bhf-ucl A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene. A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene. J. Biol. Chem. (0021-9258) 2006 imex curation Song K.H., Li T., Chiang J.Y. Cardiac - Interactions involving cardiac related proteins The author has confirmed that Prox1, SHP, SF-1, HNF4-alpha, FTF and PGC1-alpha were all of human origin. jchiang@neoucom.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hep_g2 Homo sapiens hepatocellular carcinoma (HCC) cells (p53-wt). hep_g2 human hepatocellular carcinoma (HCC) cells (p53-wt). ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Song K.H., Li T., Chiang J.Y. Cardiac - Interactions involving cardiac related proteins The author has confirmed that Prox1, SHP, SF-1, HNF4-alpha, FTF and PGC1-alpha were all of human origin. jchiang@neoucom.edu Accepted 2014-NOV-06 by MGT J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene. A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene. J. Biol. Chem. (0021-9258) 2006 imex curation Song K.H., Li T., Chiang J.Y. Cardiac - Interactions involving cardiac related proteins The author has confirmed that Prox1, SHP, SF-1, HNF4-alpha, FTF and PGC1-alpha were all of human origin. jchiang@neoucom.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Song K.H., Li T., Chiang J.Y. Cardiac - Interactions involving cardiac related proteins The author has confirmed that Prox1, SHP, SF-1, HNF4-alpha, FTF and PGC1-alpha were all of human origin. jchiang@neoucom.edu Accepted 2014-NOV-06 by MGT J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene. A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene. J. Biol. Chem. (0021-9258) 2006 imex curation Song K.H., Li T., Chiang J.Y. Cardiac - Interactions involving cardiac related proteins The author has confirmed that Prox1, SHP, SF-1, HNF4-alpha, FTF and PGC1-alpha were all of human origin. jchiang@neoucom.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast lexa b52 complement lexa b52 complementation LexA B52 transcription complementation lexa b52 complement predetermined predetermined participant predetermined Song K.H., Li T., Chiang J.Y. Cardiac - Interactions involving cardiac related proteins The author has confirmed that Prox1, SHP, SF-1, HNF4-alpha, FTF and PGC1-alpha were all of human origin. jchiang@neoucom.edu Accepted 2014-NOV-06 by MGT J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene. A Prospero-related homeodomain protein is a novel co-regulator of hepatocyte nuclear factor 4alpha that regulates the cholesterol 7alpha-hydroxylase gene. J. Biol. Chem. (0021-9258) 2006 imex curation Song K.H., Li T., Chiang J.Y. Cardiac - Interactions involving cardiac related proteins The author has confirmed that Prox1, SHP, SF-1, HNF4-alpha, FTF and PGC1-alpha were all of human origin. jchiang@neoucom.edu 12-Nov-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hepatocytes Human-hepatocytes primary hepatocytes primary hepatocytes anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Song K.H., Li T., Chiang J.Y. Cardiac - Interactions involving cardiac related proteins The author has confirmed that Prox1, SHP, SF-1, HNF4-alpha, FTF and PGC1-alpha were all of human origin. jchiang@neoucom.edu Accepted 2014-NOV-06 by MGT J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation prgc1_human Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Ligand effect modulator 6 PPARGC1A LEM6 PGC1 PGC1A PPARGC1 protein protein human Homo sapiens Human MAWDMCNQDSESVWSDIECAALVGEDQPLCPDLPELDLSELDVNDLDTDSFLGGLKWCSDQSEIISNQYNNEPSNIFEKIDEENEANLLAVLTETLDSLPVDEDGLPSFDALTDGDVTTDNEASPSSMPDGTPPPQEAEEPSLLKKLLLAPANTQLSYNECSGLSTQNHANHNHRIRTNPAIVKTENSWSNKAKSICQQQKPQRRPCSELLKYLTTNDDPPHTKPTENRNSSRDKCTSKKKSHTQSQSQHLQAKPTTLSLPLTPESPNDPKGSPFENKTIERTLSVELSGTAGLTPPTTPPHKANQDNPFRASPKLKSSCKTVVPPPSKKPRYSESSGTQGNNSTKKGPEQSELYAQLSKSSVLTGGHEERKTKRPSLRLFGDHDYCQSINSKTEILINISQELQDSRQLENKDVSSDWQGQICSSTDSDQCYLRETLEASKQVSPCSTRKQLQDQEIRAELNKHFGHPSQAVFDDEADKTGELRDSDFSNEQFSKLPMFINSGLAMDGLFDDSEDESDKLSYPWDGTQSYSLFNVSPSCSSFNSPCRDSVSPPKSLFSQRPQRMRSRSRSFSRHRSCSRSPYSRSRSRSPGSRSSSRSCYYYESSHYRHRTHRNSPLYVRSRSRSPYSRRPRYDSYEEYQHERLKREEYRREYEKRESERAKQRERQRQKAIEERRVIYVGKIRPDTTRTELRDRFEVFGEIEECTVNLRDDGDSYGFITYRYTCDAFAALENGYTLRRSNETDFELYFCGRKQFFKSNYADLDSNSDDFDPASTKSKYDSLDFDSLLKEAQRSLRR DIP protein Q9UBK2 original sequence version: 127 DIP protein Q9UBK2 original sequence version: 104 97DDD3643051A5F1 cp7a1_human_gene Cholesterol 7-alpha-monooxygenase CYP7A1 CYP7 CYPVII Cholesterol 7-alpha-hydroxylase Cytochrome P450 7A1 gene gene human Homo sapiens Human stf1_human Steroidogenic factor 1 Nuclear receptor subfamily 5 group A member 1 Adrenal 4-binding protein Steroid hormone receptor Ad4BP Fushi tarazu factor homolog 1 NR5A1 AD4BP FTZF1 SF1 protein protein human Homo sapiens Human MDYSYDEDLDELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKHYTCTESQSCKIDKTQRKRCPFCRFQKCLTVGMRLEAVRADRMRGGRNKFGPMYKRDRALKQQKKAQIRANGFKLETGPPMGVPPPPPPAPDYVLPPSLHGPEPKGLAAGPPAGPLGDFGAPALPMAVPGAHGPLAGYLYPAFPGRAIKSEYPEPYASPPQPGLPYGYPEPFSGGPNVPELILQLLQLEPDEDQVRARILGCLQEPTKSRPDQPAAFGLLCRMADQTFISIVDWARRCMVFKELEVADQMTLLQNCWSELLVFDHIYRQVQHGKEGSILLVTGQEVELTTVATQAGSLLHSLVLRAQELVLQLLALQLDRQEFVCLKFIILFSLDLKFLNNHILVKDAQEKANAALLDYTLCHYPHCGDKFQQLLLCLVEVRALSMQAKEYLYHKHLGNEMPRNNLLIEMLQAKQT 622644C62794826C prox1_human Prospero homeobox protein 1 PROX1 Homeobox prospero-like protein PROX1 protein protein human Homo sapiens Human MPDHDSTALLSRQTKRRRVDIGVKRTVGTASAFFAKARATFFSAMNPQGSEQDVEYSVVQHADGEKSNVLRKLLKRANSYEDAMMPFPGATIISQLLKNNMNKNGGTEPSFQASGLSSTGSEVHQEDICSNSSRDSPPECLSPFGRPTMSQFDMDRLCDEHLRAKRARVENIIRGMSHSPSVALRGNENEREMAPQSVSPRESYRENKRKQKLPQQQQQSFQQLVSARKEQKREERRQLKQQLEDMQKQLRQLQEKFYQIYDSTDSENDEDGNLSEDSMRSEILDARAQDSVGRSDNEMCELDPGQFIDRARALIREQEMAENKPKREGNNKERDHGPNSLQPEGKHLAETLKQELNTAMSQVVDTVVKVFSAKPSRQVPQVFPPLQIPQARFAVNGENHNFHTANQRLQCFGDVIIPNPLDTFGNVQMASSTDQTEALPLVVRKNSSDQSASGPAAGGHHQPLHQSPLSATTGFTTSTFRHPFPLPLMAYPFQSPLGAPSGSFSGKDRASPESLDLTRDTTSLRTKMSSHHLSHHPCSPAHPPSTAEGLSLSLIKSECGDLQDMSEISPYSGSAMQEGLSPNHLKKAKLMFFYTRYPSSNMLKTYFSDVKFNRCITSQLIKWFSNFREFYYIQMEKYARQAINDGVTSTEELSITRDCELYRALNMHYNKANDFEVPERFLEVAQITLREFFNAIIAGKDVDPSWKKAIYKVICKLDSEVPEIFKSPNCLQELLHE D243CEB421B313CA nr0b2_human Nuclear receptor subfamily 0 group B member 2 NR0B2 SHP Orphan nuclear receptor SHP Small heterodimer partner protein protein human Homo sapiens Human MSTSQPGACPCQGAASRPAILYALLSSSLKAVPRPRSRCLCRQHRPVQLCAPHRTCREALDVLAKTVAFLRNLPSFWQLPPQDQRRLLQGCWGPLFLLGLAQDAVTFEVAEAPVPSILKKILLEEPSSSGGSGQLPDRPQPSLAAVQWLQCCLESFWSLELSPKEYACLKGTILFNPDVPGLQAASHIGHLQQEAHWVLCEVLEPWCPAAQGRLTRVLLTASTLKSIPTSLLGDLFFRPIIGDVDIAGLLGDMLLLR DIP protein Q15466 original sequence version: 79 DIP protein Q15466 original sequence version: 113 14BEE2B3FF46154A hnf4a_human Hepatocyte nuclear factor 4-alpha Transcription factor HNF-4 Nuclear receptor subfamily 2 group A member 1 Transcription factor 14 HNF4A HNF4 NR2A1 TCF14 protein protein human Homo sapiens Human MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQMIEQIQFIKLFGMAKIDNLLQEMLLGGSPSDAPHAHHPLHPHLMQEHMGTNVIVANTMPTHLSNGQMCEWPRPRGQAATPETPQPSPPGGSGSEPYKLLPGAVATIVKPLSAIPQPTITKQEVI 5F1309B89D95DCAF nr5a2_human Nuclear receptor subfamily 5 group A member 2 Alpha-1-fetoprotein transcription factor Hepatocytic transcription factor B1-binding factor CYP7A promoter-binding factor Liver receptor homolog 1 NR5A2 B1F CPF FTF protein protein human Homo sapiens Human MSSNSDTGDLQESLKHGLTPIGAGLPDRHGSPIPARGRLVMLPKVETEALGLARSHGEQGQMPENMQVSQFKMVNYSYDEDLEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNNKRYTCIENQNCQIDKTQRKRCPYCRFQKCLSVGMKLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALIRANGLKLEAMSQVIQAMPSDLTISSAIQNIHSASKGLPLNHAALPPTDYDRSPFVTSPISMTMPPHGSLQGYQTYGHFPSRAIKSEYPDPYTSSPESIMGYSYMDSYQTSSPASIPHLILELLKCEPDEPQVQAKIMAYLQQEQANRSKHEKLSTFGLMCKMADQTLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVVHGKEGSIFLVTGQQVDYSIIASQAGATLNNLMSHAQELVAKLRSLQFDQREFVCLKFLVLFSLDVKNLENFQLVEGVQEQVNAALLDYTMCNYPQQTEKFGQLLLRLPEIRAISMQAEEYLYYKHLNGDVPYNNLLIEMLHAKRA 7B07170C075490FE hnf4a-prox1-1 4 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Fig. 1B prox1-nr5a1 3 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Table 1 prox1-hnf4a 3 8 unspecified role unspecified role bait bait n-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q92786:p.[Leu70Ala;Leu73Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain L A Wed May 02 17:26:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu70ala,leu73ala' Tue Jun 30 17:54:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu70Ala;Leu73Ala]' 10 unspecified role unspecified role prey prey af2 domain required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range physical association physical association Table 1, Fig. 2 nr0b2-hnf4a 3 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association Table 1 prox1-hnf4a-1 2 8 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 1A prox1-nr5a2 2 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 8 unspecified role unspecified role bait bait purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1A hnf4a-cyp7a1-3 1 6 unspecified role unspecified role prey prey bare-l bare-ll promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role bait bait association association Fig. 5A Flag-Prox1 was transfected into the HepG2 cells but did not affect HNF4 alpha binding to the CYP7A1 promoter. prox1-cyp7a1-2 1 6 unspecified role unspecified role prey prey bare-l bare-ll promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5A A positive CHIP result was obtained using an anti-FLAG antibody. The authors state:- "Because Prox1 does not bind to DNA, Prox1 must be recruited to CYP7A1 chromatin by HNF4α". However even if true, this does not exclude the possibility that some unknown molecule could be responsible for binding of Flag-Prox1 to the CYP7A1 promoter region. ppargc1a-cyp7a1-2 1 6 unspecified role unspecified role prey prey bare-l bare-ll promoter region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5B Q92786 decreases activity of Q9UBK2 Flag-Prox1 Q92786 inhibits expression at RNA level of ENSG00000101076 P41235 increases expression at RNA level of ENSG00000167910 Q9UBK2 increases expression at RNA level of ENSG00000167910