IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The ATPase activity of MCM2-7 is dispensable for pre-RC assembly but is required for DNA unwinding. The ATPase activity of MCM2-7 is dispensable for pre-RC assembly but is required for DNA unwinding. EMBO J. (0261-4189) 2005 Ying CY., Gautier J. Chromatin - Epigenetic interactions resulting in chromatin modulation 1 and 2A jg130@columbia.edu in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ying CY., Gautier J. Chromatin - Epigenetic interactions resulting in chromatin modulation 1 and 2A jg130@columbia.edu EMBO J. (0261-4189) 2005 The ATPase activity of MCM2-7 is dispensable for pre-RC assembly but is required for DNA unwinding. The ATPase activity of MCM2-7 is dispensable for pre-RC assembly but is required for DNA unwinding. EMBO J. (0261-4189) 2005 Ying CY., Gautier J. Chromatin - Epigenetic interactions resulting in chromatin modulation 1 and 2A jg130@columbia.edu in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ying CY., Gautier J. Chromatin - Epigenetic interactions resulting in chromatin modulation 1 and 2A jg130@columbia.edu EMBO J. (0261-4189) 2005 mc6zb_xenla Zygotic DNA replication licensing factor mcm6-B Zygotic minichromosome maintenance protein 6-B zmcm6-b zmcm6b protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDLVDPSQSAAAAAGTQLVKDEVAEKCQKLFQDFLEEFRGSDGELKYQSDAEELIRPERNTLLVSFVDLEQFNQQLATTIQEEFYRVYPYLCRAVKAFARDHGNIPQNKEFYVAFQELPTRHKIRELTTPRIGSLLRISGQVVRTHPVHPELVSGTFLCLDCQTLVRDVEQQFKYTQPSICRNPVCANRKRFMLDTNKSRFVDFQKVRIQETQAELPRGSIPRSVEVILRAEAVESCQAGDRCDFTGSLIVVPDISQLSTPGVRAETSSRVGGREGYEAEGVQGLRALGVRDLSYKLVFLACYVCPTNPRFGGKDLHEEDMTAESIKNQMSVKEWEKVFEMSQDKNLYHNLCTSLFPTVHGNDEVKRGILLMLFGGVPKSTMEGTSLRGDINVCVVGDPSTAKSQFLKHVEEFSPRAVYTSGKASTAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMDTKDQVAIHEAMEQQTISITKAGVKATLNARTSILAAANPVGGRYDRAKSLKQNVNLSAPIMSRFDLFFILVDECNEVTDYAIARRIVDLHSRIEESIDRVYTLDEVRRYLLFARQFKPKISKESEDFIVEQYKRLRQRDGSGVTKSAWRITVRQLESMIRLSEGMARMHCSDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEDEHEVEEPQEGINGDADVPNGVNGHINGINGHAEETNAAPPKPSLRLNFAEYKRISNLLVLQLRKIEDEDDENETSQRKSELINWYLKEIESEIDSEEELVNRKQIIDKVIHRLVHYDQILIELIQTGLKGTEDENVAKEEDPYLVVNPNYILED A96CF39A8BE3FDF4 mcm3m_xenla Maternal DNA replication licensing factor mcm3 Maternal minichromosome maintenance protein 3 P1 homolog p100 XRLF subunit beta mmcm3 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MDYGGGFEDHELREAQREYLDFLDDDQDQGLYHGKVRDMIGSNEHRLIVNLNDVRRKNDKRANLMLNDAFAETIAFQRALKDLVASIDATYAKQFEEFSVGFEGSFGSKHVSPRTLTASLLGSLVCVEGIVTKCSLVRPKVMRSVHYCPATKKTLERKYSDLTSLEAFPSSSIYPTKDEENNPLETEYGLSTYKDHQTLSIQEMPEKAPAGQLPRSVDIIADDDLVDKCKPGDRVQIVGIYRCLPSKQGGFTSGTFRTILLANNIKLMSKEIAPTFSADDVAKIKKFCKAHSKDIFEHLSKSLAPSIHGHEYIKKAILCMLLGGNEKVLENGTRIRGDINVLLIGDPSVAKSQLLRYVLHTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIQARLNARCSVLAAANPVYGRYDQYRTPMENIGLQDSLLSRFDLLFIVLDKMDADNDQEIADHVLRMHRYRTPGEQDGYALPLGCSVEIFATDDPNASDVTDQELQIYEKHDNLLHGPRKNKSKIVSMQFIRKYIHVAKLIKPVLTSEAADYISQEYAKIRNHDQINNDSARTMPVTARALETMIRLSTAHAKVRMSKTIERQDAETALELVQFAYFKKVLAKEKKKTDKDLHDENLSQDTLSQESVRKSSRRAGKIADSQDDSMDPYSFSEQDSSLNENLSQSLRPQRKKAESQDGKRSLSQNRTKEFKAALLKAFKSSRSQSVAVSQLLELINKGNPEPFERSEVKEALDNMQNDNQVMVSEDVVFLI 70B08E9BE0F3A81F mcm5a_xenla DNA replication licensing factor mcm5-A CDC46 homolog A CDC46p p92 mcm5-a cdc46-a protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSGFDDLGVYYSDSFGGEQQVGDDGQAKKSQLKKRFREFLRQYRIGTDRTGFTFKYRDELKRHYNLGEYWIEVEMEDLASFDEDLADYLYKQPTEHLQLLEEAAQEVADEVTRPRPAGEETIQEIQVMLRSDANPANIRSLKSEQMSHLVKIPGIIIAATAVRAKATKISIQCRSCRNTIGNIAVRPGLEGYAMPRKCNTEQAGRPNCPLDPYFIIPDKCKCVDFQTLKLQESPDAVPHGELPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIRKSGKTSTKGRDRVGVGIRSSYIRVVGIQVDTEGTGRSAAGAITPQEEEEFRRLAAKPDIYETVAKSIAPSIYGSSDIKKAIACLLFGGSRKRLPDGLTRRGDVNLLMLGDPGTAKSQLLKFVERCSPIGVYTSGKGSSAAGLTASVMRDPVSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVYGRWDDTKGEENIDFMPTILSRFDMIFIVKDEHNEQRDMTLAKHVMNVHLSARTQSSSVEGEVDLNTLKKYIAYCRAKCGPRLSAEAAEKLKNRYILMRSGAREHERETEKRSSIPITVRQLEAIVRISESLGKMKLQPFATETDVEEALRLFQVSTLDAAMSGSLSGVEGFTTQEDQEMLSRIEKQMKKRFAIGSQVSEHSIIQDFLKQKYPEHAIHKVLSLMMRRGEIQHRLQRKVLYRIK 702BA90C2F510720 mcm2_xenla DNA replication licensing factor mcm2 Minichromosome maintenance protein 2 BM28-homolog p112 mcm2 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MADSSESFNIATSPRAGSRRDALTSSPGRDLPPFEDESEGMFGDGVVPEEEEDGEELIGDAMERDYRPISELDRYEVEGLDDEEDVEDLTASQREAAEQSMRMRDREMGRELGRMRRGLLYDSDEEEEDRPARKRRMAERAAEGAPEEDEEMIESIENLEDMKGHTVREWVSMAATRLEIYHRFKNFLRTHVDEHGHNVFKEKISDMCKENKESLPVNYEDLAAREHVLAYFLPEAPAEMLKIFDEAAKEVVLVMYPKYDRIAREIHVRISHLPLVEELRSLRQLHLNQLIRTSGVVTCCTGVLPQLSMVKYNCNKCNFILGPFFQSQNQEVRPGSCPECQSFGPFEINMEETVYQNYQRITIQESPGKVAAGRLPRSKDAILLADLVDSCKPGDEIELTGIYHNNYDGSLNTANGFPVFATVILANHITKKDDKVAVGELTDEDVKAIVALSKDERIGERIFASIAPSIYGHEDIKRGLALALFGGEAKNPGGKHKVRGDINVLLCGDPGTAKSQFLKYVEKVASRAVFTTGQGASAVGLTAYVQRHPVTKEWTLEAGALVLADRGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTSLQARCTVIAASNPIGGRYDPSLTFSENVDLTEPIVSRFDILCVVRDTVDPVQDEMLARFVVSSHIKHHPSSKDIANGDAAEFALPNTFGVEALPQEVLKKYIMYAKEKIRPKLNQMDQDKVAKMYSDLRKESMATGSIPITVRHIESMIRMAEAHARMHLRDYVVEDDVNMAIRVMLESFIDTQKFSVMRSMRKTFARYLAFRRDNNELLLFVLKQLIAEQVTYQRNRYGAQQDTIEVPEKDLVDKARQINIHNLSAFYDSDLFKMNKFTHDVKKKLIIQQF 524C7B3673E2B2DD mcm4b_xenla DNA replication licensing factor mcm4-B Minichromosome maintenance protein 4-B CDC21 homolog-B P1-CDC21-B mcm4-b protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MSSPTSTPSRRRNKRGRGSNPPTPHGEEVQSPPSQRRRTEDSTSIGELLPMPTSPSGDVQSPSGQELLFSSPAPSRHSAHQSELDLSSPLTYGTPSSRVEGTPRSGIRGTPARQRPDLGSARKVKQVDLHSDQPAAEELVTSEQSLGQKLVIWGTDVNVATCKEKFQRFVQRFIDPSAKEEDNVGLDLNEPIYMQRLEEINVVGDPFLNIDCDHLRNFDQDLYRQLVCYPQEVIPTFDMAANEIFFERYPDSILEHQIQVRPYNALKTRNMRSLNPEDIDQLITISGMVIRTSQIIPEMQEAFFKCQVCAFTTRVEIDRGRIAEPSVCKHCNTTHSMALIHNRSMFSDKQMIKLQESPEDMPAGQTPHTTILYGHNDLVDKVQPGDRVNVTGIYRAVPIRVNPRVRNVKSVYKTHIDVIHYRKTDSKRLHGIDEDTEQKLFTEERVAMLKELAAKPDIYERLAAALAPSIYEHEDIKKGILLQLFGGTRKDFSHTGRGKFRAEVNILLCGDPGTSKSQLLQYVFNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPVESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQMKEEHLDMAVLKDYIAYARTYVNPRLSEEASQALIEAYVSMRKIGSGRGMVSAYPRQLESLIRLSEAHAKVRFSNKVETIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATARKRKEELAQVLKKLIQSKGKTPALKYQQLFEDLRGQSDAAITKDMFDEALHALADDDYLTVTGKTVRLL CA05A827EE16803D mcm7a_xenla DNA replication licensing factor mcm7-A Minichromosome maintenance protein 7-A CDC47 homolog A CDC47p p90 mcm7-a cdc47 protein protein xenla Xenopus laevis (African clawed frog) African clawed frog MPRDYQTEKEKCKTFLQEFYKDDEIGKKHFKYGVQLANIAHREQVALYIDLDDLAEEDPELVDAICENTRRYTNLFADAVQELLPQYKEREVVHKDALDVYIEHRLMMEQRGRDPNEMRDSQNQYPPELMRRFELYFKAPSSSKARVVRDVKADSIGKLVNIRGIVTRVTEVKPMMVVATYTCDQCGAETYQPIQSPTFMPLIMCPSRECQTNRSGGRLYLQTRGSKFIKFQELKIQEHSDQVPVGNIPRCMSVYVRGENTRLAQPGDHVGITGVFLPMLRTGFRQVVQGLLSETYLECHRLVKMNKSEDDELGTEELSEEELRQITEEDFYEKLAASIAPEIYGHEDVKKALLLLLVGGVDNSPRGMKIRGNINICLMGDPGVAKSQLLSYIDRLAPRSQYTTGRGSSGVGLTAAVMKDPVTGEMTLEGGALVLADQGVCCIDEFDKMMDTDRTAIHEVMEQQTISIAKAGIMTTLNARCSILAAANPAYGRYNPKKTVEQNIQLPAALLSRFDVLWLIQDKPDRDNDLRLAQHITYVHQHSKQPPSQFQPLDMKLMRRYITMCKRKQPAIPEALADYLTAAYVEMRKEARTNKDMTFTSARTLLSVLRLSTALARLRLEDVVEKEDVNEAMRLMEMSKDSLLGDKGHTSRTQRPADVIFSTIREMVPEKGARSVKYSEAEQRCVSKGFTPAQFEAALEEYEELNVWLVNQARTKITFV AD623D73B501647E mcm2-mcm3-1 1 4 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 7 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 5 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 6 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 8 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection association association Complex has ATPase but not DNA helicase activity A glycerol density gradient step performed as part of the purification of this complexes, subsequent to pulldown indicated an approximate molecular weight of 600kDa suggesting that the complex is a hexamer. mcm4-mcm6-2 1 7 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 8 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection association association Complex has DNA helicase activity mcm2-mcm3-2 2 4 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 6 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role bait bait spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 8 unspecified role unspecified role prey prey c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection association association mcm4-mcm6-3 2 7 unspecified role unspecified role prey prey spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 3 unspecified role unspecified role bait bait spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 8 unspecified role unspecified role prey prey c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection association association Complex has DNA helicase activity