IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor. JHDM2A, a JmjC-containing H3K9 demethylase, facilitates transcription activation by androgen receptor. Cell (0092-8674) 2006 imex curation Yamane K., Toumazou C., Tsukada Y., Erdjument-Bromage H., Tempst P., Wong J., Zhang Y. Chromatin - Epigenetic interactions resulting in chromatin modulation Where the origins of the proteins were difficult to ascertain the experiments and corresponding interactions have not been entered. yi_zhang@med.unc.edu 21-Dec-2006: Contacted by JYOTI. Only protein-protein interactions 1 in vitro In vitro enzymatic study enzymatic study ms participant Identification by mass spectrometry ms participant Yamane K., Toumazou C., Tsukada Y., Erdjument-Bromage H., Tempst P., Wong J., Zhang Y. Chromatin - Epigenetic interactions resulting in chromatin modulation Where the origins of the proteins were difficult to ascertain the experiments and corresponding interactions have not been entered. yi_zhang@med.unc.edu Accepted 2006-DEC-11 by DAVET. 21-Dec-2006: Contacted by JYOTI. Cell (0092-8674) 2006 Only protein-protein interactions 1 imex curation h3_peptide_2-18 AA 2-18 peptide peptide polypeptide oligopeptide oligopeptide polypeptide in vitro In vitro ARTKQTARKSTGGKAPR An amino acid sequence which is not represented in UniProt Conserved n-terminal sequence in mouse, human, arath, encal, chick, brana, eupes, crilo, calmo, cicin, rat, bovin, ictu, schpo, xentr and more corresponds to AA 1-18 of histone H3. FFC910D796787586 kdm2a_human Lysine-specific demethylase 2A CXXC8 [Histone-H3]-lysine-36 demethylase 1A F-box/LRR-repeat protein 11 F-box and leucine-rich repeat protein 11 F-box protein FBL7 F-box protein Lilina CXXC-type zinc finger protein 8 KDM2A FBL7 KIAA1004 JHDM1A FBXL11 JmjC domain-containing histone demethylation protein 1A FBL11 protein protein human Homo sapiens Human MEPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLHTNKYNANFVTFMEGKDFNVEYIQRGGLRDPLIFKNSDGLGIKMPDPDFTVNDVKMCVGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKLYNVISLEFSHTRLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCLMSVRGCYTDFHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSGKQGDIFLGDRVSDCQRIELKQGYTFVIPSGWIHAVYTPTDTLVFGGNFLHSFNIPMQLKIYNIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHLTKEFQKESLSMDLELNGLESGNGDEEAVDREPRRLSSRRSVLTSPVANGVNLDYDGLGKTCRSLPSLKKTLAGDSSSDCSRGSHNGQVWDPQCAPRKDRQVHLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIADVKILLEELANSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPIVSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNEETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSEKAQKRKMEESDEEAVQAKVLRPLRSCDEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATERTMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRHSPRVLVQHCPARTPQRGDEEGLGGEEEEEEEEEEEDDSAEEGGAARLNGRGSWAQDGDESWMQREVWMSVFRYLSRRELCECMRVCKTWYKWCCDKRLWTKIDLSRCKAIVPQALSGIIKRQPVSLDLSWTNISKKQLTWLVNRLPGLKDLLLAGCSWSAVSALSTSSCPLLRTLDLRWAVGIKDPQIRDLLTPPADKPGQDNRSKLRNMTDFRLAGLDITDATLRLIIRHMPLLSRLDLSHCSHLTDQSSNLLTAVGSSTRYSLTELNMAGCNKLTDQTLIYLRRIANVTLIDLRGCKQITRKACEHFISDLSINSLYCLSDEKLIQKIS DIP protein Q9Y2K7 original sequence version: 89 88620A363A5C5842 h3_peptide-fbxl11 1 2 enzyme target enzyme target substrate substrate unspecified role unspecified role lys 9 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain lys 9 n6me1lys N6-methyl-L-lysine (2S)-2-amino-6-methylaminohexanoic acid MOD_RES N6-methyllysine N(zeta)-methyllysine N6-methyl-L-lysine N6-methylated L-lysine epsilon-methyllysine Methyl Methylation N-methyl Lysyl N6Me1Lys certain certain sequence position certain certain certain sequence position certain c terminal lys biotin tag biotin tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range n terminal ala acala N-acetyl-L-alanine (2S)-2-(acetamido)propanoic acid 2-(acetylamino)propanoic acid 2-(acetylazanyl)propanoic acid MOD_RES N-acetylalanine N-acetyl-L-alanine N-acetylated L-alanine acetylalanine AcAla n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro 3 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction 4E, 4F in Figure 4E demonstrate that demethylation of a dimethyl-K9 peptide depends on the presence of JHDM2A and generated products with masses that correlate with both monomethyl and unmethylated forms of the peptide, indicating that both mono- and dimethyl-K9 can serve as substrates. Both mono and dimethylated forms of peptide serve as substrates of JHDM2A