bhf-ucl Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel. Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel. Science (0036-8075) 2002 imex curation Marx S.O., Kurokawa J., Reiken S., Motoike H., D'Armiento J., Marks A.R., Kass R.S. Cardiac - Interactions involving cardiac related proteins no Have not annotated Fig1 B as I am not convinced of the specificity of binding of microcystin- or cAMP-Sepharose beads. cardiac muscle homogenates from human or TG+(hKCNQ1-hKCNE1) mouse hearts hKCNQ1 cDNA template was amplified by PCR and cloned into pGEX-4T1, -4T2 or -4T3 for expression as GST fusion proteins in either DH5α or BL21 cells and affinity-purified with glutathione 4B sepharose. Protein identifier for PKA (catalytic subunit), RII (PKA regulatory subunit), and PP1 (catalytic subunit of PP1) unknown so not annotated. Fig 1D is Representative of pulldown with human and mouse cardiac tissue Fig 2D no information about source of yotiao used for transfection rsk20@columbia.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation mouse-heart Mus musculus heart [Cardiac; Cardiac tissue] heart Heart [Cardiac; Cardiac tissue] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Marx S.O., Kurokawa J., Reiken S., Motoike H., D'Armiento J., Marks A.R., Kass R.S. Cardiac - Interactions involving cardiac related proteins no Have not annotated Fig1 B as I am not convinced of the specificity of binding of microcystin- or cAMP-Sepharose beads. cardiac muscle homogenates from human or TG+(hKCNQ1-hKCNE1) mouse hearts Have not annotated Fig1 B as I am not convinced of the specificity of binding of microcystin- or cAMP-Sepharose beads. cardiac muscle homogenates from human or TG+(hKCNQ1-hKCNE1) mouse hearts hKCNQ1 cDNA template was amplified by PCR and cloned into pGEX-4T1, -4T2 or -4T3 for expression as GST fusion proteins in either DH5α or BL21 cells and affinity-purified with glutathione 4B sepharose. Protein identifier for PKA (catalytic subunit), RII (PKA regulatory subunit), and PP1 (catalytic subunit of PP1) unknown so not annotated. Fig 1D is Representative of pulldown with human and mouse cardiac tissue Fig 2D no information about source of yotiao used for transfection rsk20@columbia.edu Accepted 2013-NOV-07 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Science (0036-8075) 2002 imex curation Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel. Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel. Science (0036-8075) 2002 imex curation Marx S.O., Kurokawa J., Reiken S., Motoike H., D'Armiento J., Marks A.R., Kass R.S. Cardiac - Interactions involving cardiac related proteins no Have not annotated Fig1 B as I am not convinced of the specificity of binding of microcystin- or cAMP-Sepharose beads. cardiac muscle homogenates from human or TG+(hKCNQ1-hKCNE1) mouse hearts hKCNQ1 cDNA template was amplified by PCR and cloned into pGEX-4T1, -4T2 or -4T3 for expression as GST fusion proteins in either DH5α or BL21 cells and affinity-purified with glutathione 4B sepharose. Protein identifier for PKA (catalytic subunit), RII (PKA regulatory subunit), and PP1 (catalytic subunit of PP1) unknown so not annotated. Fig 1D is Representative of pulldown with human and mouse cardiac tissue Fig 2D no information about source of yotiao used for transfection rsk20@columbia.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Marx S.O., Kurokawa J., Reiken S., Motoike H., D'Armiento J., Marks A.R., Kass R.S. Cardiac - Interactions involving cardiac related proteins no Have not annotated Fig1 B as I am not convinced of the specificity of binding of microcystin- or cAMP-Sepharose beads. cardiac muscle homogenates from human or TG+(hKCNQ1-hKCNE1) mouse hearts hKCNQ1 cDNA template was amplified by PCR and cloned into pGEX-4T1, -4T2 or -4T3 for expression as GST fusion proteins in either DH5α or BL21 cells and affinity-purified with glutathione 4B sepharose. Protein identifier for PKA (catalytic subunit), RII (PKA regulatory subunit), and PP1 (catalytic subunit of PP1) unknown so not annotated. Fig 1D is Representative of pulldown with human and mouse cardiac tissue Fig 2D no information about source of yotiao used for transfection rsk20@columbia.edu Accepted 2013-NOV-07 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Science (0036-8075) 2002 imex curation Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel. Requirement of a macromolecular signaling complex for beta adrenergic receptor modulation of the KCNQ1-KCNE1 potassium channel. Science (0036-8075) 2002 imex curation Marx S.O., Kurokawa J., Reiken S., Motoike H., D'Armiento J., Marks A.R., Kass R.S. Cardiac - Interactions involving cardiac related proteins no Have not annotated Fig1 B as I am not convinced of the specificity of binding of microcystin- or cAMP-Sepharose beads. cardiac muscle homogenates from human or TG+(hKCNQ1-hKCNE1) mouse hearts hKCNQ1 cDNA template was amplified by PCR and cloned into pGEX-4T1, -4T2 or -4T3 for expression as GST fusion proteins in either DH5α or BL21 cells and affinity-purified with glutathione 4B sepharose. Protein identifier for PKA (catalytic subunit), RII (PKA regulatory subunit), and PP1 (catalytic subunit of PP1) unknown so not annotated. Fig 1D is Representative of pulldown with human and mouse cardiac tissue Fig 2D no information about source of yotiao used for transfection rsk20@columbia.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Marx S.O., Kurokawa J., Reiken S., Motoike H., D'Armiento J., Marks A.R., Kass R.S. Cardiac - Interactions involving cardiac related proteins no Have not annotated Fig1 B as I am not convinced of the specificity of binding of microcystin- or cAMP-Sepharose beads. cardiac muscle homogenates from human or TG+(hKCNQ1-hKCNE1) mouse hearts hKCNQ1 cDNA template was amplified by PCR and cloned into pGEX-4T1, -4T2 or -4T3 for expression as GST fusion proteins in either DH5α or BL21 cells and affinity-purified with glutathione 4B sepharose. Protein identifier for PKA (catalytic subunit), RII (PKA regulatory subunit), and PP1 (catalytic subunit of PP1) unknown so not annotated. Fig 1D is Representative of pulldown with human and mouse cardiac tissue Fig 2D no information about source of yotiao used for transfection rsk20@columbia.edu Accepted 2013-NOV-07 by ORCHARD 22-Nov-2013: Contacted by IntAct-Help. Science (0036-8075) 2002 imex curation kcnq1_human Potassium voltage-gated channel subfamily KQT member 1 KCNQ1 KCNA8 KCNA9 KVLQT1 Voltage-gated potassium channel subunit Kv7.1 IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1 KQT-like 1 protein protein human Homo sapiens Human MAAASSPPRAERKRWGWGRLPGARRGSAGLAKKCPFSLELAEGGPAGGALYAPIAPGAPGPAPPASPAAPAAPPVASDLGPRPPVSLDPRVSIYSTRRPVLARTHVQGRVYNFLERPTGWKCFVYHFAVFLIVLVCLIFSVLSTIEQYAALATGTLFWMEIVLVVFFGTEYVVRLWSAGCRSKYVGLWGRLRFARKPISIIDLIVVVASMVVLCVGSKGQVFATSAIRGIRFLQILRMLHVDRQGGTWRLLGSVVFIHRQELITTLYIGFLGLIFSSYFVYLAEKDAVNESGRVEFGSYADALWWGVVTVTTIGYGDKVPQTWVGKTIASCFSVFAISFFALPAGILGSGFALKVQQKQRQKHFNRQIPAAASLIQTAWRCYAAENPDSSTWKIYIRKAPRSHTLLSPSPKPKKSVVVKKKKFKLDKDNGVTPGEKMLTVPHITCDPPEERRLDHFSVDGYDSSVRKSPTLLEVSMPHFMRTNSFAEDLDLEGETLLTPITHISQLREHHRATIKVIRRMQYFVAKKKFQQARKPYDVRDVIEQYSQGHLNLMVRIKELQRRLDQSIGKPSLFISVSEKSKDRGSNTIGARLNRVEDKVTQLDQRLALITDMLHQLLSLHGGSTPGSGGPPREGGAHITQPCGSGGSVDPELFLPSNTLPTYEQLTVPRRGPDEGS This sequence has been withdrawn from Uniprot. ADFCA9E2B9763B21 ADFCA9E2B9763B21 akap9_human A-kinase anchor protein 9 Protein kinase A-anchoring protein 9 A-kinase anchor protein 450 kDa A-kinase anchor protein 350 kDa AKAP 120-like protein Protein hyperion Protein yotiao Centrosome- and Golgi-localized PKN-associated protein AKAP9 AKAP350 AKAP450 KIAA0803 protein protein human Homo sapiens Human MEDEERQKKLEAGKAKLAQFRQRKAQSDGQSPSKKQKKKRKTSSSKHDVSAHHDLNIDQSQCNEMYINSSQRVESTVIPESTIMRTLHSGEITSHEQGFSVELESEISTTADDCSSEVNGCSFVMRTGKPTNLLREEEFGVDDSYSEQGAQDSPTHLEMMESELAGKQHEIEELNRELEEMRVTYGTEGLQQLQEFEAAIKQRDGIITQLTANLQQARREKDETMREFLELTEQSQKLQIQFQQLQASETLRNSTHSSTAADLLQAKQQILTHQQQLEEQDHLLEDYQKKKEDFTMQISFLQEKIKVYEMEQDKKVENSNKEEIQEKETIIEELNTKIIEEEKKTLELKDKLTTADKLLGELQEQIVQKNQEIKNMKLELTNSKQKERQSSEEIKQLMGTVEELQKRNHKDSQFETDIVQRMEQETQRKLEQLRAELDEMYGQQIVQMKQELIRQHMAQMEEMKTRHKGEMENALRSYSNITVNEDQIKLMNVAINELNIKLQDTNSQKEKLKEELGLILEEKCALQRQLEDLVEELSFSREQIQRARQTIAEQESKLNEAHKSLSTVEDLKAEIVSASESRKELELKHEAEVTNYKIKLEMLEKEKNAVLDRMAESQEAELERLRTQLLFSHEEELSKLKEDLEIEHRINIEKLKDNLGIHYKQQIDGLQNEMSQKIETMQFEKDNLITKQNQLILEISKLKDLQQSLVNSKSEEMTLQINELQKEIEILRQEEKEKGTLEQEVQELQLKTELLEKQMKEKENDLQEKFAQLEAENSILKDEKKTLEDMLKIHTPVSQEERLIFLDSIKSKSKDSVWEKEIEILIEENEDLKQQCIQLNEEIEKQRNTFSFAEKNFEVNYQELQEEYACLLKVKDDLEDSKNKQELEYKSKLKALNEELHLQRINPTTVKMKSSVFDEDKTFVAETLEMGEVVEKDTTELMEKLEVTKREKLELSQRLSDLSEQLKQKHGEISFLNEEVKSLKQEKEQVSLRCRELEIIINHNRAENVQSCDTQVSSLLDGVVTMTSRGAEGSVSKVNKSFGEESKIMVEDKVSFENMTVGEESKQEQLILDHLPSVTKESSLRATQPSENDKLQKELNVLKSEQNDLRLQMEAQRICLSLVYSTHVDQVREYMENEKDKALCSLKEELIFAQEEKIKELQKIHQLELQTMKTQETGDEGKPLHLLIGKLQKAVSEECSYFLQTLCSVLGEYYTPALKCEVNAEDKENSGDYISENEDPELQDYRYEVQDFQENMHTLLNKVTEEYNKLLVLQTRLSKIWGQQTDGMKLEFGEENLPKEETEFLSIHSQMTNLEDIDVNHKSKLSSLQDLEKTKLEEQVQELESLISSLQQQLKETEQNYEAEIHCLQKRLQAVSESTVPPSLPVDSVVITESDAQRTMYPGSCVKKNIDGTIEFSGEFGVKEETNIVKLLEKQYQEQLEEEVAKVIVSMSIAFAQQTELSRISGGKENTASSKQAHAVCQQEQHYFNEMKLSQDQIGFQTFETVDVKFKEEFKPLSKELGEHGKEILLSNSDPHDIPESKDCVLTISEEMFSKDKTFIVRQSIHDEISVSSMDASRQLMLNEEQLEDMRQELVRQYQEHQQATELLRQAHMRQMERQREDQEQLQEEIKRLNRQLAQRSSIDNENLVSERERVLLEELEALKQLSLAGREKLCCELRNSSTQTQNGNENQGEVEEQTFKEKELDRKPEDVPPEILSNERYALQKANNRLLKILLEVVKTTAAVEETIGRHVLGILDRSSKSQSSASLIWRSEAEASVKSCVHEEHTRVTDESIPSYSGSDMPRNDINMWSKVTEEGTELSQRLVRSGFAGTEIDPENEELMLNISSRLQAAVEKLLEAISETSSQLEHAKVTQTELMRESFRQKQEATESLKCQEELRERLHEESRAREQLAVELSKAEGVIDGYADEKTLFERQIQEKTDIIDRLEQELLCASNRLQELEAEQQQIQEERELLSRQKEAMKAEAGPVEQQLLQETEKLMKEKLEVQCQAEKVRDDLQKQVKALEIDVEEQVSRFIELEQEKNTELMDLRQQNQALEKQLEKMRKFLDEQAIDREHERDVFQQEIQKLEQQLKVVPRFQPISEHQTREVEQLANHLKEKTDKCSELLLSKEQLQRDIQERNEEIEKLEFRVRELEQALLVSADTFQKVEDRKHFGAVEAKPELSLEVQLQAERDAIDRKEKEITNLEEQLEQFREELENKNEEVQQLHMQLEIQKKESTTRLQELEQENKLFKDDMEKLGLAIKESDAMSTQDQHVLFGKFAQIIQEKEVEIDQLNEQVTKLQQQLKITTDNKVIEEKNELIRDLETQIECLMSDQECVKRNREEEIEQLNEVIEKLQQELANIGQKTSMNAHSLSEEADSLKHQLDVVIAEKLALEQQVETANEEMTFMKNVLKETNFKMNQLTQELFSLKRERESVEKIQSIPENSVNVAIDHLSKDKPELEVVLTEDALKSLENQTYFKSFEENGKGSIINLETRLLQLESTVSAKDLELTQCYKQIKDMQEQGQFETEMLQKKIVNLQKIVEEKVAAALVSQIQLEAVQEYAKFCQDNQTISSEPERTNIQNLNQLREDELGSDISALTLRISELESQVVEMHTSLILEKEQVEIAEKNVLEKEKKLLELQKLLEGNEKKQREKEKKRSPQDVEVLKTTTELFHSNEESGFFNELEALRAESVATKAELASYKEKAEKLQEELLVKETNMTSLQKDLSQVRDHLAEAKEKLSILEKEDETEVQESKKACMFEPLPIKLSKSIASQTDGTLKISSSNQTPQILVKNAGIQINLQSECSSEEVTEIISQFTEKIEKMQELHAAEILDMESRHISETETLKREHYVAVQLLKEECGTLKAVIQCLRSKEGSSIPELAHSDAYQTREICSSDSGSDWGQGIYLTHSQGFDIASEGRGEESESATDSFPKKIKGLLRAVHNEGMQVLSLTESPYSDGEDHSIQQVSEPWLEERKAYINTISSLKDLITKMQLQREAEVYDSSQSHESFSDWRGELLLALQQVFLEERSVLLAAFRTELTALGTTDAVGLLNCLEQRIQEQGVEYQAAMECLQKADRRSLLSEIQALHAQMNGRKITLKREQESEKPSQELLEYNIQQKQSQMLEMQVELSSMKDRATELQEQLSSEKMVVAELKSELAQTKLELETTLKAQHKHLKELEAFRLEVKDKTDEVHLLNDTLASEQKKSRELQWALEKEKAKLGRSEERDKEELEDLKFSLESQKQRNLQLNLLLEQQKQLLNESQQKIESQRMLYDAQLSEEQGRNLELQVLLESEKVRIREMSSTLDRERELHAQLQSSDGTGQSRPPLPSEDLLKELQKQLEEKHSRIVELLNETEKYKLDSLQTRQQMEKDRQVHRKTLQTEQEANTEGQKKMHELQSKVEDLQRQLEEKRQQVYKLDLEGQRLQGIMQEFQKQELEREEKRESRRILYQNLNEPTTWSLTSDRTRNWVLQQKIEGETKESNYAKLIEMNGGGTGCNHELEMIRQKLQCVASKLQVLPQKASERLQFETADDEDFIWVQENIDEIILQLQKLTGQQGEEPSLVSPSTSCGSLTERLLRQNAELTGHISQLTEEKNDLRNMVMKLEEQIRWYRQTGAGRDNSSRFSLNGGANIEAIIASEKEVWNREKLTLQKSLKRAEAEVYKLKAELRNDSLLQTLSPDSEHVTLKRIYGKYLRAESFRKALIYQKKYLLLLLGGFQECEDATLALLARMGGQPAFTDLEVITNRPKGFTRFRSAVRVSIAISRMKFLVRRWHRVTGSVSININRDGFGLNQGAEKTDSFYHSSGGLELYGEPRHTTYRSRSDLDYIRSPLPFQNRYPGTPADFNPGSLACSQLQNYDPDRALTDYITRLEALQRRLGTIQSGSTTQFHAGMRR DIP protein Q99996 original sequence version: 154 81761B4341430CDF akap9_mouse A-kinase anchor protein 9 Akap9 Kiaa0803 Protein kinase A-anchoring protein 9 protein protein mouse Mus musculus Mouse MEDEERQRKLAAGKAKLARFRQRKAQYDGDIPKKQKKKRTSSSKHDSSLHTDQQSGELCSESSQRVDLAGNPDCSGPERKHGQVFSAEPESEISTTADECSSEINGCNSVMKPRKPTDPLREEEFSLDDSSSEQGAQSSQTCLQMVEKELAEKQHDIEELTQELEEMRASFGTEGLKQLQEFEAAIKQRDGIITQLTANLQQARREKDDTMVEFLELTEQSQKLQIQFQHLQANETLQNSTLSRTATDLLQAKRQIFTQQQQLQDYQKKEEDLQAQISFLQEKLRAFEMEKDRKIENLNAKEIQEKQALIDELNTRVVEEEKKTVELKNKVTTADELLGGLHEQLTQRNQEIQSLKLELGNSQQNERKCSEEIKELMRTVEELQKRNLKDSWLETSAVRRVEQETQRKLSHLQAELDEMYGKQIVQMKQELINQHMSQIEELKSQHKREMENTLKSDTNAAISKEQVNLMNAAINELNVRLQETHAQKEELKGELGVVLGEKSALQSQSNDLLEEVRFLREQVQKARQTIAEQENRLSEARKSLSTVEDLKAEIVAASESRKELELKHEAEITNYKIKLEMLEKEKNAVLDRMAESQEAELERLRTQPLFSHEEELSKLKEDLEVEHRINIEKLKDNLGIHYKQQIDGLQNEMNRKMESMQCETDNLITQQNQLILENSKLRDLQECLVNSKSEEMNLQINELQKEIEILKQEEKEKGTLEQEVQELQLKTEQLEKQLKEKEDDLQEKCAQLDAENNILKEEKRVLEDKLKMYSPSEQEERSIAVDPSTSKLADSRWQKEVAMLRKETEDLQQQCLYLNEEIEKQRNTFAFAEKNFEVNYQELQREYTCLLKIRDDLEATQTKQALEYESKLRALEEELLSKRGNPXAPKGKSSGIFPSETLEIGEVVEKDTTELMEKLEVTKREKLELSEKVSGLSEQLKQTHCTINSLSAEXRALKQEKEQLLLRCGELELLANPSGTENAAVCPVQMSSYQAGLVMGKVGDSGGSISKISKDLAEESKPMIEDKIPFKESGREQLLLPTRAQEPSHATVEPCESEKLQQELHALKAEQDDLRLQMEAQRICLFVVYSTHADQVRAHMEKEREEALCSLKDELISAQQKKIDELHKMHQCQLQNVKIQETGDEPLQVLIERLQKAVSEKCFHISKTLNNVFDECYTPLKCEMNIEEKENSGVYTSQNQSPELQEYRYEVQDFQESMQVLLGKVTEECRKLSGLQTRLGKIHEQQTDGVALEFAEQNAAEEEAGLLSGCSQSALQSTDVSLESKVSSLPASEKNRECERQVQELQSPVAAGQLQLTETEASHRAEIECLQQRLEAASEAPVQPSLSIDSVVFKGSGAQKPVYCGSCLREYVDGTAKFSDRFEVRQETNMVNLMEKQYQERLEEEIAKVIVSMSIAFAQQTELSRLSEGKENTIQSEQAHTLCSQNKHQLNDITSQSQVGLQTFEATDKBFKEEFKPLSKELGEYRKAVPLSSHDDLDDILKSEEHGLAISEEIFSKDETFIVRKSMHDEVLVSSMDTSRQLILNEQLEDMRQELVRQYEEHQQATEMLRQAHMQQMERQREDQEQLQEEIKRLNEQLTQKSSIDTEHVVSERERVLLEELEALKQLPLAGRKELCCELRHSSTQTQDGHDDQEVEEQTLKDKTLERSPEDALLDRNLSNERYALKKANNRLLKILLEVVKTTSAAEETIGRHVLGILDRSSKGQTASSLLWRSEADASATTCAPEDCARAMDESIPSYPGTAIATHDSIWSKVTEEGAELSQRLVRSGFAGPVIDPENEELMLNISSRLQAAVEKLLEAISETNTQLEHAKVTQTELMRESFRQKQEATESLHCLEELRERLQEESRAREQLAEELNKAESVIDGYSDEKTLFERQIQEKTDIIEHLEQEVLCMNNRLQELESDQRRVEEERQLLCRQREAMRAEAGPVEQQFLQETEKLMKEKLEVQCQAEKVRGDLQKQVKALEIDVEEQVSRFIELEQEKNAELTDLRQQSQALEKQLEKMRKFLDEQAIDREHERDVFQQEIQKLEHQLKAAPRIQPVSEHQAREVEQLTNHLKEKTDRCSELLLSKEQLQRDIQERNEEIEKLECRVRELEQALLASAEPFPKVEDQKRSGAVAADPELSLEVQLQAERDATDRKQKEITNLEEQLEQFREELENKNDEVQELLMQLEIQRKESTTRLQELQQENRLFKDEIEKLGFAMKESDSVSTRDQPMLFGKFAQLIQEKEIEIDRLNEQFIKLQQQLKLTTDNKVIEEQKEQIQDLETQIERLMSEREHEKKQREEEVEQLTGVVEKLQQEVVSTEQQREGARTLPEDEESFKHQLDKVTAEKLVLEQQVETTNQVMTHMNNVLKEINFKMDQITQSLCNLNKECASNEELPSLPKESVHMTVHELGSDNLQPEDAPAQDVTKPLEKQTSLTRLQESPEASRTQEIESLASSVGAKDVELTQCREQTETIQEQAQSETDRLQKKLTDLQRSLEKFAAALVSQVQMEAAQEYVPFHQEKQPVSSAPGSTDIQNANGLTGASTESLIPTVTLRLAEVESRVAEVHSGTMSEKLVGIVGGNASETEKRVIELQKLLEEAEERPEEGGEQSSRDGEVRESYMTSLQKDLGQVKDPLTEAKEKLSYSLEKEKRTGEQESREAPIPEPPSVEVGGCSGLTERTDKVSSSGNQTLQILLRDAAIQTDLQSESSQEEVRDTINQLTKKMEHIQELHAAEILDMESRHILETESLKKEHYVAIQLLTKECETLKEMTQCLRCKEGSSIPELADSVAYQSREVYSSDSESDWGQSQGFDTAIEGREEGETSADLFPKKIKGLVKAVHSEGMQVLSLSSPLCDDGEDRSIQQLSESWLKERQAYLNTISSLKDLISKMQVRRETEVYDRCHLSDWRGELLLACQRVFIKERSVLLATFQTELTSLSTRDVDGLLNSLEQRIQEQGIEYHTAMDCLQKADRRSLLAEIEDLRAQINGGKMTLEREQGTEKSSQELLDCSMQQKQSLEMQLELSSLRDRAAELQEQLSSEKMVVAELKSELAQAKLELGTTLKAQHKRLKELEAFRSEVKEKTDEIHFLSDTLAREQKNSLELQWALEKEKARSGHHEEREKEELEDLKFSLEDQKRRNTQLNLLLEQQKQLLNESQQKIESQKMLHDAQLSEEQGRNLGLQALLESEQVRIQEMKSTLDKERELYAQLQSREDGGQPPPALPSEDLLKELQKQLEEKHSRIVELLSETEKYKLDSLQTRQQMEKDRQVHQKTLQTEQEANTQGQKKMQELQSKVEELQRQLQEKRQQVYKLDLEGKRLQGLMQEFQKQELEPEEKPGSRGLVDQNLNEPATWNFTDDRTRNWVLQQKMGEAKDRNFTKLIEINGGELDHNHDLEMIRQTLQHVASKLQHVAQKACSRLQFETAGDDAFIWIQENIDGIILQLQKLTGQPGDEHSLGPPSSSCGSLTESLMRQNTELTRLINQLTEEKNTLRSIVIKLEELNRCYWHTGASRDCCSRFSFIDPADIEAIIASEKEVWNREKLSLQKALKRAEAKVYKLKAELRNDALLRNLGPDTDHAALQKIYNKYLRASSFRKALIYQKKYLLLLLGGFQECEDVTLGVLARMGGHLALKDSKTITNHPKAFSRFRSAVRVSIAISRMKFLVRRWQQVTSTSSININRDGFGLSPGIEKTDPFYHSPGGLELYGEPRHTMYRSRFDLDYPRSLLPLQNRYPGTPGDLNSISMASSQLHQYNPDKSLTDYVTRLEALRRRLGAIQSGSTTQFHFGMRR CA30D11FF5696124 kcnq1-akap9-4 1 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey association association Fig 1C, 2C In Figure 1C anti-PKA (catalytic subunit), anti-RII (PKA regulatory subunit), anti-PP1 (catalytic subunit of PP1) not specific enough to annotate bound protein. In Figure 2C anti-PKA, and anti-PP1 not specific enough to annotate bound protein. kcnq1-akap9-2 3 5 unspecified role unspecified role prey prey human-heart Homo sapiens heart Heart [Cardiac; Cardiac tissue] 4 unspecified role unspecified role bait bait purified purified region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P51787:p.[Leu602Ala;Ile609Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 17:44:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu602ala,ile609ala' Tue Jun 30 16:31:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu602Ala;Ile609Ala]' escherichia coli dh5[alpha] escherichia coli dh5[alpha] association association Fig 1D and results text In Figure 1D anti-PKA, and anti-PP1 not specific enough to annotate bound protein. kcnq1-akap9-3 2 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P51787:p.[Leu602Ala;Ile609Ala] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain I A Wed May 02 15:12:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu602ala,ile609ala' Tue Jun 30 18:52:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu602Ala;Ile609Ala]' escherichia coli dh5[alpha] escherichia coli dh5[alpha] 6 unspecified role unspecified role prey prey mouse-heart Mus musculus heart [Cardiac; Cardiac tissue] heart Heart [Cardiac; Cardiac tissue] association association Fig 1D and results text, 2C In Figure 1D anti-PKA, and anti-PP1 not specific enough to annotate bound protein. In Figure 2C anti-PKA, and anti-PP1 not specific enough to annotate bound protein.