bhf-ucl Interaction of rat lecithin-cholesterol acyltransferase with rat apolipoprotein A-I and with lecithin-cholesterol vesicles. Interaction of rat lecithin-cholesterol acyltransferase with rat apolipoprotein A-I and with lecithin-cholesterol vesicles. J. Biochem. (0021-924X) 1992 imex curation Furukawa Y., Urano T., Hida Y., Itoh H., Takahashi C., Kimura S. Cardiac - Interactions involving cardiac related proteins shuichi.kimura@jt.com 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom predetermined predetermined participant predetermined Furukawa Y., Urano T., Hida Y., Itoh H., Takahashi C., Kimura S. Cardiac - Interactions involving cardiac related proteins shuichi.kimura@jt.com Accepted 2014-FEB-17 by MGT The elution profiles of the enzymes were determined by standard enzyme assays J. Biochem. (0021-924X) 1992 Only protein-protein interactions imex curation lcat_human Phosphatidylcholine-sterol acyltransferase LCAT Lecithin-cholesterol acyltransferase Phospholipid-cholesterol acyltransferase 1-alkyl-2-acetylglycerophosphocholine esterase Platelet-activating factor acetylhydrolase protein protein human Homo sapiens Human MGPPGSPWQWVTLLLGLLLPPAAPFWLLNVLFPPHTTPKAELSNHTRPVILVPGCLGNQLEAKLDKPDVVNWMCYRKTEDFFTIWLDLNMFLPLGVDCWIDNTRVVYNRSSGLVSNAPGVQIRVPGFGKTYSVEYLDSSKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLEPGQQEEYYRKLAGLVEEMHAAYGKPVFLIGHSLGCLHLLYFLLRQPQAWKDRFIDGFISLGAPWGGSIKPMLVLASGDNQGIPIMSSIKLKEEQRITTTSPWMFPSRMAWPEDHVFISTPSFNYTGRDFQRFFADLHFEEGWYMWLQSRDLLAGLPAPGVEVYCLYGVGLPTPRTYIYDHGFPYTDPVGVLYEDGDDTVATRSTELCGLWQGRQPQPVHLLPLHGIQHLNMVFSNLTLEHINAILLGAYRQGPPASPTASPEPPPPE DIP protein P04180 original sequence version: 91 B315EF118AA7A378 apoa1_human Apolipoprotein A-I APOA1 Apolipoprotein A1 protein protein human Homo sapiens Human MKAAVLTLAVLFLTGSQARHFWQQDEPPQSPWDRVKDLATVYVDVLKDSGRDYVSQFEGSALGKQLNLKLLDNWDSVTSTFSKLREQLGPVTQEFWDNLEKETEGLRQEMSKDLEEVKAKVQPYLDDFQKKWQEEMELYRQKVEPLRAELQEGARQKLHELQEKLSPLGEEMRDRARAHVDALRTHLAPYSDELRQRLAARLEALKENGGARLAEYHAKATEHLSTLSEKAKPALEDLRQGLLPVLESFKVSFLSALEEYTKKLNTQ DIP protein P02647 original sequence version: 116 DIP protein P02647 original sequence version: 125 DIP protein P02647 original sequence version: 147 DIP protein P02647 original sequence version: 162 1A28B8366E620310 apoa1_rat Apolipoprotein A-I Apoa1 Apolipoprotein A1 protein protein rat Rattus norvegicus (Rat) Rat MKAAVLAVALVFLTGCQAWEFWQQDEPQSQWDRVKDFATVYVDAVKDSGRDYVSQFESSTLGKQLNLNLLDNWDTLGSTVGRLQEQLGPVTQEFWANLEKETDWLRNEMNKDLENVKQKMQPHLDEFQEKWNEEVEAYRQKLEPLGTELHKNAKEMQRHLKVVAEEFRDRMRVNADALRAKFGLYSDQMRENLAQRLTEIKNHPTLIEYHTKASDHLKTLGEKAKPALDDLGQGLMPVLEAWKAKIMSMIDEAKKKLNA DIP protein P04639 original sequence version: 68 28538891B2E3A6CD lcat_rat Phosphatidylcholine-sterol acyltransferase Lcat Lecithin-cholesterol acyltransferase Phospholipid-cholesterol acyltransferase 1-alkyl-2-acetylglycerophosphocholine esterase Platelet-activating factor acetylhydrolase protein protein rat Rattus norvegicus (Rat) Rat MGLPGSPWQWVLLLLGLLLPPATSFWLLNVLFPPHTTPKAELSNHTRPVILVPGCMGNRLEAKLDKPNVVNWLCYRKTEDFFTIWLDFNMFLPLGVDCWIDNTRVVYNRSSGHMSNAPGVQIRVPGFGKTYSVEYLDDNKLAGYLHTLVQNLVNNGYVRDETVRAAPYDWRLAPRQQDEYYQKLAGLVEEMYAAYGKPVFLIGHSLGCLHVLHFLLRQPQSWKDHFIDGFISLGAPWGGSIKPMRILASGDNQGIPIMSNIKLREEQRITTTSPWMFPAHHVWPEDHVFISTPNFNYTGQDFERFFADLHFEEGWHMFLQSRDLLAGLPAPGVEVYCLYGVGMPTAHTYIYDHNFPYKDPVAALYEDGDDTVATRSTELCGQWQGRQSQAVHLLPMNGTDHLNMVFSNKTLEHINAILLGAYRHGTPKSPTASLGPPTKE 836BB2D589F72B63 apoa1-lcat-1 1 2 unspecified role unspecified role prey prey human-plasma homo sapiens blood plasma plasma blood plasma 3 unspecified role unspecified role bait bait human Homo sapiens Human direct interaction direct interaction Fig. 3 A, B Human apoA1 was purified from human HDL apoa1-lcat-3 1 4 unspecified role unspecified role bait bait rat Rattus norvegicus (Rat) Rat 5 unspecified role unspecified role prey prey rat-plasma RAT-plasma plasma blood plasma direct interaction direct interaction Fig 3 C, D Rat apoAI was purified from rat HDL . Rat lcat was purified from rat plasma.