UniProt UniProt http://www.uniprot.org Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres. Mis16 and Mis18 are required for CENP-A loading and histone deacetylation at centromeres. Cell (0092-8674) 2004 Hayashi T., Fujita Y., Iwasaki O., Adachi Y., Takahashi K., Yanagida M. Chromatin - Epigenetic interactions resulting in chromatin modulation yanagida@kozo.lif.kyoto-u.ac.jp 13-NOV-2006: Contacted by JYOTI. schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hayashi T., Fujita Y., Iwasaki O., Adachi Y., Takahashi K., Yanagida M. Chromatin - Epigenetic interactions resulting in chromatin modulation yanagida@kozo.lif.kyoto-u.ac.jp Accepted 2006-NOV-1 by JYOTI. 13-NOV-2006: Contacted by JYOTI. Cell (0092-8674) 2004 hat2_schpo Histone acetyltransferase type B subunit 2 Kinetochore protein mis16 mis16 SPCC1672.10 hat2 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSEEVVQDAPLENNELNAEIDLQKTIQEEYKLWKQNVPFLYDLVITHALEWPSLTIQWLPDKKTIPGTDYSIQRLILGTHTSGNDQNYLQIASVQLPNFDEDTTEFTPSTIRRAQATGSYTIEISQKIPHDGDVNRARYMPQKPEIIATMGEGGNAYIFDTTCHDALTTGEALPQAVLKGHTAEGFGLCWNPNLPGNLATGAEDQVICLWDVQTQSFTSSETKVISPIAKYHRHTDIVNDVQFHPQHEALLASVSDDCTLQIHDTRLNPEEEAPKVIQAHSKAINAVAINPFNDYLLATASADKTVALWDLRNPYQRLHTLEGHEDEVYGLEWSPHDEPILASSSTDRRVCIWDLEKIGEEQTPEDAEDGSPELLFMHGGHTNRISEFSWCPNERWVVGSLADDNILQIWSPSRVIWGRDHVQVSPRDLE 606A7D009E60EA20 cenpu_schpo Inner kinetochore subunit mis17 Sim4 complex subunit mis17 SPBC21.01 SPBC776.19 mis17 CENP-U homolog Constitutive centromere-associated network protein mis17 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MENNSHNVDERRAARMRGAERYKIQNVEFSLDQNLLANLESSTSSSTRSSPASNQQNLEIVSQSEAYHKISDLDDSTSIFSISDQENILERRISEEVVRENSVSSIENVSSSAVARETQSSANILVKDNHFFSHKQKKIQRESIPFHKRDNIETSDAYSSSILENSPPNKVQRLSSLDSSQDSFQEEHPGNVTGTTFSSQAPEERIASPISTSSPESLTNQSSSLQSSLQTSSMAPRNLLDQSTEKDIVVGASDELVRIELTKLSKEAGGSKTLNELDAVQQFFQEFIKENEPLEPYVVKVKNAFVEQVSIRLLELIDLLDANRILSTACKKAANEKLAVQRDLSKLREDRLSVQRKKIQLRNEYIKLSHQQNFLDDIDDFFSQCETVKNELENVTTTPNSTEAFSEINEYASALSKNYGLESQLVELQSLLTQFYQKFLS A65FD10DC3DA4C98 mis14_schpo Kinetochore protein mis14 NMS complex subunit mis14 SPAC688.02c mis14 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSHGDASVLPKIALESINDYSYVKSVFRQAIQEKLAIHLPEQASREAGMGREDQLRIRVKEMLNELIESTFSIIQENVTINGFDANSALQDPKNSDQIEPFDLALRTRVQQLFNEVEDAHVLVARYRKSVPAQYEKAYVDAMEQQTAFLRNVKDDYVSLQDKEVENPDEQTSTTVFRKEDIDRYEQTIAKVAYLKKNLPRVVARLEKTP 400DC20681812390 cenpn_schpo Inner kinetochore subunit mis15 Sim4 complex subunit mis15 SPBP22H7.09c pi022 mis15 CENP-N homolog Constitutive centromere-associated network protein mis15 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MRTELSSNIRIKHDKKIQKLLNRFPRDFLVKLCVEWIQKQTYPPNAKDINLEDMLDDEEWNPEAFYKNVPKSMLKRSIIHRMLVYDWPNGFYLGQIAQLEILALAHGFVSMRWTASKVHHSAEKTVLPNPLVFLELLKSELESIFVYHTYISRHETLPITFIRLVLWDSKRPTALHTYPSSKQIFYLGLMDDSDVLLHNIFLRNDVCHSLFLQCLSRLLYRLKAGSALRPIDLVSKNLTTFCTNVGVNKEANALGAWQIYAKNLVDRSPLDTRPILSDDNSSLIADSTQNCEKHREMAIQRRFGDTHSQVLDKLLITLDHDYIEKSTEKDKVLEENVESYNPTEQRPLVVMQLRGQHILEGLKDICRQDALDPFTMPSYLTGETGLSILYVRDGLVLQRQESLGQQEIN 60938B407FB66897 cenpi_schpo Inner kinetochore subunit mis6 Sim4 complex subunit mis6 mis6 SPAC1687.20c CENP-I homolog Constitutive centromere-associated network protein mis6 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MESFENKGFLDIEEGIQWIKKNSENLSSSKKTILARLQQFHKLCSEVGLNQHSISSLLDVILSKKTHFDKNHVQLLIKCLYPNELISQTIAIRIISSLDPHGLRCSYAIQAKLLNWLIHVYEFLDGNNLLCRYYGVLFHFLDFLTLRPYISNLLVLLTKHYHVKSFRIHQLLALYQKPGNTADPYLLALILTYKQHFPDVIVGSYTYRKHGSVRLDSEWIAATKAILNRQSEDVPLETWSSEKRKRQSSLIPDLITMKNTSSSYSLEELTSVQQMGLVYEKIVFPSRIAAVLKSKLFLIFLFLKNKNVYYSRLDEWLHITLNYGLALRSGSNNQEEEVLHLLYKYLLFSPKFPKSLLQYVITFFSKPNITEENYNLLTLLVTHIPITTDSSYFNSLLKEFEQFILQKNAEFCSKHLNILWLWLFRMLNLRIASMGNNHTLLEKCLLITNHATFLVSHFSWDVSLAYQLSRLFQLYYKILTKIRKQIEPNIPPKELIYVLFFQPSAFYINSMVGLLLLTKNYQERLMDSRIDAISKFTHSYLKSLSEIILLKEKRAILSFLQLWEPFKSDYSQFLPIATRIANDHPYAQRVFSLTCAPQFFSYINGYQIYLQQTNPATGSIPLKPIQEETFGAFQSNLHLSDSWEDFQKNFIIYLKKKGYLAISDFLLSTLNR 4E2B7FEDD567174F mis18_schpo Kinetochore protein mis18 SPCC970.12 mis18 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSQTETSHSGYIDFKKESQPSVFQCKKCFQIVGDSNAWVISHREYLSFTLSDAVENSVRVEDTFKRSDDGLCVYSELSCTRCNEVIGKVYNSTPIYLDDIRDMYTFSMDKLQAYQLGNKTVNPEGLTRYQVDLEMREDIIKLKSFCLSLYEKFELHDETLRSVKETISSLKKPKIEGKEGKKEKARTYSKRTRK 5D9C13F1E3D68193 mis12_schpo Centromere protein mis12 NMS complex subunit mis12 mis12 SPBC409.04c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MLVELLEFTPLSFIDDVINITNQLLYKGVNGVDKAFSQTRFAKKAPQEIEEGLHKFEVLFESVVDRYYDGFEVYTLRNIFSYPPELKGYMRTFGKDVDYSITTEQDAAMDQAIQEAAEKLVVKMQLRRDLRMRLSRKREKKTEIEKHLERISFLNKVPENWQVTLPETTDFLLDQLGNLQHAVKRVVEASPTVHSREVDERITYLEKGYERLSNPIDQQKDFWSHHLSKLESTANTETANNIHKLLLSSEKDVGHTDEP B4D0B64BB9EB9007 mis15-mis6-1 1 6 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 4B mis17-mis6-1 1 6 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 4B mis14-mis12-1 1 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 4B mis17-mis15-1 1 5 unspecified role unspecified role prey prey c-terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 3 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 4C mis16-mis18-1 1 7 unspecified role unspecified role prey prey c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 2 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 6C mis18-mis16-1 1 7 unspecified role unspecified role bait bait c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 2 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 6C