bhf-ucl ApoE3 binding to tau tandem repeat I is abolished by tau serine262 phosphorylation. ApoE3 binding to tau tandem repeat I is abolished by tau serine262 phosphorylation. Neurosci. Lett. (0304-3940) 1995 imex curation Huang D.Y., Weisgraber K.H., Goedert M., Saunders A.M., Roses A.D., Strittmatter W.J. Cardiac - Interactions involving cardiac related proteins Human tau peptides were incubated with either ApoE3 or ApoE4. After the incubation, proteins were submitted to electrophoresis and transferred to a membrane that was incubated with the anti-tau serum 135 primary antibody. Apo E purified from human plasma confirmed in reference 19: Rall S.C. et al 1986 (PMID: 3724506) warren@neuro.duke.edu 02-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro electrophoresis electrophoretic mobility-based method electrophoresis western blot western blot Immuno blot Immuno blot Huang D.Y., Weisgraber K.H., Goedert M., Saunders A.M., Roses A.D., Strittmatter W.J. Cardiac - Interactions involving cardiac related proteins Human tau peptides were incubated with either ApoE3 or ApoE4. After the incubation, proteins were submitted to electrophoresis and transferred to a membrane that was incubated with the anti-tau serum 135 primary antibody. Apo E purified from human plasma confirmed in reference 19: Rall S.C. et al 1986 (PMID: 3724506) warren@neuro.duke.edu Accepted 2014-MAR-19 by MGT Neurosci. Lett. (0304-3940) 1995 Only protein-protein interactions imex curation tau_human Microtubule-associated protein tau MAPT MTBT1 TAU Neurofibrillary tangle protein Paired helical filament-tau MAPTL protein protein human Homo sapiens Human MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLEDEAAGHVTQEPESGKVVQEGFLREPGPPGLSHQLMSGMPGAPLLPEGPREATRQPSGTGPEDTEGGRHAPELLKHQLLGDLHQEGPPLKGAGGKERPGSKEEVDEDRDVDESSPQDSPPSKASPAQDGRPPQTAAREATSIPGFPAEGAIPLPVDFLSKVSTEIPASEPDGPSVGRAKGQDAPLEFTFHVEITPNVQKEQAHSEEHLGRAAFPGAPGEGPEARGPSLGEDTKEADLPEPSEKQPAAAPRGKPVSRVPQLKARMVSKSKDGTGSDDKKAKTSTRSSAKTLKNRPCLSPKHPTPGSSDPLIQPSSPAVCPEPPSSPKYVSSVTSRTGSSGAKEMKLKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGSKDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL DIP protein P10636 original sequence version: 125 DIP protein P10636 original sequence version: 210 D46C66CDBCD196E8 p02649-pro_0000001987 Apolipoprotein E APOE protein protein human Homo sapiens Human KVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH 19 317 E765BECDD0C05A55 apoe-mapt-1 1 3 unspecified role unspecified role neutral component neutral component human-plasma homo sapiens blood plasma plasma blood plasma 2 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P10636:p.Cys639Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain C A Wed May 02 15:06:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys639ala' Tue Jun 30 19:06:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys639Ala' phosphoserine 579 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain Phosphorylation of Serine 579 of Tau protein peptide I completely disrupts its interaction with ApoE3 chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig 1, 2, 3 apoe-mapt-2 1 2 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role neutral component neutral component cys130arg variant variant certain certain sequence position certain certain certain sequence position certain human-plasma homo sapiens blood plasma plasma blood plasma direct interaction direct interaction Fig 1 apoe-apoe-4 1 2 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P10636:p.Cys639Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain C A Wed May 02 14:43:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys639ala' Tue Jun 30 19:03:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys639Ala' chemical synthesis Chemical synthesis (Chemical synthesis) 3 unspecified role unspecified role neutral component neutral component human-plasma homo sapiens blood plasma plasma blood plasma 3 unspecified role unspecified role neutral component neutral component human-plasma homo sapiens blood plasma plasma blood plasma physical association physical association Fig 2