IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein. Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein. J. Biol. Chem. (0021-9258) 2004 Bondos SE., Catanese DJ., Tan XX., Bicknell A., Li L., Matthews KS. Chromatin - Epigenetic interactions resulting in chromatin modulation Embryonic cDNA library used in experiment. in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Bondos SE., Catanese DJ., Tan XX., Bicknell A., Li L., Matthews KS. Chromatin - Epigenetic interactions resulting in chromatin modulation Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Embryonic cDNA library used in experiment. J. Biol. Chem. (0021-9258) 2004 Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein. Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein. J. Biol. Chem. (0021-9258) 2004 Bondos SE., Catanese DJ., Tan XX., Bicknell A., Li L., Matthews KS. Chromatin - Epigenetic interactions resulting in chromatin modulation Embryonic cDNA library used in experiment. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid full dna sequence full identification by DNA sequencing full dna sequence Bondos SE., Catanese DJ., Tan XX., Bicknell A., Li L., Matthews KS. Chromatin - Epigenetic interactions resulting in chromatin modulation Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Embryonic cDNA library used in experiment. J. Biol. Chem. (0021-9258) 2004 Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein. Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein. J. Biol. Chem. (0021-9258) 2004 Bondos SE., Catanese DJ., Tan XX., Bicknell A., Li L., Matthews KS. Chromatin - Epigenetic interactions resulting in chromatin modulation Embryonic cDNA library used in experiment. in vitro In vitro phage display phage display predetermined predetermined participant predetermined Bondos SE., Catanese DJ., Tan XX., Bicknell A., Li L., Matthews KS. Chromatin - Epigenetic interactions resulting in chromatin modulation Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Embryonic cDNA library used in experiment. J. Biol. Chem. (0021-9258) 2004 Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein. Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein. J. Biol. Chem. (0021-9258) 2004 Bondos SE., Catanese DJ., Tan XX., Bicknell A., Li L., Matthews KS. Chromatin - Epigenetic interactions resulting in chromatin modulation Embryonic cDNA library used in experiment. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Bondos SE., Catanese DJ., Tan XX., Bicknell A., Li L., Matthews KS. Chromatin - Epigenetic interactions resulting in chromatin modulation Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Embryonic cDNA library used in experiment. J. Biol. Chem. (0021-9258) 2004 Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein. Hox transcription factor ultrabithorax Ib physically and genetically interacts with disconnected interacting protein 1, a double-stranded RNA-binding protein. J. Biol. Chem. (0021-9258) 2004 Bondos SE., Catanese DJ., Tan XX., Bicknell A., Li L., Matthews KS. Chromatin - Epigenetic interactions resulting in chromatin modulation Embryonic cDNA library used in experiment. in vitro In vitro coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Bondos SE., Catanese DJ., Tan XX., Bicknell A., Li L., Matthews KS. Chromatin - Epigenetic interactions resulting in chromatin modulation Accepted 2010-JAN-10 by SKERRIEN (applied to all records created before 2005-Sep-01). Embryonic cDNA library used in experiment. J. Biol. Chem. (0021-9258) 2004 q9tw27_drome DIP1-RD DIP1 dip1 Dmel\CG17686 TO34 TO67 UbxBP1 clot 10495 clot 2020 dip1a klt Dmel_CG17686 CG17686 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKRNRRAFAGNNKPFVFGGIYNPNATVKPLIQMPLQAANVQEQQSSPVALYPTSASPVAVQSPQDAFLAVQSPGAPAPVQLANSSDQQNNPDADAIASKLPMPVIIKEEPISVNDEPSVDNIEDNTSASTSASGIGGKIPFKKIFQKRKKSSERTRDKKLRQNRQLRKSMLPKNALMALNEVKGVTISDFTIDSNTDGGFTAVVTVNSNQYEGKGTSKMTAKNAACEKAWRDFIIAKMTPKPPRIHQVEMGSEPMDINEDEADAPDDDLPMLNLASFAIYKLFAEWEREGYVVPEMHPSANAAQQAGGDAGTPVPPVPKEPKKPPVRTELPSGWETMHPATILCIMRPGLNYVDYGSSGDKTNGMQHLGIMVDNQEFHANGRSKKIARRNVAVKVCNSLFGTNFTYSDTT 3A19D17A95385101 p83949-1 Homeotic protein ultrabithorax A Ubx CG10388 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNSYFEQASGFYGHPHQATGMAMGSGGHHDQTASAAAAAYRGFPLSLGMSPYANHHLQRTTQDSPYDASITAACNKIYGDGAGAYKQDCLNIKADAVNGYKDIWNTGGSNGGGGGGGGGGGGGAGGTGGAGNANGGNAANANGQNNPAGGMPVRPSACTPDSRVGGYLDTSGGSPVSHRGGSAGGNVSVSGGNGNAGGVQSGVGVAGAGTAWNANCTISGAAAQTAAASSLHQASNHTFYPWMAIAGECPEDPTKSKIRSDLTQYGGISTDMGKRYSESLAGSLLPDWLGTNGLRRRGRQTYTRYQTLELEKEFHTNHYLTRRRRIEMAHALCLTERQIKIWFQNRRMKLKKEIQAIKELNEQEKQAQAQKAAAAAAAAAAVQGGHLDQ Has been shown to exist only in D.simulans so far D452E8FFE55D8F53 ubx-dip1-1 2 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association UBX with deletion of the N-terminal 216 residues used in the Y2H. DIP1c long isoform was obtained from the cDNA library. Revisit when protein editor in place. Revisit when Swiss-prot isoform available. ubx-dip1-2 4 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Revisit when protein editor in place. Revisit when Swiss-prot isoform available. DIP1c long isoform was obtained from the cDNA library. ubx-dip1-3 4 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association Revisit when protein editor in place. Revisit when Swiss-prot isoform available. DIP1c long isoform was obtained from the cDNA library. ubx-dip1-4 4 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Revisit when protein editor in place. Revisit when Swiss-prot isoform available. DIP1c long isoform was obtained from the cDNA library. The UBX had A223P/A226P/Q233P changes. ubx-dip1-5 3 7 unspecified role unspecified role bait bait ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role prey prey ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Revisit when protein editor in place. Revisit when Swiss-prot isoform available. DIP1c long isoform was obtained from the cDNA library. ubx-dip1-6 5 7 unspecified role unspecified role bait bait ecobb Escherichia coli (strain B / BL21) 6 unspecified role unspecified role prey prey ecobb Escherichia coli (strain B / BL21) physical association physical association Revisit when protein editor in place. Revisit when Swiss-prot isoform available. DIP1c long isoform was obtained from the cDNA library. Antibodies against the N and C terminii of the protein were used and gave similar results. DIP1-c has a 6 HIS N-terminal tag. ubx-dip1-7 1 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey physical association physical association Revisit when protein editor in place. Revisit when Swiss-prot isoform available. DIP1c long isoform was obtained from the cDNA library. UBX was GST tagged. Similar results were observed when His6 tagged DIP was used and anti His antibodies were used for detection.