IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Bidirectional cross-regulation between the endothelial nitric oxide synthase and β-catenin signalling pathways. Bidirectional cross-regulation between the endothelial nitric oxide synthase and β-catenin signalling pathways. Cardiovascular research 2014 imex curation Warboys CM., Chen N., Zhang Q., Shaifta Y., Vanderslott G., Passacquale G., Hu Y., Xu Q., Ward JP., Ferro A. Cardiac - Interactions involving cardiac related proteins Affinomics - Interactions curated for the Affinomics consortium. albert.ferro@kcl.ac.uk Only protein-protein interactions imex curation human-endothelium Homo sapiens endothelail cells/endothelium endothelium Endothelial cells [endothelium] pla proximity ligation assay pELISA pELISA PLA antibody detection identification by antibody antibody detection Warboys CM., Chen N., Zhang Q., Shaifta Y., Vanderslott G., Passacquale G., Hu Y., Xu Q., Ward JP., Ferro A. Cardiac - Interactions involving cardiac related proteins HUVECs:Human umbilical vein endothelial cells albert.ferro@kcl.ac.uk Accepted 2015-APR-07 by ORCHARD Cardiovascular research 2014 Only protein-protein interactions imex curation imex curation Bidirectional cross-regulation between the endothelial nitric oxide synthase and β-catenin signalling pathways. Bidirectional cross-regulation between the endothelial nitric oxide synthase and β-catenin signalling pathways. Cardiovascular research 2014 imex curation Warboys CM., Chen N., Zhang Q., Shaifta Y., Vanderslott G., Passacquale G., Hu Y., Xu Q., Ward JP., Ferro A. Cardiac - Interactions involving cardiac related proteins Affinomics - Interactions curated for the Affinomics consortium. albert.ferro@kcl.ac.uk Only protein-protein interactions imex curation human-endothelium Homo sapiens endothelail cells/endothelium endothelium Endothelial cells [endothelium] anti bait coip anti bait coimmunoprecipitation anti bait coip fingerprinting peptide massfingerprinting fingerprinting Warboys CM., Chen N., Zhang Q., Shaifta Y., Vanderslott G., Passacquale G., Hu Y., Xu Q., Ward JP., Ferro A. Cardiac - Interactions involving cardiac related proteins HUVEC lysates were immunoprecipitated with eNOS antibody and immunoprecipitates were separated by SDS-PAGE. It is stated that Coomassie stained bands were excised for peptide mass fingerprinting. (however Supplementary fig 1 clearly shows MS/MS fragmentation results.) HUVECs:Human umbilical vein endothelial cells albert.ferro@kcl.ac.uk Accepted 2015-APR-07 by ORCHARD Cardiovascular research 2014 Only protein-protein interactions imex curation imex curation Bidirectional cross-regulation between the endothelial nitric oxide synthase and β-catenin signalling pathways. Bidirectional cross-regulation between the endothelial nitric oxide synthase and β-catenin signalling pathways. Cardiovascular research 2014 imex curation Warboys CM., Chen N., Zhang Q., Shaifta Y., Vanderslott G., Passacquale G., Hu Y., Xu Q., Ward JP., Ferro A. Cardiac - Interactions involving cardiac related proteins Affinomics - Interactions curated for the Affinomics consortium. albert.ferro@kcl.ac.uk Only protein-protein interactions imex curation human-endothelium Homo sapiens endothelail cells/endothelium endothelium Endothelial cells [endothelium] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Warboys CM., Chen N., Zhang Q., Shaifta Y., Vanderslott G., Passacquale G., Hu Y., Xu Q., Ward JP., Ferro A. Cardiac - Interactions involving cardiac related proteins HUVECs:Human umbilical vein endothelial cells albert.ferro@kcl.ac.uk Accepted 2015-APR-07 by ORCHARD Cardiovascular research 2014 Only protein-protein interactions imex curation imex curation nos3_human Nitric oxide synthase, endothelial Endothelial NOS EC-NOS NOS type III Constitutive NOS NOS3 protein protein human Homo sapiens Human MGNLKSVAQEPGPPCGLGLGLGLGLCGKQGPATPAPEPSRAPASLLPPAPEHSPPSSPLTQPPEGPKFPRVKNWEVGSITYDTLSAQAQQDGPCTPRRCLGSLVFPRKLQGRPSPGPPAPEQLLSQARDFINQYYSSIKRSGSQAHEQRLQEVEAEVAATGTYQLRESELVFGAKQAWRNAPRCVGRIQWGKLQVFDARDCRSAQEMFTYICNHIKYATNRGNLRSAITVFPQRCPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQHGWTPGNGRFDVLPLLLQAPDDPPELFLLPPELVLEVPLEHPTLEWFAALGLRWYALPAVSNMLLEIGGLEFPAAPFSGWYMSTEIGTRNLCDPHRYNILEDVAVCMDLDTRTTSSLWKDKAAVEINVAVLHSYQLAKVTIVDHHAATASFMKHLENEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYFLSPAFRYQPDPWKGSAAKGTGITRKKTFKEVANAVKISASLMGTVMAKRVKATILYGSETGRAQSYAQQLGRLFRKAFDPRVLCMDEYDVVSLEHETLVLVVTSTFGNGDPPENGESFAAALMEMSGPYNSSPRPEQHKSYKIRFNSISCSDPLVSSWRRKRKESSNTDSAGALGTLRFCVFGLGSRAYPHFCAFARAVDTRLEELGGERLLQLGQGDELCGQEEAFRGWAQAAFQAACETFCVGEDAKAAARDIFSPKRSWKRQRYRLSAQAEGLQLLPGLIHVHRRKMFQATIRSVENLQSSKSTRATILVRLDTGGQEGLQYQPGDHIGVCPPNRPGLVEALLSRVEDPPAPTEPVAVEQLEKGSPGGPPPGWVRDPRLPPCTLRQALTFFLDITSPPSPQLLRLLSTLAEEPREQQELEALSQDPRRYEEWKWFRCPTLLEVLEQFPSVALPAPLLLTQLPLLQPRYYSVSSAPSTHPGEIHLTVAVLAYRTQDGLGPLHYGVCSTWLSQLKPGDPVPCFIRGAPSFRLPPDPSLPCILVGPGTGIAPFRGFWQERLHDIESKGLQPTPMTLVFGCRCSQLDHLYRDEVQNAQQRGVFGRVLTAFSREPDNPKTYVQDILRTELAAEVHRVLCLERGHMFVCGDVTMATNVLQTVQRILATEGDMELDEAGDVIGVLRDQQRYHEDIFGLTLRTQEVTSRIRTQSFSLQERQLRGAVPWAFDPPGSDTNSP DIP protein P29474 original sequence version: 179 DIP protein P29474 original sequence version: 175 DIP protein P29474 original sequence version: 112 DIP protein P29474 original sequence version: 140 DIP protein P29474 original sequence version: 158 B761A6D40B1A5649 ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein P35222 original sequence version: 187 DIP protein P35222 original sequence version: 188 DIP protein P35222 original sequence version: 180 DIP protein P35222-1 original sequence version: 181 DIP protein P35222 original sequence version: 181 DIP protein P35222 original sequence version: 107 DIP protein P35222-1 original sequence version: 107 DIP protein P35222 original sequence version: 103 DIP protein P35222-1 original sequence version: 103 DIP protein P35222 original sequence version: 175 DIP protein P35222 original sequence version: 136 DIP protein P35222 original sequence version: 135 DIP protein P35222 original sequence version: 131 DIP protein P35222-1 original sequence version: 133 DIP protein P35222 original sequence version: 133 DIP protein P35222 original sequence version: 148 DIP protein P35222-1 original sequence version: 139 DIP protein P35222 original sequence version: 139 DIP protein P35222 original sequence version: 146 DIP protein P35222 original sequence version: 196 DIP protein P35222 original sequence version: 192 DIP protein P35222 original sequence version: 185 DIP protein P35222 original sequence version: 151 DIP protein P35222-1 original sequence version: 151 DIP protein P35222-1 original sequence version: 171 DIP protein P35222-1 original sequence version: 148 DIP protein P35222 original sequence version: 177 DIP protein P35222 original sequence version: 178 DIP protein P35222-1 original sequence version: 178 CB78F165A3EEF86E 314eqe-1 1 4 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component proximity proximity close-contact colocalization neighbourhood interaction 1B, 2C Amplification products (red blobs) were observed throughout the cells, particularly at cell borders. adenosine salbutamol- a short-acting beta-2-adrenergic receptor agonist histamine thrombin ctnnb1-nos3-1 3 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 1A Amplification products (red blobs) were observed throughout the cells, particularly at cell borders. nos3-ctnnb1-1 3 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 1A Amplification products (red blobs) were observed throughout the cells, particularly at cell borders. nos3-ctnnb1-2 2 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association Supp Fig 1 Amplification products (red blobs) were observed throughout the cells, particularly at cell borders.