IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. Cell (0092-8674) 2007 imex curation Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells treated with 1% formaldehyde, protein complexes separated by sucrose density gradient centrifugation, by anti-p53 pulldown and by SDS-PAGE prior to mass spectrometry. clp3@columbia.edu 22-Jan-2008: Contacted by JYOTI. DAVET Only protein-protein interactions 5 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. crosslink cross-linking study crosslink ms participant Identification by mass spectrometry ms participant Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells treated with 1% formaldehyde, protein complexes separated by sucrose density gradient centrifugation, by anti-p53 pulldown and by SDS-PAGE prior to mass spectrometry. clp3@columbia.edu Accepted 2007-DEC-22 by DAVET. Cell (0092-8674) 2007 DAVET Only protein-protein interactions 5 imex curation hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. Cell (0092-8674) 2007 imex curation Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells treated with 1% formaldehyde, protein complexes separated by sucrose density gradient centrifugation, by anti-p53 pulldown and by SDS-PAGE prior to mass spectrometry. clp3@columbia.edu 22-Jan-2008: Contacted by JYOTI. DAVET Only protein-protein interactions 5 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells treated with 1% formaldehyde, protein complexes in some cases separated by sucrose density gradient centrifugation prior to anti-p53 coimmunoprecipitation. clp3@columbia.edu Accepted 2007-DEC-22 by DAVET. 22-Jan-2008: Contacted by JYOTI. Cell (0092-8674) 2007 DAVET Only protein-protein interactions 5 imex curation hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. Cell (0092-8674) 2007 imex curation Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells treated with 1% formaldehyde, protein complexes separated by sucrose density gradient centrifugation, by anti-p53 pulldown and by SDS-PAGE prior to mass spectrometry. clp3@columbia.edu 22-Jan-2008: Contacted by JYOTI. DAVET Only protein-protein interactions 5 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis clp3@columbia.edu Accepted 2007-DEC-22 by DAVET. 22-Jan-2008: Contacted by JYOTI. Cell (0092-8674) 2007 DAVET Only protein-protein interactions 5 imex curation hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. Cell (0092-8674) 2007 imex curation Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells treated with 1% formaldehyde, protein complexes separated by sucrose density gradient centrifugation, by anti-p53 pulldown and by SDS-PAGE prior to mass spectrometry. clp3@columbia.edu 22-Jan-2008: Contacted by JYOTI. DAVET Only protein-protein interactions 5 human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells treated with 1% formaldehyde, chromatin solution then cleared, and protein bound DNA isolated by immobilized anti-bait. clp3@columbia.edu Accepted 2007-DEC-22 by DAVET. 22-Jan-2008: Contacted by JYOTI. Cell (0092-8674) 2007 DAVET Only protein-protein interactions 5 imex curation hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. Cell (0092-8674) 2007 imex curation Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells treated with 1% formaldehyde, protein complexes separated by sucrose density gradient centrifugation, by anti-p53 pulldown and by SDS-PAGE prior to mass spectrometry. clp3@columbia.edu 22-Jan-2008: Contacted by JYOTI. DAVET Only protein-protein interactions 5 human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells treated with 1% formaldehyde, complexes purified by anti-p53 coimmunoprecipitation clp3@columbia.edu Accepted 2007-DEC-22 by DAVET. 22-Jan-2008: Contacted by JYOTI. Cell (0092-8674) 2007 DAVET Only protein-protein interactions 5 imex curation hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. Cell (0092-8674) 2007 imex curation Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells treated with 1% formaldehyde, protein complexes separated by sucrose density gradient centrifugation, by anti-p53 pulldown and by SDS-PAGE prior to mass spectrometry. clp3@columbia.edu 22-Jan-2008: Contacted by JYOTI. DAVET Only protein-protein interactions 5 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells treated with 1% formaldehyde, chromatin solution then cleared, and protein bound DNA isolated by immobilized anti-bait. clp3@columbia.edu Accepted 2007-DEC-22 by DAVET. 22-Jan-2008: Contacted by JYOTI. Cell (0092-8674) 2007 DAVET Only protein-protein interactions 5 imex curation hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. hCAS/CSE1L associates with chromatin and regulates expression of select p53 target genes. Cell (0092-8674) 2007 imex curation Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis Chromatin - Epigenetic interactions resulting in chromatin modulation Cells treated with 1% formaldehyde, protein complexes separated by sucrose density gradient centrifugation, by anti-p53 pulldown and by SDS-PAGE prior to mass spectrometry. clp3@columbia.edu 22-Jan-2008: Contacted by JYOTI. DAVET Only protein-protein interactions 5 human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tanaka T., Ohkubo S., Tatsuno I., Prives C. Apoptosis - Interactions involving proteins with a function related to apoptosis clp3@columbia.edu Accepted 2007-DEC-22 by DAVET. 22-Jan-2008: Contacted by JYOTI. Cell (0092-8674) 2007 DAVET Only protein-protein interactions 5 imex curation p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 xpo2_human Exportin-2 Importin-alpha re-exporter Chromosome segregation 1-like protein Cellular apoptosis susceptibility protein CSE1L CAS XPO2 protein protein human Homo sapiens Human MELSDANLQTLTEYLKKTLDPDPAIRRPAEKFLESVEGNQNYPLLLLTLLEKSQDNVIKVCASVTFKNYIKRNWRIVEDEPNKICEADRVAIKANIVHLMLSSPEQIQKQLSDAISIIGREDFPQKWPDLLTEMVNRFQSGDFHVINGVLRTAHSLFKRYRHEFKSNELWTEIKLVLDAFALPLTNLFKATIELCSTHANDASALRILFSSLILISKLFYSLNFQDLPEFFEDNMETWMNNFHTLLTLDNKLLQTDDEEEAGLLELLKSQICDNAALYAQKYDEEFQRYLPRFVTAIWNLLVTTGQEVKYDLLVSNAIQFLASVCERPHYKNLFEDQNTLTSICEKVIVPNMEFRAADEEAFEDNSEEYIRRDLEGSDIDTRRRAACDLVRGLCKFFEGPVTGIFSGYVNSMLQEYAKNPSVNWKHKDAAIYLVTSLASKAQTQKHGITQANELVNLTEFFVNHILPDLKSANVNEFPVLKADGIKYIMIFRNQVPKEHLLVSIPLLINHLQAESIVVHTYAAHALERLFTMRGPNNATLFTAAEIAPFVEILLTNLFKALTLPGSSENEYIMKAIMRSFSLLQEAIIPYIPTLITQLTQKLLAVSKNPSKPHFNHYMFEAICLSIRITCKANPAAVVNFEEALFLVFTEILQNDVQEFIPYVFQVMSLLLETHKNDIPSSYMALFPHLLQPVLWERTGNIPALVRLLQAFLERGSNTIASAAADKIPGLLGVFQKLIASKANDHQGFYLLNSIIEHMPPESVDQYRKQIFILLFQRLQNSKTTKFIKSFLVFINLYCIKYGALALQEIFDGIQPKMFGMVLEKIIIPEIQKVSGNVEKKICAVGITKLLTECPPMMDTEYTKLWTPLLQSLIGLFELPEDDTIPDEEHFIDIEDTPGYQTAFSQLAFAGKKEHDPVGQMVNNPKIHLAQSLHKLSTACPGRVPSMVSTSLNAEALQYLQGYLQAASVTLL DIP protein P55060 original sequence version: 121 08E837AB5008EBFD rir2b_human_gene Human RRM2B gene gene gene human Homo sapiens Human mdm2_human_gene Human MDM2 gene gene gene human Homo sapiens Human bbc3_human_gene Human BBC3 gene gene gene human Homo sapiens Human tpip1_human_gene Human TP53AIP1 gene gene gene human Homo sapiens Human tp53i3_human_gene Human TP53I3 gene gene gene human Homo sapiens Human cdkn1a_human_gene Human CDKN1A gene gene gene human Homo sapiens Human tp53-cse1l-1 1 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey direct interaction direct interaction Isolation and identification of hCAS/CSE1L polypeptide from p53-associated rapidly sedimenting crosslinked protein complexes are described in Experimental Procedures and Figure S1 Figures 1E and S1 tp53-tp53i3_gene-1 6 14 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait association association TP53 binds to the gene promoter region ChIP analysis showed p53 association with p21 and HDM2 promoters in both intermediately and rapidly sedimenting gradient fractions, while p53 at PIG3 and p53AIP1 promoters was exclusively in the most rapidly sedimenting fractions (Figure 1C). Figure 1C tp53-cdkn1a_gene-1 6 8 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey association association TP53 binds to the gene promoter region ChIP analysis showed p53 association with p21 and HDM2 promoters in both intermediately and rapidly sedimenting gradient fractions, while p53 at PIG3 and p53AIP1 promoters was exclusively in the most rapidly sedimenting fractions (Figure 1C). Figure 1C tp53-mdm2_gene-1 6 8 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey association association TP53 binds to the gene promoter region ChIP analysis showed p53 association with p21 and HDM2 promoters in both intermediately and rapidly sedimenting gradient fractions, while p53 at PIG3 and p53AIP1 promoters was exclusively in the most rapidly sedimenting fractions (Figure 1C). Figure 1C tp53-tp53aip_gene-1 6 8 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey association association TP53 binds to the gene promoter region ChIP analysis showed p53 association with p21 and HDM2 promoters in both intermediately and rapidly sedimenting gradient fractions, while p53 at PIG3 and p53AIP1 promoters was exclusively in the most rapidly sedimenting fractions (Figure 1C). Figure 1C tp53-cse1l-2 2 8 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey direct interaction direct interaction hCAS/CSE1L protein could be detected in immunoprecipitates of Ad-p53 from extracts of crosslinked H1299 cells, and a weak signal above background was even detected in uncrosslinked cell lysates (Figure 2A). Figures 2A, 2B When crosslinked sonicated cell extracts were subjected to sucrose gradient sedimentation, CSE1L coimmunoprecipitated with p53 in the rapidly sedimenting fraction), but not in the intermediate sedimenting fraction tp53-cse1l-3 7 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Endogenous hCAS/CSE1L was detectable within anti-p53 immunoprecipitates, and this was markedly augmented when p53 levels were increased following DNA damage (Figure 2C). Figures 2C Adriamycin - DNA damaging agent. tp53-cse1l-4 7 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Reciprocally, p53 could be detected in immunoprecipitates of hCAS/CSE1L in lysates of either H1299 cells expressing ectopic p53 (Figure 2D) or MCF-7 cells expressing endogenous p53 after adriamycin treatment (Figure 2E). Figures 2E Adriamycin - DNA damaging agent. tp53-cse1l-5 3 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Reciprocally, p53 could be detected in immunoprecipitates of hCAS/CSE1L in lysates of either H1299 cells expressing ectopic p53 (Figure 2D) or MCF-7 cells expressing endogenous p53 after adriamycin treatment (Figure 2E). Figures 2D Adriamycin - DNA damaging agent. cse1l-tp53i3_gene-2 5 9 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role prey prey association association CSE1L binds to the gene promoter region When anti-FLAG antibody (M2) was used to measure association of ectopic wild-type hCAS/CSE1L with the p53 binding site at −318 within the PIG3 promoter, there was a strong signal over the vector control (Figure 4C, lower panels). Figure 4C tp53-bbc3_gene-1 6 8 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey association association TP53 binds to the gene promoter region Figure 4C tp53-rrm2b_gene-1 6 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait association association TP53 binds to the gene promoter region Figure 4E cse1l-tp53i3_gene-1 6 9 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey association association CSE1L binds to the gene promoter region in both the presence and absence of p53 Thus, there was a striking correlation between binding of hCAS/CSE1L uniquely to those promoters of p53 target genes (PIG3, p53AIP1, and p53R2) whose induction was reduced after hCAS/CSE1L downregulation. Figure 4D cse1l-tp53aip-1 6 13 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association CSE1L binds to the gene promoter region in both the presence and absence of p53 Thus, there was a striking correlation between binding of hCAS/CSE1L uniquely to those promoters of p53 target genes (PIG3, p53AIP1, and p53R2) whose induction was reduced after hCAS/CSE1L downregulation. Figure 4D cse1l-rrm2b-1 6 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association CSE1L binds to the gene promoter region in both the presence and absence of p53 Thus, there was a striking correlation between binding of hCAS/CSE1L uniquely to those promoters of p53 target genes (PIG3, p53AIP1, and p53R2) whose induction was reduced after hCAS/CSE1L downregulation. Figure 4D tp53-tp53i3_gene-2 4 14 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait association association TP53 binds to the gene promoter region Figure 4E tp53-tp53aip_gene-2 4 8 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey association association TP53 binds to the gene promoter region Figure 4E tp53-rrm2b_gene-2 4 8 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association TP53 binds to the gene promoter region Figure 4E tp53-cdkn1a_gene-2 4 15 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait association association TP53 binds to the gene promoter region Figure 4E tp53-bbc3_gene-2 4 12 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait association association TP53 binds to the gene promoter region Figure 4E cse1l-tp53aip-2 4 9 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey association association CSE1L binds to the gene promoter region in both the presence and absence of p53 Figure 4E cse1l-rrm2b-2 4 9 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association CSE1L binds to the gene promoter region in both the presence and absence of p53 Figure 4E cse1l-tp53i3_gene-3 4 14 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association CSE1L binds to the gene promoter region in both the presence and absence of p53 Figure 4E