UniProt UniProt http://www.uniprot.org Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. J. Cell. Sci. (0021-9533) 2003 Hori T., Haraguchi T., Hiraoka Y., Kimura H., Fukagawa T. Chromatin - Epigenetic interactions resulting in chromatin modulation Endogenous KNTC2 and NUF2 proteins were deleted from these DT40 cells which contained GFP tagged versions. tfukugawa@lab.nig.ac.jp 30-OCT-2006: Contacted by JYOTI. chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm chicken bursa lymphoma fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined Hori T., Haraguchi T., Hiraoka Y., Kimura H., Fukagawa T. Chromatin - Epigenetic interactions resulting in chromatin modulation Endogenous KNTC2 and NUF2 proteins were deleted from these DT40 cells which contained GFP tagged versions. tfukugawa@lab.nig.ac.jp Accepted 2006-OCT-3 by DAVET. 30-OCT-2006: Contacted by JYOTI. J. Cell. Sci. (0021-9533) 2003 Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. J. Cell. Sci. (0021-9533) 2003 Hori T., Haraguchi T., Hiraoka Y., Kimura H., Fukagawa T. Chromatin - Epigenetic interactions resulting in chromatin modulation Endogenous KNTC2 and NUF2 proteins were deleted from these DT40 cells which contained GFP tagged versions. tfukugawa@lab.nig.ac.jp 30-OCT-2006: Contacted by JYOTI. chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm chicken bursa lymphoma fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Hori T., Haraguchi T., Hiraoka Y., Kimura H., Fukagawa T. Chromatin - Epigenetic interactions resulting in chromatin modulation Endogenous KNTC2 and NUF2 proteins were deleted from these DT40 cells which contained GFP tagged versions. tfukugawa@lab.nig.ac.jp Accepted 2006-OCT-3 by DAVET. 30-OCT-2006: Contacted by JYOTI. J. Cell. Sci. (0021-9533) 2003 Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. Dynamic behavior of Nuf2-Hec1 complex that localizes to the centrosome and centromere and is essential for mitotic progression in vertebrate cells. J. Cell. Sci. (0021-9533) 2003 Hori T., Haraguchi T., Hiraoka Y., Kimura H., Fukagawa T. Chromatin - Epigenetic interactions resulting in chromatin modulation Endogenous KNTC2 and NUF2 proteins were deleted from these DT40 cells which contained GFP tagged versions. tfukugawa@lab.nig.ac.jp 30-OCT-2006: Contacted by JYOTI. chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm chicken bursa lymphoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hori T., Haraguchi T., Hiraoka Y., Kimura H., Fukagawa T. Chromatin - Epigenetic interactions resulting in chromatin modulation Endogenous KNTC2 and NUF2 proteins were deleted from these DT40 cells which contained GFP tagged versions. tfukugawa@lab.nig.ac.jp Accepted 2006-OCT-3 by DAVET. 30-OCT-2006: Contacted by JYOTI. J. Cell. Sci. (0021-9533) 2003 o57392_chick centoromere protein C CENP-C protein protein chick Gallus gallus (Chicken) Chicken MAERLDHLKKYYRARFCGQGGKKINIQPGQNVLKYIQDCFECDDTDVTINSPNATSSSTPLVRNQERTSTQSPRPDAGLTIVVGNRTCHSTVSLPATPAETVRSKGEPCVFCPEPGRNSRIADSIKTDSPVHRGETQVLTFKDVEGLCESPAKLSDPEDDPGVVGSPLLVEEAKTPVQTRTVSIAPSSPSPVKDHNVEIESDCEFLIDESGCLSFKSWFSIPRKNRKSQKDGSAAPVLKSQSSDKEKTADKKGKNMNVQAEVHSEQKTKNVRVQRDLKRTSESHPVSSDSEGKVLKSKMQSSTHAGKSKKDALKGSSKHRKKMSYESEAEQLMLSGLDSETSDGEQKIRRVMPREDLAVPSAEHQQEQMLSSKKNLTPCKYQQSASKAYQRSVHKKQTKQTLANDTVLKSQRKKLKKSGKRNGNKNPEPRRWKSSDSEPGEEGLEREPLESNEMFTSPPHQELQTSVLQKLAKSEKAEKVLHALESVTDVNNKTPLKAVELLQHLVDSMKNSEKKRSSVTSSGKMLKKVHHRAHKRACSITRDSEPQNTTDSDSSSAPEVARKKRRQSAVRASKKPKSNRHQRLQHSPAAEKAMGPESGLVLEHSEKYTSPSKHCEENNESSDNSEDLHCQIKNLLSDEIGRQKIVLPSNTPNVRRTKRIRLKPLEYWRGERVTYTLKPSGRLLISGIAGAEAEPHMKNKLKSRHKKKRAKTRSEVVKCLDIPLADASKPTSIVDPVTNQEVLLECINSGSSHSCFFEDESIKVYKSLNTSDFAAGKLILKPLKEKGHQFVHMDTIAFYVIRGQIITTLHKTSYYLTSGDYFYVPAGNGYNIRNLLNEESVLHFTQLKNDRAPVGAELCSVTNENWSLEGKS D345F53B628C63F6 ndc80_chick Kinetochore protein NDC80 homolog KNTC2 Kinetochore protein Hec1 Kinetochore-associated protein 2 NDC80 HEC1 RCJMB04_4o15 protein protein chick Gallus gallus (Chicken) Chicken MRRSSTTSGSSGRQSTMVLRVQDSNKMGLQTPQTKDRGTFGKLSMSKTTSATSERKVSFFGKRASGAGGSRNSQYGMFGTEKIKDPRPLHDKAFIQQCIKQLCEFLVENGYAHNVTIKSLQSPSVKDFIKIFTFIYGFLCPSYELPDSKFEEEIPRVFKELGYPFPLSKSSMYTVGAPHTWPQIVAALIWLTDCFKLYAAMRENPSSFDDGQNWGGETDDGIVHNKLFMDYVVKCYEHFMKGGDTYEELDAEVQSKLKDLFNVDEFKIEGLAAENKRLHEEIARLEKERESEPDRLVSLRKLRSSFQADVQKYQAYLANLESHTTILDQKMKSVNEEVETTEMEVEAMKQENARLQHIFDNQKYSVADIERINHERNELQQTINKLTKELEAEEHQLWNEELKYARNKEAIETQLAEYHKLARKLKLIPISAENSKGHDFEIHFNPEAGPNCLVKYRTQIKAPLMEIVNQTEEEIRKATQRKMSLEDTLEQVNAMVAEKKSSVKTLKEEAEKLDDLYHQKLKEAEEEEQKCANELELLEKHKQLLESGVNEGLSEATNELHDIQRQYQIVMQTTTEESRKAGDNLNRLLEVITTHVVSIEKYLDEQNSKIDRDYEEFMSEDLLSTLTGILDSYKKKAESI FACFED7A0857F71B nuf2_chick Kinetochore protein Nuf2 Cell division cycle-associated protein 1 NUF2 CDCA1 RCJMB04_29i8 protein protein chick Gallus gallus (Chicken) Chicken MEALTFPRYSPDDIVAYLRSHVLVGAEARNLTKADLFATLKPEVLHMIFIRILQKVYGIRLEHFYMMPVNVDIVYPQIFEGFLPVCNLYIHMERFLPVCRVNDFQMSDVINPKAKRTARFLSGILNFVHFRECRREAYLELQLNYKSAMEKHQQLETANQELEMKLEKLNTVPVEQQAEFKQLSDDIQELEQLLSHDYRRKTAALQEVISQKKSDITERTRKLNELKVTMATLKEEQEQLKSKIVESPEELKNYKELMKETVKKLKKSKQEVIEKYEGYRDLVEVLPSCQLEVQLYQKKMERQAANVERLASVLSEVRNLEDQLESAQIELKKGKTDEMSLKRLVTAKHERLSTAEIRIKKKREDVEQYKHTVFEYFNRVQEKRGVVYDKVTAIQKEIQQMRFKIQQLNESAEEEEMKAKEIYQNLKAGLEKRHDSLIKTAKNYAASREDKIAELQKGLLRVQSPGSSS 5327A06E09028104 nuf2_kntc2_1 3 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 1A kntc2_nuf2_1 3 5 unspecified role unspecified role bait bait c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey physical association physical association kntc2_nuf2_2 1 5 unspecified role unspecified role neutral component neutral component c-terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Fig. 1B nuf2_cenpc_1 2 6 unspecified role unspecified role neutral component neutral component c-terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 2A