bhf-ucl The Q allele variant (GLN121) of membrane glycoprotein PC-1 interacts with the insulin receptor and inhibits insulin signaling more effectively than the common K allele variant (LYS121). The Q allele variant (GLN121) of membrane glycoprotein PC-1 interacts with the insulin receptor and inhibits insulin signaling more effectively than the common K allele variant (LYS121). Diabetes (0012-1797) 2001 imex curation Costanzo B.V., Trischitta V., Di Paola R., Spampinato D., Pizzuti A., Vigneri R., Frittitta L. Cardiac - Interactions involving cardiac related proteins did not curate experiments in figure 4 and 5 (enzyme assay) as IR and IRS-1 proteins were not purified beyond an initial IP. segmeint@mbox.unict.it 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Costanzo B.V., Trischitta V., Di Paola R., Spampinato D., Pizzuti A., Vigneri R., Frittitta L. Cardiac - Interactions involving cardiac related proteins did not curate experiments in figure 4 and 5 (enzyme assay) as IR and IRS-1 proteins were not purified beyond an initial IP. segmeint@mbox.unict.it Accepted 2013-NOV-25 by ORCHARD Diabetes (0012-1797) 2001 imex curation The Q allele variant (GLN121) of membrane glycoprotein PC-1 interacts with the insulin receptor and inhibits insulin signaling more effectively than the common K allele variant (LYS121). The Q allele variant (GLN121) of membrane glycoprotein PC-1 interacts with the insulin receptor and inhibits insulin signaling more effectively than the common K allele variant (LYS121). Diabetes (0012-1797) 2001 imex curation Costanzo B.V., Trischitta V., Di Paola R., Spampinato D., Pizzuti A., Vigneri R., Frittitta L. Cardiac - Interactions involving cardiac related proteins did not curate experiments in figure 4 and 5 (enzyme assay) as IR and IRS-1 proteins were not purified beyond an initial IP. segmeint@mbox.unict.it 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip polyclonal western polyclonal antibody western blot polyclonal western Costanzo B.V., Trischitta V., Di Paola R., Spampinato D., Pizzuti A., Vigneri R., Frittitta L. Cardiac - Interactions involving cardiac related proteins did not curate experiments in figure 4 and 5 (enzyme assay) as IR and IRS-1 proteins were not purified beyond an initial IP. segmeint@mbox.unict.it Accepted 2013-NOV-25 by ORCHARD Diabetes (0012-1797) 2001 imex curation insr_human Insulin receptor INSR protein protein human Homo sapiens Human MATGGRRGAAAAPLLVAVAALLLGAAGHLYPGEVCPGMDIRNNLTRLHELENCSVIEGHLQILLMFKTRPEDFRDLSFPKLIMITDYLLLFRVYGLESLKDLFPNLTVIRGSRLFFNYALVIFEMVHLKELGLYNLMNITRGSVRIEKNNELCYLATIDWSRILDSVEDNYIVLNKDDNEECGDICPGTAKGKTNCPATVINGQFVERCWTHSHCQKVCPTICKSHGCTAEGLCCHSECLGNCSQPDDPTKCVACRNFYLDGRCVETCPPPYYHFQDWRCVNFSFCQDLHHKCKNSRRQGCHQYVIHNNKCIPECPSGYTMNSSNLLCTPCLGPCPKVCHLLEGEKTIDSVTSAQELRGCTVINGSLIINIRGGNNLAAELEANLGLIEEISGYLKIRRSYALVSLSFFRKLRLIRGETLEIGNYSFYALDNQNLRQLWDWSKHNLTITQGKLFFHYNPKLCLSEIHKMEEVSGTKGRQERNDIALKTNGDQASCENELLKFSYIRTSFDKILLRWEPYWPPDFRDLLGFMLFYKEAPYQNVTEFDGQDACGSNSWTVVDIDPPLRSNDPKSQNHPGWLMRGLKPWTQYAIFVKTLVTFSDERRTYGAKSDIIYVQTDATNPSVPLDPISVSNSSSQIILKWKPPSDPNGNITHYLVFWERQAEDSELFELDYCLKGLKLPSRTWSPPFESEDSQKHNQSEYEDSAGECCSCPKTDSQILKELEESSFRKTFEDYLHNVVFVPRKTSSGTGAEDPRPSRKRRSLGDVGNVTVAVPTVAAFPNTSSTSVPTSPEEHRPFEKVVNKESLVISGLRHFTGYRIELQACNQDTPEERCSVAAYVSARTMPEAKADDIVGPVTHEIFENNVVHLMWQEPKEPNGLIVLYEVSYRRYGDEELHLCVSRKHFALERGCRLRGLSPGNYSVRIRATSLAGNGSWTEPTYFYVTDYLDVPSNIAKIIIGPLIFVFLFSVVIGSIYLFLRKRQPDGPLGPLYASSNPEYLSASDVFPCSVYVPDEWEVSREKITLLRELGQGSFGMVYEGNARDIIKGEAETRVAVKTVNESASLRERIEFLNEASVMKGFTCHHVVRLLGVVSKGQPTLVVMELMAHGDLKSYLRSLRPEAENNPGRPPPTLQEMIQMAAEIADGMAYLNAKKFVHRDLAARNCMVAHDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMAPESLKDGVFTTSSDMWSFGVVLWEITSLAEQPYQGLSNEQVLKFVMDGGYLDQPDNCPERVTDLMRMCWQFNPKMRPTFLEIVNLLKDDLHPSFPEVSFFHSEENKAPESEELEMEFEDMENVPLDRSSHCQREEAGGRDGGSSLGFKRSYEEHIPYTHMNGGKKNGRILTLPRSNPS 709A955660739066 enpp1_human Ectonucleotide pyrophosphatase/phosphodiesterase family member 1 ENPP1 M6S1 NPPS PC1 PDNP1 Membrane component chromosome 6 surface marker 1 Phosphodiesterase I/nucleotide pyrophosphatase 1 Plasma-cell membrane glycoprotein PC-1 protein protein human Homo sapiens Human MERDGCAGGGSRGGEGGRAPREGPAGNGRDRGRSHAAEAPGDPQAAASLLAPMDVGEEPLEKAARARTAKDPNTYKVLSLVLSVCVLTTILGCIFGLKPSCAKEVKSCKGRCFERTFGNCRCDAACVELGNCCLDYQETCIEPEHIWTCNKFRCGEKRLTRSLCACSDDCKDKGDCCINYSSVCQGEKSWVEEPCESINEPQCPAGFETPPTLLFSLDGFRAEYLHTWGGLLPVISKLKKCGTYTKNMRPVYPTKTFPNHYSIVTGLYPESHGIIDNKMYDPKMNASFSLKSKEKFNPEWYKGEPIWVTAKYQGLKSGTFFWPGSDVEINGIFPDIYKMYNGSVPFEERILAVLQWLQLPKDERPHFYTLYLEEPDSSGHSYGPVSSEVIKALQRVDGMVGMLMDGLKELNLHRCLNLILISDHGMEQGSCKKYIYLNKYLGDVKNIKVIYGPAARLRPSDVPDKYYSFNYEGIARNLSCREPNQHFKPYLKHFLPKRLHFAKSDRIEPLTFYLDPQWQLALNPSERKYCGSGFHGSDNVFSNMQALFVGYGPGFKHGIEADTFENIEVYNLMCDLLNLTPAPNNGTHGSLNHLLKNPVYTPKHPKEVHPLVQCPFTRNPRDNLGCSCNPSILPIEDFQTQFNLTVAEEKIIKHETLPYGRPRVLQKENTICLLSQHQFMSGYSQDILMPLWTSYTVDRNDSFSTEDFSNCLYQDFRIPLSPVHKCSFYKNNTKVSYGFLSPPQLNKNSSGIYSEALLTTNIVPMYQSFQVIWRYFHDTLLRKYAEERNGVNVVSGPVFDFDYDGRCDSLENLRQKRRVIRNQEILIPTHFFIVLTSCKDTSQTPLHCENLDTLAFILPHRTDNSESCVHGKHDSSWVEELLMLHRARITDVEHITGLSFYQQRKEPVSDILKLKTHLPTFSQED 0ECAA063801CAFEB insr-enpp1 1 3 unspecified role unspecified role bait bait cell lysate cell lysate 4 unspecified role unspecified role prey prey cell lysate cell lysate lys173gln disease aa variant disease causing amino-acid variant disease aa variant full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain The glutamine-containing variant is more strongly associated with IR than the lysine-containing variant, detected by peroxidase-streptavidin method by measuring peroxidase activity which was determined colourimetrically P<0.01. proximity proximity close-contact colocalization neighbourhood interaction Figure 2 insr-enpp1-1 2 4 unspecified role unspecified role prey prey cell lysate cell lysate lys173gln disease aa variant disease causing amino-acid variant disease aa variant full dna sequence full identification by DNA sequencing full dna sequence certain certain sequence position certain certain certain sequence position certain The glutamine-containing variant is more strongly associated with IR than the lysine-containing variant as measured by scanning densitometry of western blots P<0.001. 3 unspecified role unspecified role bait bait cell lysate cell lysate physical association physical association Figure 3 A & B