by MGT The EMBO journal 2005 Only protein-protein interactions imex curation imex curation ace2_rat Angiotensin-converting enzyme 2 Ace2 ACE-related carboxypeptidase protein protein rat Rattus norvegicus (Rat) Rat MSSSCWLLLSLVAVATAQSLIEEKAESFLNKFNQEAEDLSYQSSLASWNYNTNITEENAQKMNEAAAKWSAFYEEQSKIAQNFSLQEIQNATIKRQLKALQQSGSSALSPDKNKQLNTILNTMSTIYSTGKVCNSMNPQECFLLEPGLDEIMATSTDYNRRLWAWEGWRAEVGKQLRPLYEEYVVLKNEMARANNYEDYGDYWRGDYEAEGVEGYNYNRNQLIEDVENTFKEIKPLYEQLHAYVRTKLMEVYPSYISPTGCLPAHLLGDMWGRFWTNLYPLTTPFLQKPNIDVTDAMVNQSWDAERIFKEAEKFFVSVGLPQMTPGFWTNSMLTEPGDDRKVVCHPTAWDLGHGDFRIKMCTKVTMDNFLTAHHEMGHIQYDMAYAKQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLPSNFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFQDKIPREQWTKKWWEMKREIVGVVEPLPHDETYCDPASLFHVSNDYSFIRYYTRTIYQFQFQEALCQAAKHDGPLHKCDISNSTEAGQKLLNMLSLGNSGPWTLALENVVGSRNMDVKPLLNYFQPLFVWLKEQNRNSTVGWSTDWSPYADQSIKVRISLKSALGKNAYEWTDNEMYLFRSSVAYAMREYFSREKNQTVPFGEADVWVSDLKPRVSFNFFVTSPKNVSDIIPRSEVEEAIRMSRGRINDIFGLNDNSLEFLGIYPTLKPPYEPPVTIWLIIFGVVMGTVVVGIVILIVTGIKGRKKKNETKREENPYDSMDIGKGESNAGFQNSDDAQTSF A4079F2407960D28 peptide-mca-yvadapk-dnp 7-methoxycoumarin-YVADAPK(2,4-dinitrophenyl)-OH peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro YVADAPK 9AFE5EFAE96EBC61 q5gdb5_sars Spike glycoprotein S E2 Peplomer protein protein protein SARS coronavirus PC4-145 SARS coronavirus PC4-145 MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFDNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFVVSKPMGTRTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDTWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKRISNCVADYSVLYNSTSFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPAPNCYWPLNGYGFYTTSGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTLIHAEQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSSLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCRQLNRALSGIAAEQDRNTREVFVQVKQMYKTPTLKDFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQEEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT 696C5EA96FB5426F spike_sars Spike glycoprotein S E2 Peplomer protein 2 protein protein sars-cov Human SARS coronavirus SARS-CoV Severe acute respiratory syndrome coronavirus MFIFLLFLTLTSGSDLDRCTTFDDVQAPNYTQHTSSMRGVYYPDEIFRSDTLYLTQDLFLPFYSNVTGFHTINHTFGNPVIPFKDGIYFAATEKSNVVRGWVFGSTMNNKSQSVIIINNSTNVVIRACNFELCDNPFFAVSKPMGTQTHTMIFDNAFNCTFEYISDAFSLDVSEKSGNFKHLREFVFKNKDGFLYVYKGYQPIDVVRDLPSGFNTLKPIFKLPLGINITNFRAILTAFSPAQDIWGTSAAAYFVGYLKPTTFMLKYDENGTITDAVDCSQNPLAELKCSVKSFEIDKGIYQTSNFRVVPSGDVVRFPNITNLCPFGEVFNATKFPSVYAWERKKISNCVADYSVLYNSTFFSTFKCYGVSATKLNDLCFSNVYADSFVVKGDDVRQIAPGQTGVIADYNYKLPDDFMGCVLAWNTRNIDATSTGNYNYKYRYLRHGKLRPFERDISNVPFSPDGKPCTPPALNCYWPLNDYGFYTTTGIGYQPYRVVVLSFELLNAPATVCGPKLSTDLIKNQCVNFNFNGLTGTGVLTPSSKRFQPFQQFGRDVSDFTDSVRDPKTSEILDISPCSFGGVSVITPGTNASSEVAVLYQDVNCTDVSTAIHADQLTPAWRIYSTGNNVFQTQAGCLIGAEHVDTSYECDIPIGAGICASYHTVSLLRSTSQKSIVAYTMSLGADSSIAYSNNTIAIPTNFSISITTEVMPVSMAKTSVDCNMYICGDSTECANLLLQYGSFCTQLNRALSGIAAEQDRNTREVFAQVKQMYKTPTLKYFGGFNFSQILPDPLKPTKRSFIEDLLFNKVTLADAGFMKQYGECLGDINARDLICAQKFNGLTVLPPLLTDDMIAAYTAALVSGTATAGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKQIANQFNKAISQIQESLTTTSTALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDKVEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQAAPHGVVFLHVTYVPSQERNFTTAPAICHEGKAYFPREGVFVFNGTSWFITQRNFFSPQIITTDNTFVSGNCDVVIGIINNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPWYVWLGFIAGLIAIVMVTILLCCMTSCCSCLKGACSCGSCCKFDEDDSEPVLKGVKLHYT DIP protein P59594 original sequence version: 48 ace2_human Angiotensin-converting enzyme 2 ACE2 UNQ868/PRO1885 Angiotensin-converting enzyme homolog Metalloprotease MPROT15 Angiotensin-converting enzyme-related carboxypeptidase protein protein human Homo sapiens Human MSSSSWLLLSLVAVTAAQSTIEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLAQMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQECLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYGDYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIGCLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGLPNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDPASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRLGKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKVRISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRISFNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVSIWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDISKGENNPGFQNTDDVQTSF mint Carboxypeptidase which converts angiotensin I to angiotensin 1-9, a peptide of unknown function, and angiotensin II to angiotensin 1-7, a vasodilator. Also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. May be an important regulator of heart function. In case of human coronaviruses SARS and HCoV-NL63 infections, serve as functional receptor for the spike glycoprotein of both coronaviruses 8EE6EB0A931550E8 ace2_pagla Angiotensin-converting enzyme 2 ACE2 ACE-related carboxypeptidase protein protein Masked palm civet Paguma larvata MSGSFWLLLSFAALTAAQSTTEELAKTFLETFNYEAQELSYQSSVASWNYNTNITDENAKNMNEAGAKWSAYYEEQSKLAQTYPLAEIQDAKIKRQLQALQQSGSSVLSADKSQRLNTILNAMSTIYSTGKACNPNNPQECLLLEPGLDNIMENSKDYNERLWAWEGWRAEVGKQLRPLYEEYVALKNEMARANNYEDYGDYWRGDYEEEWTGGYNYSRNQLIQDVEDTFEQIKPLYQHLHAYVRAKLMDTYPSRISRTGCLPAHLLGDMWGRFWTNLYPLTVPFGQKPNIDVTDAMVNQNWDARRIFKEAEKFFVSVGLPNMTQGFWENSMLTEPGDGRKVVCHPTAWDLGKGDFRIKMCTKVTMDDFLTAHHEMGHIQYDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPNHLKTIGLLSPAFSEDNETEINFLLKQALTIVGTLPFTYMLEKWRWMVFKGAIPKEQWMQKWWEMKRNIVGVVEPVPHDETYCDPASLFHVANDYSFIRYYTRTIYQFQFQEALCQIAKHEGPLHKCDISNSTEAGKKLLEMLSLGRSEPWTLALERVVGAKNMNVTPLLNYFEPLFTWLKEQNRNSFVGWDTDWRPYSDQSIKVRISLKSALGEKAYEWNDNEMYLFRSSIAYAMREYFSKVKNQTIPFVEDNVWVSDLKPRISFNFFVTFSNNVSDVIPRSEVEDAIRMSRSRINDAFRLDDNSLEFLGIEPTLSPPYRPPVTIWLIVFGVVMGAIVVGIVLLIVSGIRNRRKNDQAGSEENPYASVDLNKGENNPGFQHADDVQTSF CF6406851F73E378 spike-ace2-1 2 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9BYF1:p.Lys353His mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K H Tue Jun 30 17:23:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys353His' Q9BYF1:p.Pro84Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain P S Tue Jun 30 15:57:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro84Ser' Q9BYF1:p.Glu110Pro mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E P Tue Jun 30 16:47:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu110Pro' Q9BYF1:p.Pro135_Asp136delinsSerMet mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain PD SM Tue Apr 28 10:32:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro135_Ser136delinsSerMet' Tue Jun 30 19:37:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro135_Asp136delinsSerMet' Q9BYF1:p.Glu160Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E R Tue Jun 30 18:31:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu160Arg' Q9BYF1:p.[His228Asn;Glu231Lys] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H N certain certain sequence position certain certain certain sequence position certain E K Tue Jun 30 15:46:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His228Asn;Glu231Lys]' Q9BYF1:p.His239Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain H Q Tue Apr 28 11:11:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His236Gln' Tue Jun 30 17:36:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His239Gln' Q9BYF1:p.Arg306Glu mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R E Tue Jun 30 18:59:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg306Glu' Q9BYF1:p.[Asn322Gln;Gln325Pro;Glu329Thr] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N Q certain certain sequence position certain certain certain sequence position certain Q P certain certain sequence position certain certain certain sequence position certain E T Tue Jun 30 18:56:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn322Gln;Gln325Pro;Glu329Thr]' Q9BYF1:p.Asn338_Gln340delinsAspAspArg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain NVQ DDR Tue Apr 28 10:49:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn338_Gln340delinsAspAspArg' Tue Jun 30 19:51:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn338_Gln340delinsAspAspArg' Q9BYF1:p.Leu359Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L K Tue Jun 30 17:52:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu359Lys' Q9BYF1:p.Ser425_Asp427delinsProSerAsn mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain SPD PSN Tue Jun 30 18:24:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser425_Asp427delinsProSerAsn' Q9BYF1:p.Lys465_Glu467delinsGlnAspLys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain KGE QDK Tue Jun 30 19:37:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys465_Glu467delinsGlnAspLys' Q9BYF1:p.Arg393Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 18:51:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg393Ala' Q9BYF1:p.Lys31Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K D Tue Jun 30 18:11:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys31Asp' c9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9BYF1:p.[Gln24Lys;Lys26Glu] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain Q K Tue Jun 30 16:04:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln24Lys;Lys26Glu]' Q9BYF1:p.Met82_Pro84delinsAsnPheSer mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain MYP NFS Tue Jun 30 17:59:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met82_Pro84delinsAsnPheSer' Q9BYF1:p.Arg559Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R S Tue Jun 30 19:26:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg559Ser' Q9BYF1:p.[Arg559Ser;Lys562Asn;Glu564Gly] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R S certain certain sequence position certain certain certain sequence position certain K N certain certain sequence position certain certain certain sequence position certain E G Tue Jun 30 15:35:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Arg559Ser;Lys562Asn;Glu564Gly]' Q9BYF1:p.Phe603Thr mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F T Tue Apr 28 11:00:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe603Thr' Tue Jun 30 16:22:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe603Thr' Q9BYF1:p.Lys353Asp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K D Tue Jun 30 19:14:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys353Asp' Q9BYF1:p.Lys353Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:10:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys353Ala' myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9BYF1:p.Tyr41Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Y A Tue Jun 30 20:23:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr41Ala' Q9BYF1:p.Asp355Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 17:04:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp355Ala' Q9BYF1:p.Arg357Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain R A Tue Jun 30 17:24:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg357Ala' Q9BYF1:p.Lys68Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K D Tue Jun 30 16:07:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys68Asp' Q9BYF1:p.Met383Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain M A Tue Jun 30 17:34:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met383Ala' Q9BYF1:p.Pro389Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain P A Tue Jun 30 15:42:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro389Ala' Q9BYF1:p.Glu37Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 16:16:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu37Ala' Q9BYF1:p.Asp38Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 19:12:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp38Ala' Q9BYF1:p.Lys309Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:34:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys309Ala' Q9BYF1:p.Glu312Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E A Tue Jun 30 19:16:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu312Ala' Q9BYF1:p.Thr324Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T A Tue Jun 30 18:31:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr324Ala' Q9BYF1:p.Asp350Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain D A Tue Jun 30 18:08:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp350Ala' Q9BYF1:p.Leu359Ala mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain L A Tue Jun 30 19:23:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu359Ala' Q9BYF1:p.Arg192Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R D Tue Jun 30 18:30:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg192Asp' Q9BYF1:p.Arg219Asp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain R D Tue Jun 30 16:04:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg219Asp' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role bait bait purified purified human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined S1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 1, Fig. 2A-B, Fig. 3A, Fig.4B s-ace2-6 2 9 unspecified role unspecified role bait bait purified purified human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined S1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate c9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5EGZ1:p.[Asn82_Ser84delinsMetTyrPro;His353Lys] mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain H K certain certain sequence position certain certain certain sequence position certain NFS MYP Tue Apr 28 11:09:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His353Lys;Asn82_Ser84delinsMetTyrPro]' Tue Jun 30 17:31:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn82_Ser84delinsMetTyrPro;His353Lys]' myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 2A-B s-ace2-7 4 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9BYF1:p.Lys353His mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K H Tue Jun 30 16:54:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys353His' Q9BYF1:p.Met82_Pro84delinsAsnPheSer mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain MYP NFS Tue Jun 30 19:59:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met82_Pro84delinsAsnPheSer' Q9BYF1:p.Lys353Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K D Tue Jun 30 19:47:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys353Asp' Q9BYF1:p.Lys353Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K A Tue Jun 30 17:02:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys353Ala' myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9BYF1:p.[Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer;Asn90_Val93delinsAspAlaLysIle] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain NLTV DAKI certain certain sequence position certain certain certain sequence position certain DKFNHEAEDLF ETFNYEAQELS Tue Jun 30 17:54:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer;Asn90_Val93delinsAspAlaLysIle]' Q9BYF1:p.Asn90_Val93delinsAspAlaLysIle mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain NLTV DAKI Tue Jun 30 18:42:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn90_Val93delinsAspAlaLysIle' Q9BYF1:p.Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain DKFNHEAEDLF ETFNYEAQELS Tue Jun 30 20:10:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined S1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 2A, Fig. 7A Figure 7A shows that the S1 domains of GD and SZ3 bound substantially more efficiently when either of these regions was introduced into human ACE2, and that introduction of both regions resulted in receptor binding comparable to that with wild-type palm-civet ACE2. s-ace2-8 4 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined S1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 6 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate Q5EGZ1:p.Asn82_Ser84delinsMetTyrPro mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain NFS MYP Tue Jun 30 16:49:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn82_Ser84delinsMetTyrPro' C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q5EGZ1:p.His353Lys mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain H K Tue Jun 30 18:23:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His353Lys' 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5EGZ1:p.[Asn82_Ser84delinsMetTyrPro;His353Lys] mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain H K certain certain sequence position certain certain certain sequence position certain NFS MYP Tue Apr 28 11:20:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[His353Lys;Asn82_Ser84delinsMetTyrPro]' Tue Jun 30 19:01:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn82_Ser84delinsMetTyrPro;His353Lys]' myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 2A ace2-peptide-1 5 10 enzyme enzyme neutral component neutral component purified purified ecd_region sufficient to bind sufficient binding region sufficient to bind range range range range 7 enzyme target enzyme target substrate substrate neutral component neutral component purified purified region dnp dnp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region fluorophore fluorophore undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) cleavage cleavage reaction cleavage Fig. 4A MLN-4760-an ACE2 inhibitor. s-ace2-11 3 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate P59594:p.Lys344Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Tue Jun 30 16:37:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys344Arg' P59594:p.Phe360Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F S Tue Jun 30 20:22:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe360Ser' human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P59594:p.Asn479Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N K Tue Jun 30 16:31:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn479Lys' P59594:p.Thr487Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T S Tue Jun 30 17:35:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr487Ser' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 5B, Fig. 6B ace2-spike-1 1 10 unspecified role unspecified role prey prey purified purified ecd sufficient to bind sufficient binding region sufficient to bind range range range range 9 unspecified role unspecified role bait bait purified purified RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human IgG fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P59594:p.Asn479Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N K Tue Jun 30 17:36:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn479Lys' P59594:p.Lys344Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Tue Jun 30 15:46:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys344Arg' P59594:p.Phe360Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F S Tue Jun 30 18:20:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe360Ser' P59594:p.Thr487Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T S Tue Jun 30 19:02:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr487Ser' direct interaction direct interaction Table 1, Fig. S2 s-ace2-12 3 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate in vitro translated in vitro translated protein in vitro translated C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate P59594:p.Lys344Arg mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K R Tue Jun 30 17:30:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys344Arg' P59594:p.Phe360Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain F S Tue Jun 30 20:03:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe360Ser' human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P59594:p.Asn479Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N K Tue Jun 30 17:16:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn479Lys' P59594:p.Thr487Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T S Tue Jun 30 18:44:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr487Ser' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 5B, Fig. 6B Also SZ3 (genebank AY304486) binds to civet ACE2 ace2-s-19 4 9 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined S1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 11 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate c9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 7A ace2-s-20 3 10 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed cell lysate cell lysate C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9BYF1:p.[Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer;Asn90_Val93delinsAspAlaLysIle] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain NLTV DAKI certain certain sequence position certain certain certain sequence position certain DKFNHEAEDLF ETFNYEAQELS Tue Jun 30 20:14:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer;Asn90_Val93delinsAspAlaLysIle]' Q9BYF1:p.Asn90_Val93delinsAspAlaLysIle mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain NLTV DAKI Tue Jun 30 18:48:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn90_Val93delinsAspAlaLysIle' Q9BYF1:p.Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain DKFNHEAEDLF ETFNYEAQELS Tue Jun 30 17:39:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed cell lysate cell lysate P59594:p.Asn479Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N K Tue Jun 30 18:36:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn479Lys' P59594:p.[Asn479Lys;Thr487Ser] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N K certain certain sequence position certain certain certain sequence position certain T S Tue Jun 30 19:43:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn479Lys;Thr487Ser]' P59594:p.Thr487Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain T S Tue Jun 30 20:10:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr487Ser' human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 7C mutant effects were determined in respect to WT binding partner. ace2-s-21 3 9 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed cell lysate cell lysate P59594:p.Asn479Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain N K Tue Jun 30 19:32:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn479Lys' P59594:p.[Asn479Lys;Thr487Ser] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain N K certain certain sequence position certain certain certain sequence position certain T S Tue Jun 30 18:30:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asn479Lys;Thr487Ser]' P59594:p.Thr487Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T S Tue Jun 30 20:06:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr487Ser' human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 11 unspecified role unspecified role unspecified role unspecified role over-expressed over expressed level over-expressed cell lysate cell lysate c9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 7C ace2-dgspike-1 4 10 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9BYF1:p.[Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer;Asn90_Val93delinsAspAlaLysIle] mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain NLTV DAKI certain certain sequence position certain certain certain sequence position certain DKFNHEAEDLF ETFNYEAQELS Tue Jun 30 16:11:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer;Asn90_Val93delinsAspAlaLysIle]' Q9BYF1:p.Asn90_Val93delinsAspAlaLysIle mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain NLTV DAKI Tue Jun 30 16:47:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn90_Val93delinsAspAlaLysIle' Q9BYF1:p.Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain DKFNHEAEDLF ETFNYEAQELS Tue Jun 30 19:51:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp30_Phe40delinsGluThrPheAsnTyrGluAlaGlnGluLeuSer' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined S1 sufficient to bind sufficient binding region sufficient to bind range range range range Q5GDB5:p.Asn78Asp mutation mutation certain certain sequence position certain certain certain sequence position certain N D Tue Jun 30 20:08:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn78Asp' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 7A Figure 7A shows that the S1 domains of GD and SZ3 bound substantially more efficiently when either of these regions was introduced into human ACE2, and that introduction of both regions resulted in receptor binding comparable to that with wild-type palm-civet ACE2. s-ace2-17 3 11 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate in vitro translated in vitro translated protein in vitro translated C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q5GDB5:p.Asn78Asp mutation mutation certain certain sequence position certain certain certain sequence position certain N D Tue Jun 30 15:41:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn78Asp' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 5B Also SZ3 (genebank AY304486) binds to civet ACE2 s-ace2-18 3 10 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed cell lysate cell lysate in vitro translated in vitro translated protein in vitro translated C9 tag tag tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 35s radiolabel 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 8 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed cell lysate cell lysate human IgG1 tag fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined RBD domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q5GDB5:p.Asn78Asp mutation mutation certain certain sequence position certain certain certain sequence position certain N D Tue Jun 30 16:17:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn78Asp' human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 5B Also SZ3 (genebank AY304486) binds to civet ACE2 weak interaction