IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. Genes Dev. (0890-9369) 2000 Kal AJ., Mahmoudi T., Zak NB., Verrijzer CP. Chromatin - Epigenetic interactions resulting in chromatin modulation Drosophila embryo nuclear extract ammonium sulphate cut off 9-56 percent was further fractionated on POROS Heparin, S300, Q10 and monoS column. This compelx was used to study the interaction. verrijzer@lumc.nl orchard drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom western blot western blot Immuno blot Immuno blot Kal AJ., Mahmoudi T., Zak NB., Verrijzer CP. Chromatin - Epigenetic interactions resulting in chromatin modulation Drosophila embryo nuclear extract ammonium sulphate cut off 9-56 percent was further fractionated on POROS Heparin, S300, Q10 and monoS column. This compelx was used to study the interaction. verrijzer@lumc.nl Accepted 2007-JAN-11 by ORCHARD. Genes Dev. (0890-9369) 2000 orchard The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. Genes Dev. (0890-9369) 2000 Kal AJ., Mahmoudi T., Zak NB., Verrijzer CP. Chromatin - Epigenetic interactions resulting in chromatin modulation Drosophila embryo nuclear extract ammonium sulphate cut off 9-56 percent was further fractionated on POROS Heparin, S300, Q10 and monoS column. This compelx was used to study the interaction. verrijzer@lumc.nl orchard in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Kal AJ., Mahmoudi T., Zak NB., Verrijzer CP. Chromatin - Epigenetic interactions resulting in chromatin modulation Drosophila embryo nuclear extract ammonium sulphate cut off 9-56 percent was further fractionated on POROS Heparin, S300, Q10 and monoS column. This compelx was used to study the interaction. UniProtKB identifier for p400 could not be ascertained, hence the interaction between p400 and Zeste has not been entered. verrijzer@lumc.nl Accepted 2007-JAN-11 by ORCHARD. Genes Dev. (0890-9369) 2000 orchard The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. Genes Dev. (0890-9369) 2000 Kal AJ., Mahmoudi T., Zak NB., Verrijzer CP. Chromatin - Epigenetic interactions resulting in chromatin modulation Drosophila embryo nuclear extract ammonium sulphate cut off 9-56 percent was further fractionated on POROS Heparin, S300, Q10 and monoS column. This compelx was used to study the interaction. verrijzer@lumc.nl orchard drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kal AJ., Mahmoudi T., Zak NB., Verrijzer CP. Chromatin - Epigenetic interactions resulting in chromatin modulation Drosophila embryo nuclear extract ammonium sulphate cut off 9-56 percent was further fractionated on POROS Heparin, S300, Q10 and monoS column. This compelx was used to study the interaction. verrijzer@lumc.nl Accepted 2007-JAN-11 by ORCHARD. Genes Dev. (0890-9369) 2000 orchard The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. The Drosophila brahma complex is an essential coactivator for the trithorax group protein zeste. Genes Dev. (0890-9369) 2000 Kal AJ., Mahmoudi T., Zak NB., Verrijzer CP. Chromatin - Epigenetic interactions resulting in chromatin modulation Drosophila embryo nuclear extract ammonium sulphate cut off 9-56 percent was further fractionated on POROS Heparin, S300, Q10 and monoS column. This compelx was used to study the interaction. verrijzer@lumc.nl orchard in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kal AJ., Mahmoudi T., Zak NB., Verrijzer CP. Chromatin - Epigenetic interactions resulting in chromatin modulation Drosophila embryo nuclear extract ammonium sulphate cut off 9-56 percent was further fractionated on POROS Heparin, S300, Q10 and monoS column. This compelx was used to study the interaction. verrijzer@lumc.nl Accepted 2007-JAN-11 by ORCHARD. Genes Dev. (0890-9369) 2000 orchard q7kmj0_drome Bap170 BcDNA.GH12174 CG3274 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLLLSLTDVMMIIFTLEAVYALSALGARPCSMLMQVRGLIDQLVTLITVEAQSYGTGGCILMRVVEVFPGNMAASLAQNMPAILAPGPTSLTTLPTSLQVAVKPPAVLPTPTPAQTQVLPHGEQTPLVTALPGLPMPSLPQVQLPVPSLPQVQLPPIAATVTLPSLPSASQSFTHEDEQYALAWLGATYERAGNGNDLRVEQAELYRIYLSHCQKAGKLSVVNHMQFPRLVRLIFNQSVGPVIVRHLDGIELPGTYYVGIRMRAQPLAMQQRPTPSIVPVKKDTPILPKQPFSDPVQTEVASEESVSSAVTPPPSSSLIKSLLANKVTERQQKQKAQAQTPQPPALVATTASGTNATQPIKVTSTAISAFVNNPLMQHTPVKVGQTTIKPLHPQMAIEKKPITDSAPPPLAPLSGANVVTKDASGRTLIIASAAAKRKLTIDEEQNKRLALDAAASSSSSSSSGGKEEAQVTPSKNAANLYADLAASILEDEDMDDVPPLAKPSQEQSQQHPQQAQQLQLIPAKVQPTQRQLVFPGSNVVSPAPHSQLKLATTATIKTDQGLQTVPVILQPKTAMDSTPAPQTQYVLATNQQGQTYLVAQQPTIPTSTQSAPPTLLVTQTPQQQTKTIIILQQPGGVSAANVAGTQKMIMTTAQGQQVLVTTTTAPQLAQPRTSTPQQQIFIAPQRPVPALGAGQMSPSLLSQLNQIPATIKLHQPQPQPQLVSPQLAPQQRLVAAKPGASLPIPQLQQHQSIIQQHIISGPSTPTSTVGGTVGGEKRQVILGGIKETTLITQTSATPAPLQQSQLNSQTIITQSPTSTAIVGGVALQQQHHIRSVLDQQQQHHPSIIQQKITMPAISEATKPKIAPVPVSPTVTLLAKKPAPPQPAQVSSPKPSVTQAVVVTTSGTSDSTEGGKTVVLATSEAKPAESEVVAQTVVPPAVVTTGPSSSSLVVSQSVYAASGPNANPTPSPMPVDANWLYICDWRNCPRKKFKSLNELQYHVCSVHCPDHLDSDADIYCQWGSGPNFCDNRARKRYSLMTHLIDRHLTTENLRASVQRRLATGIHNVAPTQAPVTIVRNEGHAQRLAGGATGSPSGPASAVPVVGSAAMQAMNRHTTDYTNAKELMDENEGPVTKSIRLTAALIIRNLVTYSATAKRSLKRYESRLANVAFSNVEASGVLSHIMYELSQ 03C37328A91B455A q53yh4_drome bap CG7902 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLNMESAGVSAAMAGLSKSLTTPFSINDILTRSNPETRRMSSVDSEPEPEKLKPSSDRERSISKSPPLCCRDLGLYKLTQPKEIQPSARQPSNYLQYYAAAMDNNNHHHQATGTSNSSAADYMQRKLAYFGSTLAAPLDMRRCTSNDSDCDSPPPLSSSPSESPLSHDGSGLSRKKRSRAAFSHAQVFELERRFAQQRYLSGPERSEMAKSLRLTETQVKIWFQNRRYKTKRKQIQQHEAALLGASKRVPVQVLVREDGSTTYAHMAAPGAGHGLDPALINIYRHQLQLAYGGLPLPQMQMPFPYFYPQHKVPQPIPPPTQSSSFVTASSASSSPVPIPIPGAVRPQRTPCPSPNGQMMSVESGAESVHSAAEDVDENVEID 49A8DFE19A2022B9 osa_drome Trithorax group protein osa Protein eyelid CG7467 osa eld protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNEKIKSPQTQQQQQGGAPAPAATPPSAGAAPGAATPPTSGPPTPNNNSNNGSDPSIQQQQQNVAPHPYGAPPPPGSGPGGPPGPDPAAVMHYHHLHQQQQQHPPPPHMQQQQHHGGPAPPPPGGAPEHAPGVKEEYTHLPPPHPHPAYGRYHADPNMDPYRYGQPLPGGKPPQQQQPHPQQQPPQQPGPGGSPNRPPQQRYIPGQPPQGPTPTLNSLLQSSNPPPPPQHRYANTYDPQQAAASAAAAAAAQQQQAGGPPPPGHGPPPPQHQPSPYGGQQGGWAPPPRPYSPQLGPSQQYRTPPPTNTSRGQSPYPPAHGQNSGSYPSSPQQQQQQQQQQQQQAGQQPGGPVPGGPPPGTGQQPPQQNTPPTSQYSPYPQRYPTPPGLPAGGSNHRTAYSTHQYPEPNRPWPGGSSPSPGSGHPLPPASPHHVPPLQQQPPPPPHVSAGGPPPSSSPGHAPSPSPQPSQASPSPHQELIGQNSNDSSSGGAHSGMGSGPPGTPNPQQVMRPTPSPTGSSGSRSMSPAVAQNHPISRPASNQSSSGGPMQQPPVGAGGPPPMPPHPGMPGGPPQQQQSQQQQASNSASSASNSPQQTPPPAPPPNQGMNNMATPPPPPQGAAGGGYPMPPHMHGGYKMGGPGQSPGAQGYPPQQPQQYPPGNYPPRPQYPPGAYATGPPPPPTSQAGAGGANSMPSGAQAGGYPGRGMPNHTGQYPPYQWVPPSPQQTVPGGAPGGAMVGNHVQGKGTPPPPVVGGPPPPQGSGSPRPLNYLKQHLQHKGGYGGSPTPPQGPQGYGNGPTGMHPGMPMGPPHHMGPPHGPTNMGPPTSTPPQSQMLQGGQPQGQGASGGPESGGPEHISQDNGISSSGPTGAAGMHAVTSVVTTGPDGTSMDEVSQQSTLSNASAASGEDPQCTTPKSRKNDPYSQSHLAPPSTSPHPVVMHPGGGPGEEYDMSSPPNWPRPAGSPQVFNHVPVPQEPFRSTITTTKKSDSLCKLYEMDDNPDRRGWLDKLRAFMEERRTPITACPTISKQPLDLYRLYIYVKERGGFVEVTKSKTWKDIAGLLGIGASSSAAYTLRKHYTKNLLTFECHFDRGDIDPLPIIQQVEAGSKKKTAKAASVPSPGGGHLDAGTTNSTGSSNSQDSFPAPPGSAPNAAIDGYPGYPGGSPYPVASGPQPDYATAGQMQRPPSQNNPQTPHPGAAAAVAAGDNISVSNPFEDPIAAGGGPGSGTGPGPGQGPGPGAASGGAGAVGAVGGGPQPHPPPPHSPHTAAQQAAGQHQQQHPQHQHPGLPGPPPPQQQQGQQGQQPPPSVGGGPPPAPQQHGPGQVPPSPQQHVRPAAGAPYPPGGSGYPTPVSRTPGSPYPSQPGAYGQYGSSDQYNATGPPGQPFGQGPGQYPPQNRNMYPPYGPEGEAPPTGANQYGPYGSRPYSQPPPGGPQPPTQTVAGGPPAGGAPGAPPSSAYPTGRPSQQDYYQPPPDQSPQPRRHPDFIKDSQPYPGYNARPQIYGAWQSGTQQYRPQYPSSPAPQNWGGAPPRGAAPPPGAPHGPPIQQPAGVAQWDQHRYPPQQGPPPPPQQQQQPQQQQQQPPYQQVAGPPGQQPPQAPPQWAQMNPGQTAQSGIAPPGSPLRPPSGPGQQNRMPGMPAQQQQSQQQGGVPQPPPQQASHGGVPSPGLPQVGPGGMVKPPYAMPPPPSQGVGQQVGQGPPGGMMSQKPPPMPGQAMQQQPLQQQPPSHQHPHPHQHPQHQHPHQMPPNQTAPGGYGPPGMPGGGAQLVKKELIFPHDSVESTTPVLYRRKRLMKADVCPVDPWRIFMAMRSGLLTECTWALDVLNVLLFDDSTVQFFGISNLPGLLTLLLEHFQKNLAEMFDERENEEQSALLAEDADDDADSGTVMCEKLRTSGRQPRCVRSISSYNRRRHYENMDRSGKDGAGNGSDSEDADEGIDLGQVRVQPNPEERSLLLSFTPNYTMVTRKGVPVRIQPAENDIFVDERQKAWDIDTNRLYEQLEPVGSDAWTYGFTEPDPLDGIIDVFKSEIVNIPFARYIRSDKKGRKRTELASSSRKPEIKTEENSTEEQTFNKKRRLVSGGSSSSGAHAEGKKSKLTSEEFAQPNAEVKKEPGTADSDCRPVDMDIEAPQQRLTNGVAPCSSTPAIFDPRTTAKDEARVLQRRRDSSFEDECYTRDEASLHLVSESQDSLARRCIALSNIFRNLTFVPGNETVLAKSTRFLAVLGRLLLLNHEHLRRTPKTRNYDREEDTDFSDSCSSLQGEREWWWDYLITIRENMLVAMANIAGHLELSRYDELIARPLIDGLLHWAVCPSAHGQDPFPSCGPNSVLSPQRLALEALCKLCVTDANVDLVIATPPFSRLEKLCAVLTRHLCRNEDQVLREFSVNLLHYLAAADSAMARTVALQSPCISYLVAFIEQAEQTALGVANQHGINYLRENPDSMGTSLDMLRRAAGTLLHLAKHPDNRSLFMQQEQRLLGLVMSHILDQQVALIISRVLYQVSRGTGPIHSVEFRLLQQRQQQQLRPGPAGKQAASAGGSATVKAETASTETSSTEAKPAPAATTAVVNDENSNSSQQLPPAATFNDVSNSSTNSNSCGTASSNQTNNSTTNSSHSSSAISSQSAITVAAPSAAATGAGSATAAAIASDQQQVSKVAAAAAAAAALSNASAAAAAAAAAAAASVGPPTSSSVSAGAAVAQPAAPPPTNAGTTTAVA EFAE76CB51C7C675 brm_drome ATP-dependent helicase brm Homeotic gene regulator Protein brahma brm CG5942 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASPSPANSPMPPPQAPSPMAPPSQSPAPSPHSPYPHQQPGPLQGPPPPGHPGAYGHPMQHGPPGQGPPGHHMPPHHQGMIFSKGPHMGMQMPPTGPNMSPYQTHGMPPNAPTQPCIVSPGGPPGGPPPPERSSQENLHALQRAIDSMEEKGLQEDPRYSQLLAMRATSKHQHLNGNQVNLLRTQITAYRLLARNKPISMQMQQALQAAQQQPPPGPPIGPPGAPGGPPPGSQHAGQPPVPPQQQQQPPPSAGTPPQCSTPPASNPYGPPVPGQKMQVAPPPPHMQQGQPLPPQPPQVGGPPPIQQQQPPQQQQQQSQPPPPEPHQHQLPNGGKPLSMGPSGGQPLIPSSPMQPQVRGTLPGMPPGSQVPQPGGGPQRQVPPAGMPMPKPNRITTVAKPVGLDPITLLQERENRIAARISLRMQELQRLPATMSEDLRLQAAIELRALRVLNFQRQLRMEFVQCTRRDTTLETALNIKLYKRTKRQGLREARATEKLEKQQKLEAERKRRQKHLEFLAAVLQHGKDLREFHRNNKAQLARMNKAVMNHHANAEREQKKEQERIEKERMRRLMAEDEEGYRKLIDQKKDKRLAFLLSQTDEYISNLTQMVKQHKDDQMKKKEEEGKRLIQFKKELLMSGEYIGIDEGSIVADMRVHVVEQCTGKKLTGDDAPMLKHLHRWLNMHPGWDWIDDEEDSCGSNDDHKPKVEEQPTATEDATDKAQATGNDEDAKDLITKAKVEDDEYRTEEQTYYSIAHTIHEKVVEQASIMVNGTLKEYQIKGLEWLVSLYNNNLNGILADEMGLGKTIQTISLVTYLMDRKKVMGPYLIIVPLSTLPNWVLEFEKWAPAVGVVSYKGSPQGRRLLQNQMRATKFNVLLTTYEYVIKDKAVLAKIQWKYMIIDEGHRMKNHHCKLTQVLNTHYIAPYRLLLTGTPLQNKLPELWALLNFLLPSIFKSCSTFEQWFNAPFATTGEKVELNEEETILIIRRLHKVLRPFLLRRLKKEVEHQLPDKVEYIIKCDMSALQRVLYKHMQSKGVLLTDGSEKGKHGKGGAKALMNTIVQLRKLCNHPFMFQHIEEKYCDHTGGHGVVSGPDLYRVSGKFELLDRILPKLKATNHRVLLFCQMTQCMTIIEDYLGWRQFGYLRLDGTTKAEDRGELLRKFNAKGSDVFVFLLSTRAGGLGLNLQTADTVVIFDSDWNPHQDLQAQDRAHRIGQRNEVRVLRLMTVNSVEERILAAARYKLNMDEKVIQAGMFDQKSTGSERQQFLQTILHQDDNEEEEENEVPDDEMINMMIARSEEEIEIFKRMDAERKKEDEEIHPGRERLIDESELPDWLTKDDDEVERFHYQYDEDTILGRGSRQRKEVDYTDSLTEKEWLKAIDDGAEFDEEEEEDDSKRKRRKRKNRKEESDDDSLILKRRRRQNLDKRSKKQMHKIMSAVIKHNQDGRTLSEPFMKLPSRQRLPDYYEIIKRPVDIKKILQRIEDCKYADLNELEKDFMQLCQNAQIYNEEASLIYLDSIALQKVFVGARQRITAAADAAAVAAGDNTGEAHGNGGSDNSDNDDDDGGDDGSDDEEIATTSAAAVKMKLKLNKSLASAPATPTQSSSNVSSGAATTSKKQTRRKRSQKKYTISDDDDDDMD DIP protein P25439 original sequence version: 118 A4494B29F4F2E42A q7kpy3_drome mor CG18740 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MALSGGEKKTIYSQPYLLQYLAHSSEPITKESLAQLFIHFLQYVEAKLGKNSADPPATRIPMRCFLDFKSGGGLCIIFSTMFRFRAEQRGKKFDFSIGKNPTRKDPNIQLLIEIEQALVEADLYRIPYIYIRPEIEKGFEGKLREILDNRRVEIVSDEEDATHIVYPVVDPHPDEYARPIFKRGGHVMLHWYYFPESYDSWAVNNFDLPDHIPENPESPAERWRVSASWIVDLEQYNEWMAEEDYEVDEQGKKKTHKQRMSIDDIMSFGDEKKKPAASSGGGKQKRRRSPSPASSASTSKPGKRKRSPAVVHKKSRNDDDDEDLTRDLDDPPAEPNVQEVHKANAALQSTASPAPGGKSRGDNDMMPIKGGTMTDLDDEMTGGSAAQAMSTGDGENSQTGKTSDNSNTQEFSSSAKEDMEDNVTEQTHHIIVPSYSAWFDYNSIHVIEKRAMPEFFNSKNKSKTPEIYMAYRNFMIDTYRLNPTEYLTTTACRRNLAGDVCAIMRVHAFLEQWGLINYQIDADVRPTPMGPPPTSHFHILSDTPSGLQSINPQKTQQPSAAKTLLDLDKKPLGKDGGLELGDKSGLTGIKTEALENGAAGGLSSGVSQFGLKLDQYAKKPAAMRNRTAASMAREWTDQETLLLLEGLEMHKDDWNKVCEHVGSRTQDECILHFLRLPIEDPYLEDDGGFLGPLACQPIPFSKSGNPIMSTVAFLASVVDPRVAAAAAKAAMEEFAAIKDEVPATIMDNHLKNVEKASAGGKFNPNFGLANSGIAGTGNDKDDEEGKEGGASASAGGSDEEMKDLSKKDDDAKSKDNTKSDKTNTNTDSSSTSSSATGNTNNTDKKPKESSGSSPSGDKSAIKSDKSNKSSPTETAAAASGGEVDIKTEDSSGDGETKDGTEAKEGTGTGTGPLAVPKEGTFSENNMQTAAAAALASAAVKAKHLAALEERKIKSLVALLVETQMKKLEIKLRHFEELEATMEREREGLEYQRQQLITERQQFHLEQLKAAEFRARQQAHHRLQQELQGQAAAGSMILPQQQQLPQAQQPQQQQQTLPPHPHLAQQQQLPPHPHQLPPQSQPLAGPTAQHQPLPPHVVSSPNGAPFAAPPISLTGGLPPGAPTAIVTNPSDQTGPVAAGAAQSQAPTPMDTTPPSSGPVSDANAPPGSAMPPGGIPPANTAPIAGVAPSS 6E59C06E00B7C50A zest_drome Regulatory protein zeste z CG7803 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSAQGEGGGAGGSGGGGAGSDGGGNAGQSSTGSGTVAVTNGGNSSAKNQLPLTPRFTAEEKEVLYTLFHLHEEVIDIKHRKKQRNKYSVRETWDKIVKDFNSHPHVSAMRNIKQIQKFWLNSRLRKQYPYRDGSSSNLSSGSAKISSVSVSVASAVPQQQQQQHHQQHDSVKVEPEYQISPDASEHNPQADTFDEIEMDANDVSEIDEDPMEQQQQQQQEAQAQAQAQAQAQAQVQSAAAEMQKMQQVNAVAAAAAANATMINTHQINVDQISAEKLTLNDLLHFKTARPREEIILIKHPEATATQIHTIPTQAQQHPMATITAGGYNQQIISEIKPQQITLAQYQAQQQQQAQAQAQAQAQAQAQAQAQAQAQAQAQQLAQQQLAAAQHQQLAAAVQVHHQQQQQQQAAVAVQQQQAAAAAAVKMQLTAATPTFTFSALPTVTAATTVPAAVPVPVATASSGSANSVAVNTSTASSVSINNTSLGGGGGNGATNSSATAADSFEERMNYFKIREAELRCKEQQLATEAKRIELNKAQDELKYMKEVHRLRVEELTMKIRILQKEEEQLRKCSTS 65A8B77C93DF407A bap60_drome Brahma-associated protein of 60 kDa Bap60 BRM-associated protein 60 CG4303 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSQRFAPGQAPVQSRYQPPPPAPGMRPYPPPGASFPPRGFPLHPNTTQPVPGTANVAGVPGVPGVPGVPGPSLLQRAAQQPGFQSSLRGTGAGGGGVGSGGGSKRSNESRSLGGGGSKSDFATAKKKKKLAEKILPQKVRDLVPESQAYMDLLTFERKLDATIMRKRLDIQEALKRPMKQKRKLRIFISNTFYPSKEPTNDGEEGAVASWELRVEGRLLEDGKGDPNTKIKRKFSSFFKSLVIELDKELYGPDNHLVEWHRTHTTQETDGFQVKRPGDRNVRCTILLLLDYQPLQFKLDPRLARLLGVHTQTRPVIISALWQYIKTHKLQDAHEREYINCDKYLEQIFSCQRMKFAEIPQRLNPLLHPPDPIVINHFIESGAENKQTACYDIDVEVDDTLKNQMNSFLMSTASQQEIQGLDTKIHETVDTINQMKTNREFFLSFAKDPQMFIHRWIISETRDLKLMTDVAGNPEEERRAEFYYQPWTHEAVSRYFFTKVNQKRAELEQALGIRNG DIP protein Q9VYG2 original sequence version: 71 C7823B09A2DBDF2C q9biw6_drome Bap111 dalao CG7055 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MALPSNYKQIAVGGQGSATPLQGGGGGSGGGSRSRSSGGGGGGDRNKDQTPIFTHSNYGNPAFTPQKVTKSSSSKNQNESRLPKPPKPPEKPILPYMRYSKRVWDSVKAKHPELKLWELGKKIGAMWKLLPEDEKTEFIDEYEAEKLEYEKSLKAYHQTPAYQAYMSAKSKVKTDVDMHETPSRGGGSKSQHERRIDIQPAEDEDDQDEGYTTKHLAYARYLHNHRLINEIFSEAVVPDVRSVVTTTRMQVLKRQVSSLTMHQTKLEAELQQMEEKFEAKKQRMVESSEAFQEELKRHCKPAVDEETFQKMVLRMYEDIKRDRQRLDEPNANANSAANPAAAAATAAAAPVTRSEEAVKPPTQPGQPAATPAGQEPASAVPAPAAPPKETPPAVKPATLNPTPSSTPTPAPAVHVHETASKTDPEPMDIEPPPKPSVPPPPIKPEKLEMAAALPPQSTLVEPPKTEPAKVVAQPGKVPTPVPTPTPPPEVAPAAGAVTAATTTTRRTPTPPPVPQQVPLPQQQGGPAPPPGMPQMHPHPGHPPPGHSLMPPHMGPHQPPPGMPGLPPPPPHTGYANYGGPPHGPPPGPPGGPARPYYQPQYGGHPTPQPYYAPFSPYQQSYGPPPGSHYMSPRPPPPQHNGNPGHPYAPEHGSNPPPPQQQQQQQPPPGHLHEPSGGGPGAPGGGAGAAAAAAPGAGVYPTPGAGAGPGAPPGPAGGAPLGEAAVAGGVGPPAATALGKHPEAEKHEAD DIP protein Q9BIW6 original sequence version: 53 6717C5F3774D9790 brm-mor-1 3 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 3e The identity of the OSA protein in the core BRM complex was confirmed by Western blot analysis (Fig. 3E). brm-iswi 1 9 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component physical association physical association 3C The anti-OSA antibody efficiently recognized p300 as well as a number of bands migrating between 200 and 300 kD, which might be alternatively processed forms (Fig. 3C, lanes 1,2). The BRM and MOR subunits were readily detected by Western blot analysis (Fig. 3C, lanes 3-6), osa-zeste 2 7 unspecified role unspecified role prey prey drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. 10 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 6B Following denaturation and renaturation, the membrane was probed with radiolabeled Zeste (Fig. 6B). Autoradiography of the blot revealed that Zeste directly bound to a number of BAPs, most notably MOR, BAP170, and p400. Somewhat less prominent binding was observed to BAP111 and OSA. mor-zeste-1 2 10 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 9 unspecified role unspecified role prey prey drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. direct interaction direct interaction 6B Following denaturation and renaturation, the membrane was probed with radiolabeled Zeste (Fig. 6B). Autoradiography of the blot revealed that Zeste directly bound to a number of BAPs, most notably MOR, BAP170, and p400. Somewhat less prominent binding was observed to BAP111 and OSA. mor-brm-1 4 8 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 6C The domain structure of MOR and its interaction domains with itself, BRM mor-mor-1 4 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 6C The domain structure of MOR and its interaction domains with itself, BRM The regions shown in the features are as described by author. The UniProtKB entry has 1189 AA while the entry shown by the authors has 1174 AA. mor-zeste 4 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain in vitro In vitro 43 46 direct interaction direct interaction 6C, 7 We were able to further narrow the Zeste binding domain of MOR to amino acids 171 to 410 (Fig. 6C, lanes 6-8). The regions shown in the features are as described by author. The UniProtKB entry has 1189 AA while the entry shown by the authors has 1174 AA. We found that the leucine-zipper domain (residues 501-575) of Zeste was necessary and sufficient for binding to MOR (Fig. 7). bap111-zeste 2 12 unspecified role unspecified role prey prey drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. 10 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 6B Following denaturation and renaturation, the membrane was probed with radiolabeled Zeste (Fig. 6B). Autoradiography of the blot revealed that Zeste directly bound to a number of BAPs, most notably MOR, BAP170, and p400. Somewhat less prominent binding was observed to BAP111 and OSA. bap170-zeste 2 5 unspecified role unspecified role prey prey drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. 10 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction 6B Following denaturation and renaturation, the membrane was probed with radiolabeled Zeste (Fig. 6B). Autoradiography of the blot revealed that Zeste directly bound to a number of BAPs, most notably MOR, BAP170, and p400. Somewhat less prominent binding was observed to BAP111 and OSA. mor-brm 2 9 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role prey prey drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. direct interaction direct interaction 6B When radiolabeled MOR was used as a probe in a far-Western assay, the pattern of binding was very different (Fig. 6B, lane 4). MOR bound to BRM and itself, as was previously reported (Crosby et al. 1999), as well as to BAP111, BAP60 and to either BAP47 or BAP45. mor-bap111 2 9 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 12 unspecified role unspecified role prey prey drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. direct interaction direct interaction 6B When radiolabeled MOR was used as a probe in a far-Western assay, the pattern of binding was very different (Fig. 6B, lane 4). MOR bound to BRM and itself, as was previously reported (Crosby et al. 1999), as well as to BAP111, BAP60 and to either BAP47 or BAP45. mor-bap60 2 9 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 11 unspecified role unspecified role prey prey drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. direct interaction direct interaction 6B When radiolabeled MOR was used as a probe in a far-Western assay, the pattern of binding was very different (Fig. 6B, lane 4). MOR bound to BRM and itself, as was previously reported (Crosby et al. 1999), as well as to BAP111, BAP60 and to either BAP47 or BAP45. mor-bap45 2 9 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 6 unspecified role unspecified role prey prey drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. direct interaction direct interaction 6B When radiolabeled MOR was used as a probe in a far-Western assay, the pattern of binding was very different (Fig. 6B, lane 4). MOR bound to BRM and itself, as was previously reported (Crosby et al. 1999), as well as to BAP111, BAP60 and to either BAP47 or BAP45. mor-mor-2 2 9 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 9 unspecified role unspecified role prey prey drome-embryo drosophilla melanogaster embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. direct interaction direct interaction 6B When radiolabeled MOR was used as a probe in a far-Western assay, the pattern of binding was very different (Fig. 6B, lane 4). MOR bound to BRM and itself, as was previously reported (Crosby et al. 1999), as well as to BAP111, BAP60 and to either BAP47 or BAP45.