IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Menin associates with FANCD2, a protein involved in repair of DNA damage. Menin associates with FANCD2, a protein involved in repair of DNA damage. Cancer Res. (0008-5472) 2003 imex curation Jin S., Mao H., Schnepp RW., Sykes SM., Silva AC., D'Andrea AD., Hua X. Cancer - Interactions investigated in the context of cancer Chromatin - Epigenetic interactions resulting in chromatin modulation Cells exposed to 2500 rads of gamma irradiation huax@mail.med.upenn.edu 14-Nov-2008: Contacted by JYOTI. orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jin S., Mao H., Schnepp RW., Sykes SM., Silva AC., D'Andrea AD., Hua X. Cancer - Interactions investigated in the context of cancer Cells exposed to 2500 rads of gamma irradiation huax@mail.med.upenn.edu Accepted 2008-NOV-13 by ORCHARD. 14-Nov-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2003 orchard Only protein-protein interactions imex curation Menin associates with FANCD2, a protein involved in repair of DNA damage. Menin associates with FANCD2, a protein involved in repair of DNA damage. Cancer Res. (0008-5472) 2003 imex curation Jin S., Mao H., Schnepp RW., Sykes SM., Silva AC., D'Andrea AD., Hua X. Cancer - Interactions investigated in the context of cancer Chromatin - Epigenetic interactions resulting in chromatin modulation Cells exposed to 2500 rads of gamma irradiation huax@mail.med.upenn.edu 14-Nov-2008: Contacted by JYOTI. orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Jin S., Mao H., Schnepp RW., Sykes SM., Silva AC., D'Andrea AD., Hua X. Cancer - Interactions investigated in the context of cancer Cells exposed to 2500 rads of gamma irradiation huax@mail.med.upenn.edu Accepted 2008-NOV-13 by ORCHARD. 14-Nov-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2003 orchard Only protein-protein interactions imex curation Menin associates with FANCD2, a protein involved in repair of DNA damage. Menin associates with FANCD2, a protein involved in repair of DNA damage. Cancer Res. (0008-5472) 2003 imex curation Jin S., Mao H., Schnepp RW., Sykes SM., Silva AC., D'Andrea AD., Hua X. Cancer - Interactions investigated in the context of cancer Chromatin - Epigenetic interactions resulting in chromatin modulation Cells exposed to 2500 rads of gamma irradiation huax@mail.med.upenn.edu 14-Nov-2008: Contacted by JYOTI. orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jin S., Mao H., Schnepp RW., Sykes SM., Silva AC., D'Andrea AD., Hua X. Cancer - Interactions investigated in the context of cancer Cells exposed to 2500 rads of gamma irradiation huax@mail.med.upenn.edu Accepted 2008-NOV-13 by ORCHARD. 14-Nov-2008: Contacted by JYOTI. Cancer Res. (0008-5472) 2003 orchard Only protein-protein interactions imex curation Menin associates with FANCD2, a protein involved in repair of DNA damage. Menin associates with FANCD2, a protein involved in repair of DNA damage. Cancer Res. (0008-5472) 2003 imex curation Jin S., Mao H., Schnepp RW., Sykes SM., Silva AC., D'Andrea AD., Hua X. Cancer - Interactions investigated in the context of cancer Chromatin - Epigenetic interactions resulting in chromatin modulation Cells exposed to 2500 rads of gamma irradiation huax@mail.med.upenn.edu 14-Nov-2008: Contacted by JYOTI. orchard Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Jin S., Mao H., Schnepp RW., Sykes SM., Silva AC., D'Andrea AD., Hua X. Cancer - Interactions investigated in the context of cancer Cells exposed to 2500 rads of gamma irradiation huax@mail.med.upenn.edu Accepted 2008-NOV-13 by ORCHARD. Cancer Res. (0008-5472) 2003 orchard Only protein-protein interactions imex curation men1_human Menin MEN1 SCG2 protein protein human Homo sapiens Human MGLKAAQKTLFPLRSIDDVVRLFAAELGREEPDLVLLSLVLGFVEHFLAVNRVIPTNVPELTFQPSPAPDPPGGLTYFPVADLSIIAALYARFTAQIRGAVDLSLYPREGGVSSRELVKKVSDVIWNSLSRSYFKDRAHIQSLFSFITGWSPVGTKLDSSGVAFAVVGACQALGLRDVHLALSEDHAWVVFGPNGEQTAEVTWHGKGNEDRRGQTVNAGVAERSWLYLKGSYMRCDRKMEVAFMVCAINPSIDLHTDSLELLQLQQKLLWLLYDLGHLERYPMALGNLADLEELEPTPGRPDPLTLYHKGIASAKTYYRDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQDYNYCREDEEIYKEFFEVANDVIPNLLKEAASLLEAGEERPGEQSQGTQSQGSALQDPECFAHLLRFYDGICKWEEGSPTPVLHVGWATFLVQSLGRFEGQVRQKVRIVSREAEAAEAEEPWGEEAREGRRRGPRRESKPEEPPPPKKPALDKGLGTGQGAVSGPPRKPPGTVAGTARGPEGGSTAQVPAPTASPPPEGPVLTFQSEKMKGMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL DIP protein O00255 original sequence version: 100 DIP protein O00255 original sequence version: 131 DDDF850EA5AB77B4 facd2_human Fanconi anemia group D2 protein FANCD2 FACD protein protein human Homo sapiens Human MVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLLKISGIILKTGESQNQLAVDQIAFQKKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDEEASMGASYSKSLIKLLLGIDILQPAIIKTLFEKLPEYFFENKNSDEINIPRLIVSQLKWLDRVVDGKDLTTKIMQLISIAPENLQHDIITSLPEILGDSQHADVGKELSDLLIENTSLTVPILDVLSSLRLDPNFLLKVRQLVMDKLSSIRLEDLPVIIKFILHSVTAMDTLEVISELREKLDLQHCVLPSRLQASQVKLKSKGRASSSGNQESSGQSCIILLFDVIKSAIRYEKTISEAWIKAIENTASVSEHKVFDLVMLFIIYSTNTQTKKYIDRVLRNKIRSGCIQEQLLQSTFSVHYLVLKDMCSSILSLAQSLLHSLDQSIISFGSLLYKYAFKFFDTYCQQEVVGALVTHICSGNEAEVDTALDVLLELVVLNPSAMMMNAVFVKGILDYLDNISPQQIRKLFYVLSTLAFSKQNEASSHIQDDMHLVIRKQLSSTVFKYKLIGIIGAVTMAGIMAADRSESPSLTQERANLSDEQCTQVTSLLQLVHSCSEQSPQASALYYDEFANLIQHEKLDPKALEWVGHTICNDFQDAFVVDSCVVPEGDFPFPVKALYGLEEYDTQDGIAINLLPLLFSQDFAKDGGPVTSQESGQKLVSPLCLAPYFRLLRLCVERQHNGNLEEIDGLLDCPIFLTDLEPGEKLESMSAKERSFMCSLIFLTLNWFREIVNAFCQETSPEMKGKVLTRLKHIVELQIILEKYLAVTPDYVPPLGNFDVETLDITPHTVTAISAKIRKKGKIERKQKTDGSKTSSSDTLSEEKNSECDPTPSHRGQLNKEFTGKEEKTSLLLHNSHAFFRELDIEVFSILHCGLVTKFILDTEMHTEATEVVQLGPPELLFLLEDLSQKLESMLTPPIARRVPFLKNKGSRNIGFSHLQQRSAQEIVHCVFQLLTPMCNHLENIHNYFQCLAAENHGVVDGPGVKVQEYHIMSSCYQRLLQIFHGLFAWSGFSQPENQNLLYSALHVLSSRLKQGEHSQPLEELLSQSVHYLQNFHQSIPSFQCALYLIRLLMVILEKSTASAQNKEKIASLARQFLCRVWPSGDKEKSNISNDQLHALLCIYLEHTESILKAIEEIAGVGVPELINSPKDASSSTFPTLTRHTFVVFFRVMMAELEKTVKKIEPGTAADSQQIHEEKLLYWNMAVRDFSILINLIKVFDSHPVLHVCLKYGRLFVEAFLKQCMPLLDFSFRKHREDVLSLLETFQLDTRLLHHLCGHSKIHQDTRLTQHVPLLKKTLELLVCRVKAMLTLNNCREAFWLGNLKNRDLQGEEIKSQNSQESTADESEDDMSSQASKSKATEDGEEDEVSAGEKEQDSDESYDDSD DIP protein Q9BXW9 original sequence version: 96 DIP protein Q9BXW9 original sequence version: 111 DIP protein Q9BXW9-2 original sequence version: 44 DIP protein Q9BXW9 original sequence version: 93 BF931980ADA67405 menin-fancd2 4 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2 protein fragment, F2, which includes amino acid residues 219�395 of menin, is capable of interacting with FANCD2 (Fig. 2 O00255 transfection menin-fancd2-1 2 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region identified peptide identified peptide certain certain sequence position certain certain certain sequence position certain physical association physical association 1D The tagged menin and its associating proteins were separated by SDS-PAGE and stained with a sensitive silver staining method (Fig. 1B)Citation . A large preparation of the menin-interacting proteins from 100 x 150-mm dishes of 293 cells were separated by SDS-PAGE and stained with a Colloid Coomassie Blue staining kit. The stained protein band was excised from the gel and digested with trypsin for sequence analysis using mass spectrometry. A single peptide sequence, SEDKESLTEDASK, was identified and found to be identical to an internal peptide in a recently cloned gene, FANCD2 (28) . menin-fancd2-2 1 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey physical association physical association 3 menin may be involved in DNA damage repair in cooperation with FANCD2. IR irradiation increased the interaction of Men1 with Fancd 2 t- 4 hours after treatment. menin-fancd2-3 3 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 1C Fig. 1CCitation shows that FANCD2 was specifically immunoprecipitated by the antimenin antibody but not by the control IgG. FANCD2 endogenous