IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Oncogene (0950-9232) 2003 imex curation Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Obunguvi@lilly.com 01-DEC-2006: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Obunguvi@lilly.com Accepted 2006-NOV-15 by DAVET. 01-DEC-2006: Contacted by JYOTI. Oncogene (0950-9232) 2003 Only protein-protein interactions imex curation Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Oncogene (0950-9232) 2003 imex curation Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Obunguvi@lilly.com 01-DEC-2006: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 tap tandem affinity purification TAP TAP tap western blot western blot Immuno blot Immuno blot Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Obunguvi@lilly.com Accepted 2006-NOV-15 by DAVET. 01-DEC-2006: Contacted by JYOTI. Oncogene (0950-9232) 2003 Only protein-protein interactions imex curation Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Oncogene (0950-9232) 2003 imex curation Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Obunguvi@lilly.com 01-DEC-2006: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Obunguvi@lilly.com Accepted 2006-NOV-15 by DAVET. 01-DEC-2006: Contacted by JYOTI. Oncogene (0950-9232) 2003 Only protein-protein interactions imex curation Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Oncogene (0950-9232) 2003 imex curation Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Obunguvi@lilly.com 01-DEC-2006: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Washing steps were done in high salt (500nM NaCl) Obunguvi@lilly.com Accepted 2006-NOV-15 by DAVET. 01-DEC-2006: Contacted by JYOTI. Oncogene (0950-9232) 2003 Only protein-protein interactions imex curation Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Oncogene (0950-9232) 2003 imex curation Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Obunguvi@lilly.com 01-DEC-2006: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 cosedimentation cosedimentation western blot western blot Immuno blot Immuno blot Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Interaction was detected using myosin purification by actin selection in which F-actin and phalloidin (5 micromolar each) were added to the cell extract, then sedimented and solubilized in the presence or absence of ATP. ATP causes release of myosin together with any proteins associated with it in the same fraction. Obunguvi@lilly.com Accepted 2006-NOV-15 by DAVET. 01-DEC-2006: Contacted by JYOTI. Oncogene (0950-9232) 2003 Only protein-protein interactions imex curation Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Menin, a tumor suppressor, associates with nonmuscle myosin II-A heavy chain. Oncogene (0950-9232) 2003 imex curation Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Obunguvi@lilly.com 01-DEC-2006: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Obungu VH., Lee Burns A., Agarwal SK., Chandrasekharapa SC., Adelstein RS., Marx SJ. Chromatin - Epigenetic interactions resulting in chromatin modulation Obunguvi@lilly.com Accepted 2006-NOV-15 by DAVET. 01-DEC-2006: Contacted by JYOTI. Oncogene (0950-9232) 2003 Only protein-protein interactions imex curation o00255-2 Menin Short MEN1 SCG2 protein protein human Homo sapiens Human MGLKAAQKTLFPLRSIDDVVRLFAAELGREEPDLVLLSLVLGFVEHFLAVNRVIPTNVPELTFQPSPAPDPPGGLTYFPVADLSIIAALYARFTAQIRGAVDLSLYPREGGVSSRELVKKVSDVIWNSLSRSYFKDRAHIQSLFSFITGTKLDSSGVAFAVVGACQALGLRDVHLALSEDHAWVVFGPNGEQTAEVTWHGKGNEDRRGQTVNAGVAERSWLYLKGSYMRCDRKMEVAFMVCAINPSIDLHTDSLELLQLQQKLLWLLYDLGHLERYPMALGNLADLEELEPTPGRPDPLTLYHKGIASAKTYYRDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQDYNYCREDEEIYKEFFEVANDVIPNLLKEAASLLEAGEERPGEQSQGTQSQGSALQDPECFAHLLRFYDGICKWEEGSPTPVLHVGWATFLVQSLGRFEGQVRQKVRIVSREAEAAEAEEPWGEEAREGRRRGPRRESKPEEPPPPKKPALDKGLGTGQGAVSGPPRKPPGTVAGTARGPEGGSTAQVPAPTASPPPEGPVLTFQSEKMKGMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL DIP protein O00255-2 original sequence version: 116 9BF844C0302B43EF men1_human Menin MEN1 SCG2 protein protein human Homo sapiens Human MGLKAAQKTLFPLRSIDDVVRLFAAELGREEPDLVLLSLVLGFVEHFLAVNRVIPTNVPELTFQPSPAPDPPGGLTYFPVADLSIIAALYARFTAQIRGAVDLSLYPREGGVSSRELVKKVSDVIWNSLSRSYFKDRAHIQSLFSFITGWSPVGTKLDSSGVAFAVVGACQALGLRDVHLALSEDHAWVVFGPNGEQTAEVTWHGKGNEDRRGQTVNAGVAERSWLYLKGSYMRCDRKMEVAFMVCAINPSIDLHTDSLELLQLQQKLLWLLYDLGHLERYPMALGNLADLEELEPTPGRPDPLTLYHKGIASAKTYYRDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQDYNYCREDEEIYKEFFEVANDVIPNLLKEAASLLEAGEERPGEQSQGTQSQGSALQDPECFAHLLRFYDGICKWEEGSPTPVLHVGWATFLVQSLGRFEGQVRQKVRIVSREAEAAEAEEPWGEEAREGRRRGPRRESKPEEPPPPKKPALDKGLGTGQGAVSGPPRKPPGTVAGTARGPEGGSTAQVPAPTASPPPEGPVLTFQSEKMKGMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL DIP protein O00255 original sequence version: 100 DIP protein O00255 original sequence version: 131 DDDF850EA5AB77B4 myh9_human Myosin-9 MYH9 Myosin heavy chain 9 Myosin heavy chain, non-muscle IIa Non-muscle myosin heavy chain IIa Cellular myosin heavy chain, type A Non-muscle myosin heavy chain A protein protein human Homo sapiens Human MAQQAADKYLYVDKNFINNPLAQADWAAKKLVWVPSDKSGFEPASLKEEVGEEAIVELVENGKKVKVNKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLKERYYSGLIYTYSGLFCVVINPYKNLPIYSEEIVEMYKGKKRHEMPPHIYAITDTAYRSMMQDREDQSILCTGESGAGKTENTKKVIQYLAYVASSHKSKKDQGELERQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVNGYIVGANIETYLLEKSRAIRQAKEERTFHIFYYLLSGAGEHLKTDLLLEPYNKYRFLSNGHVTIPGQQDKDMFQETMEAMRIMGIPEEEQMGLLRVISGVLQLGNIVFKKERNTDQASMPDNTAAQKVSHLLGINVTDFTRGILTPRIKVGRDYVQKAQTKEQADFAIEALAKATYERMFRWLVLRINKALDKTKRQGASFIGILDIAGFEIFDLNSFEQLCINYTNEKLQQLFNHTMFILEQEEYQREGIEWNFIDFGLDLQPCIDLIEKPAGPPGILALLDEECWFPKATDKSFVEKVMQEQGTHPKFQKPKQLKDKADFCIIHYAGKVDYKADEWLMKNMDPLNDNIATLLHQSSDKFVSELWKDVDRIIGLDQVAGMSETALPGAFKTRKGMFRTVGQLYKEQLAKLMATLRNTNPNFVRCIIPNHEKKAGKLDPHLVLDQLRCNGVLEGIRICRQGFPNRVVFQEFRQRYEILTPNSIPKGFMDGKQACVLMIKALELDSNLYRIGQSKVFFRAGVLAHLEEERDLKITDVIIGFQACCRGYLARKAFAKRQQQLTAMKVLQRNCAAYLKLRNWQWWRLFTKVKPLLQVSRQEEEMMAKEEELVKVREKQLAAENRLTEMETLQSQLMAEKLQLQEQLQAETELCAEAEELRARLTAKKQELEEICHDLEARVEEEEERCQHLQAEKKKMQQNIQELEEQLEEEESARQKLQLEKVTTEAKLKKLEEEQIILEDQNCKLAKEKKLLEDRIAEFTTNLTEEEEKSKSLAKLKNKHEAMITDLEERLRREEKQRQELEKTRRKLEGDSTDLSDQIAELQAQIAELKMQLAKKEEELQAALARVEEEAAQKNMALKKIRELESQISELQEDLESERASRNKAEKQKRDLGEELEALKTELEDTLDSTAAQQELRSKREQEVNILKKTLEEEAKTHEAQIQEMRQKHSQAVEELAEQLEQTKRVKANLEKAKQTLENERGELANEVKVLLQGKGDSEHKRKKVEAQLQELQVKFNEGERVRTELADKVTKLQVELDNVTGLLSQSDSKSSKLTKDFSALESQLQDTQELLQEENRQKLSLSTKLKQVEDEKNSFREQLEEEEEAKHNLEKQIATLHAQVADMKKKMEDSVGCLETAEEVKRKLQKDLEGLSQRHEEKVAAYDKLEKTKTRLQQELDDLLVDLDHQRQSACNLEKKQKKFDQLLAEEKTISAKYAEERDRAEAEAREKETKALSLARALEEAMEQKAELERLNKQFRTEMEDLMSSKDDVGKSVHELEKSKRALEQQVEEMKTQLEELEDELQATEDAKLRLEVNLQAMKAQFERDLQGRDEQSEEKKKQLVRQVREMEAELEDERKQRSMAVAARKKLEMDLKDLEAHIDSANKNRDEAIKQLRKLQAQMKDCMRELDDTRASREEILAQAKENEKKLKSMEAEMIQLQEELAAAERAKRQAQQERDELADEIANSSGKGALALEEKRRLEARIAQLEEELEEEQGNTELINDRLKKANLQIDQINTDLNLERSHAQKNENARQQLERQNKELKVKLQEMEGTVKSKYKASITALEAKIAQLEEQLDNETKERQAACKQVRRTEKKLKDVLLQVDDERRNAEQYKDQADKASTRLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE DIP protein P35579 original sequence version: 126 DIP protein P35579-1 original sequence version: 142 DIP protein P35579-1 original sequence version: 133 DIP protein P35579 original sequence version: 186 DIP protein P35579-1 original sequence version: 186 DIP protein P35579-1 original sequence version: 146 DIP protein P35579 original sequence version: 146 588F84BB8C106E6F menin-myh9-1 6 7 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role prey prey physical association physical association 2a Two bands were unambiguously identified from the eluates obtained from TAP purification as follows: the band migrating at about 72 kDa was identified as menin, while that migrating at about 200 kDa was identified as NMHC II-A. menin-myh9-2 2 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 2b While endogenous NMHC II-A was present in both crude extracts, it copurified with menin�tap but not with tap alone. menin-myh9-3 3 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 3 The resulting immunoprecipitate was enriched not only in menin but also in NMHC II-A menin-myh9-4 4 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction 6a NMHCII-A bound to GST-menin but not to GST alone. myh9-menin-1 4 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain O00255-2:p.Pro12Leu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P L Wed May 02 14:44:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro12leu' Tue Jun 30 17:46:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro12Leu' O00255-2:p.Leu22Arg mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L R Wed May 02 20:27:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu22arg' Tue Jun 30 15:48:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu22Arg' O00255-2:p.His139Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain H D Wed May 02 18:56:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his139asp' Tue Jun 30 16:47:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His139Asp' O00255-2:p.Phe159Cys mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F C Wed May 02 15:46:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe159cys' Tue Jun 30 17:53:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe159Cys' O00255-2:p.Ala164Asp mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A D Wed May 02 17:47:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala164asp' Tue Jun 30 18:54:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala164Asp' O00255-2:p.Trp183Ser mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W S Wed May 02 17:49:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp183ser' Tue Jun 30 18:00:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp183Ser' 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction 6a and 7 Menin interacts with NMHC II-A in vitro by GST pulldown. menin-myh9-5 1 9 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 8 Menin and NMHC II-A colocalize in the cleavage furrow by indirect immunofluoresence microscopy. menin-myh9-6 5 9 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component physical association physical association 5 Interaction occurs in the presence of F-actin crosslinked by 5 um phalloidin and was ATP dependent. this further confirms that menin associates tightly with NMHC II-A and remains bound to it upon myosin release from actin after addition of ATP.