IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Function of TAF(II)-containing complex without TBP in transcription by RNA polymerase II. Function of TAF(II)-containing complex without TBP in transcription by RNA polymerase II. Nature (0028-0836) 1998 Wieczorek E., Brand M., Jacq X., Tora L. Chromatin - Epigenetic interactions resulting in chromatin modulation The TBP free TAFII complex (TFTC) was purified as follows: Nuclear extract was subject to SSDNA cellulose chromatography followed by anti-TAFII30 Immunoprecipitation; anti-TBP immunoprecipitations were carried out on the eluate to remove the TBP. laszlo@igbmc.u-strasbg.fr,laszlo@titus.u-strasbg.fr 27-Aug-2008: Contacted by JYOTI. davet human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wieczorek E., Brand M., Jacq X., Tora L. Chromatin - Epigenetic interactions resulting in chromatin modulation The TBP free TAFII complex (TFTC) was purified as follows: Nuclear extract was subject to SSDNA cellulose chromatography followed by anti-TAFII30 Immunoprecipitation; anti-TBP immunoprecipitations were carried out on the eluate to remove the TBP. laszlo@igbmc.u-strasbg.fr,laszlo@titus.u-strasbg.fr Accepted 2008-AUG-10 by DAVET. Nature (0028-0836) 1998 davet Function of TAF(II)-containing complex without TBP in transcription by RNA polymerase II. Function of TAF(II)-containing complex without TBP in transcription by RNA polymerase II. Nature (0028-0836) 1998 Wieczorek E., Brand M., Jacq X., Tora L. Chromatin - Epigenetic interactions resulting in chromatin modulation The TBP free TAFII complex (TFTC) was purified as follows: Nuclear extract was subject to SSDNA cellulose chromatography followed by anti-TAFII30 Immunoprecipitation; anti-TBP immunoprecipitations were carried out on the eluate to remove the TBP. laszlo@igbmc.u-strasbg.fr,laszlo@titus.u-strasbg.fr 27-Aug-2008: Contacted by JYOTI. davet human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wieczorek E., Brand M., Jacq X., Tora L. Chromatin - Epigenetic interactions resulting in chromatin modulation The TBP free TAFII complex (TFTC) was purified as follows: Nuclear extract was subject to SSDNA cellulose chromatography followed by anti-TAFII30 Immunoprecipitation; anti-TBP immunoprecipitations were carried out on the eluate to remove the TBP. laszlo@igbmc.u-strasbg.fr,laszlo@titus.u-strasbg.fr Accepted 2008-AUG-10 by DAVET. 27-Aug-2008: Contacted by JYOTI. Nature (0028-0836) 1998 davet Function of TAF(II)-containing complex without TBP in transcription by RNA polymerase II. Function of TAF(II)-containing complex without TBP in transcription by RNA polymerase II. Nature (0028-0836) 1998 Wieczorek E., Brand M., Jacq X., Tora L. Chromatin - Epigenetic interactions resulting in chromatin modulation The TBP free TAFII complex (TFTC) was purified as follows: Nuclear extract was subject to SSDNA cellulose chromatography followed by anti-TAFII30 Immunoprecipitation; anti-TBP immunoprecipitations were carried out on the eluate to remove the TBP. laszlo@igbmc.u-strasbg.fr,laszlo@titus.u-strasbg.fr 27-Aug-2008: Contacted by JYOTI. davet human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wieczorek E., Brand M., Jacq X., Tora L. Chromatin - Epigenetic interactions resulting in chromatin modulation The TBP free TAFII complex (TFTC) was purified as follows: Nuclear extract was subject to SSDNA cellulose chromatography followed by anti-TAFII30 Immunoprecipitation; anti-TBP immunoprecipitations were carried out on the eluate to remove the TBP. laszlo@igbmc.u-strasbg.fr,laszlo@titus.u-strasbg.fr Accepted 2008-AUG-10 by DAVET. 27-Aug-2008: Contacted by JYOTI. Nature (0028-0836) 1998 davet taf4_human Transcription initiation factor TFIID subunit 4 TAF4 TAF2C TAF2C1 TAF4A TAFII130 TAFII135 TBP-associated factor 4 Transcription initiation factor TFIID 135 kDa subunit Transcription initiation factor TFIID 130 kDa subunit RNA polymerase II TBP-associated factor subunit C protein protein human Homo sapiens Human MAAGSDLLDEVFFNSEVDEKVVSDLVGSLESQLAASAAHHHHLAPRTPEVRAAAAGALGNHVVSGSPAGAAGAGPAAPAEGAPGAAPEPPPAGRARPGGGGPQRPGPPSPRRPLVPAGPAPPAAKLRPPPEGSAGSCAPVPAAAAVAAGPEPAPAGPAKPAGPAALAARAGPGPGPGPGPGPGPGPGKPAGPGAAQTLNGSAALLNSHHAAAPAVSLVNNGPAALLPLPKPAAPGTVIQTPPFVGAAAPPAPAAPSPPAAPAPAAPAAAPPPPPPAPATLARPPGHPAGPPTAAPAVPPPAAAQNGGSAGAAPAPAPAAGGPAGVSGQPGPGAAAAAPAPGVKAESPKRVVQAAPPAAQTLAASGPASTAASMVIGPTMQGALPSPAAVPPPAPGTPTGLPKGAAGAVTQSLSRTPTATTSGIRATLTPTVLAPRLPQPPQNPTNIQNFQLPPGMVLVRSENGQLLMIPQQALAQMQAQAHAQPQTTMAPRPATPTSAPPVQISTVQAPGTPIIARQVTPTTIIKQVSQAQTTVQPSATLQRSPGVQPQLVLGGAAQTASLGTATAVQTGTPQRTVPGATTTSSAATETMENVKKCKNFLSTLIKLASSGKQSTETAANVKELVQNLLDGKIEAEDFTSRLYRELNSSPQPYLVPFLKRSLPALRQLTPDSAAFIQQSQQQPPPPTSQATTALTAVVLSSSVQRTAGKTAATVTSALQPPVLSLTQPTQVGVGKQGQPTPLVIQQPPKPGALIRPPQVTLTQTPMVALRQPHNRIMLTTPQQIQLNPLQPVPVVKPAVLPGTKALSAVSAQAAAAQKNKLKEPGGGSFRDDDDINDVASMAGVNLSEESARILATNSELVGTLTRSCKDETFLLQAPLQRRILEIGKKHGITELHPDVVSYVSHATQQRLQNLVEKISETAQQKNFSYKDDDRYEQASDVRAQLKFFEQLDQIEKQRKDEQEREILMRAAKSRSRQEDPEQLRLKQKAKEMQQQELAQMRQRDANLTALAAIGPRKKRKVDCPGPGSGAEGSGPGSVVPGSSGVGTPRQFTRQRITRVNLRDLIFCLENERETSHSLLLYKAFLK DIP protein O00268 original sequence version: 153 DIP protein O00268 original sequence version: 139 BC2F5B5F143DB145 tbp_human TATA-box-binding protein TBP TF2D TFIID GTF2D1 TATA-box factor TATA-binding factor TATA sequence-binding protein Transcription initiation factor TFIID TBP subunit protein protein human Homo sapiens Human MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT DIP protein P20226 original sequence version: 106 DIP protein P20226 original sequence version: 157 DIP protein P20226 original sequence version: 173 DIP protein P20226 original sequence version: 194 DIP protein P20226 original sequence version: 179 DIP protein P20226 original sequence version: 131 A61A578D972B970B taf5_human Transcription initiation factor TFIID subunit 5 Transcription initiation factor TFIID 100 kDa subunit TAF5 TAF2D protein protein human Homo sapiens Human MAALAEEQTEVAVKLEPEGPPTLLPPQAGDGAGEGSGGTTNNGPNGGGGNVAASSSTGGDGGTPKPTVAVSAAAPAGAAPVPAAAPDAGAPHDRQTLLAVLQFLRQSKLREAEEALRREAGLLEEAVAGSGAPGEVDSAGAEVTSALLSRVTASAPGPAAPDPPGTGASGATVVSGSASGPAAPGKVGSVAVEDQPDVSAVLSAYNQQGDPTMYEEYYSGLKHFIECSLDCHRAELSQLFYPLFVHMYLELVYNQHENEAKSFFEKFHGDQECYYQDDLRVLSSLTKKEHMKGNETMLDFRTSKFVLRISRDSYQLLKRHLQEKQNNQIWNIVQEHLYIDIFDGMPRSKQQIDAMVGSLAGEAKREANKSKVFFGLLKEPEIEVPLDDEDEEGENEEGKPKKKKPKKDSIGSKSKKQDPNAPPQNRIPLPELKDSDKLDKIMNMKETTKRVRLGPDCLPSICFYTFLNAYQGLTAVDVTDDSSLIAGGFADSTVRVWSVTPKKLRSVKQASDLSLIDKESDDVLERIMDEKTASELKILYGHSGPVYGASFSPDRNYLLSSSEDGTVRLWSLQTFTCLVGYKGHNYPVWDTQFSPYGYYFVSGGHDRVARLWATDHYQPLRIFAGHLADVNCTRFHPNSNYVATGSADRTVRLWDVLNGNCVRIFTGHKGPIHSLTFSPNGRFLATGATDGRVLLWDIGHGLMVGELKGHTDTVCSLRFSRDGEILASGSMDNTVRLWDAIKAFEDLETDDFTTATGHINLPENSQELLLGTYMTKSTPVVHLHFTRRNLVLAAGAYSPQ DIP protein Q15542 original sequence version: 141 DIP protein Q15542 original sequence version: 62 DIP protein Q15542 original sequence version: 131 DIP protein Q15542 original sequence version: 152 87A178BFB7071992 taf1_human Transcription initiation factor TFIID subunit 1 TAF1 BA2R CCG1 TAF2A CCGS Transcription initiation factor TFIID 250 kDa subunit TBP-associated factor 250 kDa Cell cycle gene 1 protein protein protein human Homo sapiens Human MGPGCDLLLRTAATITAAAIMSDTDSDEDSAGGGPFSLAGFLFGNINGAGQLEGESVLDDECKKHLAGLGALGLGSLITELTANEELTGTDGALVNDEGWVRSTEDAVDYSDINEVAEDESRRYQQTMGSLQPLCHSDYDEDDYDADCEDIDCKLMPPPPPPPGPMKKDKDQDSITGEKVDFSSSSDSESEMGPQEATQAESEDGKLTLPLAGIMQHDATKLLPSVTELFPEFRPGKVLRFLRLFGPGKNVPSVWRSARRKRKKKHRELIQEEQIQEVECSVESEVSQKSLWNYDYAPPPPPEQCLSDDEITMMAPVESKFSQSTGDIDKVTDTKPRVAEWRYGPARLWYDMLGVPEDGSGFDYGFKLRKTEHEPVIKSRMIEEFRKLEENNGTDLLADENFLMVTQLHWEDDIIWDGEDVKHKGTKPQRASLAGWLPSSMTRNAMAYNVQQGFAATLDDDKPWYSIFPIDNEDLVYGRWEDNIIWDAQAMPRLLEPPVLTLDPNDENLILEIPDEKEEATSNSPSKESKKESSLKKSRILLGKTGVIKEEPQQNMSQPEVKDPWNLSNDEYYYPKQQGLRGTFGGNIIQHSIPAVELRQPFFPTHMGPIKLRQFHRPPLKKYSFGALSQPGPHSVQPLLKHIKKKAKMREQERQASGGGEMFFMRTPQDLTGKDGDLILAEYSEENGPLMMQVGMATKIKNYYKRKPGKDPGAPDCKYGETVYCHTSPFLGSLHPGQLLQAFENNLFRAPIYLHKMPETDFLIIRTRQGYYIRELVDIFVVGQQCPLFEVPGPNSKRANTHIRDFLQVFIYRLFWKSKDRPRRIRMEDIKKAFPSHSESSIRKRLKLCADFKRTGMDSNWWVLKSDFRLPTEEEIRAMVSPEQCCAYYSMIAAEQRLKDAGYGEKSFFAPEEENEEDFQMKIDDEVRTAPWNTTRAFIAAMKGKCLLEVTGVADPTGCGEGFSYVKIPNKPTQQKDDKEPQPVKKTVTGTDADLRRLSLKNAKQLLRKFGVPEEEIKKLSRWEVIDVVRTMSTEQARSGEGPMSKFARGSRFSVAEHQERYKEECQRIFDLQNKVLSSTEVLSTDTDSSSAEDSDFEEMGKNIENMLQNKKTSSQLSREREEQERKELQRMLLAAGSAASGNNHRDDDTASVTSLNSSATGRCLKIYRTFRDEEGKEYVRCETVRKPAVIDAYVRIRTTKDEEFIRKFALFDEQHREEMRKERRRIQEQLRRLKRNQEKEKLKGPPEKKPKKMKERPDLKLKCGACGAIGHMRTNKFCPLYYQTNAPPSNPVAMTEEQEEELEKTVIHNDNEELIKVEGTKIVLGKQLIESADEVRRKSLVLKFPKQQLPPKKKRRVGTTVHCDYLNRPHKSIHRRRTDPMVTLSSILESIINDMRDLPNTYPFHTPVNAKVVKDYYKIITRPMDLQTLRENVRKRLYPSREEFREHLELIVKNSATYNGPKHSLTQISQSMLDLCDEKLKEKEDKLARLEKAINPLLDDDDQVAFSFILDNIVTQKMMAVPDSWPFHHPVNKKFVPDYYKVIVNPMDLETIRKNISKHKYQSRESFLDDVNLILANSVKYNGPESQYTKTAQEIVNVCYQTLTEYDEHLTQLEKDICTAKEAALEEAELESLDPMTPGPYTPQPPDLYDTNTSLSMSRDASVFQDESNMSVLDIPSATPEKQVTQEGEDGDGDLADEEEGTVQQPQASVLYEDLLMSEGEDDEEDAGSDEEGDNPFSAIQLSESGSDSDVGSGGIRPKQPRMLQENTRMDMENEESMMSYEGDGGEASHGLEDSNISYGSYEEPDPKSNTQDTSFSSIGGYEVSEEEEDEEEEEQRSGPSVLSQVHLSEDEEDSEDFHSIAGDSDLDSDE DIP protein P21675 original sequence version: 92 DIP protein P21675 original sequence version: 149 DIP protein P21675 original sequence version: 172 93BE3D181A72ABEB taf10_human Transcription initiation factor TFIID subunit 10 Transcription initiation factor TFIID 30 kDa subunit STAF28 TAF10 TAF2H TAFII30 TAF2A protein protein human Homo sapiens Human MSCSGSGADPEAAPASAASAPGPAPPVSAPAALPSSTAAENKASPAGTAGGPGAGAAAGGTGPLAARAGEPAERRGAAPVSAGGAAPPEGAISNGVYVLPSAANGDVKPVVSSTPLVDFLMQLEDYTPTIPDAVTGYYLNRAGFEASDPRIIRLISLAAQKFISDIANDALQHCKMKGTASGSSRSKSKDRKYTLTMEDLTPALSEYGINVKKPHYFT DIP protein Q12962 original sequence version: 59 032ED5CA97EBE411 taf6_human Transcription initiation factor TFIID subunit 6 Transcription initiation factor TFIID 70 kDa subunit Transcription initiation factor TFIID 80 kDa subunit RNA polymerase II TBP-associated factor subunit E TAF6 TAF2E TAFII70 protein protein human Homo sapiens Human MAEEKKLKLSNTVLPSESMKVVAESMGIAQIQEETCQLLTDEVSYRIKEIAQDALKFMHMGKRQKLTTSDIDYALKLKNVEPLYGFHAQEFIPFRFASGGGRELYFYEEKEVDLSDIINTPLPRVPLDVCLKAHWLSIEGCQPAIPENPPPAPKEQQKAEATEPLKSAKPGQEEDGPLKGKGQGATTADGKGKEKKAPPLLEGAPLRLKPRSIHELSVEQQLYYKEITEACVGSCEAKRAEALQSIATDPGLYQMLPRFSTFISEGVRVNVVQNNLALLIYLMRMVKALMDNPTLYLEKYVHELIPAVMTCIVSRQLCLRPDVDNHWALRDFAARLVAQICKHFSTTTNNIQSRITKTFTKSWVDEKTPWTTRYGSIAGLAELGHDVIKTLILPRLQQEGERIRSVLDGPVLSNIDRIGADHVQSLLLKHCAPVLAKLRPPPDNQDAYRAEFGSLGPLLCSQVVKARAQAALQAQQVNRTTLTITQPRPTLTLSQAPQPGPRTPGLLKVPGSIALPVQTLVSARAAAPPQPSPPPTKFIVMSSSSSAPSTQQVLSLSTSAPGSGSTTTSPVTTTVPSVQPIVKLVSTATTAPPSTAPSGPGSVQKYIVVSLPPTGEGKGGPTSHPSPVPPPASSPSPLSGSALCGGKQEAGDSPPPAPGTPKANGSQPNSGSPQPAP DIP protein P49848 original sequence version: 66 06C6BFA563580823 taf11_human Transcription initiation factor TFIID subunit 11 TAF11 TAF2I PRO2134 Transcription initiation factor TFIID 28 kDa subunit TFIID subunit p30-beta protein protein human Homo sapiens Human MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELESQDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNRYEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMPPLQPKHMREAVRRLKSKGQIPNSKHKKIIFF DIP protein Q15544 original sequence version: 124 77C7BDC667D19A8F taf12_human Transcription initiation factor TFIID subunit 12 TAF12 TAF15 TAF2J TAFII20 Transcription initiation factor TFIID 20/15 kDa subunits protein protein human Homo sapiens Human MNQFGPSALINLSNFSSIKPEPASTPPQGSMANSTAVVKIPGTPGAGGRLSPENNQVLTKKKLQDLVREVDPNEQLDEDVEEMLLQIADDFIESVVTAACQLARHRKSSTLEVKDVQLHLERQWNMWIPGFGSEEIRPYKKACTTEAHKQRMALIRKTTKK 054B017CD7BF99CE taf7_human Transcription initiation factor TFIID subunit 7 Transcription initiation factor TFIID 55 kDa subunit RNA polymerase II TBP-associated factor subunit F TAF7 TAF2F TAFII55 protein protein human Homo sapiens Human MSKSKDDAPHELESQFILRLPPEYASTVRRAVQSGHVNLKDRLTIELHPDGRHGIVRVDRVPLASKLVDLPCVMESLKTIDKKTFYKTADICQMLVSTVDGDLYPPVEEPVASTDPKASKKKDKDKEKKFIWNHGITLPLKNVRKRRFRKTAKKKYIESPDVEKEVKRLLSTDAEAVSTRWEIIAEDETKEAENQGLDISSPGMSGHRQGHDSLEHDELREIFNDLSSSSEDEDETQHQDEEDINIIDTEEDLERQLQDKLNESDEQHQENEGTNQLVMGIQKQIDNMKGKLQETQDRAKRQEDLIMKVENLALKNRFQAVLDELKQKEDREKEQLSSLQEELESLLEK DIP protein Q15545 original sequence version: 127 DIP protein Q15545 original sequence version: 53 DIP protein Q15545 original sequence version: 71 DIP protein Q15545 original sequence version: 113 0AAFD3E33E3CCD6E taf5l-trrap 1 12 unspecified role unspecified role unspecified role unspecified role 11 unspecified role unspecified role unspecified role unspecified role 9 unspecified role unspecified role unspecified role unspecified role 8 unspecified role unspecified role unspecified role unspecified role 4 unspecified role unspecified role unspecified role unspecified role 6 unspecified role unspecified role unspecified role unspecified role association association 1C Although TBP was completely depleted from the input fraction by two rounds of anti-TBP immunoprecipitations (see the lack of TBP in supernatants 2 and 3 in Fig. 1a, c), surprisingly a portion of hTAFII30 and several other TAFIIs, such as hTAFII135, hTAFII100, hTAFII80, hTAFII55 and hTAFII20, remained in the supernatant fraction, as detected by immunoblotting (see supernatant 3; Fig. 1c) Many TAFIIs, including hTAFII20, hTAFII30, hTAFII55, hTAFII80, hTAFII100 and hTAFII135, are apparently common to both TFTC and TFIIDbeta. In contrast, hTAFII250 and hTAFII28, which strongly interact with TBP, were absent from the TFTC fraction (Fig. 1c). Moreover, recombinant TAFIIs (hTAFII18, hTAFII20, hTAFII31, hTAFII80, hTAFII100 and hTAFII135) can assemble into a stable complex, containing stoichiometric amounts of each of these TAFIIs. tfiid-taf150-1 3 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait association association 1C Many TAFIIs, including hTAFII20, hTAFII30, hTAFII55, hTAFII80, hTAFII100 and hTAFII135, are apparently common to both TFTC and TFIIDbeta. In contrast, hTAFII250 and hTAFII28, which strongly interact with TBP, were absent from the TFTC fraction (Fig. 1c). Moreover, recombinant TAFIIs (hTAFII18, hTAFII20, hTAFII31, hTAFII80, hTAFII100 and hTAFII135) can assemble into a stable complex, containing stoichiometric amounts of each of these TAFIIs. tfiid-tbp 2 8 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey association association 1B