bhf-ucl Domains of apoE required for binding to apoE receptor 2 and to phospholipids: implications for the functions of apoE in the brain. Domains of apoE required for binding to apoE receptor 2 and to phospholipids: implications for the functions of apoE in the brain. Biochemistry (0006-2960) 2003 imex curation Li X., Kypreos K., Zanni E.E., Zannis V. Cardiac - Interactions involving cardiac related proteins Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos Chlorocebus aethiops cos cos affinity techniques affinity technology affinity techniques tag visualisation tag visualisation Li X., Kypreos K., Zanni E.E., Zannis V. Cardiac - Interactions involving cardiac related proteins ApoE protein mutants were reconstituted in particles containing POPC (1-palmitoyl-2-oleoyl-L-phosphatidylcholine) Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Accepted 2014-FEB-03 by ORCHARD Biochemistry (0006-2960) 2003 imex curation Domains of apoE required for binding to apoE receptor 2 and to phospholipids: implications for the functions of apoE in the brain. Domains of apoE required for binding to apoE receptor 2 and to phospholipids: implications for the functions of apoE in the brain. Biochemistry (0006-2960) 2003 imex curation Li X., Kypreos K., Zanni E.E., Zannis V. Cardiac - Interactions involving cardiac related proteins Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro affinity techniques affinity technology affinity techniques predetermined predetermined participant predetermined Li X., Kypreos K., Zanni E.E., Zannis V. Cardiac - Interactions involving cardiac related proteins Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Accepted 2014-FEB-03 by ORCHARD The rate of the interaction between apoE and the multilamellar DMPC vesicles was monitored by the change in absorbance at 325nm as the DMPC vesicles were solubilized. Biochemistry (0006-2960) 2003 imex curation Domains of apoE required for binding to apoE receptor 2 and to phospholipids: implications for the functions of apoE in the brain. Domains of apoE required for binding to apoE receptor 2 and to phospholipids: implications for the functions of apoE in the brain. Biochemistry (0006-2960) 2003 imex curation Li X., Kypreos K., Zanni E.E., Zannis V. Cardiac - Interactions involving cardiac related proteins Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. affinity techniques affinity technology affinity techniques tag visualisation tag visualisation Li X., Kypreos K., Zanni E.E., Zannis V. Cardiac - Interactions involving cardiac related proteins ApoE protein mutants were reconstituted in particles containing POPC (1-palmitoyl-2-oleoyl-L-phosphatidylcholine) Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Accepted 2014-FEB-03 by ORCHARD Biochemistry (0006-2960) 2003 imex curation lrp8_human Low-density lipoprotein receptor-related protein 8 APOER2 Apolipoprotein E receptor 2 LRP8 protein protein human Homo sapiens Human MGLPEPGPLRLLALLLLLLLLLLLQLQHLAAAAADPLLGGQGPAKDCEKDQFQCRNERCIPSVWRCDEDDDCLDHSDEDDCPKKTCADSDFTCDNGHCIHERWKCDGEEECPDGSDESEATCTKQVCPAEKLSCGPTSHKCVPASWRCDGEKDCEGGADEAGCATLCAPHEFQCGNRSCLAAVFVCDGDDDCGDGSDERGCADPACGPREFRCGGDGGGACIPERWVCDRQFDCEDRSDEAAELCGRPGPGATSAPAACATASQFACRSGECVHLGWRCDGDRDCKDKSDEADCPLGTCRGDEFQCGDGTCVLAIKHCNQEQDCPDGSDEAGCLQGLNECLHNNGGCSHICTDLKIGFECTCPAGFQLLDQKTCGDIDECKDPDACSQICVNYKGYFKCECYPGYEMDLLTKNCKAAAGKSPSLIFTNRHEVRRIDLVKRNYSRLIPMLKNVVALDVEVATNRIYWCDLSYRKIYSAYMDKASDPKEQEVLIDEQLHSPEGLAVDWVHKHIYWTDSGNKTISVATVDGGRRRTLFSRNLSEPRAIAVDPLRGFMYWSDWGDQAKIEKSGLNGVDRQTLVSDNIEWPNGITLDLLSQRLYWVDSKLHQLSSIDFSGGNRKTLISSTDFLSHPFGIAVFEDKVFWTDLENEAIFSANRLNGLEISILAENLNNPHDIVIFHELKQPRAPDACELSVQPNGGCEYLCLPAPQISSHSPKYTCACPDTMWLGPDMKRCYRAPQSTSTTTLASTMTRTVPATTRAPGTTVHRSTYQNHSTETPSLTAAVPSSVSVPRAPSISPSTLSPATSNHSQHYANEDSKMGSTVTAAVIGIIVPIVVIALLCMSGYLIWRNWKRKNTKSMNFDNPVYRKTTEEEDEDELHIGRTAQIGHVYPAAISSFDRPLWAEPCLGETREPEDPAPALKELFVLPGEPRSQLHQLPKNPLSELPVVKSKRVALSLEDDGLP DIP protein Q14114 original sequence version: 140 DIP protein Q14114 original sequence version: 90 DIP protein Q14114 original sequence version: 97 B10DCF72F62DE71C dimyristoylphosphatidyl choline dimyristoylphosphatidyl choline (1)-(7-myristoyl-4-oxido-10-oxo-3,5,9-trioxa-4-phosphatricosyl)trimethylammonium 4-oxide 1,2-dimyristoyl-sn-glycero-3-phosphatidylcholine 1,2-ditetradecanoyl-sn-glycero-3-phosphocholine 2,3-bis(tetradecanoyloxy)propyl 2-(trimethylammonio)ethyl phosphate [2-({[2,3-bis(tetradecanoyloxy)propyl] phosphonato}oxy)ethyl]trimethylazanium dimyristoyl-phosphatidylcholine dimyristoylphosphatidylcholine DMPC 2,3-bis(tetradecanoyloxy)propyl 2-(trimethylazaniumyl)ethyl phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) CITHEXJVPOWHKC-UHFFFAOYSA-N CITHEXJVPOWHKC-UHFFFAOYSA-N apoe_human Apolipoprotein E APOE protein protein human Homo sapiens Human MKVLWAALLVTFLAGCQAKVEQAVETEPEPELRQQTEWQSGQRWELALGRFWDYLRWVQTLSEQVQEELLSSQVTQELRALMDETMKELKAYKSELEEQLTPVAEETRARLSKELQAAQARLGADMEDVCGRLVQYRGEVQAMLGQSTEELRVRLASHLRKLRKRLLRDADDLQKRLAVYQAGAREGAERGLSAIRERLGPLVEQGRVRAATVGSLAGQPLQERAQAWGERLRARMEEMGSRTRDRLDEVKEQVAEVRAKLEEQAQQIRLQAEAFQARLKSWFEPLVEDMQRQWAGLVEKVQAAVGTSAAPVPSDNH DIP protein P02649 original sequence version: 197 DIP protein P02649 original sequence version: 146 91AFC04210A30689 lrp8-apoe-1 3 4 unspecified role unspecified role bait bait crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. They use an LDL receptor deficient crigr-cho cell mutant called ldlA to express apoER2. 6 unspecified role unspecified role prey prey i125 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ser112cys_ his158cys natural, standard, encoded residue natural, standard, encoded residue certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human-htb13 Human astrocytoma-derived cells (HTB13) htb13 human astrocytoma-derived cells (HTB13) ApoE 2 (Ser112 Cys / His 158 Cys) is a natural ApoE isoform physical association physical association Fig.5A, 6A Table 2 The specific binding curve and binding parameters were determined by substracting the binding values to the ldlA cell for every experimental point from the corresponding binding values to ldlA apoER2 cells dimyristoylphosphatidyl_choline-apoe 2 6 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain The carboxy terminal helices of apoE between residues 229 and 299 are critical for efficient interaction of apoE with phospholipids ser112arg_ his158arg natural, standard, encoded residue natural, standard, encoded residue certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human-htb13 Human astrocytoma-derived cells (HTB13) htb13 human astrocytoma-derived cells (HTB13) 5 unspecified role unspecified role bait bait direct interaction direct interaction Fig. 4 There was a small but reproducible increase in the rate of initial solubilization of the multilamellar DMPC vesicles by apoE4 as compared to apoE2, apoE3 and apoE4R158M. lrp8-apoe-2 1 4 unspecified role unspecified role bait bait chlae-cos Chlorocebus aethiops cos cos They use an LDL receptor deficient crigr-cho cell mutant called ldlA to express apoER2. 6 unspecified role unspecified role prey prey i125 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ser112cys_ his158cys natural, non-standard encoded residue natural, non-standard encoded residue certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human-htb13 Human astrocytoma-derived cells (HTB13) htb13 human astrocytoma-derived cells (HTB13) ApoE 2 (Ser112 Cys / His 158 Cys) is a natural ApoE isoform physical association physical association Fig.5B The specific binding curve and binding parameters were determined by substracting the binding values to the untransfected COS-M6 cells for every experimental point from the corresponding binding values to transiently transfected COS-M6 cells