bhf-ucl The major chemical-detoxifying system of UDP-glucuronosyltransferases requires regulated phosphorylation supported by protein kinase C. The major chemical-detoxifying system of UDP-glucuronosyltransferases requires regulated phosphorylation supported by protein kinase C. J. Biol. Chem. (0021-9258) 2008 imex curation Basu N.K., Kole L., Basu M., Chakraborty K., Mitra P.S., Owens I.S. Cardiac - Interactions involving cardiac related proteins UGT1A7 and UGT1A10 were human genes. Confirmed in Basu N. K. et al. 2004 (PMID: 14557274) owensi@mail.nih.gov 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Basu N.K., Kole L., Basu M., Chakraborty K., Mitra P.S., Owens I.S. Cardiac - Interactions involving cardiac related proteins UGT1A7 and UGT1A10 were human genes. Confirmed in Basu N. K. et al. 2004 (PMID: 14557274) owensi@mail.nih.gov Accepted 2014-JUL-03 by ORCHARD J. Biol. Chem. (0021-9258) 2008 imex curation The major chemical-detoxifying system of UDP-glucuronosyltransferases requires regulated phosphorylation supported by protein kinase C. The major chemical-detoxifying system of UDP-glucuronosyltransferases requires regulated phosphorylation supported by protein kinase C. J. Biol. Chem. (0021-9258) 2008 imex curation Basu N.K., Kole L., Basu M., Chakraborty K., Mitra P.S., Owens I.S. Cardiac - Interactions involving cardiac related proteins UGT1A7 and UGT1A10 were human genes. Confirmed in Basu N. K. et al. 2004 (PMID: 14557274) owensi@mail.nih.gov 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Basu N.K., Kole L., Basu M., Chakraborty K., Mitra P.S., Owens I.S. Cardiac - Interactions involving cardiac related proteins UGT1A7 and UGT1A10 were human genes. Confirmed in Basu N. K. et al. 2004 (PMID: 14557274) owensi@mail.nih.gov Accepted 2014-JUL-03 by ORCHARD J. Biol. Chem. (0021-9258) 2008 imex curation The major chemical-detoxifying system of UDP-glucuronosyltransferases requires regulated phosphorylation supported by protein kinase C. The major chemical-detoxifying system of UDP-glucuronosyltransferases requires regulated phosphorylation supported by protein kinase C. J. Biol. Chem. (0021-9258) 2008 imex curation Basu N.K., Kole L., Basu M., Chakraborty K., Mitra P.S., Owens I.S. Cardiac - Interactions involving cardiac related proteins UGT1A7 and UGT1A10 were human genes. Confirmed in Basu N. K. et al. 2004 (PMID: 14557274) owensi@mail.nih.gov 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Basu N.K., Kole L., Basu M., Chakraborty K., Mitra P.S., Owens I.S. Cardiac - Interactions involving cardiac related proteins UGT1A7 and UGT1A10 were human genes. Confirmed in Basu N. K. et al. 2004 (PMID: 14557274) owensi@mail.nih.gov Accepted 2014-JUL-03 by ORCHARD J. Biol. Chem. (0021-9258) 2008 imex curation ud17_human UDP-glucuronosyltransferase 1A7 UDP-glucuronosyltransferase 1-7 UGT1A7 GNT1 UGT1 UDP-glucuronosyltransferase 1-G protein protein human Homo sapiens Human MARAGWTGLLPLYVCLLLTCGFAKAGKLLVVPMDGSHWFTMQSVVEKLILRGHEVVVVMPEVSWQLGRSLNCTVKTYSTSYTLEDQDREFMVFADARWTAPLRSAFSLLTSSSNGIFDLFFSNCRSLFNDRKLVEYLKESCFDAVFLDPFDACGLIVAKYFSLPSVVFARGIFCHYLEEGAQCPAPLSYVPRLLLGFSDAMTFKERVWNHIMHLEEHLFCPYFFKNVLEIASEILQTPVTAYDLYSHTSIWLLRTDFVLEYPKPVMPNMIFIGGINCHQGKPVPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH 7D759A382AE871D3 pkcalpha_chlae_protein PKalpha chlae protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to UniProt P17252 pkcepsilon_chlae_protein PKCepsilon chlae protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to UniProt Q02156 kpce_human Protein kinase C epsilon type nPKC-epsilon PRKCE PKCE protein protein human Homo sapiens Human MVVFNGLLKIKICEAVSLKPTAWSLRHAVGPRPQTFLLDPYIALNVDDSRIGQTATKQKTNSPAWHDEFVTDVCNGRKIELAVFHDAPIGYDDFVANCTIQFEELLQNGSRHFEDWIDLEPEGRVYVIIDLSGSSGEAPKDNEERVFRERMRPRKRQGAVRRRVHQVNGHKFMATYLRQPTYCSHCRDFIWGVIGKQGYQCQVCTCVVHKRCHELIITKCAGLKKQETPDQVGSQRFSVNMPHKFGIHNYKVPTFCDHCGSLLWGLLRQGLQCKVCKMNVHRRCETNVAPNCGVDARGIAKVLADLGVTPDKITNSGQRRKKLIAGAESPQPASGSSPSEEDRSKSAPTSPCDQEIKELENNIRKALSFDNRGEEHRAASSPDGQLMSPGENGEVRQGQAKRLGLDEFNFIKVLGKGSFGKVMLAELKGKDEVYAVKVLKKDVILQDDDVDCTMTEKRILALARKHPYLTQLYCCFQTKDRLFFVMEYVNGGDLMFQIQRSRKFDEPRSRFYAAEVTSALMFLHQHGVIYRDLKLDNILLDAEGHCKLADFGMCKEGILNGVTTTTFCGTPDYIAPEILQELEYGPSVDWWALGVLMYEMMAGQPPFEADNEDDLFESILHDDVLYPVWLSKEAVSILKAFMTKNPHKRLGCVASQNGEDAIKQHPFFKEIDWVLLEQKKIKPPFKPRIKTKRDVNNFDQDFTREEPVLTLVDEAIVKQINQEEFKGFSYFGEDLMP DIP protein Q02156 original sequence version: 154 DIP protein Q02156 original sequence version: 119 85032D0A091A1F7F epsilon-rack_chlae_protein epsilon-RACK chale protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to UniProt P63244 ud110_human UDP-glucuronosyltransferase 1A10 UDP-glucuronosyltransferase 1-10 UGT1A10 GNT1 UGT1 UDP-glucuronosyltransferase 1-J protein protein human Homo sapiens Human MARAGWTSPVPLCVCLLLTCGFAEAGKLLVVPMDGSHWFTMQSVVEKLILRGHEVVVVMPEVSWQLERSLNCTVKTYSTSYTLEDQNREFMVFAHAQWKAQAQSIFSLLMSSSSGFLDLFFSHCRSLFNDRKLVEYLKESSFDAVFLDPFDTCGLIVAKYFSLPSVVFTRGIFCHHLEEGAQCPAPLSYVPNDLLGFSDAMTFKERVWNHIVHLEDHLFCQYLFRNALEIASEILQTPVTAYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH 73774EEEC7FE37BA pkcdelta_chlae_protein PKCdelta chlae protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey similar to UniProt Q05655 ugt1a7-epsilon_rack-1 3 4 unspecified role unspecified role bait bait c-terminus his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey physical association physical association Fig. 5B Curcumin Calphostin ugt1a10-pkcalpha-1 3 9 unspecified role unspecified role bait bait c-terminal his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey physical association physical association Fig. 5C Curcumin inhibits binding of UGT1A10 to PKC delta Calphostin inhibits binding of UGT1A10 to PKC delta pkcalpha-ugt1a10-1 2 9 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4C pkcdelta-ugt1a10-1 2 9 unspecified role unspecified role neutral component neutral component c-terminus his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4D pkcepsilon-ugt1a7-1 2 4 unspecified role unspecified role neutral component neutral component c-terminus his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3SA prkce-ugt1a7 1 4 enzyme target enzyme target substrate substrate unspecified role unspecified role c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 7 enzyme enzyme unspecified role unspecified role phosphorylation phosphorylation reaction phosphorylation Fig. 6E Q02156 increases activity of Q9HAW7 Q9HAW7 phosphorylated residue