IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation. BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation. Mol. Cell. Biol. (0270-7306) 2003 imex curation Hsiao PW., Fryer CJ., Trotter KW., Wang W., Archer TK. Chromatin - Epigenetic interactions resulting in chromatin modulation archer1@niehs.nih.gov 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions human-sw13 human sw13 cells from bone sw13 SW1353 Species: human, Caucasian female 72 years old; Tissue: bone; Tumor: chondrosarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hsiao PW., Fryer CJ., Trotter KW., Wang W., Archer TK. Chromatin - Epigenetic interactions resulting in chromatin modulation archer1@niehs.nih.gov Accepted 2007-OCT-4 by JYOTI. 12-Oct-2007: Contacted by JYOTI. Mol. Cell. Biol. (0270-7306) 2003 Only protein-protein interactions imex curation BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation. BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation. Mol. Cell. Biol. (0270-7306) 2003 imex curation Hsiao PW., Fryer CJ., Trotter KW., Wang W., Archer TK. Chromatin - Epigenetic interactions resulting in chromatin modulation archer1@niehs.nih.gov 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hsiao PW., Fryer CJ., Trotter KW., Wang W., Archer TK. Chromatin - Epigenetic interactions resulting in chromatin modulation archer1@niehs.nih.gov Accepted 2007-OCT-4 by JYOTI. Mol. Cell. Biol. (0270-7306) 2003 Only protein-protein interactions imex curation BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation. BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation. Mol. Cell. Biol. (0270-7306) 2003 imex curation Hsiao PW., Fryer CJ., Trotter KW., Wang W., Archer TK. Chromatin - Epigenetic interactions resulting in chromatin modulation archer1@niehs.nih.gov 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hsiao PW., Fryer CJ., Trotter KW., Wang W., Archer TK. Chromatin - Epigenetic interactions resulting in chromatin modulation archer1@niehs.nih.gov Accepted 2007-OCT-4 by JYOTI. 12-Oct-2007: Contacted by JYOTI. Mol. Cell. Biol. (0270-7306) 2003 Only protein-protein interactions imex curation BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation. BAF60a mediates critical interactions between nuclear receptors and the BRG1 chromatin-remodeling complex for transactivation. Mol. Cell. Biol. (0270-7306) 2003 imex curation Hsiao PW., Fryer CJ., Trotter KW., Wang W., Archer TK. Chromatin - Epigenetic interactions resulting in chromatin modulation archer1@niehs.nih.gov 12-Oct-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Hsiao PW., Fryer CJ., Trotter KW., Wang W., Archer TK. Chromatin - Epigenetic interactions resulting in chromatin modulation archer1@niehs.nih.gov Accepted 2007-OCT-4 by JYOTI. 12-Oct-2007: Contacted by JYOTI. Mol. Cell. Biol. (0270-7306) 2003 Only protein-protein interactions imex curation nr1h4_human Bile acid receptor NR1H4 BAR FXR HRR1 RIP14 Farnesoid X-activated receptor Farnesol receptor HRR-1 Nuclear receptor subfamily 1 group H member 4 Retinoid X receptor-interacting protein 14 protein protein human Homo sapiens Human MVMQFQGLENPIQISPHCSCTPSGFFMEMMSMKPAKGVLTEQVAGPLGQNLEVEPYSQYSNVQFPQVQPQISSSSYYSNLGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVAEMPVTKKPRMGASAGRIKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQECRLRKCKEMGMLAECMYTGLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCREKTELTPDQQTLLHFIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATNHVQVLVEFTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPSGHSDLLEERIRNSGISDEYITPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCKIHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ C23283576A8CF76B gcr_rat Glucocorticoid receptor Nuclear receptor subfamily 3 group C member 1 Nr3c1 Grl protein protein rat Rattus norvegicus (Rat) Rat MDSKESLAPPGRDEVPGSLLGQGRGSVMDFYKSLRGGATVKVSASSPSVAAASQADSKQQRILLDFSKGSTSNVQQRQQQQQQQQQQQQQQQQQQQPDLSKAVSLSMGLYMGETETKVMGNDLGYPQQGQLGLSSGETDFRLLEESIANLNRSTSVPENPKSSTSATGCATPTEKEFPKTHSDASSEQQNRKSQTGTNGGSVKLYPTDQSTFDLLKDLEFSAGSPSKDTNESPWRSDLLIDENLLSPLAGEDDPFLLEGNTNEDCKPLILPDTKPKIKDTGDTILSSPSSVALPQVKTEKDDFIELCTPGVIKQEKLGPVYCQASFSGTNIIGNKMSAISVHGVSTSGGQMYHYDMNTASLSQQQDQKPVFNVIPPIPVGSENWNRCQGSGEDSLTSLGALNFPGRSVFSNGYSSPGMRPDVSSPPSSSSAATGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGRNDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGIQQATAGVSQDTSENPNKTIVPAALPQLTPTLVSLLEVIEPEVLYAGYDSSVPDSAWRIMTTLNMLGGRQVIAAVKWAKAILGLRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSGNLLCFAPDLIINEQRMSLPCMYDQCKHMLFVSSELQRLQVSYEEYLCMKTLLLLSSVPKEGLKSQELFDEIRMTYIKELGKAIVKREGNSSQNWQRFYQLTKLLDSMHEVVENLLTYCFQTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFHQK DIP protein P06536 original sequence version: 183 DIP protein P06536 original sequence version: 143 DIP protein P06536 original sequence version: 139 9C9DE0B1D6724845 esr1_human Estrogen receptor ESR1 ESR NR3A1 Estradiol receptor ER-alpha Nuclear receptor subfamily 3 group A member 1 protein protein human Homo sapiens Human MTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCNDYASGYHYGVWSCEGCKAFFKRSIQGHNDYMCPATNQCTIDKNRRKSCQACRLRKCYEVGMMKGGIRKDRRGGRMLKHKRQRDDGEGRGEVGSAGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSLQKYYITGEAEGFPATV DIP protein P03372 original sequence version: 214 DIP protein P03372 original sequence version: 139 DIP protein P03372 original sequence version: 167 5455C57AB0CCCAA7 ncoa3_human Nuclear receptor coactivator 3 NCOA3 AIB1 RAC3 Thyroid hormone receptor activator molecule 1 Receptor-associated coactivator 3 BHLHE42 Steroid receptor coactivator protein 3 CBP-interacting protein Amplified in breast cancer 1 protein ACTR Class E basic helix-loop-helix protein 42 TRAM1 protein protein human Homo sapiens Human MSGLGENLDPLASDSRKRKLPCDTPGQGLTCSGEKRRREQESKYIEELAELISANLSDIDNFNVKPDKCAILKETVRQIRQIKEQGKTISNDDDVQKADVSSTGQGVIDKDSLGPLLLQALDGFLFVVNRDGNIVFVSENVTQYLQYKQEDLVNTSVYNILHEEDRKDFLKNLPKSTVNGVSWTNETQRQKSHTFNCRMLMKTPHDILEDINASPEMRQRYETMQCFALSQPRAMMEEGEDLQSCMICVARRITTGERTFPSNPESFITRHDLSGKVVNIDTNSLRSSMRPGFEDIIRRCIQRFFSLNDGQSWSQKRHYQEAYLNGHAETPVYRFSLADGTIVTAQTKSKLFRNPVTNDRHGFVSTHFLQREQNGYRPNPNPVGQGIRPPMAGCNSSVGGMSMSPNQGLQMPSSRAYGLADPSTTGQMSGARYGGSSNIASLTPGPGMQSPSSYQNNNYGLNMSSPPHGSPGLAPNQQNIMISPRNRGSPKIASHQFSPVAGVHSPMASSGNTGNHSFSSSSLSALQAISEGVGTSLLSTLSSPGPKLDNSPNMNITQPSKVSNQDSKSPLGFYCDQNPVESSMCQSNSRDHLSDKESKESSVEGAENQRGPLESKGHKKLLQLLTCSSDDRGHSSLTNSPLDSSCKESSVSVTSPSGVSSSTSGGVSSTSNMHGSLLQEKHRILHKLLQNGNSPAEVAKITAEATGKDTSSITSCGDGNVVKQEQLSPKKKENNALLRYLLDRDDPSDALSKELQPQVEGVDNKMSQCTSSTIPSSSQEKDPKIKTETSEEGSGDLDNLDAILGDLTSSDFYNNSISSNGSHLGTKQQVFQGTNSLGLKSSQSVQSIRPPYNRAVSLDSPVSVGSSPPVKNISAFPMLPKQPMLGGNPRMMDSQENYGSSMGGPNRNVTVTQTPSSGDWGLPNSKAGRMEPMNSNSMGRPGGDYNTSLPRPALGGSIPTLPLRSNSIPGARPVLQQQQQMLQMRPGEIPMGMGANPYGQAAASNQLGSWPDGMLSMEQVSHGTQNRPLLRNSLDDLVGPPSNLEGQSDERALLDQLHTLLSNTDATGLEEIDRALGIPELVNQGQALEPKQDAFQGQEAAVMMDQKAGLYGQTYPAQGPPMQGGFHLQGQSPSFNSMMNQMNQQGNFPLQGMHPRANIMRPRTNTPKQLRMQLQQRLQGQQFLNQSRQALELKMENPTAGGAAVMRPMMQPQVSSQQGFLNAQMVAQRSRELLSHHFRQQRVAMMMQQQQQQQQQQQQQQQQQQQQQQQQQQQQQTQAFSPPPNVTASPSMDGLLAGPTMPQAPPQQFPYQPNYGMGQQPDPAFGRVSSPPNAMMSSRMGPSQNPMMQHPQAASIYQSSEMKGWPSGNLARNSSFSQQQFAHQGNPAVYSMVHMNGSSGHMGQMNMNPMPMSGMPMGPDQKYC DIP protein Q9Y6Q9 original sequence version: 162 DIP protein Q9Y6Q9 original sequence version: 155 DIP protein Q9Y6Q9 original sequence version: 91 732CDF0423161679 smrc2_human SWI/SNF complex subunit SMARCC2 SMARCC2 BAF170 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2 SWI/SNF complex 170 kDa subunit BRG1-associated factor 170 protein protein human Homo sapiens Human MAVRKKDGGPNVKYYEAADTVTQFDNVRLWLGKNYKKYIQAEPPTNKSLSSLVVQLLQFQEEVFGKHVSNAPLTKLPIKCFLDFKAGGSLCHILAAAYKFKSDQGWRRYDFQNPSRMDRNVEMFMTIEKSLVQNNCLSRPNIFLCPEIEPKLLGKLKDIIKRHQGTVTEDKNNASHVVYPVPGNLEEEEWVRPVMKRDKQVLLHWGYYPDSYDTWIPASEIEASVEDAPTPEKPRKVHAKWILDTDTFNEWMNEEDYEVNDDKNPVSRRKKISAKTLTDEVNSPDSDRRDKKGGNYKKRKRSPSPSPTPEAKKKNAKKGPSTPYTKSKRGHREEEQEDLTKDMDEPSPVPNVEEVTLPKTVNTKKDSESAPVKGGTMTDLDEQEDESMETTGKDEDENSTGNKGEQTKNPDLHEDNVTEQTHHIIIPSYAAWFDYNSVHAIERRALPEFFNGKNKSKTPEIYLAYRNFMIDTYRLNPQEYLTSTACRRNLAGDVCAIMRVHAFLEQWGLINYQVDAESRPTPMGPPPTSHFHVLADTPSGLVPLQPKTPQQTSASQQMLNFPDKGKEKPTDMQNFGLRTDMYTKKNVPSKSKAAASATREWTEQETLLLLEALEMYKDDWNKVSEHVGSRTQDECILHFLRLPIEDPYLEDSEASLGPLAYQPIPFSQSGNPVMSTVAFLASVVDPRVASAAAKSALEEFSKMKEEVPTALVEAHVRKVEEAAKVTGKADPAFGLESSGIAGTTSDEPERIEESGNDEARVEGQATDEKKEPKEPREGGGAIEEEAKEKTSEAPKKDEEKGKEGDSEKESEKSDGDPIVDPEKEKEPKEGQEEVLKEVVESEGERKTKVERDIGEGNLSTAAAAALAAAAVKAKHLAAVEERKIKSLVALLVETQMKKLEIKLRHFEELETIMDREREALEYQRQQLLADRQAFHMEQLKYAEMRARQQHFQQMHQQQQQPPPALPPGSQPIPPTGAAGPPAVHGLAVAPASVVPAPAGSGAPPGSLGPSEQIGQAGSTAGPQQQQPAGAPQPGAVPPGVPPPGPHGPSPFPNQQTPPSMMPGAVPGSGHPGVAGNAPLGLPFGMPPPPPPPAPSIIPFGSLADSISINLPAPPNLHGHHHHLPFAPGTLPPPNLPVSMANPLHPNLPATTTMPSSLPLGPGLGSAAAQSPAIVAAVQGNLLPSASPLPDPGTPLPPDPTAPSPGTVTPVPPPQ DIP protein Q8TAQ2 original sequence version: null DIP protein Q8TAQ2 original sequence version: 76 DIP protein Q8TAQ2 original sequence version: 95 EEFC1042A9296320 smce1_human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1 BRG1-associated factor 57 SMARCE1 BAF57 protein protein human Homo sapiens Human MSKRPSYAPPPTPAPATQMPSTPGFVGYNPYSHLAYNNYRLGGNPGTNSRVTASSGITIPKPPKPPDKPLMPYMRYSRKVWDQVKASNPDLKLWEIGKIIGGMWRDLTDEEKQEYLNEYEAEKIEYNESMKAYHNSPAYLAYINAKSRAEAALEEESRQRQSRMEKGEPYMSIQPAEDPDDYDDGFSMKHTATARFQRNHRLISEILSESVVPDVRSVVTTARMQVLKRQVQSLMVHQRKLEAELLQIEERHQEKKRKFLESTDSFNNELKRLCGLKVEVDMEKIAAEIAQAEEQARKRQEEREKEAAEQAERSQSSIVPEEEQAANKGEEKKDDENIPMETEETHLEETTESQQNGEEGTSTPEDKESGQEGVDSMAEEGTSDSNTGSESNSATVEEPPTDPIPEDEKKE DIP protein Q969G3 original sequence version: 86 DIP protein Q969G3 original sequence version: 78 DIP protein Q969G3 original sequence version: 97 6F1C1B7917BAD506 prgr_human Progesterone receptor PGR NR3C3 Nuclear receptor subfamily 3 group C member 3 protein protein human Homo sapiens Human MTELKAKGPRAPHVAGGPPSPEVGSPLLCRPAAGPFPGSQTSDTLPEVSAIPISLDGLLFPRPCQGQDPSDEKTQDQQSLSDVEGAYSRAEATRGAGGSSSSPPEKDSGLLDSVLDTLLAPSGPGQSQPSPPACEVTSSWCLFGPELPEDPPAAPATQRVLSPLMSRSGCKVGDSSGTAAAHKVLPRGLSPARQLLLPASESPHWSGAPVKPSPQAAAVEVEEEDGSESEESAGPLLKGKPRALGGAAAGGGAAAVPPGAAAGGVALVPKEDSRFSAPRVALVEQDAPMAPGRSPLATTVMDFIHVPILPLNHALLAARTRQLLEDESYDGGAGAASAFAPPRSSPCASSTPVAVGDFPDCAYPPDAEPKDDAYPLYSDFQPPALKIKEEEEGAEASARSPRSYLVAGANPAAFPDFPLGPPPPLPPRATPSRPGEAAVTAAPASASVSSASSSGSTLECILYKAEGAPPQQGPFAPPPCKAPGASGCLLPRDGLPSTSASAAAAGAAPALYPALGLNGLPQLGYQAAVLKEGLPQVYPPYLNYLRPDSEASQSPQYSFESLPQKICLICGDEASGCHYGVLTCGSCKVFFKRAMEGQHNYLCAGRNDCIVDKIRRKNCPACRLRKCCQAGMVLGGRKFKKFNKVRVVRALDAVALPQPVGVPNESQALSQRFTFSPGQDIQLIPPLINLLMSIEPDVIYAGHDNTKPDTSSSLLTSLNQLGERQLLSVVKWSKSLPGFRNLHIDDQITLIQYSWMSLMVFGLGWRSYKHVSGQMLYFAPDLILNEQRMKESSFYSLCLTMWQIPQEFVKLQVSQEEFLCMKVLLLLNTIPLEGLRSQTQFEEMRSSYIRELIKAIGLRQKGVVSSSQRFYQLTKLLDNLHDLVKQLHLYCLNTFIQSRALSVEFPEMMSEVIAAQLPKILAGMVKPLLFHKK DIP protein P06401 original sequence version: 196 DIP protein P06401-1 original sequence version: 186 DIP protein P06401-1 original sequence version: 192 80452E54FF3A0454 smca4_human Transcription activator BRG1 SMARCA4 BRG1 SNF2B SNF2L4 ATP-dependent helicase SMARCA4 SNF2-beta BAF190A Mitotic growth and transcription activator Protein BRG-1 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 Protein brahma homolog 1 BRG1-associated factor 190A protein protein human Homo sapiens Human MSTPDPPLGGTPRPGPSPGPGPSPGAMLGPSPGPSPGSAHSMMGPSPGPPSAGHPIPTQGPGGYPQDNMHQMHKPMESMHEKGMSDDPRYNQMKGMGMRSGGHAGMGPPPSPMDQHSQGYPSPLGGSEHASSPVPASGPSSGPQMSSGPGGAPLDGADPQALGQQNRGPTPFNQNQLHQLRAQIMAYKMLARGQPLPDHLQMAVQGKRPMPGMQQQMPTLPPPSVSATGPGPGPGPGPGPGPGPAPPNYSRPHGMGGPNMPPPGPSGVPPGMPGQPPGGPPKPWPEGPMANAAAPTSTPQKLIPPQPTGRPSPAPPAVPPAASPVMPPQTQSPGQPAQPAPMVPLHQKQSRITPIQKPRGLDPVEILQEREYRLQARIAHRIQELENLPGSLAGDLRTKATIELKALRLLNFQRQLRQEVVVCMRRDTALETALNAKAYKRSKRQSLREARITEKLEKQQKIEQERKRRQKHQEYLNSILQHAKDFKEYHRSVTGKIQKLTKAVATYHANTEREQKKENERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAYLLQQTDEYVANLTELVRQHKAAQVAKEKKKKKKKKKAENAEGQTPAIGPDGEPLDETSQMSDLPVKVIHVESGKILTGTDAPKAGQLEAWLEMNPGYEVAPRSDSEESGSEEEEEEEEEEQPQAAQPPTLPVEEKKKIPDPDSDDVSEVDARHIIENAKQDVDDEYGVSQALARGLQSYYAVAHAVTERVDKQSALMVNGVLKQYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTIALITYLMEHKRINGPFLIIVPLSTLSNWAYEFDKWAPSVVKVSYKGSPAARRAFVPQLRSGKFNVLLTTYEYIIKDKHILAKIRWKYMIVDEGHRMKNHHCKLTQVLNTHYVAPRRLLLTGTPLQNKLPELWALLNFLLPTIFKSCSTFEQWFNAPFAMTGEKVDLNEEETILIIRRLHKVLRPFLLRRLKKEVEAQLPEKVEYVIKCDMSALQRVLYRHMQAKGVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESFSEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAYRGFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSDWNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHERRAFLQAILEHEEQDESRHCSTGSGSASFAHTAPPPAGVNPDLEEPPLKEEDEVPDDETVNQMIARHEEEFDLFMRMDLDRRREEARNPKRKPRLMEEDELPSWIIKDDAEVERLTCEEEEEKMFGRGSRHRKEVDYSDSLTEKQWLKAIEEGTLEEIEEEVRQKKSSRKRKRDSDAGSSTPTTSTRSRDKDDESKKQKKRGRPPAEKLSPNPPNLTKKMKKIVDAVIKYKDSSSGRQLSEVFIQLPSRKELPEYYELIRKPVDFKKIKERIRNHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFTSVRQKIEKEDDSEGEESEEEEEGEEEGSESESRSVKVKIKLGRKEKAQDRLKGGRRRPSRGSRAKPVVSDDDSEEEQEEDRSGSGSEED DIP protein P51532 original sequence version: 125 DIP protein P51532 original sequence version: 115 DIP protein P51532 original sequence version: 154 DIP protein P51532 original sequence version: 136 DIP protein P51532-1 original sequence version: 136 ABDA5EDBF10D7D28 smrc1_human SWI/SNF complex subunit SMARCC1 SMARCC1 BAF155 SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1 SWI/SNF complex 155 kDa subunit BRG1-associated factor 155 protein protein human Homo sapiens Human MAAAAGGGGPGTAVGATGSGIAAAAAGLAVYRRKDGGPATKFWESPETVSQLDSVRVWLGKHYKKYVHADAPTNKTLAGLVVQLLQFQEDAFGKHVTNPAFTKLPAKCFMDFKAGGALCHILGAAYKYKNEQGWRRFDLQNPSRMDRNVEMFMNIEKTLVQNNCLTRPNIYLIPDIDLKLANKLKDIIKRHQGTFTDEKSKASHHIYPYSSSQDDEEWLRPVMRKEKQVLVHWGFYPDSYDTWVHSNDVDAEIEDPPIPEKPWKVHVKWILDTDIFNEWMNEEDYEVDENRKPVSFRQRISTKNEEPVRSPERRDRKASANARKRKHSPSPPPPTPTESRKKSGKKGQASLYGKRRSQKEEDEQEDLTKDMEDPTPVPNIEEVVLPKNVNLKKDSENTPVKGGTVADLDEQDEETVTAGGKEDEDPAKGDQSRSVDLGEDNVTEQTNHIIIPSYASWFDYNCIHVIERRALPEFFNGKNKSKTPEIYLAYRNFMIDTYRLNPQEYLTSTACRRNLTGDVCAVMRVHAFLEQWGLVNYQVDPESRPMAMGPPPTPHFNVLADTPSGLVPLHLRSPQVPAAQQMLNFPEKNKEKPVDLQNFGLRTDIYSKKTLAKSKGASAGREWTEQETLLLLEALEMYKDDWNKVSEHVGSRTQDECILHFLRLPIEDPYLENSDASLGPLAYQPVPFSQSGNPVMSTVAFLASVVDPRVASAAAKAALEEFSRVREEVPLELVEAHVKKVQEAARASGKVDPTYGLESSCIAGTGPDEPEKLEGAEEEKMEADPDGQQPEKAENKVENETDEGDKAQDGENEKNSEKEQDSEVSEDTKSEEKETEENKELTDTCKERESDTGKKKVEHEISEGNVATAAAAALASAATKAKHLAAVEERKIKSLVALLVETQMKKLEIKLRHFEELETIMDREKEALEQQRQQLLTERQNFHMEQLKYAELRARQQMEQQQHGQNPQQAHQHSGGPGLAPLGAAGHPGMMPHQQPPPYPLMHHQMPPPHPPQPGQIPGPGSMMPGQHMPGRMIPTVAANIHPSGSGPTPPGMPPMPGNILGPRVPLTAPNGMYPPPPQQQPPPPPPADGVPPPPAPGPPASAAP DIP protein Q92922 original sequence version: 99 EDA6FF5B0472AEA9 ncoa1_human Nuclear receptor coactivator 1 Steroid receptor coactivator 1 RIP160 Protein Hin-2 Renal carcinoma antigen NY-REN-52 NCOA1 SRC1 BHLHE74 Class E basic helix-loop-helix protein 74 protein protein human Homo sapiens Human MSGLGDSSSDPANPDSHKRKGSPCDTLASSTEKRRREQENKYLEELAELLSANISDIDSLSVKPDKCKILKKTVDQIQLMKRMEQEKSTTDDDVQKSDISSSSQGVIEKESLGPLLLEALDGFFFVVNCEGRIVFVSENVTSYLGYNQEELMNTSVYSILHVGDHAEFVKNLLPKSLVNGVPWPQEATRRNSHTFNCRMLIHPPDEPGTENQEACQRYEVMQCFTVSQPKSIQEDGEDFQSCLICIARRLPRPPAITGVESFMTKQDTTGKIISIDTSSLRAAGRTGWEDLVRKCIYAFFQPQGREPSYARQLFQEVMTRGTASSPSYRFILNDGTMLSAHTKCKLCYPQSPDMQPFIMGIHIIDREHSGLSPQDDTNSGMSIPRVNPSVNPSISPAHGVARSSTLPPSNSNMVSTRINRQQSSDLHSSSHSNSSNSQGSFGCSPGSQIVANVALNQGQASSQSSNPSLNLNNSPMEGTGISLAQFMSPRRQVTSGLATRPRMPNNSFPPNISTLSSPVGMTSSACNNNNRSYSNIPVTSLQGMNEGPNNSVGFSASSPVLRQMSSQNSPSRLNIQPAKAESKDNKEIASILNEMIQSDNSSSDGKPLDSGLLHNNDRLSDGDSKYSQTSHKLVQLLTTTAEQQLRHADIDTSCKDVLSCTGTSNSASANSSGGSCPSSHSSLTERHKILHRLLQEGSPSDITTLSVEPDKKDSASTSVSVTGQVQGNSSIKLELDASKKKESKDHQLLRYLLDKDEKDLRSTPNLSLDDVKVKVEKKEQMDPCNTNPTPMTKPTPEEIKLEAQSQFTADLDQFDQLLPTLEKAAQLPGLCETDRMDGAVTSVTIKSEILPASLQSATARPTSRLNRLPELELEAIDNQFGQPGTGDQIPWTNNTVTAINQSKSEDQCISSQLDELLCPPTTVEGRNDEKALLEQLVSFLSGKDETELAELDRALGIDKLVQGGGLDVLSERFPPQQATPPLIMEERPNLYSQPYSSPSPTANLPSPFQGMVRQKPSLGTMPVQVTPPRGAFSPGMGMQPRQTLNRPPAAPNQLRLQLQQRLQGQQQLIHQNRQAILNQFAATAPVGINMRSGMQQQITPQPPLNAQMLAQRQRELYSQQHRQRQLIQQQRAMLMRQQSFGNNLPPSSGLPVQMGNPRLPQGAPQQFPYPPNYGTNPGTPPASTSPFSQLAANPEASLANRNSMVSRGMTGNIGGQFGTGINPQMQQNVFQYPGAGMVPQGEANFAPSLSPGSSMVPMPIPPPQSSLLQQTPPASGYQSPDMKAWQQGAIGNNNVFSQAVQNQPTPAQPGVYNNMSITVSMAGGNTNVQNMNPMMAQMQMSSLQMPGMNTVCPEQINDPALRHTGLYCNQLSSTDLLKTEADGTQQVQQVQVFADVQCTVNLVGGDPYLNQPGPLGTQKPTSGPQTPQAQQKSLLQQLLTE DIP protein Q15788-1 original sequence version: 60 DIP protein Q15788-1 original sequence version: 62 DIP protein Q15788 original sequence version: 88 DIP protein Q15788 original sequence version: 107 DIP protein Q15788 original sequence version: 96 25EF6F389489121E smrd1_human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1 SMARCD1 BAF60A SWI/SNF complex 60 kDa subunit 60 kDa BRG-1/Brm-associated factor subunit A BRG1-associated factor 60A protein protein human Homo sapiens Human MAARAGFQSVAPSGGAGASGGAGAAAALGPGGTPGPPVRMGPAPGQGLYRSPMPGAAYPRPGMLPGSRMTPQGPSMGPPGYGGNPSVRPGLAQSGMDQSRKRPAPQQIQQVQQQAVQNRNHNAKKKKMADKILPQRIRELVPESQAYMDLLAFERKLDQTIMRKRLDIQEALKRPIKQKRKLRIFISNTFNPAKSDAEDGEGTVASWELRVEGRLLEDSALSKYDATKQKRKFSSFFKSLVIELDKDLYGPDNHLVEWHRTATTQETDGFQVKRPGDVNVRCTVLLMLDYQPPQFKLDPRLARLLGIHTQTRPVIIQALWQYIKTHKLQDPHEREFVICDKYLQQIFESQRMKFSEIPQRLHALLMPPEPIIINHVISVDPNDQKKTACYDIDVEVDDTLKTQMNSFLLSTASQQEIATLDNKIHETIETINQLKTQREFMLSFARDPQGFINDWLQSQCRDLKTMTDVVGNPEEERRAEFYFQPWAQEAVCRYFYSKVQQRRQELEQALGIRNT DIP protein Q96GM5 original sequence version: null DIP protein Q96GM5 original sequence version: 75 E683AA1E345DA400 nr3c1-smarcc1 2 6 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour 13 unspecified role unspecified role prey prey human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour physical association physical association 1B Consistent with the constitutive activation seen in these cells, the GR1-556 protein interacted with the steroid receptor coactivator SRC-1 in the absence of hormone (Fig. 1B). In addition, we also observed GR1-556 interactions with chromatin-remodeling proteins BRG1 and BAF155 in the absence of hormone (Fig.1B) nr3c1-smarca4 2 6 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour 12 unspecified role unspecified role prey prey human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour physical association physical association 1B Consistent with the constitutive activation seen in these cells, the GR1-556 protein interacted with the steroid receptor coactivator SRC-1 in the absence of hormone (Fig. 1B). In addition, we also observed GR1-556 interactions with chromatin-remodeling proteins BRG1 and BAF155 in the absence of hormone (Fig.1B) nr3c1-ncoa1 2 14 unspecified role unspecified role prey prey human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour 6 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour physical association physical association 1B Consistent with the constitutive activation seen in these cells, the GR1-556 protein interacted with the steroid receptor coactivator SRC-1 in the absence of hormone (Fig. 1B). In addition, we also observed GR1-556 interactions with chromatin-remodeling proteins BRG1 and BAF155 in the absence of hormone (Fig.1B) nr3c1-nr3c1 2 6 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour 6 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain human-t47d human breast cancer tumour cell line-epithelial cells t47d Epithelial cells derived from human breast tumour physical association physical association 1B Consistent with the constitutive activation seen in these cells, the GR1-556 protein interacted with the steroid receptor coactivator SRC-1 in the absence of hormone (Fig. 1B). In addition, we also observed GR1-556 interactions with chromatin-remodeling proteins BRG1 and BAF155 in the absence of hormone (Fig.1B) nr3c1-ncoa1-1 4 14 unspecified role unspecified role prey prey in vitro In vitro 6 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 1C In agreement with the in vivo coimmunoprecipitation results (Fig. 1B), the GR1-556 directly interacts with coactivators SRC-1 and SRC-3, but not histone deacetylase protein 1 (HDAC1), which is often found as part of a corepressor complex (Fig. 1C). Similar pull-down conditions were then applied to test whether the GR1-556 interacts with core catalytic BRG1 complex, BRG1, BAF170, and BAF155 (Fig. 1C) nr3c1-ncoa3 4 6 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role prey prey in vitro In vitro physical association physical association 1C In agreement with the in vivo coimmunoprecipitation results (Fig. 1B), the GR1-556 directly interacts with coactivators SRC-1 and SRC-3, but not histone deacetylase protein 1 (HDAC1), which is often found as part of a corepressor complex (Fig. 1C). Similar pull-down conditions were then applied to test whether the GR1-556 interacts with core catalytic BRG1 complex, BRG1, BAF170, and BAF155 (Fig. 1C) nr3c1-smarcd1 4 6 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 15 unspecified role unspecified role prey prey in vitro In vitro physical association physical association 1C In agreement with the in vivo coimmunoprecipitation results (Fig. 1B), the GR1-556 directly interacts with coactivators SRC-1 and SRC-3, but not histone deacetylase protein 1 (HDAC1), which is often found as part of a corepressor complex (Fig. 1C). Similar pull-down conditions were then applied to test whether the GR1-556 interacts with core catalytic BRG1 complex, BRG1, BAF170, and BAF155 (Fig. 1C) nr3c1-smarce1 4 6 unspecified role unspecified role bait bait n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 10 unspecified role unspecified role prey prey in vitro In vitro physical association physical association 1C In agreement with the in vivo coimmunoprecipitation results (Fig. 1B), the GR1-556 directly interacts with coactivators SRC-1 and SRC-3, but not histone deacetylase protein 1 (HDAC1), which is often found as part of a corepressor complex (Fig. 1C). Similar pull-down conditions were then applied to test whether the GR1-556 interacts with core catalytic BRG1 complex, BRG1, BAF170, and BAF155 (Fig. 1C) smarcd1-nr3c1un 4 6 unspecified role unspecified role prey prey n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain arg488gln binding region binding-associated region binding region mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 15 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association 2A, 2B The results demonstrate that GR1-556 is similar to BRG1 in that it interacts with the N terminus of BAF60a but not the C terminus (Fig. 2A) The N-terminal of human SMARCD1 interacts with the n-terminal of rat GR/NR3C1. There was no affect on the interaction by addition of the hormone dexamethasone. This interaction is abolished when arg 488 is mutated to gln in the rat GR/NR3C1 As shown, N-terminal BAF60a also interacts with progesterone receptor b (PRb), ER alpha (ERalpha, and farnesoid receptor (FXR), and minimally with peroxisome proliferator-activated receptors gamma (PPARgamma), suggesting conservation of mechanism with the GR (Fig. 2B). In agreement with our prediction, the GR(R488Q) mutant failed to interact with BAF60a in our assay (Fig. 2B). smarcd1-smarca4 4 12 unspecified role unspecified role prey prey in vitro In vitro 15 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association 2A The N-terminal BAF60a interacts strongly only with BRG1, interacts to a lesser degree with BAF155, and interacts not at all with BAF170, whereas the C-terminal BAF60a interacts strongly with BAF170 and BAF155 but not with BRG1 (Fig. 2A). smarcd1-smarcc1 4 13 unspecified role unspecified role prey prey in vitro In vitro 15 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain c-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association 2A The N-terminal BAF60a interacts strongly only with BRG1, interacts to a lesser degree with BAF155, and interacts not at all with BAF170, whereas the C-terminal BAF60a interacts strongly with BAF170 and BAF155 but not with BRG1 (Fig. 2A). smarcd1-smarcc2 4 9 unspecified role unspecified role prey prey in vitro In vitro 15 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association 2A The N-terminal BAF60a interacts strongly only with BRG1, interacts to a lesser degree with BAF155, and interacts not at all with BAF170, whereas the C-terminal BAF60a interacts strongly with BAF170 and BAF155 but not with BRG1 (Fig. 2A). smarcd1-pgr 4 11 unspecified role unspecified role prey prey in vitro In vitro 15 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association 2B As shown, N-terminal BAF60a also interacts with progesterone receptor b (PRb), ER alpha (ERalpha), and farnesoid receptor (FXR), and minimally with peroxisome proliferator-activated receptors gamma (PPARgamma), suggesting conservation of mechanism with the GR (Fig. 2B). The addition of the hormone R5020 had no effect on the interaction between the n-terminal of smarcd1 and the progesterone receptor-b, pgr smarcd1-esr1 4 15 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role prey prey in vitro In vitro physical association physical association 2B As shown, N-terminal BAF60a also interacts with progesterone receptor b (PRb), ER alpha (ERalpha), and farnesoid receptor (FXR), and minimally with peroxisome proliferator-activated receptors gamma (PPARgamma), suggesting conservation of mechanism with the GR (Fig. 2B). The addition of the hormone beta-estradiol had no effect on the interaction between the n-terminal of smarcd1 and the estrogen receptor-alpha, er-alpha smarcd1-nr1h4 4 5 unspecified role unspecified role prey prey in vitro In vitro 15 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association 2B As shown, N-terminal BAF60a also interacts with progesterone receptor b (PRb), ER alpha (ERalpha), and farnesoid receptor (FXR), and minimally with peroxisome proliferator-activated receptors gamma (PPARgamma), suggesting conservation of mechanism with the GR (Fig. 2B). The addition of the hormone chenodeoxycholic acid had no effect on the interaction between the n-terminal of smarcd1 and the farnesoid receptor, fxr smarca4-smarcd1 3 15 unspecified role unspecified role prey prey human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference 12 unspecified role unspecified role bait bait human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference physical association physical association 6A In order to determine how BAF60a4-140 inhibits GR-activated chromatin remodeling, we investigated the protein-protein interactions with the BRG1 complex in both UL3 and 60N.17 cells. The BRG1 complex in UL3 and 60N.17 cells treated with vehicle or hormone was immunoprecipitated with an antibody against BRG1. In agreement with earlier studies, the GR shows a hormone-dependent interaction with BRG1 in UL3 cells such that the GR is enriched in BRG1 complex upon hormone treatment (Fig. 6A, compare lanes 5 and 6). In contrast, hormone treatment does not enrich GR in the BRG1 complex in 60N.17 cells (Fig. 6A, compare lanes 7 and 8). The interaction between SMARCA4 and SMARCD1 is unaffected by the presence of the hormone dexamethasone. smarca4-smarca4 3 12 unspecified role unspecified role prey prey human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference 12 unspecified role unspecified role bait bait human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference physical association physical association 6A In order to determine how BAF60a4-140 inhibits GR-activated chromatin remodeling, we investigated the protein-protein interactions with the BRG1 complex in both UL3 and 60N.17 cells. The BRG1 complex in UL3 and 60N.17 cells treated with vehicle or hormone was immunoprecipitated with an antibody against BRG1. In agreement with earlier studies, the GR shows a hormone-dependent interaction with BRG1 in UL3 cells such that the GR is enriched in BRG1 complex upon hormone treatment (Fig. 6A, compare lanes 5 and 6). In contrast, hormone treatment does not enrich GR in the BRG1 complex in 60N.17 cells (Fig. 6A, compare lanes 7 and 8). The interaction of SMARCA4 with itself was unaffected by the presence of the hormone dexamethasone smarca4-smarcc1 3 13 unspecified role unspecified role prey prey human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference 12 unspecified role unspecified role bait bait human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference physical association physical association 6A In order to determine how BAF60a4-140 inhibits GR-activated chromatin remodeling, we investigated the protein-protein interactions with the BRG1 complex in both UL3 and 60N.17 cells. The BRG1 complex in UL3 and 60N.17 cells treated with vehicle or hormone was immunoprecipitated with an antibody against BRG1. In agreement with earlier studies, the GR shows a hormone-dependent interaction with BRG1 in UL3 cells such that the GR is enriched in BRG1 complex upon hormone treatment (Fig. 6A, compare lanes 5 and 6). In contrast, hormone treatment does not enrich GR in the BRG1 complex in 60N.17 cells (Fig. 6A, compare lanes 7 and 8). The interaction of SMARCA4 with SMARCC1 was unaffected by the presence of the hormone dexamethasone smarca4-nr3c1-1 3 6 unspecified role unspecified role prey prey human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference 12 unspecified role unspecified role bait bait human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference physical association physical association 6A In order to determine how BAF60a4-140 inhibits GR-activated chromatin remodeling, we investigated the protein-protein interactions with the BRG1 complex in both UL3 and 60N.17 cells. The BRG1 complex in UL3 and 60N.17 cells treated with vehicle or hormone was immunoprecipitated with an antibody against BRG1. In agreement with earlier studies, the GR shows a hormone-dependent interaction with BRG1 in UL3 cells such that the GR is enriched in BRG1 complex upon hormone treatment (Fig. 6A, compare lanes 5 and 6). In contrast, hormone treatment does not enrich GR in the BRG1 complex in 60N.17 cells (Fig. 6A, compare lanes 7 and 8). GR/NR3C1 shows a hormone-dependent interaction with BRG1/SMARCA4 in UL3 cells such that the GR/NR3C1 is enriched in BRG1 complex upon treatment with dexamethasone. In contrast, hormone treatment does not enrich GR/NR3C1 in the BRG1 complex in 60N.17 cells. smarca4-nr3c1-2 1 12 unspecified role unspecified role bait bait human-sw13 human sw13 cells from bone sw13 SW1353 Species: human, Caucasian female 72 years old; Tissue: bone; Tumor: chondrosarcoma 6 unspecified role unspecified role prey prey P06536:p.Arg488Gln mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 16:27:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg488gln' Tue Jun 30 16:41:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg488Gln' n-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-sw13 human sw13 cells from bone sw13 SW1353 Species: human, Caucasian female 72 years old; Tissue: bone; Tumor: chondrosarcoma physical association physical association 4B We next directly investigated BRG1 complex interactions with the GR mutants by coimmunoprecipitation assays. As the results show, BRG1 interacts with GRwt in addition to GR(R488Q) and GR1-556 mutants (Fig. 4B). BRG1/SMARCA4 interacts with NR3C1/GRwt in addition to NR3C1/GR(R488Q) and NR3C1/GR1-556 mutants.