IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Receptor-interacting protein 140 directly recruits histone deacetylases for gene silencing. Receptor-interacting protein 140 directly recruits histone deacetylases for gene silencing. J. Biol. Chem. (0021-9258) 2000 Wei LN., Hu X., Chandra D., Seto E., Farooqui M. Chromatin - Epigenetic interactions resulting in chromatin modulation weixx009@maroon.tc.umn.edu 27-Aug-2008: Contacted by JYOTI. davet in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Wei LN., Hu X., Chandra D., Seto E., Farooqui M. Chromatin - Epigenetic interactions resulting in chromatin modulation weixx009@maroon.tc.umn.edu Accepted 2008-SEP-11 by DAVET. 27-Aug-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2000 davet Receptor-interacting protein 140 directly recruits histone deacetylases for gene silencing. Receptor-interacting protein 140 directly recruits histone deacetylases for gene silencing. J. Biol. Chem. (0021-9258) 2000 Wei LN., Hu X., Chandra D., Seto E., Farooqui M. Chromatin - Epigenetic interactions resulting in chromatin modulation weixx009@maroon.tc.umn.edu 27-Aug-2008: Contacted by JYOTI. davet human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wei LN., Hu X., Chandra D., Seto E., Farooqui M. Chromatin - Epigenetic interactions resulting in chromatin modulation weixx009@maroon.tc.umn.edu Accepted 2008-SEP-11 by DAVET. J. Biol. Chem. (0021-9258) 2000 davet hdac3_human Histone deacetylase 3 HDAC3 RPD3-2 SMAP45 protein protein human Homo sapiens Human MAKTVAYFYDPDVGNFHYGAGHPMKPHRLALTHSLVLHYGLYKKMIVFKPYQASQHDMCRFHSEDYIDFLQRVSPTNMQGFTKSLNAFNVGDDCPVFPGLFEFCSRYTGASLQGATQLNNKICDIAINWAGGLHHAKKFEASGFCYVNDIVIGILELLKYHPRVLYIDIDIHHGDGVQEAFYLTDRVMTVSFHKYGNYFFPGTGDMYEVGAESGRYYCLNVPLRDGIDDQSYKHLFQPVINQVVDFYQPTCIVLQCGADSLGCDRLGCFNLSIRGHGECVEYVKSFNIPLLVLGGGGYTVRNVARCWTYETSLLVEEAISEELPYSEYFEYFAPDFTLHPDVSTRIENQNSRQYLDQIRQTIFENLKMLNHAPSVQIHDVPADLLTYDRTDEADAEERGPEENYSRPEAPNEFYDGDHDNDKESDVEI DIP protein O15379 original sequence version: 159 DIP protein O15379 original sequence version: 161 DIP protein O15379 original sequence version: 162 DIP protein O15379 original sequence version: 89 DIP protein O15379 original sequence version: 156 DIP protein O15379 original sequence version: 176 DIP protein O15379 original sequence version: 170 DIP protein O15379-1 original sequence version: 170 DIP protein O15379 original sequence version: 127 DIP protein O15379-1 original sequence version: 128 DIP protein O15379 original sequence version: 119 94485C1EBDCF5AD0 nrip1_mouse Nuclear receptor-interacting protein 1 Nrip1 Nuclear factor RIP140 Receptor-interacting protein 140 protein protein mouse Mus musculus Mouse MTHGEELGSDVHQDSIVLTYLEGLLMHQAAGGSGTAINKKSAGHKEEDQNFNLSGSAFPSCQSNGPTVSTQTYQGSGMLHLKKARLLQSSEDWNAAKRKRLSDSIVNLNVKKEALLAGMVDSVPKGKQDSTLLASLLQSFSSRLQTVALSQQIRQSLKEQGYALSHESLKVEKDLRCYGVASSHLKTLLKKSKTKDQKSGPTLPDVTPNLIRDSFVESSHPAVGQSGTKVMSEPLSCAARLQAVASMVEKRASPAASPKPSVACSQLALLLSSEAHLQQYSREHALKTQNAHQVASERLAAMARLQENGQKDVGSSQLSKGVSGHLNGQARALPASKLVANKNNAATFQSPMGVVPSSPKNTSYKNSLERNNLKQAANNSLLLHLLKSQTIPTPMNGHSQNERASSFESSTPTTIDEYSDNNPSFTDDSSGDESSYSNCVPIDLSCKHRIEKPEAERPVSLENLTQSLLNTWDPKIPGVDIKEDQDTSTNSKLNSHQKVTLLQLLLGHKSEETVERNASPQDIHSDGTKFSPQNYTRTSVIESPSTNRTTPVSTPPLYTASQAESPINLSQHSLVIKWNSPPYACSTPASKLTNTAPSHLMDLTKGKESQAEKPAPSEGAQNSATFSASKLLQNLAQCGLQSSGPGEEQRPCKQLLSGNPDKPLGLIDRLNSPLLSNKTNAAEESKAFSSQPAGPEPGLPGCEIENLLERRTVLQLLLGNSSKGKNEKKEKTPARDEAPQEHSERAANEQILMVKIKSEPCDDFQTHNTNLPLNHDAKSAPFLGVTPAIHRSTAALPVSEDFKSEPASPQDFSFSKNGLLSRLLRQNQESYPADEQDKSHRNSELPTLESKNICMVPKKRKLYTEPLENPFKKMKNTAVDTANHHSGPEVLYGSLLHQEELKFSRNELDYKYPAGHSSASDGDHRSWARESKSFNVLKQLLLSENCVRDLSPHRSDSVPDTKKKGHKNNAPGSKPEFGISSLNGLMYSSPQPGSCVTDHRTFSYPGMVKTPLSPPFPEHLGCVGSRPEPGLLNGCSVPGEKGPIKWVIADMDKNEYEKDSPRLTKTNPILYYMLQKGGGNSVTTQETQDKDIWREPASAESLSQVTVKEELLPAAETKASFFNLRSPYNSHMGNNASRPHSTNGEVYGLLGNALTIKKESE DIP protein Q8CBD1 original sequence version: 43 DIP protein Q8CBD1 original sequence version: 32 56ABD0C2A5AAC0A1 hdac1_human Histone deacetylase 1 HDAC1 RPD3L1 protein protein human Homo sapiens Human MAQTQGTRRKVCYYYDGDVGNYYYGQGHPMKPHRIRMTHNLLLNYGLYRKMEIYRPHKANAEEMTKYHSDDYIKFLRSIRPDNMSEYSKQMQRFNVGEDCPVFDGLFEFCQLSTGGSVASAVKLNKQQTDIAVNWAGGLHHAKKSEASGFCYVNDIVLAILELLKYHQRVLYIDIDIHHGDGVEEAFYTTDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNYPLRDGIDDESYEAIFKPVMSKVMEMFQPSAVVLQCGSDSLSGDRLGCFNLTIKGHAKCVEFVKSFNLPMLMLGGGGYTIRNVARCWTYETAVALDTEIPNELPYNDYFEYFGPDFKLHISPSNMTNQNTNEYLEKIKQRLFENLRMLPHAPGVQMQAIPEDAIPEESGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA DIP protein Q13547 original sequence version: 106 DIP protein Q13547 original sequence version: 173 DIP protein Q13547 original sequence version: 100 DIP protein Q13547 original sequence version: 166 DIP protein Q13547 original sequence version: 153 DIP protein Q13547 original sequence version: 164 DIP protein Q13547 original sequence version: 175 DIP protein Q13547 original sequence version: 191 DIP protein Q13547 original sequence version: 185 DIP protein Q13547 original sequence version: 165 DIP protein Q13547 original sequence version: 162 DIP protein Q13547 original sequence version: 189 DIP protein Q13547 original sequence version: 158 DIP protein Q13547 original sequence version: 134 DIP protein Q13547 original sequence version: 124 4D35B7C1ED7838D6 hdac3-nrip1 2 3 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey physical association physical association 2 A Q8CBD1; O15379 transfection co-immunoprecipitation of flag-HDAC and RIP140. nrip1-hdac3 2 3 unspecified role unspecified role bait bait 4 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2 B Q8CBD1; O15379 transfection where cells were co-transfected with HDAC3 plus an empty vector or a flag-RIP vector, the anti-HDAC3 antibody detected a band of approximately 52 kDa in the precipitate of the flag-RIP plus HDAC3- transfected culture nrip1-hdac1 1 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 4 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction 2 D The full-length RIP140 interacts with both HDAC 1 and 3