UniProt UniProt http://www.uniprot.org SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast. SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast. Mol. Biol. Cell (1059-1524) 2002 imex curation Nakajima R., Masukata H. Chromatin - Epigenetic interactions resulting in chromatin modulation masukata@bio.sci.osakau.ac.jp 22-Jan-2008: Contacted by JYOTI. orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Nakajima R., Masukata H. Chromatin - Epigenetic interactions resulting in chromatin modulation masukata@bio.sci.osakau.ac.jp Accepted 2007-Not a month!-3 by ORCHARD. 22-Jan-2008: Contacted by JYOTI. Mol. Biol. Cell (1059-1524) 2002 orchard Only protein-protein interactions imex curation SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast. SpSld3 is required for loading and maintenance of SpCdc45 on chromatin in DNA replication in fission yeast. Mol. Biol. Cell (1059-1524) 2002 imex curation Nakajima R., Masukata H. Chromatin - Epigenetic interactions resulting in chromatin modulation masukata@bio.sci.osakau.ac.jp 22-Jan-2008: Contacted by JYOTI. orchard Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Nakajima R., Masukata H. Chromatin - Epigenetic interactions resulting in chromatin modulation masukata@bio.sci.osakau.ac.jp Accepted 2007-Not a month!-3 by ORCHARD. Mol. Biol. Cell (1059-1524) 2002 orchard Only protein-protein interactions imex curation sld3_schpo DNA replication regulator sld3 Meiotically up-regulated gene 175 protein sld3 mug175 SPAC24H6.06 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MNNDHASKKSFCIKAPSNWEKSYLEVWPLVTVPRQCICLRWCISKEYHEFTCSSLQFIVIRPAGTSVLLGRVKSSKANQLVGIEHVEGSRYALIFLSEKLDFKSLKVIANHQLTKSSKSLSNVSNKPLGDQLFRSNSLMSPSLLKKELHRIQSDASQANERESQAPHSFVTHDLISSSKDGNSLTHEFANDSVTEMVQDYTPSCSRDVKSLLDHLYNSYFYQLLMTKTPVVFYVKQMVGKTRQLAVEVHNHVEEKALVDELLKFLDNLKSVDDRKSRLLQCFESHLNYKAWHLEFENEAHQYEIKGYRLWLQNILNRENCQITKLDFEREFSQLKLKEYEIRVLLYFEILYLFLKWDPEYARRRANDNSLLDSRDSGKRKSRKKNAKTLNPFETAQLKLEFTFDGLCIRRTIEQNATERSEDLLLKFCKETIVPYYSSKFPRITRNLLEKCNGLDLLPERSHKHRHSAPPRSKLISSKSEAGRALPGNTSGASISNTSSPHSEASISKDYEILKRRRSNSGVHSLTRSDSSFNGFERDTRRRSSDIARIKNREINLPSSSLSKQRNSMHDISTNFPRRNLSFTEKLTMASLQGQSEESVQPKTTSSLSRSKTLSILEGSVSKRSEPSMDSILVQATPRKSSSVITELPDTPIKMNSLDKASACTVENHIVTESPAHKSNKAQLFVCVPTTPVKKKSASP 398512B0ABD4B66E mcm6_schpo DNA replication licensing factor mcm6 mcm6 mis5 SPBC211.04c Minichromosome maintenance protein 6 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSSLASQGNNASTPAYRYGFQTSEVGDRPTVSMPSQPESSAMLEDDGMVKRKPFAAIGEAIPKVIDTTGESVREAFEEFLLSFSDDRVAGGDALPSASQEKYYVQQIHGLAMYEIHTVYVDYKHLTSYNDVLALAIVEQYYRFSPFLLRALQKLIEKFEPEYYRSSLSRENASLSPNFKASDKTFALAFYNLPFRSTIRDLRTDRIGRLTTITGTVTRTSEVRPELAQGTFICEECHTVVSNVEQAFRYTEPTQCPNELCANKRSWRLNISQSSFQDWQKVRIQENSNEIPTGSMPRTLDVILRGDIVERAKAGDKCAFTGILIAVPDVSQLGIPGVKPEAYRDSRNFGGRDADGVTGLKSLGVRDLTYKLSFLACMVQPDDANDKSGADVRGDGSQGIEEQDEFLQSLSQEEIDDLRAMVHSDHIYSRLTNSLAPSVYGHEIIKKGILLQLMGGVHKLTPEGINLRGDLNICIVGDPSTSKSQFLKYVCNFLPRAIYTSGKASSAAGLTAAVVKDEETGDFTIEAGALMLADNGICAIDEFDKMDLSDQVAIHEAMEQQTISIAKAGIQATLNARTSILAAANPIGGRYNRKTTLRNNINMSAPIMSRFDLFFVVLDECNESVDRHLAKHIVDIHRLRDDAMQPEFSTEQLQRYIRYARTFKPKLNTESCAEIVKKYKQLRMDDAQGAGKNSYRITVRQLESMIRLSEAIARANCVDDITPAFVNEAYSLLRQSIIHVERDDIEVEEDDAEAQELENDNTNTTNGNDNVSSEEALQKPKVKITYDKYVSIMNGILQVLRQRSTEGVDGVPAGDLVQSYLELREDQFHTEEDIIYEVGLVRKVLTRLVHESIIMEIQNLTDSAVRLPFEERVFSIHPNCDIDALLSNGDVPN 3A00DB55B2EE08B7 cdc45_schpo Cell division control protein 45 homolog Suppressor of nda4 protein sna41 SPAC17D4.02 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MFIKRSDYASAYLKIKEASVSGGCTVQLFVALDPDALCACKLLSTLLKGDFISHKIRPVSGYRDLEQANKTLLEQNEDIKFIILLNCGTMVDLNNYLVSMEDVSIYVIDSHRPHNLNNIYIENNIFVFDDGDIEEDMNKIHDAWYAFNSHELSDEENSDSSNEREEEVEDDNRSVESYSSSDYQARSRRRFSEETTQRRAEIKEKRKKRKEFASILSEYYEKGSWYGESITNILFAVASMLGREDNDMLWLAIVGLTCLEIHCQSSKKYFNRSYSLLKDEVNRLNPSPLENQIVGRAHGKTPHDQSIRLEDEFRFMLVRHWSLYDSMLHSAYVGSRLHIWSEEGRKRLHKLLAKMGLSLVECKQTYIHMNMDLKKTLKSSLKRFAPFYGLDDVIFHSFTRTYGFKCTLSASDVSYAISALLEMGNTGVLLQSKTVARSPDMTEEEYLEKFENAQNQEWLHNFYDAYDALDDVDSLERALKLAMHLQRAIVRTGITLLEKRAIKTLRSFRFGLINEGPDLKIFMHPLALTKMSLWIAEAINEQEREFGKLRHLPLVLAAFVEEKNRYLIVGTSTSAFTSNEDDDDDDGHGHNRFGVAFQEVANMTSATLQMDCFEASVIECQKSDLGVFLESLSFKTLL AE537DE856AF61B7 sld3-cdc45-1 2 3 unspecified role unspecified role bait bait c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role prey prey association association Fig 3A, 3B SpSld3-FLAG, SpMcm6, and SpCdc45 in the immunoprecipitates were analyzed by immunoblotting. cdc45-sld3-1 1 3 unspecified role unspecified role prey prey c-terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 3A In a reciprocal experiment using an anti-SpCdc45 antibody, SpSld3-FLAG coprecipitated with SpCdc45 (Figure 3A, lane 8). mcm6-cdc45-1 1 5 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 4 unspecified role unspecified role bait bait physical association physical association Fig 5D Interaction requires sld3.